FastQCFastQC Report
Thu 26 May 2016
SRR938082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123383
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94820.4465515641784831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.3895670258262405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.2418781727083621No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22310.10506818600318453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.051.7682341
GTATCAA88650.038.370091
ATCAACG106550.031.562163
TATCAAC108200.031.1298872
TCAACGC108800.030.9953064
CAACGCA109600.030.7257275
AACGCAG113650.029.7143786
ACGCAGA129800.025.7611037
CGCAGAG130050.025.565498
GAGTACT90400.023.22310612-13
GCAGAGT144000.022.8579469
GTACATG77500.022.3130131
GTACTTT93450.022.160214-15
TACATGG73900.022.049982
CAGAGTA137100.021.4793310-11
ACATGGG74800.020.955563
GTGGTAT25000.020.9031261
TAAGGTG13800.020.6508855
AGAGTAC126800.020.45219210-11
AGTACTT94050.019.7209912-13
Produced by FastQC (version 0.11.5)