##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2165693 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45532815592976 33.0 31.0 34.0 31.0 34.0 2 32.68189535635938 34.0 31.0 34.0 31.0 34.0 3 32.748601948660315 34.0 31.0 34.0 31.0 34.0 4 36.2210045468125 37.0 35.0 37.0 35.0 37.0 5 36.14217389075922 37.0 35.0 37.0 35.0 37.0 6 36.195797834688484 37.0 37.0 37.0 35.0 37.0 7 36.16392351085773 37.0 37.0 37.0 35.0 37.0 8 36.15896020350068 37.0 37.0 37.0 35.0 37.0 9 37.84529894126268 39.0 38.0 39.0 35.0 39.0 10-11 37.81234667148114 39.0 38.0 39.0 35.0 39.0 12-13 37.73748541459939 39.0 38.0 39.0 35.0 39.0 14-15 38.97504170720411 40.0 38.0 41.0 35.5 41.0 16-17 38.88691772102509 40.0 38.0 41.0 35.0 41.0 18-19 39.1393895626019 40.0 38.5 41.0 36.0 41.0 20-21 39.1109605562746 40.0 39.0 41.0 36.0 41.0 22-23 39.008556845314644 40.0 38.5 41.0 35.0 41.0 24-25 38.908848114668146 40.0 38.0 41.0 35.0 41.0 26-27 38.74804531390183 40.0 38.0 41.0 35.0 41.0 28-29 38.623956165532235 40.0 38.0 41.0 34.5 41.0 30-31 38.38058879998227 40.0 38.0 41.0 34.0 41.0 32-33 38.14691348219715 40.0 38.0 41.0 34.0 41.0 34-35 38.0493798059097 40.0 38.0 41.0 33.0 41.0 36-37 37.991984320954074 40.0 38.0 41.0 33.0 41.0 38-39 37.79894749625178 40.0 37.0 41.0 33.0 41.0 40-41 37.58075544410034 40.0 37.0 41.0 33.0 41.0 42-43 37.323041400604794 40.0 37.0 41.0 32.0 41.0 44-45 37.04422418135904 40.0 36.0 41.0 31.0 41.0 46-47 36.66560772925803 39.5 36.0 41.0 30.5 41.0 48-49 36.245230741383935 39.0 35.5 41.0 30.0 41.0 50-51 35.8567093766291 39.0 35.0 40.0 28.5 41.0 52-53 35.32121311746402 38.0 35.0 40.0 27.0 41.0 54-55 34.94449744261998 38.0 34.0 40.0 26.0 41.0 56-57 34.566880901402 38.0 34.0 40.0 26.0 41.0 58-59 34.06863415082378 37.5 33.5 40.0 23.5 41.0 60-61 33.589615425639735 37.0 33.0 40.0 22.5 41.0 62-63 33.037805450726395 36.0 32.0 39.0 21.0 40.5 64-65 32.47260299590016 36.0 31.5 39.0 18.5 40.0 66-67 31.913732694338485 35.0 31.0 38.5 15.5 40.0 68-69 31.297972750523734 35.0 30.0 38.0 10.0 40.0 70-71 30.68457001985046 34.5 29.5 37.5 7.5 39.5 72-73 30.01838834036034 34.0 29.0 37.0 4.0 39.0 74-75 29.354317763413373 34.0 28.0 36.0 2.0 39.0 76-77 28.06478619084053 32.5 26.5 35.0 2.0 37.0 78-79 28.713616149657405 33.5 28.0 35.0 2.0 37.0 80-81 28.434880890320095 34.0 27.5 35.0 2.0 37.0 82-83 27.873292982892774 33.0 26.5 35.0 2.0 36.5 84-85 27.216101497303633 33.0 26.0 35.0 2.0 36.0 86-87 26.504209507072332 32.5 24.5 35.0 2.0 35.5 88-89 25.707598214520708 32.0 22.0 34.5 2.0 35.0 90-91 25.051755950635663 31.5 19.0 34.0 2.0 35.0 92-93 24.118918747948115 31.0 11.0 34.0 2.0 35.0 94-95 23.223003445086633 31.0 2.0 34.0 2.0 35.0 96-97 22.180716518915652 30.0 2.0 34.0 2.0 35.0 98-99 20.784325617712206 29.0 2.0 34.0 2.0 35.0 100-101 16.937298592182735 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12525430276779304 1101 2 0.10459707761895487 1101 3 0.1077160571634792 1101 4 0.01649050358193449 1101 5 0.016544562534477336 1101 6 0.05402817772323942 1101 7 0.09277124512387047 1101 8 0.1646790721055993 1101 9 0.03322692575200392 1101 10-11 0.06275702279567241 1101 12-13 0.24254596943764284 1101 14-15 0.36256964631505184 1101 16-17 0.3822351166326996 1101 18-19 0.3056184865870293 1101 20-21 0.2772575023087711 1101 22-23 0.23261117724916147 1101 24-25 0.33329480082898755 1101 26-27 0.47081911179630964 1101 28-29 0.34487397799036046 1101 30-31 0.5268250381384902 1101 32-33 0.46661950510357997 1101 34-35 0.2821551877375086 1101 36-37 0.307109320176842 1101 38-39 0.502893702313024 1101 40-41 0.5967922526644927 1101 42-43 0.6853810211254441 1101 44-45 0.7604761948703711 1101 46-47 0.7824568831695515 1101 48-49 0.9436389822130664 1101 50-51 1.1157612177461047 1101 52-53 1.0392091228068097 1101 54-55 1.1370938512186015 1101 56-57 1.1454242169044875 1101 58-59 1.4072293892517322 1101 60-61 1.369634472063133 1101 62-63 1.455468593722209 1101 64-65 1.4338657646067432 1101 66-67 1.7751394689119309 1101 68-69 1.8782894423269347 1101 70-71 1.9057278453292525 1101 72-73 1.9620583006167145 1101 74-75 2.129811136866355 1101 76-77 2.1565521325773815 1101 78-79 2.0912658627793483 1101 80-81 2.2371388894583895 1101 82-83 2.2831662705308986 1101 84-85 2.3525724403218398 1101 86-87 2.598070534094152 1101 88-89 2.9055562990892234 1101 90-91 3.164437847093776 1101 92-93 3.5102712255932538 1101 94-95 3.7429459038074917 1101 96-97 3.8319670285383403 1101 98-99 4.054694070768097 1101 100-101 4.100993861679047 1102 1 0.23725557733308733 1102 2 0.18450645464641013 1102 3 0.25863758627372135 1102 4 0.1578533681789267 1102 5 0.02694699387129873 1102 6 0.06097904484175132 1102 7 0.06244567881656593 1102 8 0.13534278932695543 1102 9 0.1364586619676018 1102 10-11 0.21306858959071207 1102 12-13 0.25424741057067024 1102 14-15 0.3721944547851592 1102 16-17 0.3970467616501594 1102 18-19 0.41639667311528683 1102 20-21 0.3436280741146547 1102 22-23 0.32996587910671593 1102 24-25 0.3632421962525214 1102 26-27 0.34300598055289555 1102 28-29 0.374260587692703 1102 30-31 0.3807972990884778 1102 32-33 0.42044929772214346 1102 34-35 0.5312605188253912 1102 36-37 0.4620075390063576 1102 38-39 0.5394649836422474 1102 40-41 0.5423672794671788 1102 42-43 0.6380426539494053 1102 44-45 0.6216567092057872 1102 46-47 0.6179669278483502 1102 48-49 0.7695291073229242 1102 50-51 0.7988428014279947 1102 52-53 1.000376868482718 1102 54-55 1.0379092624154467 1102 56-57 1.1467830438442945 1102 58-59 1.2143006958882125 1102 60-61 1.1080676195031813 1102 62-63 1.2201741208262789 1102 64-65 1.147608810863801 1102 66-67 1.4453764713368287 1102 68-69 1.536394205151968 1102 70-71 1.610614790927869 1102 72-73 1.963733468219747 1102 74-75 2.0828598556291524 1102 76-77 1.9890839737030461 1102 78-79 2.0714433816562625 1102 80-81 2.1746777130852415 1102 82-83 2.2482330586723798 1102 84-85 2.2457186035122128 1102 86-87 2.235012969296079 1102 88-89 2.5781586669278447 1102 90-91 2.9585225067572374 1102 92-93 2.9141321346297815 1102 94-95 3.2909350700025257 1102 96-97 3.5652119015189356 1102 98-99 4.0863778888503575 1102 100-101 4.161719765723145 1103 1 0.16948457144675189 1103 2 0.06560115694364299 1103 3 0.10846438212723797 1103 4 0.06197901281090168 1103 5 0.0020212242197885644 1103 6 -0.009793732154648183 1103 7 0.06999894234732551 1103 8 0.03166122449911768 1103 9 0.12167119517591374 1103 10-11 0.19522400449929478 1103 12-13 0.1935901464148131 1103 14-15 0.303225345028423 1103 16-17 0.3435064949418205 1103 18-19 0.15335427065657115 1103 20-21 0.20562474026039723 1103 22-23 0.22214304220511139 1103 24-25 0.3014584540011285 1103 26-27 0.312418518970361 1103 28-29 0.2575073189527899 1103 30-31 0.40245107311566386 1103 32-33 0.37306233621774254 1103 34-35 0.36749144032354053 1103 36-37 0.3915342879775565 1103 38-39 0.34234433979164436 1103 40-41 0.49475892920411724 1103 42-43 0.5164253439530526 1103 44-45 0.5351327731741193 1103 46-47 0.6934897960048332 1103 48-49 0.9240389265655153 1103 50-51 0.9333429837481475 1103 52-53 0.996382103675721 1103 54-55 1.1718331828196753 1103 56-57 1.1744664058279355 1103 58-59 1.3208245016911988 1103 60-61 1.2912179815389209 1103 62-63 1.359424394306508 1103 64-65 1.3730769829740268 1103 66-67 1.3611413043836507 1103 68-69 1.6694195476533942 1103 70-71 1.7855086545523875 1103 72-73 2.013086882815781 1103 74-75 1.8786482600673473 1103 76-77 1.9990106549553275 1103 78-79 2.0849090273300668 1103 80-81 2.0466694305410975 1103 82-83 2.1810350589849286 1103 84-85 2.398134996698648 1103 86-87 2.605419152553882 1103 88-89 2.7034451700272903 1103 90-91 2.8082906997040524 1103 92-93 3.0529852106312383 1103 94-95 3.2425030233285668 1103 96-97 3.544544609754599 1103 98-99 3.6487964959243797 1103 100-101 3.4598067063909568 1104 1 0.2996040602750938 1104 2 0.2770178420183811 1104 3 0.24111088991180907 1104 4 0.1506875026128327 1104 5 0.0868701553245188 1104 6 0.1740967521003114 1104 7 0.17708379945980113 1104 8 0.23913040120840634 1104 9 0.2152915716823074 1104 10-11 0.311084397695673 1104 12-13 0.3101572049688386 1104 14-15 0.44889249439560075 1104 16-17 0.40960118196372264 1104 18-19 0.41435564921648194 1104 20-21 0.372783311466911 1104 22-23 0.37487889048988876 1104 24-25 0.46543651976293887 1104 26-27 0.47471991634741073 1104 28-29 0.439801414462039 1104 30-31 0.5232448290982887 1104 32-33 0.599749026773992 1104 34-35 0.6226850727675597 1104 36-37 0.6689326549772048 1104 38-39 0.7262661177657748 1104 40-41 0.8489665026932229 1104 42-43 0.851520227327704 1104 44-45 0.7942099970735157 1104 46-47 0.9434795692992282 1104 48-49 0.8927748648453004 1104 50-51 0.9863669183299564 1104 52-53 1.2740386949920222 1104 54-55 1.3483215775541524 1104 56-57 1.365768183638437 1104 58-59 1.437746938265171 1104 60-61 1.433936771678738 1104 62-63 1.6620592027398615 1104 64-65 1.7055318422954642 1104 66-67 1.7986592011184683 1104 68-69 2.0468703776528265 1104 70-71 2.1235089449908315 1104 72-73 2.3666015969998107 1104 74-75 2.4075519981327815 1104 76-77 2.3990047234660743 1104 78-79 2.6410730718694104 1104 80-81 2.527494357522418 1104 82-83 2.713178617387598 1104 84-85 2.9074617522149317 1104 86-87 2.960432079109772 1104 88-89 3.2151762240324757 1104 90-91 3.4540713655034914 1104 92-93 3.856125218321722 1104 94-95 3.734133998792654 1104 96-97 4.013806200702735 1104 98-99 4.053074020079858 1104 100-101 3.70678062420097 1105 1 0.19566752362553075 1105 2 0.16216264780615575 1105 3 0.1382947618385515 1105 4 0.07840032940269737 1105 5 -0.059101312507550574 1105 6 -9.569200218493279E-4 1105 7 0.11114794841893882 1105 8 0.0765929614533718 1105 9 0.23330623092606828 1105 10-11 0.25609698171421513 1105 12-13 0.2272789266601194 1105 14-15 0.35703448512051494 1105 16-17 0.281023282579703 1105 18-19 0.2953680405286221 1105 20-21 0.2813440028471277 1105 22-23 0.37243023609381254 1105 24-25 0.24950221812028417 1105 26-27 0.38210488079356253 1105 28-29 0.37473213699484376 1105 30-31 0.35944039317123355 1105 32-33 0.44658605138715757 1105 34-35 0.45635354416034346 1105 36-37 0.40625579359313946 1105 38-39 0.45373883625271816 1105 40-41 0.43944930001547533 1105 42-43 0.4513841465885591 1105 44-45 0.6110239211278312 1105 46-47 0.6200338577913627 1105 48-49 0.7573176374352855 1105 50-51 0.682849251801116 1105 52-53 0.8569613974523662 1105 54-55 0.9347792078077006 1105 56-57 0.8074390304523433 1105 58-59 0.9888320496136416 1105 60-61 0.8949999106239304 1105 62-63 1.0285723740771573 1105 64-65 1.1099828759861623 1105 66-67 1.180187856754067 1105 68-69 1.414863708461926 1105 70-71 1.5573036439185444 1105 72-73 1.715778818134762 1105 74-75 1.7130858190746387 1105 76-77 1.7002505848782121 1105 78-79 1.769099482330173 1105 80-81 2.017041003607911 1105 82-83 2.1421954806503045 1105 84-85 2.2511294101736574 1105 86-87 2.172699996205349 1105 88-89 2.4142465366913477 1105 90-91 2.5765413677017044 1105 92-93 2.584904273169453 1105 94-95 2.766450087646767 1105 96-97 3.0911107610658384 1105 98-99 3.1062031265903336 1105 100-101 2.7530243503487064 1106 1 0.1355349377254953 1106 2 0.12230748085838172 1106 3 0.1320482485515555 1106 4 0.11480813256162747 1106 5 -0.024930360889861447 1106 6 0.015387440094720262 1106 7 0.1254433553177634 1106 8 0.15234043355733462 1106 9 0.24020328685969616 1106 10-11 0.1858496684041171 1106 12-13 0.12006686745282735 1106 14-15 0.05966571358904105 1106 16-17 0.0397120084008904 1106 18-19 -0.04593471186748843 1106 20-21 -0.012476354248171617 1106 22-23 -0.04996449660627178 1106 24-25 0.04055369434450995 1106 26-27 -0.03809421226523568 1106 28-29 0.011252725714982148 1106 30-31 0.07380048179776821 1106 32-33 0.16123194570401722 1106 34-35 0.13506918111936272 1106 36-37 0.3081675135124229 1106 38-39 0.2583175947547076 1106 40-41 0.25235449215605854 1106 42-43 0.17101435244317287 1106 44-45 0.2614780902353857 1106 46-47 0.3981416541514875 1106 48-49 0.31561687587662846 1106 50-51 0.5090463294350229 1106 52-53 0.49376336798655274 1106 54-55 0.6081593007370429 1106 56-57 0.5819928243220573 1106 58-59 0.7631587123624612 1106 60-61 0.7844009084641428 1106 62-63 0.7267437531810046 1106 64-65 0.7982898259423408 1106 66-67 1.0527137457436453 1106 68-69 1.1117193336301519 1106 70-71 0.9355506526796873 1106 72-73 1.401022699448216 1106 74-75 1.5619715610611387 1106 76-77 1.5793913330627944 1106 78-79 1.719654166563199 1106 80-81 1.49500288190195 1106 82-83 2.017552184778662 1106 84-85 2.0152005963964967 1106 86-87 2.139964825271427 1106 88-89 2.33075949306075 1106 90-91 2.126126035571044 1106 92-93 2.1741739051681357 1106 94-95 2.525063374735847 1106 96-97 3.023757012086822 1106 98-99 3.368753081466796 1106 100-101 3.365335509041067 1107 1 0.23007773165677037 1107 2 0.16026910074951672 1107 3 0.16584695627226154 1107 4 0.08863929378152591 1107 5 0.003085506893263812 1107 6 0.07902065694709393 1107 7 0.1480463384023878 1107 8 0.17546355173056583 1107 9 0.08665629521848928 1107 10-11 0.21773358304335488 1107 12-13 0.21609085593659927 1107 14-15 0.2942232874152637 1107 16-17 0.28434124731871435 1107 18-19 0.20977899039547054 1107 20-21 0.24659075416702336 1107 22-23 0.2538497968524851 1107 24-25 0.37093837453468836 1107 26-27 0.3746879243911039 1107 28-29 0.3860549278643859 1107 30-31 0.42935081768782624 1107 32-33 0.46380130315402823 1107 34-35 0.49320096210586684 1107 36-37 0.43327190682245487 1107 38-39 0.5520080264668294 1107 40-41 0.6495499750392213 1107 42-43 0.8479582657637579 1107 44-45 1.026920058543773 1107 46-47 0.9727398314809221 1107 48-49 0.9876652923949507 1107 50-51 0.9781404152792916 1107 52-53 1.0008254838374953 1107 54-55 1.0567753567944251 1107 56-57 1.1382896639519657 1107 58-59 1.302580922863335 1107 60-61 1.2786505769695964 1107 62-63 1.4262613462009384 1107 64-65 1.4726761031670108 1107 66-67 1.6357439721855513 1107 68-69 1.8689636799082585 1107 70-71 1.8285145435831822 1107 72-73 1.8283274106999805 1107 74-75 1.7438036453376746 1107 76-77 1.798561882428011 1107 78-79 1.7585644835525684 1107 80-81 1.9525070210051219 1107 82-83 2.2891661786103015 1107 84-85 2.36194938580039 1107 86-87 2.4889777590530997 1107 88-89 2.5610264102713636 1107 90-91 2.667237769379092 1107 92-93 2.788519835294217 1107 94-95 3.057885455569238 1107 96-97 3.1975352440670726 1107 98-99 3.4292261113635725 1107 100-101 3.171740862769557 1108 1 0.23655406945381685 1108 2 0.2311945499437158 1108 3 0.17330749417619984 1108 4 0.10450975573456844 1108 5 0.07033478991383646 1108 6 0.1426596380459344 1108 7 0.14741310066631286 1108 8 0.1901277569560449 1108 9 0.26746966227431557 1108 10-11 0.16990202239932017 1108 12-13 0.21190047813924906 1108 14-15 0.3499834926106331 1108 16-17 0.2833278787574187 1108 18-19 0.2590863807408965 1108 20-21 0.2343555284596448 1108 22-23 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-0.3541909565278871 2316 50-51 -0.15314529711249492 2316 52-53 -0.17098675018390708 2316 54-55 0.0582693113742252 2316 56-57 0.17303177242482803 2316 58-59 0.34998974440411956 2316 60-61 0.35590286057404796 2316 62-63 0.3547520460643838 2316 64-65 0.5853582280791869 2316 66-67 0.7468351970460247 2316 68-69 0.9212413184822772 2316 70-71 1.0965580016695924 2316 72-73 1.0854201734304958 2316 74-75 1.4724209543302358 2316 76-77 1.6349750988301892 2316 78-79 1.762130751168474 2316 80-81 1.6708737963832938 2316 82-83 1.8580289502233391 2316 84-85 1.9611022315228617 2316 86-87 2.1981968591339722 2316 88-89 2.5384356295371653 2316 90-91 2.6028444325988076 2316 92-93 2.6877802324889295 2316 94-95 3.0279906206914013 2316 96-97 3.52047801143771 2316 98-99 3.8538787648522863 2316 100-101 4.293184538278975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 13.0 5 40.0 6 78.0 7 204.0 8 507.0 9 997.0 10 1806.0 11 3088.0 12 4726.0 13 7155.0 14 9565.0 15 13202.0 16 17579.0 17 24067.0 18 24928.0 19 20416.0 20 19529.0 21 20364.0 22 22412.0 23 24868.0 24 27708.0 25 31141.0 26 35371.0 27 41223.0 28 48873.0 29 58519.0 30 70130.0 31 83799.0 32 101305.0 33 126778.0 34 164643.0 35 224888.0 36 312594.0 37 391998.0 38 220306.0 39 10863.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62136877202817 19.13036612299158 15.307478945538449 22.940786159441803 2 15.42109183742742 19.869314972713532 40.56948524471 24.14010794514905 3 19.08302268570402 23.87237565667746 30.262689453495668 26.781912204122854 4 12.800742505679613 17.0005633438614 37.0374577492104 33.16123640124859 5 13.39166139276348 37.42498845442816 34.6939001935588 14.489449959249564 6 32.22234176312155 36.49991019041018 16.963623191283343 14.314124855184923 7 28.42799048618618 33.0717234621897 20.29807548900052 18.202210562623602 8 26.21321835206027 35.50782523412905 19.272784697319924 19.006171716490755 9 26.040810031708094 16.8663794914607 20.228536546962104 36.8642739298691 10-11 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26.672617610020488 29.454214140679007 21.70291800951566 36-37 22.23431746542601 26.26494246730186 29.47053848869282 22.030201578579305 38-39 21.820850020618117 26.521711825657068 29.40515457586013 22.252283577864684 40-41 22.314551638309023 26.35159628629944 29.394041547082768 21.93981052830877 42-43 21.964381572248705 26.39974972207375 29.284710766222176 22.35115793945537 44-45 22.0101328809308 26.68319924566741 29.335369083169137 21.971298790232648 46-47 22.348366088822377 26.97270573437694 28.539848445740002 22.139079731060683 48-49 22.124257685646118 26.98039380466206 28.54645141301191 22.348897096679906 50-51 22.294826644404356 27.196190780503056 28.424689002550224 22.084293572542368 52-53 22.616894453646015 27.003434928219285 28.28491849952879 22.094752118605914 54-55 22.194974079890365 27.129699361820904 28.426004978544974 22.249321579743757 56-57 22.231290399885857 27.247606193490952 28.28000090502209 22.241102501601105 58-59 22.245258215268738 26.971851504345263 28.21374035932147 22.569149921064525 60-61 21.95784444055552 27.255640573248378 28.402479021726535 22.38403596446957 62-63 22.06928682874258 27.215283976076016 28.490787937163763 22.22464125801764 64-65 21.89423893414256 27.243196519543627 28.29108280813578 22.571481738178033 66-67 21.736493462597636 27.320528496203522 28.24974650134922 22.693231539849616 68-69 22.2359977706699 27.043163949497455 28.322881616938478 22.397956662894167 70-71 22.097822729260333 27.067848490067615 27.932075321848483 22.902253458823573 72-73 22.102260593320615 27.148798825318 27.9186911345909 22.83024944677049 74-75 22.11089014001523 27.49482498211889 27.699747840529565 22.694537037336318 76-77 22.10788879125527 27.10494977820033 28.092739829698854 22.694421600845548 78-79 22.190702929731962 27.26907738077373 27.98330603645115 22.55691365304316 80-81 22.291858676118302 27.366310629581676 27.712227777541777 22.629602916758245 82-83 22.342417471393023 27.365128970933807 27.79458571888349 22.49786783878968 84-85 22.298705994317515 27.49052110707374 27.658580689609952 22.552192208998793 86-87 22.324065751721843 27.42082390286621 27.587495841282323 22.667614504129627 88-89 22.29256408918531 27.60183460906047 27.297613281291483 22.807988020462734 90-91 22.215485676593016 27.49154000434515 27.454807103787417 22.83816721527442 92-93 22.286469042472778 27.22962118822936 27.511193876509736 22.972715892788127 94-95 22.112701159721695 27.188007343611186 27.552509775166552 23.146781721500563 96-97 22.265341639227387 26.902752978148776 27.631619653958023 23.200285728665815 98-99 22.03451735772337 27.054804166610875 27.349421178347992 23.561257297317763 100-101 22.451658660761243 26.567454389888134 27.452829186777628 23.528057762572995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 791.0 1 529.5 2 367.0 3 493.5 4 394.5 5 333.0 6 716.0 7 1685.0 8 2858.5 9 3355.0 10 2786.5 11 2141.0 12 2139.5 13 2255.5 14 2392.0 15 2609.0 16 2827.0 17 3042.5 18 3282.5 19 3634.0 20 4153.0 21 5004.0 22 6060.0 23 7367.5 24 9194.0 25 11464.0 26 14311.5 27 17716.0 28 20934.0 29 23818.5 30 27029.0 31 31551.0 32 37419.5 33 40644.5 34 42265.0 35 45212.0 36 49420.0 37 53425.5 38 56275.5 39 58926.0 40 62121.5 41 65671.5 42 67693.5 43 70024.5 44 73240.5 45 76527.5 46 78032.5 47 79991.5 48 81547.0 49 79632.5 50 77212.0 51 75373.5 52 73794.0 53 73199.0 54 73397.0 55 69762.5 56 63588.0 57 57564.0 58 52821.0 59 47049.0 60 38082.5 61 31067.0 62 26015.0 63 19755.0 64 14108.0 65 10520.0 66 7603.5 67 5627.0 68 4170.5 69 2998.0 70 2262.0 71 1520.0 72 1017.5 73 767.5 74 535.0 75 354.5 76 250.0 77 158.0 78 93.0 79 51.5 80 26.0 81 16.0 82 9.5 83 4.5 84 3.5 85 3.0 86 2.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015422315166554077 3 0.0025857773931946956 4 0.0024472536042735513 5 0.11663703027160359 6 0.0 7 0.0 8 3.2322217414933695E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.770475778422888E-4 24-25 4.3865866491695735E-4 26-27 0.0054255150660781565 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009696665224480108 36-37 5.310078575310536E-4 38-39 0.005633300749459873 40-41 0.009142570068795531 42-43 0.004640546928858338 44-45 0.0032322217414933693 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.617459630704814E-5 68-69 9.234919261409627E-4 70-71 0.0 72-73 2.308729815352407E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.617459630704814E-5 82-83 0.002308729815352407 84-85 0.004248062860248428 86-87 0.0028397376728834605 88-89 0.0 90-91 0.002747388480269364 92-93 0.0 94-95 4.617459630704814E-5 96-97 9.234919261409628E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2165693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.00436759666453 #Duplication Level Percentage of deduplicated Percentage of total 1 74.58931232184443 41.0273795373315 2 14.427510266621868 15.871521564198416 3 4.826239684089266 7.963927850797684 4 2.1166235906479143 4.656941681750795 5 1.164906773118363 3.203748018222337 6 0.6718743781615706 2.217361516510765 7 0.4407625230371014 1.6970704687976228 8 0.30512605859923625 1.3426612712411035 9 0.21814529614476771 1.0799049652767043 >10 1.1308002676008178 11.126688244120619 >50 0.06995082360992937 2.6411674204170925 >100 0.033551002676878994 3.4320455289477954 >500 0.003352912159853012 1.330855556521736 >1k 0.0015926332759301407 1.3302561758795919 >5k 2.514684119889696E-4 1.07847019998612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9642 0.44521545759255815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8400 0.38786660897920433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5349 0.2469879156464005 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2223 0.102646127590568 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2174 0.10038357237152265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 4.6174596307048135E-5 0.0 0.0 0.0 0.0 20-21 4.6174596307048135E-5 0.0 0.0 0.0 0.0 22-23 9.234919261409627E-5 0.0 0.0 2.3087298153524067E-5 0.0 24-25 1.8469838522819254E-4 0.0 0.0 4.155713667634332E-4 0.0 26-27 1.8469838522819254E-4 0.0 0.0 9.234919261409628E-4 0.0 28-29 2.077856833817166E-4 0.0 0.0 0.003670880406410327 0.0 30-31 2.308729815352407E-4 0.0 0.0 0.013275196438276338 0.0 32-33 2.308729815352407E-4 0.0 0.0 0.03359201881337752 0.0 34-35 2.308729815352407E-4 0.0 0.0 0.06420577616495043 0.0 36-37 2.770475778422888E-4 0.0 0.0 0.1101264121923098 0.0 38-39 2.770475778422888E-4 0.0 0.0 0.18202025864238375 0.0 40-41 2.770475778422888E-4 0.0 0.0 0.25569182705027904 0.0 42-43 3.4630947230286104E-4 0.0 0.0 0.33051776036585057 2.3087298153524067E-5 44-45 3.693967704563851E-4 0.0 0.0 0.4189421122938477 4.6174596307048135E-5 46-47 4.617459630704814E-4 0.0 0.0 0.522303946127175 4.6174596307048135E-5 48-49 4.617459630704814E-4 0.0 0.0 0.6309989458339663 4.6174596307048135E-5 50-51 4.617459630704814E-4 0.0 0.0 0.7443575797677695 4.6174596307048135E-5 52-53 4.617459630704814E-4 0.0 0.0 0.8534219762450173 9.234919261409627E-5 54-55 4.617459630704814E-4 0.0 0.0 0.9740761963953339 9.234919261409627E-5 56-57 4.617459630704814E-4 0.0 0.0 1.111260922023574 9.234919261409627E-5 58-59 4.617459630704814E-4 0.0 0.0 1.255140964116336 1.1543649076762035E-4 60-61 4.617459630704814E-4 0.0 0.0 1.409641163359719 1.385237889211444E-4 62-63 4.617459630704814E-4 0.0 0.0 1.5588774586240985 1.385237889211444E-4 64-65 4.617459630704814E-4 0.0 0.0 1.7142088006010086 1.385237889211444E-4 66-67 5.079205593775295E-4 0.0 0.0 1.892211869364679 1.385237889211444E-4 68-69 5.540951556845776E-4 0.0 0.0 2.0849446343502978 1.385237889211444E-4 70-71 5.540951556845776E-4 0.0 0.0 2.2899367546554386 1.8469838522819254E-4 72-73 5.540951556845776E-4 0.0 0.0 2.4958754541848727 1.8469838522819254E-4 74-75 5.540951556845776E-4 0.0 0.0 2.699990257160179 1.8469838522819254E-4 76-77 5.540951556845776E-4 0.0 0.0 2.9189732801463553 1.8469838522819254E-4 78-79 5.540951556845776E-4 0.0 0.0 3.1665845528429006 1.8469838522819254E-4 80-81 5.540951556845776E-4 0.0 0.0 3.4240771891491546 2.077856833817166E-4 82-83 5.540951556845776E-4 0.0 0.0 3.6868568167325657 2.308729815352407E-4 84-85 5.771824538381017E-4 0.0 0.0 3.9567242448491085 2.308729815352407E-4 86-87 6.002697519916258E-4 0.0 0.0 4.232848330765256 2.308729815352407E-4 88-89 6.002697519916258E-4 0.0 0.0 4.521231772000926 2.308729815352407E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5095 0.0 52.74284 1 GTATCAA 8610 0.0 39.165375 1 ATCAACG 10500 0.0 32.065125 3 TATCAAC 10810 0.0 31.282623 2 TCAACGC 10760 0.0 31.244677 4 CAACGCA 10840 0.0 31.012653 5 AACGCAG 11320 0.0 29.705303 6 ACCGTCG 100 4.9587175E-5 28.496954 8 CCGTCGT 90 8.9557027E-4 26.386066 9 ACGCAGA 12835 0.0 26.19899 7 CGCAGAG 12785 0.0 26.152855 8 GTGGTAT 2440 0.0 24.170805 1 TGGTATC 2300 0.0 23.987743 2 GCAGAGT 14270 0.0 23.331423 9 GAGTACT 9390 0.0 22.40708 12-13 TACATGG 7015 0.0 21.831694 2 GTACATG 7285 0.0 21.74072 1 CAGAGTA 13675 0.0 21.602808 10-11 GTACTTT 9740 0.0 21.577518 14-15 TACCGTC 200 5.3961776E-7 21.372715 7 >>END_MODULE