FastQCFastQC Report
Thu 26 May 2016
SRR938082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123383
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97990.4614805713335748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79720.3754386278876679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50150.23617971887313782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.052.7527161
GTATCAA84750.040.373381
ATCAACG105500.032.2576073
TATCAAC107550.031.5985552
TCAACGC107400.031.5541764
CAACGCA108150.031.2914055
AACGCAG110600.030.6784046
ACGCAGA124750.027.036087
CGCAGAG125400.026.7822958
GCAGAGT133650.024.4525959
GTACATG73650.024.0390241
TACATGG72800.023.3081842
GAGTACT87200.022.8539812-13
GTATACG1252.2470171E-422.81464
TGGTATC21450.022.6018752
TAAGGTG13050.022.5814975
GTGGTAT24800.022.3214261
CAGAGTA127800.022.26364710-11
AGAGTAC120000.021.61283510-11
GTACTTT92100.021.51191514-15
Produced by FastQC (version 0.11.5)