##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938082_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2123383 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6736966435165 33.0 31.0 34.0 30.0 34.0 2 31.879266717309125 33.0 31.0 34.0 30.0 34.0 3 31.953881141555716 34.0 31.0 34.0 30.0 34.0 4 35.486551413475574 37.0 35.0 37.0 33.0 37.0 5 35.49180293898934 37.0 35.0 37.0 33.0 37.0 6 35.50419542776786 37.0 35.0 37.0 33.0 37.0 7 35.44221414601134 37.0 35.0 37.0 33.0 37.0 8 35.40971835980603 37.0 35.0 37.0 33.0 37.0 9 37.01789031936302 39.0 37.0 39.0 33.0 39.0 10-11 36.91863102417228 39.0 37.0 39.0 33.0 39.0 12-13 36.80848203079708 39.0 37.0 39.0 33.0 39.0 14-15 37.95280079006001 40.0 38.0 41.0 33.0 41.0 16-17 37.789036881240925 40.0 38.0 41.0 32.5 41.0 18-19 37.9434426102121 40.0 38.0 41.0 33.0 41.0 20-21 37.91992777562974 40.0 38.0 41.0 33.0 41.0 22-23 37.822625499026785 40.0 38.0 41.0 32.5 41.0 24-25 37.710718226528144 40.0 38.0 41.0 32.0 41.0 26-27 37.5226087333279 40.0 37.5 41.0 32.0 41.0 28-29 37.3036025530957 40.0 37.0 41.0 31.5 41.0 30-31 37.07070533201029 40.0 37.0 41.0 31.0 41.0 32-33 36.79536381331112 39.5 36.0 41.0 30.0 41.0 34-35 36.54957607741986 39.0 36.0 41.0 30.0 41.0 36-37 36.27427976959409 39.0 35.0 41.0 30.0 41.0 38-39 35.96018028777662 39.0 35.0 40.0 28.5 41.0 40-41 35.72624345207623 39.0 35.0 40.0 27.5 41.0 42-43 35.522959117596784 39.0 35.0 40.0 26.5 41.0 44-45 35.23029665397152 38.5 35.0 40.0 25.5 41.0 46-47 34.8257968534174 38.0 34.0 40.0 24.0 41.0 48-49 34.40952409433437 38.0 34.0 40.0 23.0 41.0 50-51 33.710934861963196 37.5 33.0 39.5 22.0 40.5 52-53 33.84687854240144 38.0 33.0 39.5 22.0 40.5 54-55 33.940910565828204 38.0 33.5 40.0 21.0 41.0 56-57 33.63402551494478 38.0 33.0 40.0 18.5 41.0 58-59 33.30458800885191 37.5 33.0 40.0 17.5 41.0 60-61 32.87487419839002 37.0 32.5 40.0 13.0 41.0 62-63 32.31384988012054 36.0 31.5 39.5 8.0 41.0 64-65 31.7775434295179 36.0 31.0 39.0 6.5 40.5 66-67 31.225473454388585 35.0 30.5 39.0 2.0 40.0 68-69 30.60897680729289 35.0 29.5 38.0 2.0 40.0 70-71 30.034435379768983 35.0 29.0 37.5 2.0 40.0 72-73 29.37009997725328 34.0 28.0 37.0 2.0 39.0 74-75 28.653790908187545 34.0 26.5 36.0 2.0 39.0 76-77 27.964990771801414 34.0 26.0 36.0 2.0 38.5 78-79 27.209749724849452 33.0 25.0 35.0 2.0 37.5 80-81 26.536035656308826 32.5 23.5 35.0 2.0 37.0 82-83 25.777797269734194 32.0 20.5 35.0 2.0 36.0 84-85 25.093478661174174 31.5 18.5 35.0 2.0 36.0 86-87 24.37330170769946 31.0 12.0 34.0 2.0 35.0 88-89 23.53978156554894 30.5 4.5 34.0 2.0 35.0 90-91 22.68320929384854 30.0 2.0 34.0 2.0 35.0 92-93 21.765707128671558 29.0 2.0 34.0 2.0 35.0 94-95 20.79553641523927 29.0 2.0 34.0 2.0 35.0 96-97 19.575795558314255 26.5 2.0 33.5 2.0 35.0 98-99 18.09279884976003 24.5 2.0 33.0 2.0 35.0 100-101 14.585280893743615 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24406074580245019 1101 2 0.3151494110516957 1101 3 0.20886217886957326 1101 4 0.23131142593608445 1101 5 0.06977038827265147 1101 6 0.30807685952183306 1101 7 0.23053662148495846 1101 8 0.1408052081877429 1101 9 0.27460797853942864 1101 10-11 0.35300359565724193 1101 12-13 0.40510620598523417 1101 14-15 0.5307645680925077 1101 16-17 0.6048738500872446 1101 18-19 0.5267581340206036 1101 20-21 0.5757076691676986 1101 22-23 0.6800254308881719 1101 24-25 0.6673511445447105 1101 26-27 0.6814970848862743 1101 28-29 0.6536307018291936 1101 30-31 0.7313833547005899 1101 32-33 0.6404163173668778 1101 34-35 0.7203560966208542 1101 36-37 0.9130453810158912 1101 38-39 0.9104921600587872 1101 40-41 1.0172902171866198 1101 42-43 1.0985771111896696 1101 44-45 1.103832805917996 1101 46-47 1.1522655948745282 1101 48-49 1.0486137205152914 1101 50-51 1.112337657603561 1101 52-53 1.0202244413163584 1101 54-55 1.0220851564065825 1101 56-57 1.130473816495055 1101 58-59 1.4706590098918682 1101 60-61 1.3266755074073515 1101 62-63 1.3750329559346 1101 64-65 1.3406996495046322 1101 66-67 1.4132502759872487 1101 68-69 1.6577336652309178 1101 70-71 1.9945574435512299 1101 72-73 2.138194588379143 1101 74-75 2.3079132298244573 1101 76-77 2.2937198752360146 1101 78-79 2.3213119015815167 1101 80-81 2.3326360007700444 1101 82-83 2.49378082625471 1101 84-85 2.679463477020075 1101 86-87 2.8145396091898043 1101 88-89 3.1079087514683543 1101 90-91 3.2575893907313045 1101 92-93 3.7256883388199995 1101 94-95 3.9841165122868 1101 96-97 4.236655040166841 1101 98-99 4.372787220487151 1101 100-101 4.399119385075121 1102 1 0.487197495819931 1102 2 0.47145179671187876 1102 3 0.3795300238493482 1102 4 0.3169049587773216 1102 5 0.3449785533890761 1102 6 0.45251081358881606 1102 7 0.30602442403698404 1102 8 0.4193734729945362 1102 9 0.5192621245697211 1102 10-11 0.5088990818279555 1102 12-13 0.536286256890051 1102 14-15 0.7134793930091661 1102 16-17 0.8159444831762244 1102 18-19 0.6835765614812388 1102 20-21 0.721168408665136 1102 22-23 0.8012994925292034 1102 24-25 0.6838515016413993 1102 26-27 0.7650015491246833 1102 28-29 0.6768766895247254 1102 30-31 0.7712515574128318 1102 32-33 0.8230058405527316 1102 34-35 0.9022788450573671 1102 36-37 1.1405978276050348 1102 38-39 1.0823573915570464 1102 40-41 1.2849090180623008 1102 42-43 1.5620200000912803 1102 44-45 1.3667727924818394 1102 46-47 1.4892920179101239 1102 48-49 1.5822075648889609 1102 50-51 1.619238789504692 1102 52-53 1.5806853079698797 1102 54-55 1.6486208311074009 1102 56-57 1.7637972422496802 1102 58-59 1.713779070672473 1102 60-61 1.8538530024747573 1102 62-63 1.9527547320508774 1102 64-65 1.8972554996384652 1102 66-67 1.8655368074022185 1102 68-69 2.114431453350729 1102 70-71 2.2154508846281615 1102 72-73 2.479687369092108 1102 74-75 2.6047021676684423 1102 76-77 2.499787710891006 1102 78-79 2.82625853840889 1102 80-81 2.8055469851489185 1102 82-83 3.2049701383614497 1102 84-85 3.3003883892293935 1102 86-87 3.470633776751864 1102 88-89 3.7244828061158017 1102 90-91 3.740545176439383 1102 92-93 3.925723677616805 1102 94-95 4.048040528045679 1102 96-97 4.269333625597721 1102 98-99 4.381153031605258 1102 100-101 4.454781963673483 1103 1 0.7709242951981885 1103 2 0.47533645750483444 1103 3 0.6234102221729856 1103 4 0.532598788382245 1103 5 0.6215497769619134 1103 6 0.6347545606630547 1103 7 0.6337543583936878 1103 8 0.6346026182941671 1103 9 0.7157104614874044 1103 10-11 0.7810826793157233 1103 12-13 0.8001745989077023 1103 14-15 0.9298813427457588 1103 16-17 0.97148247635252 1103 18-19 1.038180217331103 1103 20-21 0.9901945278605666 1103 22-23 1.0454642201467053 1103 24-25 1.0464696336417418 1103 26-27 1.1472611085902145 1103 28-29 1.2785003658619516 1103 30-31 1.2513279443876755 1103 32-33 1.277932712930486 1103 34-35 1.2826168552335488 1103 36-37 1.4190932566989574 1103 38-39 1.4131466798806898 1103 40-41 1.4322447238793146 1103 42-43 1.4761698090613393 1103 44-45 1.561103800206162 1103 46-47 1.6063859003896752 1103 48-49 1.7289848370452106 1103 50-51 1.5922785061066378 1103 52-53 1.7127783060805157 1103 54-55 1.681390762064126 1103 56-57 1.896510079848916 1103 58-59 2.0129731074634734 1103 60-61 1.7593061524843492 1103 62-63 1.9086480040346316 1103 64-65 1.9902440790919833 1103 66-67 2.141764439587252 1103 68-69 2.429168195785625 1103 70-71 2.502897033435886 1103 72-73 2.7678682003488895 1103 74-75 2.953120084795259 1103 76-77 3.0297489111577534 1103 78-79 3.2073012312403755 1103 80-81 3.4149806077927636 1103 82-83 3.5291311910042147 1103 84-85 3.6775737648299334 1103 86-87 3.6801253052476035 1103 88-89 3.9153894623825423 1103 90-91 3.822913203909195 1103 92-93 4.256836244967904 1103 94-95 4.655794474737295 1103 96-97 5.067162515524103 1103 98-99 5.292085675193331 1103 100-101 4.908678843132435 1104 1 0.32476132568335814 1104 2 0.32532288843828994 1104 3 0.42164925886977045 1104 4 0.3573829397125152 1104 5 0.43071241478844513 1104 6 0.33965651159329013 1104 7 0.2386050484058444 1104 8 0.25082921165437 1104 9 0.3460046589525021 1104 10-11 0.3562382210230126 1104 12-13 0.2773936438464375 1104 14-15 0.5425437946258285 1104 16-17 0.5338328397019723 1104 18-19 0.6307333125859529 1104 20-21 0.6597163941600286 1104 22-23 0.5078175988442766 1104 24-25 0.5588999037164157 1104 26-27 0.6581314385698107 1104 28-29 0.6546157933880536 1104 30-31 0.577306173574236 1104 32-33 0.5901177346912405 1104 34-35 0.6233974283507138 1104 36-37 0.83626264890529 1104 38-39 0.7218580206049765 1104 40-41 0.8162593583688746 1104 42-43 0.8689088507837113 1104 44-45 0.7893797246616359 1104 46-47 0.8803855586502252 1104 48-49 1.0661811663614316 1104 50-51 1.0094157345996706 1104 52-53 0.9092754766788644 1104 54-55 1.1626865576637258 1104 56-57 1.2542751038209303 1104 58-59 1.2314913653963515 1104 60-61 1.1729345208794726 1104 62-63 1.3936255396911221 1104 64-65 1.4222922040480093 1104 66-67 1.4894672468763552 1104 68-69 1.5687858496437634 1104 70-71 1.8353180564429934 1104 72-73 2.119328131397932 1104 74-75 2.083529870605034 1104 76-77 2.1305419477138265 1104 78-79 2.3908585379314324 1104 80-81 2.498155970388371 1104 82-83 2.6614878865847196 1104 84-85 2.8223235411916114 1104 86-87 2.8954181458060475 1104 88-89 3.2445790373189674 1104 90-91 3.406689625897112 1104 92-93 3.694541163000693 1104 94-95 3.7773914031026727 1104 96-97 4.100544429466069 1104 98-99 4.455910543774408 1104 100-101 4.209242713231237 1105 1 0.3584646145541406 1105 2 0.20794283817006587 1105 3 0.11041500788235936 1105 4 0.08038895796556034 1105 5 0.1638706846612763 1105 6 0.29700890898020305 1105 7 0.2782054749890577 1105 8 0.3154865785212877 1105 9 0.3847487036687127 1105 10-11 0.30430761046239496 1105 12-13 0.2711599555630073 1105 14-15 0.32388721018540423 1105 16-17 0.4670859653886055 1105 18-19 0.393678451627423 1105 20-21 0.3708579329678443 1105 22-23 0.3080726913152958 1105 24-25 0.3535011609578831 1105 26-27 0.36458913662821146 1105 28-29 0.38188978735372103 1105 30-31 0.3486516791771592 1105 32-33 0.42077652707491353 1105 34-35 0.3650310919823525 1105 36-37 0.4008390935807711 1105 38-39 0.45167114929269303 1105 40-41 0.6225144829944043 1105 42-43 0.5637073223725153 1105 44-45 0.7704573295791377 1105 46-47 0.777156173712477 1105 48-49 0.8032542482107488 1105 50-51 0.8540086852585631 1105 52-53 0.8020975175258158 1105 54-55 0.8691662279727908 1105 56-57 1.0888867576883143 1105 58-59 1.1743613700587545 1105 60-61 1.3404188053611747 1105 62-63 1.244681547493954 1105 64-65 1.3558475821582867 1105 66-67 1.6028178007378422 1105 68-69 1.5940544738057376 1105 70-71 1.766224974486498 1105 72-73 1.965543813153996 1105 74-75 2.0262171304510126 1105 76-77 2.0882475088368757 1105 78-79 2.202229445599748 1105 80-81 2.31705371367746 1105 82-83 2.54415713990333 1105 84-85 2.6684309694200614 1105 86-87 2.768092704619331 1105 88-89 2.9028982137347015 1105 90-91 3.131002189201311 1105 92-93 3.3602360488253744 1105 94-95 3.7701979026015984 1105 96-97 3.76829559640694 1105 98-99 3.896146021992408 1105 100-101 4.040571927214163 1106 1 0.0618775504613609 1106 2 0.043183112769607845 1106 3 0.004518158736082967 1106 4 0.05145039081188685 1106 5 0.2126257942859482 1106 6 0.005870807315780269 1106 7 0.06826199868402227 1106 8 0.12831295885338534 1106 9 0.01849863032482091 1106 10-11 0.024828254324006593 1106 12-13 0.12629500612140276 1106 14-15 0.2446464661849319 1106 16-17 0.13301332559514378 1106 18-19 0.10206116984995361 1106 20-21 -0.004680411220384428 1106 22-23 -0.07695928662285922 1106 24-25 0.01805356761555288 1106 26-27 0.05661714421685815 1106 28-29 0.11510381461809516 1106 30-31 0.18705258668561697 1106 32-33 0.009884469729762202 1106 34-35 0.12411106616529821 1106 36-37 0.16756052368893393 1106 38-39 -0.10719759447307808 1106 40-41 0.4016730818326337 1106 42-43 0.051156715085070914 1106 44-45 0.21042877435606755 1106 46-47 0.3304625164924957 1106 48-49 0.45473188321240343 1106 50-51 0.25182988262209705 1106 52-53 0.4201420714971462 1106 54-55 0.03946488431262907 1106 56-57 0.4654773422880538 1106 58-59 0.4747486258235867 1106 60-61 0.5571529484111082 1106 62-63 0.48599665242461754 1106 64-65 0.522238475780302 1106 66-67 0.6746498334394424 1106 68-69 0.6136050224180671 1106 70-71 0.8112405233922075 1106 72-73 0.9581527043898888 1106 74-75 1.1022424781010756 1106 76-77 1.2698098315891286 1106 78-79 1.7662107477961548 1106 80-81 1.7895878919324595 1106 82-83 1.8090870231398526 1106 84-85 1.829102995080646 1106 86-87 1.6560916875839915 1106 88-89 1.970828351230061 1106 90-91 2.099904377783133 1106 92-93 2.148295662743113 1106 94-95 2.283714693463928 1106 96-97 2.5017247657628836 1106 98-99 2.6959778199935407 1106 100-101 2.960650551869449 1107 1 0.19962505816287646 1107 2 0.07595590972227484 1107 3 0.08073014297055181 1107 4 0.15073358079512644 1107 5 0.16581000858411699 1107 6 0.20543553600672482 1107 7 0.19962912698523638 1107 8 0.18138703517733745 1107 9 0.21375312884962483 1107 10-11 0.1247610955700793 1107 12-13 0.09393115694610543 1107 14-15 0.20304099279611876 1107 16-17 0.30327863344348316 1107 18-19 0.2865109107636741 1107 20-21 0.30079404959388256 1107 22-23 0.3501301883715726 1107 24-25 0.347564430340519 1107 26-27 0.3736955974731373 1107 28-29 0.46805155387140474 1107 30-31 0.38186558042553287 1107 32-33 0.5648054614162206 1107 34-35 0.6428591380559325 1107 36-37 0.5148199184700388 1107 38-39 0.6017085138964973 1107 40-41 0.44209727184803427 1107 42-43 0.4189542216153299 1107 44-45 0.4498143958316305 1107 46-47 0.5866047731457442 1107 48-49 0.7530268290546189 1107 50-51 0.5070244127272048 1107 52-53 0.6228802150886352 1107 54-55 0.8068896329586366 1107 56-57 0.8999906090701018 1107 58-59 0.9995707633181823 1107 60-61 0.9371786882988786 1107 62-63 0.9425135925899966 1107 64-65 1.1565263051371097 1107 66-67 1.3024716227328703 1107 68-69 1.4917449373489937 1107 70-71 1.4839821489953557 1107 72-73 1.6971440684451267 1107 74-75 1.794541237083532 1107 76-77 1.96020082933833 1107 78-79 2.1586233371939265 1107 80-81 2.3212336010181325 1107 82-83 2.250700893854386 1107 84-85 2.575885409849775 1107 86-87 2.4995840976323542 1107 88-89 2.680046728946138 1107 90-91 2.963032137436187 1107 92-93 3.3288322101677537 1107 94-95 3.69231168892858 1107 96-97 3.6512792509075602 1107 98-99 3.8455248607005217 1107 100-101 3.728505886791723 1108 1 0.05874990497979837 1108 2 0.15574470046588473 1108 3 0.046521764560658596 1108 4 0.11085617359106692 1108 5 0.030411222929231485 1108 6 0.0844427165963495 1108 7 0.04083222902466588 1108 8 0.13453637121024542 1108 9 0.15771958802898922 1108 10-11 0.10133248178140519 1108 12-13 0.14398409053241323 1108 14-15 0.2998407308694553 1108 16-17 0.2983242659786072 1108 18-19 0.3015583733877776 1108 20-21 0.3412210626338492 1108 22-23 0.22907887561090234 1108 24-25 0.24275612250874445 1108 26-27 0.306474576005499 1108 28-29 0.37477454403602906 1108 30-31 0.3430487754211313 1108 32-33 0.3596007501339855 1108 34-35 0.3933834546875872 1108 36-37 0.2893031499901966 1108 38-39 0.3704572735672045 1108 40-41 0.19986202278991527 1108 42-43 0.4084786996345713 1108 44-45 0.37310972972247924 1108 46-47 0.5219298533183121 1108 48-49 0.5898006524502577 1108 50-51 0.532187170957009 1108 52-53 0.4998943747264448 1108 54-55 0.6147107923393094 1108 56-57 0.7789653577031359 1108 58-59 0.6457242174373903 1108 60-61 0.7810966153717587 1108 62-63 0.84437063098094 1108 64-65 1.0652064094444675 1108 66-67 1.0479695652340943 1108 68-69 1.1682653204476168 1108 70-71 1.3408672603334892 1108 72-73 1.5608956813558272 1108 74-75 1.6841687251744375 1108 76-77 1.7631498594297774 1108 78-79 2.03145049262562 1108 80-81 2.163646144772315 1108 82-83 2.0518682898539815 1108 84-85 2.3458445764720395 1108 86-87 2.4474993405747334 1108 88-89 2.668000187544042 1108 90-91 2.8311083973211737 1108 92-93 2.9115669036355705 1108 94-95 3.1331316691759703 1108 96-97 3.524376286549508 1108 98-99 3.6882516855579155 1108 100-101 3.4297291893069755 1109 1 0.19886623577743023 1109 2 0.1068316408695189 1109 3 0.1270684229232799 1109 4 0.11562729330279353 1109 5 0.1520169438242931 1109 6 0.10349865028261007 1109 7 0.1508386053462658 1109 8 0.09033919458613582 1109 9 0.19725500627120596 1109 10-11 0.11975189545638898 1109 12-13 0.2529051946740566 1109 14-15 0.3265730884772964 1109 16-17 0.3677147711908759 1109 18-19 0.28863927852886917 1109 20-21 0.3366440129430259 1109 22-23 0.41311768220076317 1109 24-25 0.28047484516840626 1109 26-27 0.2733836911796601 1109 28-29 0.343565600874264 1109 30-31 0.34446917520167375 1109 32-33 0.2022798432134536 1109 34-35 0.1807546307482042 1109 36-37 0.39503294224074637 1109 38-39 0.2762595408685371 1109 40-41 0.4675320333182569 1109 42-43 0.5370681609095413 1109 44-45 0.8265388561664295 1109 46-47 0.8838800809381624 1109 48-49 0.9172494194476073 1109 50-51 0.8869412377156181 1109 52-53 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-1.366326470078036 2316 52-53 -1.4530793851534085 2316 54-55 -1.2826830129904039 2316 56-57 -1.2612027678671183 2316 58-59 -1.1315126146596697 2316 60-61 -1.292035593836502 2316 62-63 -1.3936866255579048 2316 64-65 -1.4361412659825703 2316 66-67 -1.6197852444936842 2316 68-69 -1.739349232397828 2316 70-71 -1.913073047048691 2316 72-73 -1.8637301804670265 2316 74-75 -1.9270908638040893 2316 76-77 -2.094910422237078 2316 78-79 -2.7492554249901744 2316 80-81 -2.770061873761893 2316 82-83 -2.6371003603033323 2316 84-85 -2.5183276967536337 2316 86-87 -2.527155400627475 2316 88-89 -3.017476367366548 2316 90-91 -2.852006578034974 2316 92-93 -3.2314244026813874 2316 94-95 -3.323939558869707 2316 96-97 -3.5310717745032036 2316 98-99 -3.5968470930336256 2316 100-101 -3.394596399907039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7840.0 3 2499.0 4 2601.0 5 2887.0 6 3364.0 7 3946.0 8 4882.0 9 6041.0 10 7276.0 11 9322.0 12 11130.0 13 13666.0 14 15645.0 15 18675.0 16 22334.0 17 27190.0 18 26259.0 19 22484.0 20 22695.0 21 24024.0 22 26475.0 23 29277.0 24 32375.0 25 36318.0 26 41271.0 27 46707.0 28 53369.0 29 61724.0 30 72622.0 31 86268.0 32 104155.0 33 129818.0 34 166459.0 35 221103.0 36 281893.0 37 308715.0 38 160519.0 39 9555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39061140505307 18.68176061112591 14.903622101932857 23.024005881888165 2 15.265695293849934 19.571197651141567 41.12075825311192 24.042348801896576 3 19.1012326552575 23.96989345195773 29.886600993531165 27.042272899253604 4 12.559618441971383 16.56674108468104 37.33374924490375 33.53989122844383 5 13.450258495667859 37.355298748785756 34.550423976256766 14.644018779289617 6 32.11175864807479 36.284562252705946 17.218995011111293 14.384684088107969 7 28.396372829959677 33.07691292888421 20.38332549638951 18.143388744766604 8 26.216262564968474 35.22478431786402 19.428352825445405 19.130600291722104 9 26.275235564919992 16.86130798779336 20.29554510327918 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22.2006816039036 26.69276042042135 29.384897945529172 21.721660030145873 36-37 22.306534725490835 26.22425099919386 29.35858420955674 22.110630065758567 38-39 21.785734978851615 26.623719826629866 29.343733468622712 22.24681172589581 40-41 22.37053150632793 26.3593373376212 29.36019795646597 21.909933199584906 42-43 21.985389817622377 26.384271914449226 29.19822526040992 22.432113007518478 44-45 22.059359305728098 26.688607982478345 29.263183924355452 21.988848787438105 46-47 22.32207775934601 26.975645177967582 28.529126839012463 22.173150223673943 48-49 22.18175248963833 26.946176328645215 28.530933790140935 22.341137391575515 50-51 22.358358970349297 27.197461034601083 28.35856292033775 22.085617074711873 52-53 22.576567079888445 26.985341530884714 28.29693091099552 22.14116047823132 54-55 22.159526737142368 27.179148286061295 28.43578467472599 22.22554030207035 56-57 22.233277934402548 27.26046595862484 28.241231924008716 22.2650241829639 58-59 22.2122132518297 26.965861874795554 28.240345288283073 22.581579585091674 60-61 21.993730248055805 27.167276520271184 28.464560781761904 22.374432449911108 62-63 22.092200068109335 27.152073637212755 28.584112437866715 22.17161385681119 64-65 21.919915555311672 27.14066790341144 28.45491137382059 22.484505167456295 66-67 21.770800339582962 27.160726123514472 28.44073252896287 22.627741007939694 68-69 22.206893693223538 26.89462109933028 28.460847174147215 22.437638033298963 70-71 22.12098424137027 26.900151225002595 28.145702616892027 22.833161916735104 72-73 22.081750802324677 26.998546194989803 28.160365549649217 22.759337453036306 74-75 22.078151770101677 27.284329021660415 28.016791385289284 22.62072782294862 76-77 22.19311507684192 26.970337087118914 28.086746829063813 22.749801006975353 78-79 22.254564705887894 27.055468851008936 28.047998443792725 22.641967999310445 80-81 22.33257545462125 27.151393455865698 27.76271283839002 22.75331825112304 82-83 22.369973220654877 27.157543610343687 27.861375734394866 22.611107434606563 84-85 22.417405610533308 27.222713242208503 27.732172971857842 22.627708175400347 86-87 22.380438921433594 27.121343312366943 27.700460387831594 22.797757378367873 88-89 22.39510021726145 27.19258823773264 27.486494176526342 22.925817368479564 90-91 22.399480857198043 27.147965120387425 27.467553574877634 22.985000447536898 92-93 22.5262661087535 26.767773708385256 27.65419526474066 23.051764918120583 94-95 22.374312525630497 26.685158703556837 27.718764007706163 23.221764763106503 96-97 22.598820154878815 26.356579287655435 27.75243414305775 23.292166414407998 98-99 22.347621343433932 26.531382269765963 27.41792458984964 23.70307179695046 100-101 23.17773689078472 25.557528109283666 27.521628784359265 23.74310621557235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 767.0 1 517.5 2 364.5 3 484.5 4 407.5 5 379.5 6 721.5 7 1665.5 8 2860.0 9 3314.5 10 2755.5 11 2118.5 12 2051.0 13 2228.5 14 2389.5 15 2523.0 16 2720.0 17 2940.5 18 3222.0 19 3642.0 20 4211.0 21 5004.5 22 6049.5 23 7360.5 24 9076.5 25 11429.5 26 14248.0 27 17554.0 28 20471.5 29 23087.5 30 26283.5 31 30633.5 32 36238.5 33 39441.5 34 41152.0 35 43879.5 36 47700.0 37 51807.5 38 55263.0 39 58053.5 40 60793.5 41 64327.0 42 66314.5 43 68364.5 44 71294.5 45 74840.5 46 76773.0 47 77993.5 48 79500.0 49 77725.0 50 75110.0 51 72867.0 52 71339.5 53 71094.0 54 70910.0 55 67515.5 56 61947.5 57 56426.5 58 52002.0 59 46419.0 60 37811.0 61 31136.0 62 26176.0 63 20237.0 64 14922.0 65 11315.0 66 8335.5 67 6242.5 68 4752.0 69 3473.5 70 2639.0 71 1878.5 72 1268.0 73 944.5 74 670.0 75 458.5 76 327.0 77 214.5 78 137.0 79 84.5 80 53.0 81 34.0 82 19.5 83 12.0 84 8.5 85 6.5 86 5.5 87 5.5 88 5.0 89 3.5 90 3.5 91 3.0 92 4.0 93 3.5 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4603973941582842 2 0.03861762103209831 3 0.04963777142418489 4 0.05354662818718997 5 0.07930740709518726 6 0.07855389253846339 7 0.04506958942404644 8 0.0383821477331221 9 0.04389222292916539 10-11 0.04707111246534421 12-13 0.04222036250643431 14-15 0.03689866594957198 16-17 0.03715768657844581 18-19 0.045564083351896476 20-21 0.05404112211504 22-23 0.04582310398077031 24-25 0.06433130528030034 26-27 0.05571298253777109 28-29 0.04330353968172487 30-31 0.045163778743636925 32-33 0.04417479088793685 34-35 0.04879006754787055 36-37 0.043421276331212975 38-39 0.024771791052297206 40-41 0.023170572619258985 42-43 0.022605436701716084 44-45 0.013916471969493962 46-47 0.025360474299737732 48-49 0.025172095660556764 50-51 0.014764175845808315 52-53 0.014599344536524969 54-55 0.015753163701508394 56-57 0.014010661289084446 58-59 0.013539714691132028 60-61 0.01565897438191791 62-63 0.015352859093248841 64-65 0.015847353021098878 66-67 0.00897153269099357 68-69 0.011962043587991427 70-71 0.018249180670656215 72-73 0.015706069041713153 74-75 0.012739105474612917 76-77 0.009089269340481675 78-79 0.012833294794203403 80-81 0.016577320247925128 82-83 0.013728093330312998 84-85 0.011844306938503323 86-87 0.0030376055567930987 88-89 0.028021322578168893 90-91 0.030752812846292922 92-93 0.016600867577822746 94-95 0.03143568541332392 96-97 0.02646719880492591 98-99 0.02225222675325177 100-101 0.14957263950968808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2123383.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.51286917662522 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74473346959975 44.13809816032062 2 13.279861764590573 15.2752590470113 3 4.410879397848377 7.610469892869753 4 1.9546531808406507 4.4967085070145085 5 1.0263584566927875 2.9514409824047623 6 0.6105933827223039 2.1070186404372553 7 0.38443059529652934 1.5476794574347015 8 0.2690953346841802 1.2381155823785148 9 0.20233667935337546 1.0473266672355104 >10 1.0215161281757759 10.455626530634845 >50 0.060342592943591566 2.371726333500577 >100 0.030267407754424956 3.1469765638839027 >500 0.0034521266483529835 1.4256240093210961 >1k 0.0012329023744117478 1.1107935861471747 >5k 2.465804748823496E-4 1.077136039405461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9799 0.4614805713335748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7972 0.3754386278876679 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5015 0.23617971887313782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.709465979524184E-5 0.0 0.0 0.0 0.0 8 4.709465979524184E-5 0.0 0.0 0.0 0.0 9 4.709465979524184E-5 0.0 0.0 0.0 0.0 10-11 4.709465979524184E-5 0.0 0.0 0.0 4.709465979524184E-5 12-13 4.709465979524184E-5 0.0 0.0 0.0 1.6483130928334643E-4 14-15 1.412839793857255E-4 0.0 0.0 0.0 2.1192596907858828E-4 16-17 1.6483130928334643E-4 0.0 0.0 2.354732989762092E-5 2.82567958771451E-4 18-19 2.3547329897620917E-4 0.0 0.0 4.709465979524184E-5 2.82567958771451E-4 20-21 2.82567958771451E-4 0.0 0.0 4.709465979524184E-5 2.82567958771451E-4 22-23 2.82567958771451E-4 0.0 0.0 1.412839793857255E-4 2.82567958771451E-4 24-25 5.180412577476602E-4 0.0 0.0 4.944939278500394E-4 2.82567958771451E-4 26-27 5.180412577476602E-4 0.0 0.0 0.0012951031443691505 2.82567958771451E-4 28-29 5.180412577476602E-4 0.0 0.0 0.004379803360957491 2.82567958771451E-4 30-31 5.65135917542902E-4 0.0 0.0 0.014175492598367794 2.82567958771451E-4 32-33 5.65135917542902E-4 0.0 0.0 0.03461457494950275 2.82567958771451E-4 34-35 5.65135917542902E-4 0.0 0.0 0.06327167543490742 2.82567958771451E-4 36-37 5.65135917542902E-4 0.0 0.0 0.1095186313538349 2.82567958771451E-4 38-39 5.65135917542902E-4 0.0 0.0 0.17672271088164498 2.82567958771451E-4 40-41 5.65135917542902E-4 0.0 0.0 0.25117936801792234 2.82567958771451E-4 42-43 5.886832474405229E-4 0.0 0.0 0.3261069717521521 2.82567958771451E-4 44-45 6.122305773381439E-4 0.0 0.0 0.41723513845594506 2.82567958771451E-4 46-47 7.535145567238694E-4 0.0 0.0 0.5225152504282082 2.82567958771451E-4 48-49 8.006092165191112E-4 0.0 0.0 0.6333289849264123 2.82567958771451E-4 50-51 8.006092165191112E-4 0.0 0.0 0.7512069183939025 2.82567958771451E-4 52-53 8.006092165191112E-4 0.0 0.0 0.8662827196035761 3.2966261856669286E-4 54-55 8.006092165191112E-4 0.0 0.0 0.9878811311948904 3.2966261856669286E-4 56-57 8.477038763143531E-4 0.0 0.0 1.1240317926629346 3.2966261856669286E-4 58-59 8.947985361095949E-4 0.0 0.0 1.2715558144715295 3.2966261856669286E-4 60-61 8.947985361095949E-4 0.0 0.0 1.4227767670740512 3.2966261856669286E-4 62-63 8.947985361095949E-4 0.0 0.0 1.5700182209238749 3.2966261856669286E-4 64-65 8.947985361095949E-4 0.0 0.0 1.7247948203409371 3.2966261856669286E-4 66-67 8.947985361095949E-4 0.0 0.0 1.9006933746761654 3.2966261856669286E-4 68-69 8.947985361095949E-4 0.0 0.0 2.0975725999501735 3.2966261856669286E-4 70-71 9.183458660072159E-4 0.0 0.0 2.293557026688073 3.2966261856669286E-4 72-73 9.654405258024577E-4 0.0 0.0 2.494674771343653 3.767572783619347E-4 74-75 9.889878557000787E-4 0.0 0.0 2.697699849720941 4.2385193815717656E-4 76-77 9.889878557000787E-4 0.0 0.0 2.9173964376657437 4.2385193815717656E-4 78-79 9.889878557000787E-4 0.0 0.0 3.158379811837996 4.2385193815717656E-4 80-81 0.0011067245051881833 0.0 0.0 3.4068747842475897 4.2385193815717656E-4 82-83 0.001130271835085804 0.0 0.0 3.670039743183401 4.4739926805479745E-4 84-85 0.001130271835085804 0.0 0.0 3.9272943223149097 4.944939278500394E-4 86-87 0.001130271835085804 0.0 0.0 4.195757430477686 5.415885876452811E-4 88-89 0.001130271835085804 0.0 0.0 4.481080426847159 5.65135917542902E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4885 0.0 52.752716 1 GTATCAA 8475 0.0 40.37338 1 ATCAACG 10550 0.0 32.257607 3 TATCAAC 10755 0.0 31.598555 2 TCAACGC 10740 0.0 31.554176 4 CAACGCA 10815 0.0 31.291405 5 AACGCAG 11060 0.0 30.678404 6 ACGCAGA 12475 0.0 27.03608 7 CGCAGAG 12540 0.0 26.782295 8 GCAGAGT 13365 0.0 24.452595 9 GTACATG 7365 0.0 24.039024 1 TACATGG 7280 0.0 23.308184 2 GAGTACT 8720 0.0 22.85398 12-13 GTATACG 125 2.2470171E-4 22.8146 4 TGGTATC 2145 0.0 22.601875 2 TAAGGTG 1305 0.0 22.581497 5 GTGGTAT 2480 0.0 22.321426 1 CAGAGTA 12780 0.0 22.263647 10-11 AGAGTAC 12000 0.0 21.612835 10-11 GTACTTT 9210 0.0 21.511915 14-15 >>END_MODULE