##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2123383 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47144721418604 33.0 31.0 34.0 31.0 34.0 2 32.70168452888622 34.0 31.0 34.0 31.0 34.0 3 32.668016556598594 34.0 31.0 34.0 31.0 34.0 4 36.20244016270263 37.0 35.0 37.0 35.0 37.0 5 36.16492973712232 37.0 35.0 37.0 35.0 37.0 6 36.222380512606534 37.0 37.0 37.0 35.0 37.0 7 36.181904536298916 37.0 37.0 37.0 35.0 37.0 8 36.17408211330692 37.0 37.0 37.0 35.0 37.0 9 37.87282087122295 39.0 38.0 39.0 35.0 39.0 10-11 37.834803000683344 39.0 38.0 39.0 35.0 39.0 12-13 37.781973624164834 39.0 38.0 39.0 35.0 39.0 14-15 39.055272882941985 40.0 38.0 41.0 36.0 41.0 16-17 38.990511132471156 40.0 38.0 41.0 36.0 41.0 18-19 38.99270715645741 40.0 38.5 41.0 36.0 41.0 20-21 38.998533001347376 40.0 38.5 41.0 35.5 41.0 22-23 39.02735140104258 40.0 39.0 41.0 35.0 41.0 24-25 38.98172962673244 40.0 38.5 41.0 35.0 41.0 26-27 38.81142026662171 40.0 38.0 41.0 35.0 41.0 28-29 38.732025969879196 40.0 38.0 41.0 35.0 41.0 30-31 38.51319969124741 40.0 38.0 41.0 34.0 41.0 32-33 38.30969895680619 40.0 38.0 41.0 34.0 41.0 34-35 38.10696586531964 40.0 38.0 41.0 34.0 41.0 36-37 38.02540333044015 40.0 38.0 41.0 33.0 41.0 38-39 37.97209358839173 40.0 38.0 41.0 33.0 41.0 40-41 37.84674620640742 40.0 37.5 41.0 33.0 41.0 42-43 37.65400306962992 40.0 37.0 41.0 33.0 41.0 44-45 37.403026679595726 40.0 37.0 41.0 32.5 41.0 46-47 37.0414011508993 40.0 37.0 41.0 31.5 41.0 48-49 36.66671721493485 40.0 36.0 41.0 30.5 41.0 50-51 36.364530562785895 39.0 36.0 41.0 30.0 41.0 52-53 35.89992832192779 39.0 35.0 40.5 29.0 41.0 54-55 35.58445014394483 39.0 35.0 40.0 28.0 41.0 56-57 35.31557966697483 38.0 35.0 40.0 28.0 41.0 58-59 34.944710162980485 38.0 34.0 40.0 26.5 41.0 60-61 34.538627275437356 38.0 34.0 40.0 26.0 41.0 62-63 34.1080094829807 37.0 33.5 40.0 25.5 41.0 64-65 33.67568450910646 36.5 33.0 39.0 24.5 41.0 66-67 33.162805061545654 36.0 32.5 39.0 22.5 40.0 68-69 32.64253740375618 35.0 32.0 39.0 21.5 40.0 70-71 32.05595292982943 35.0 31.0 38.0 19.5 40.0 72-73 31.580712947216774 35.0 31.0 37.0 18.0 39.5 74-75 31.02589570510831 34.5 30.0 36.5 15.5 39.0 76-77 29.82504899021985 33.5 29.0 35.0 14.0 37.5 78-79 30.508824361879135 34.0 30.0 36.0 14.0 38.0 80-81 30.319590248202985 34.0 30.0 35.5 9.5 37.0 82-83 29.85444924443683 34.0 30.0 35.0 7.0 37.0 84-85 29.34130394752148 34.0 29.0 35.0 2.0 36.5 86-87 28.80470032961552 34.0 29.0 35.0 2.0 36.0 88-89 28.181928319102113 33.5 27.5 35.0 2.0 35.5 90-91 27.65424372334148 33.0 26.5 35.0 2.0 35.0 92-93 27.02800578134044 33.0 25.5 35.0 2.0 35.0 94-95 26.283338898352298 32.0 24.5 34.5 2.0 35.0 96-97 25.47593039032525 32.0 21.5 34.0 2.0 35.0 98-99 24.47657158411836 32.0 10.0 34.0 2.0 35.0 100-101 21.001718484135928 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0713243017952152 1101 2 0.004006834165018347 1101 3 0.17447523615278726 1101 4 -0.004843162818673363 1101 5 0.011235312686835641 1101 6 0.00850134928619184 1101 7 0.05399836345352327 1101 8 0.06753035158013176 1101 9 0.0898488026086639 1101 10-11 0.15113921561998467 1101 12-13 0.012213430493254407 1101 14-15 0.14417369840292338 1101 16-17 0.12889307056865817 1101 18-19 0.2058860946783625 1101 20-21 0.17171044867087204 1101 22-23 0.1050333443891418 1101 24-25 0.22328497892377897 1101 26-27 0.23240458620341542 1101 28-29 0.1858216048456569 1101 30-31 0.18742243485226595 1101 32-33 0.2740131780177961 1101 34-35 0.19823485309974131 1101 36-37 0.35194347325887776 1101 38-39 0.44636853762705897 1101 40-41 0.3258204798281241 1101 42-43 0.33757344719158766 1101 44-45 0.43760164095341025 1101 46-47 0.3231079860197852 1101 48-49 0.45396522137139783 1101 50-51 0.5358587437370872 1101 52-53 0.47793442554505816 1101 54-55 0.4685502178550607 1101 56-57 0.5761121880494215 1101 58-59 0.6227197897015699 1101 60-61 0.6861720549817889 1101 62-63 0.7016538227712985 1101 64-65 0.7855491497796052 1101 66-67 0.7146803569380538 1101 68-69 0.8059988650953116 1101 70-71 0.8319828735556243 1101 72-73 0.9575477218938104 1101 74-75 0.9163308245197648 1101 76-77 0.9986711466415059 1101 78-79 1.0527887192910406 1101 80-81 1.2011791459737076 1101 82-83 1.14662627741318 1101 84-85 1.4423778656431487 1101 86-87 1.4751065978810907 1101 88-89 1.4887841505910266 1101 90-91 1.633954968244499 1101 92-93 1.8923270582978482 1101 94-95 2.076934683772926 1101 96-97 2.225433423120851 1101 98-99 2.51677968431407 1101 100-101 2.8659945900203176 1102 1 0.13489651903716293 1102 2 0.10227570995682811 1102 3 0.23891529371211107 1102 4 0.10783779619256251 1102 5 0.017885576217828714 1102 6 -0.007392632204123117 1102 7 0.14548664072161444 1102 8 0.10531054532363271 1102 9 0.12148311864664407 1102 10-11 0.23109417057494142 1102 12-13 0.1761631953604521 1102 14-15 0.2576374620960422 1102 16-17 0.24451239490357324 1102 18-19 0.28302639658930673 1102 20-21 0.2750597400935604 1102 22-23 0.2832161143150387 1102 24-25 0.1905380470942717 1102 26-27 0.2507187719487973 1102 28-29 0.12765140218004234 1102 30-31 0.21515358873020318 1102 32-33 0.15755568311993073 1102 34-35 0.14450239799113973 1102 36-37 0.23146334245765132 1102 38-39 0.28384715950934947 1102 40-41 0.10994970432432183 1102 42-43 0.07020021651560882 1102 44-45 0.08895103198170062 1102 46-47 0.21665926489216503 1102 48-49 0.2146047423320212 1102 50-51 0.33930112401433377 1102 52-53 0.30418225064609317 1102 54-55 0.25485434131515206 1102 56-57 0.2507271700038558 1102 58-59 0.36228027467490875 1102 60-61 0.421032532961533 1102 62-63 0.3921355006382967 1102 64-65 0.34427539543246155 1102 66-67 0.285276358726712 1102 68-69 0.32970473398466993 1102 70-71 0.539553005437682 1102 72-73 0.5339526377482713 1102 74-75 0.5929771250192815 1102 76-77 0.7427432886494287 1102 78-79 0.868208408563941 1102 80-81 0.9037327051786406 1102 82-83 1.0180531912712887 1102 84-85 0.9158769469403936 1102 86-87 0.9442299202154238 1102 88-89 1.122459639771101 1102 90-91 1.5057116932122305 1102 92-93 1.6356834174798962 1102 94-95 1.559062100533371 1102 96-97 1.8693561037407775 1102 98-99 2.204341540453907 1102 100-101 2.5406347382843144 1103 1 0.29266887185223567 1103 2 0.34594372223924097 1103 3 0.4412570276126928 1103 4 0.22205263596553948 1103 5 0.13361728056236188 1103 6 0.21562177207184874 1103 7 0.23992724144248712 1103 8 0.2417446073394558 1103 9 0.25218878254520405 1103 10-11 0.3488644546542403 1103 12-13 0.34374001201982907 1103 14-15 0.4894500871342444 1103 16-17 0.40617503347294104 1103 18-19 0.4745566423188734 1103 20-21 0.5170427639602693 1103 22-23 0.4542263448696957 1103 24-25 0.4764812374848404 1103 26-27 0.5581540020815936 1103 28-29 0.5664013545241176 1103 30-31 0.5514597141255848 1103 32-33 0.5390419666517374 1103 34-35 0.5926483794875068 1103 36-37 0.5053758343092696 1103 38-39 0.5316248310378597 1103 40-41 0.6280772359475435 1103 42-43 0.6823777265452264 1103 44-45 0.7741142902342943 1103 46-47 0.5993310090239277 1103 48-49 0.530616632250279 1103 50-51 0.6039118048159011 1103 52-53 0.6170518184178135 1103 54-55 0.6393123231665285 1103 56-57 0.6832757587323073 1103 58-59 0.8544765783253112 1103 60-61 0.7309088400619217 1103 62-63 0.7235093161933079 1103 64-65 0.8155562178768037 1103 66-67 0.6604717480084119 1103 68-69 0.7482735750738883 1103 70-71 1.0374184888624782 1103 72-73 0.8248943875237416 1103 74-75 0.7111297502199818 1103 76-77 0.47744948754430894 1103 78-79 0.9061954911527472 1103 80-81 1.0538824541491145 1103 82-83 1.054880750581816 1103 84-85 1.310024080928848 1103 86-87 1.2715093889190747 1103 88-89 1.470842400889623 1103 90-91 1.5092122941456019 1103 92-93 1.8815039149639752 1103 94-95 1.6519264620908842 1103 96-97 2.048414872711742 1103 98-99 2.7570514905730015 1103 100-101 2.883409100460579 1104 1 0.11623683277332475 1104 2 0.15294737081047316 1104 3 0.23075557522001588 1104 4 0.09352844784801562 1104 5 0.0146999915613506 1104 6 0.036138200693535794 1104 7 0.05458384354719925 1104 8 0.01702277320369916 1104 9 0.04672095128408671 1104 10-11 0.1543182142907895 1104 12-13 0.0940913000760375 1104 14-15 0.2064435840334653 1104 16-17 0.12298057367255666 1104 18-19 0.14216979325714618 1104 20-21 0.1805449902922973 1104 22-23 0.2278480794989619 1104 24-25 0.1963141871988867 1104 26-27 0.1730337399473285 1104 28-29 0.14046332997918398 1104 30-31 0.1896681952947432 1104 32-33 0.19314535288767587 1104 34-35 0.14484807594246973 1104 36-37 0.2146440093200752 1104 38-39 0.054258034041147596 1104 40-41 0.13653026184938 1104 42-43 0.15090754331257017 1104 44-45 0.11206144123287487 1104 46-47 0.07698627432430527 1104 48-49 0.17834054697623714 1104 50-51 0.18496737768178662 1104 52-53 0.2377732907117931 1104 54-55 0.20151895492215743 1104 56-57 0.29553113861591385 1104 58-59 0.24845291789372226 1104 60-61 0.42382779755556754 1104 62-63 0.30111131034353633 1104 64-65 0.37032800627977736 1104 66-67 0.22159486467223388 1104 68-69 0.42796118500845637 1104 70-71 0.530501280270748 1104 72-73 0.5029225508751978 1104 74-75 0.5469038403386861 1104 76-77 0.6433398695889139 1104 78-79 0.7009407444922466 1104 80-81 0.8260839598129444 1104 82-83 0.7825877793582876 1104 84-85 0.9459720561225851 1104 86-87 1.0020277272612397 1104 88-89 1.2493751324934834 1104 90-91 1.1112456438958311 1104 92-93 1.4517705722495577 1104 94-95 1.658489869836309 1104 96-97 1.9477605421692807 1104 98-99 2.208209886809847 1104 100-101 2.6351906686426254 1105 1 0.0822623741749382 1105 2 0.09380235931523373 1105 3 0.12244095402697042 1105 4 0.12861330896731715 1105 5 0.09967901074151797 1105 6 0.09267548117199453 1105 7 0.05068028565032279 1105 8 0.0740166477689499 1105 9 0.08221739017175622 1105 10-11 0.058287153031088224 1105 12-13 0.044818795936144795 1105 14-15 0.12788742885578586 1105 16-17 0.10010317347212805 1105 18-19 0.1658783876225982 1105 20-21 0.1338845044376029 1105 22-23 0.12262565065422137 1105 24-25 0.14978607075667583 1105 26-27 0.14058072572967717 1105 28-29 0.08617793723860245 1105 30-31 0.12723484892096337 1105 32-33 0.06398924808539874 1105 34-35 0.1223415244604169 1105 36-37 0.09486523237057298 1105 38-39 0.15124301252441796 1105 40-41 0.2083323615390853 1105 42-43 0.091595864257485 1105 44-45 0.14606001571612381 1105 46-47 0.12813660203239152 1105 48-49 0.34654991572954685 1105 50-51 0.5171411835435507 1105 52-53 0.4734256789882565 1105 54-55 0.4099230728434762 1105 56-57 0.3878692540398987 1105 58-59 0.4707820836448775 1105 60-61 0.537103324219693 1105 62-63 0.44361743309875123 1105 64-65 0.5409044831097702 1105 66-67 0.5810592776647994 1105 68-69 0.5901533725335781 1105 70-71 0.7007583556034191 1105 72-73 0.6280834574181 1105 74-75 0.6151191040860802 1105 76-77 0.6337964100848446 1105 78-79 0.7698698384316565 1105 80-81 0.8723827073946602 1105 82-83 1.0071650814874538 1105 84-85 1.0890821478165407 1105 86-87 1.277348066598531 1105 88-89 1.346381547318856 1105 90-91 1.311408024187255 1105 92-93 1.5577722121297306 1105 94-95 1.615128972939754 1105 96-97 1.7534138414902856 1105 98-99 1.899178219973475 1105 100-101 2.359142565490945 1106 1 0.004580432419771796 1106 2 0.0022105034213169006 1106 3 0.08078944509857422 1106 4 -0.013083184216590382 1106 5 -0.027767252631520023 1106 6 -0.05009672246977459 1106 7 -0.04305516781053598 1106 8 -0.11857072925779022 1106 9 -0.061571848811993846 1106 10-11 0.022029843089562462 1106 12-13 0.0036269087488349783 1106 14-15 -0.020230077842953165 1106 16-17 -0.13946536884241567 1106 18-19 -0.07619558214015854 1106 20-21 -0.03291320332120051 1106 22-23 -0.05262127379178594 1106 24-25 -0.07084214415071699 1106 26-27 0.048327796863475214 1106 28-29 -0.052078238317342596 1106 30-31 -0.07173246903948893 1106 32-33 -0.134620151668166 1106 34-35 -0.216632443346505 1106 36-37 -0.15822205664348843 1106 38-39 -0.10118711519174894 1106 40-41 -0.053284333487219726 1106 42-43 -0.023671400895359795 1106 44-45 0.11458277188190635 1106 46-47 -0.17683122444574195 1106 48-49 -0.10241251263791895 1106 50-51 -0.015667985421224273 1106 52-53 -0.10922408924503912 1106 54-55 -0.008000219221692362 1106 56-57 -0.03644571398216101 1106 58-59 -0.1039856606880889 1106 60-61 0.1416741157470085 1106 62-63 -0.002837611983302679 1106 64-65 -0.11023479205697129 1106 66-67 0.17961391397687265 1106 68-69 -0.17043455212757408 1106 70-71 0.035773708925404435 1106 72-73 0.21644427026404145 1106 74-75 0.32385778467830306 1106 76-77 0.2546847513393189 1106 78-79 0.5825573543228337 1106 80-81 0.52483630515718 1106 82-83 0.7689910510937459 1106 84-85 0.6547278674668355 1106 86-87 0.8297253867104075 1106 88-89 0.9751186632413322 1106 90-91 0.7833574071206222 1106 92-93 0.791545608832191 1106 94-95 1.1441359943952918 1106 96-97 1.178880797620856 1106 98-99 1.4198553308107726 1106 100-101 1.588294318898992 1107 1 0.10643383933194173 1107 2 0.07471813545884487 1107 3 0.16824511099062534 1107 4 0.06829027681961719 1107 5 0.046288425409848344 1107 6 -0.006351221391618367 1107 7 0.055101629119896245 1107 8 0.02654299991316833 1107 9 0.006264845238298733 1107 10-11 0.0767105283272258 1107 12-13 0.036496284873194895 1107 14-15 0.08902945272469509 1107 16-17 0.06097869682904644 1107 18-19 0.12408708812208147 1107 20-21 0.1348356860679516 1107 22-23 0.15770384366158652 1107 24-25 0.11792517210765396 1107 26-27 0.1180585543816619 1107 28-29 0.10940084638606606 1107 30-31 0.15087200024356662 1107 32-33 0.047443696593951756 1107 34-35 0.011205862672689193 1107 36-37 -0.06859410383209763 1107 38-39 -0.0347887857632756 1107 40-41 0.017635945915436935 1107 42-43 0.10377975466003875 1107 44-45 0.04308599522416756 1107 46-47 0.08296168519387237 1107 48-49 0.13219675761263971 1107 50-51 0.19701741581808818 1107 52-53 0.1420492073782853 1107 54-55 0.13847881982250243 1107 56-57 0.17666441724242787 1107 58-59 0.30990383368173724 1107 60-61 0.31731341427848747 1107 62-63 0.3651690336166027 1107 64-65 0.30452004375050024 1107 66-67 0.5659973029151928 1107 68-69 0.6165011743840338 1107 70-71 0.5915916064653217 1107 72-73 0.5310949434992835 1107 74-75 0.4069399923134007 1107 76-77 0.42114505819308334 1107 78-79 0.6245808080734179 1107 80-81 0.8069561752944239 1107 82-83 1.0277632786958115 1107 84-85 1.1226721187529378 1107 86-87 1.1998548881633617 1107 88-89 1.1568961488001115 1107 90-91 1.220662933593239 1107 92-93 1.4036627116237916 1107 94-95 1.5279081723677734 1107 96-97 1.6605113994609617 1107 98-99 1.9510106003411032 1107 100-101 2.386866495600959 1108 1 0.061421758428110707 1108 2 0.07746172454903899 1108 3 0.16955011114892216 1108 4 0.07413845356451532 1108 5 -0.0330467040361313 1108 6 0.038908346228623714 1108 7 -0.0328345324344923 1108 8 -0.027428022905802152 1108 9 -0.014630601083339911 1108 10-11 0.016527241441089302 1108 12-13 -0.01193427869382191 1108 14-15 0.04471195622464563 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sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 20.0 5 35.0 6 83.0 7 209.0 8 465.0 9 876.0 10 1531.0 11 2616.0 12 4107.0 13 5889.0 14 8379.0 15 11151.0 16 14637.0 17 20652.0 18 20454.0 19 15176.0 20 13618.0 21 13611.0 22 14394.0 23 15566.0 24 18117.0 25 20692.0 26 24245.0 27 28859.0 28 35268.0 29 43024.0 30 52070.0 31 63322.0 32 80322.0 33 104512.0 34 143476.0 35 211828.0 36 326584.0 37 462687.0 38 319983.0 39 24916.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67261252444802 19.12980371416744 15.300018884958577 22.897564876425967 2 15.479089325698222 19.884098053197715 40.56017076320641 24.076641857897656 3 19.311468337546955 23.94516576302989 30.04556175562999 26.697804143793164 4 12.814174362326533 16.973433431462905 37.022666188812856 33.18972601739771 5 13.410980271002915 37.41351020923324 34.66250850063013 14.513001019133718 6 32.17453674116892 36.44122274806076 17.03749821628808 14.346742294482242 7 28.411454739912678 33.03779864489826 20.347389048513623 18.203357566675443 8 26.277327918062632 35.47455987582051 19.21494901049177 19.033163195625082 9 26.048291806047235 16.833844859829806 20.286684032037556 36.831179302085395 10-11 24.91846737022949 26.70523876286096 27.006197186282456 21.370096680627093 12-13 25.899802343712842 23.941206084818425 27.596670030795202 22.562321540673537 14-15 22.55645825552903 25.448258745596057 26.160800948298068 25.83448205057684 16-17 21.780602411482622 28.61269695874389 27.141789102999574 22.46491152677391 18-19 21.715795030854064 26.95168511756946 29.643757155444874 21.688762696131597 20-21 22.298191141211923 26.086909427079334 30.090285172293456 21.52461425941528 22-23 22.042019739255707 26.084554694089572 29.933224481876326 21.940201084778394 24-25 21.924349312866948 26.1657572945412 30.077568410128986 21.83232498246287 26-27 21.526813924705035 26.533862008297543 29.889739003046518 22.049585063950907 28-29 21.608493636083637 26.828313187760255 29.74644646407752 21.816746712078583 30-31 21.784958668539463 26.526096923187414 29.59739943227374 22.091544975999376 32-33 21.524683001356134 26.454666256301767 29.68176861306632 22.338882129275774 34-35 22.18686941956926 26.662259472719356 29.47435380519669 21.676517302514693 36-37 22.216617531425715 26.217495442656357 29.52640598260709 22.039481043310836 38-39 21.754764041291892 26.601521007473146 29.41978911271424 22.223925838520728 40-41 22.27658211912684 26.371309656630576 29.409952524590903 21.94215569965168 42-43 21.92549225755944 26.392716067177457 29.301795500086197 22.3799961751769 44-45 21.967915048022395 26.70798369989111 29.35352509636507 21.970576155721428 46-47 22.31306738654153 26.962104842765992 28.639185958672503 22.085641812019976 48-49 22.08101381830889 26.964575557301725 28.581220917514234 22.373189706875152 50-51 22.273466444819423 27.236207504722415 28.429397805294666 22.060928245163495 52-53 22.60713210946871 27.00782195204539 28.34700569798289 22.03804024050301 54-55 22.189214098445735 27.121532008121 28.469757928739188 22.219495964694076 56-57 22.22943293791087 27.22033660437142 28.312061460414817 22.23816899730289 58-59 22.189708592373584 26.952438632126185 28.299298807610306 22.558553967889917 60-61 21.895979199230663 27.235924936763645 28.48341538007981 22.384680483925887 62-63 22.00625134514122 27.240045719495733 28.576851185113565 22.176851750249487 64-65 21.817260475382916 27.16471781115324 28.443596845222928 22.574424868240918 66-67 21.66622162129323 27.28295325516547 28.35711382659431 22.69371129694699 68-69 22.106881977526864 27.000708069377144 28.4625993610657 22.4298105920303 70-71 22.056948746410797 27.07059913355245 28.00660549698288 22.865846623053873 72-73 22.062411726947044 27.190478590061236 27.97728907126034 22.769820611731372 74-75 22.02998705367802 27.506789872575975 27.81462411632758 22.64859895741842 76-77 22.07901259452487 27.181342226060963 28.003732722735368 22.7359124566788 78-79 22.15252735846524 27.34659738728246 27.976865219322182 22.524010034930107 80-81 22.241559853789607 27.419534482429768 27.712981929505265 22.625923734275354 82-83 22.293600168223907 27.415057405466474 27.828505804831536 22.462836621478075 84-85 22.246335755518913 27.500589886727617 27.669431716549187 22.583642641204282 86-87 22.28194911011699 27.421611599302807 27.617974077986446 22.67846521259376 88-89 22.214472528841377 27.6119011115054 27.3688689479493 22.80475741170393 90-91 22.159835086970713 27.566157600830028 27.480442009870483 22.79356530232878 92-93 22.219278787964413 27.244192015522493 27.61134423110433 22.92518496540876 94-95 22.085744385958055 27.20632802120769 27.659261338403233 23.048666254431023 96-97 22.29974288591778 26.897561501693172 27.74406705546567 23.05862855692337 98-99 22.000094660422217 27.11651402869812 27.441467716910704 23.44192359396895 100-101 22.26821408429478 26.81062344509607 27.565902023832933 23.355260446776217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 790.0 1 520.0 2 347.0 3 463.5 4 378.5 5 343.5 6 778.0 7 1808.0 8 2928.5 9 3431.0 10 2892.0 11 2209.5 12 2111.5 13 2229.0 14 2438.0 15 2615.5 16 2799.0 17 2975.0 18 3195.0 19 3529.5 20 4055.0 21 4767.0 22 5761.0 23 7059.5 24 8808.0 25 11163.5 26 14017.5 27 17265.5 28 20188.5 29 23026.0 30 26359.0 31 30684.0 32 36540.5 33 39791.5 34 41382.5 35 44279.5 36 48330.5 37 52503.0 38 55535.5 39 58249.0 40 61280.5 41 65002.5 42 67067.5 43 68846.5 44 72031.0 45 75722.0 46 77131.5 47 78899.0 48 80434.5 49 78387.5 50 76269.5 51 74140.0 52 71905.5 53 71642.5 54 72412.0 55 68486.0 56 62107.0 57 56226.0 58 51804.0 59 46301.5 60 37039.0 61 29920.5 62 24957.5 63 18798.0 64 13270.5 65 9827.0 66 7135.5 67 5275.0 68 3888.0 69 2790.5 70 2022.5 71 1329.0 72 897.5 73 686.0 74 484.0 75 312.0 76 210.5 77 126.0 78 79.5 79 48.5 80 17.5 81 8.5 82 3.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009842783897205543 3 0.005227507237271844 4 0.0 5 5.180412577476602E-4 6 9.418931959048368E-5 7 0.0 8 2.3547329897620917E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.354732989762092E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014834817835501178 26-27 0.021922564134685078 28-29 2.3547329897620917E-4 30-31 2.354732989762092E-5 32-33 0.0032730788557693074 34-35 0.015965089670586984 36-37 0.007605787556931557 38-39 0.021098407588268343 40-41 0.02328830926874709 42-43 0.01926171585625391 44-45 0.00974859457761506 46-47 0.0024018276495573337 48-49 2.1192596907858825E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8837863918096736E-4 68-69 1.6483130928334643E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1192596907858825E-4 82-83 0.0010596298453929413 84-85 0.004426898020752732 86-87 6.593252371333857E-4 88-89 7.064198969286277E-5 90-91 0.0038617621032098304 92-93 6.122305773381438E-4 94-95 9.418931959048368E-5 96-97 3.061152886690719E-4 98-99 1.6483130928334643E-4 100-101 0.002849226917612131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2123383.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.37875425357311 #Duplication Level Percentage of deduplicated Percentage of total 1 74.94016880109466 41.50093191757108 2 14.228109343258573 15.758699416265683 3 4.750470454453245 7.89225407658078 4 2.108216957430548 4.670017151950477 5 1.1556092173424624 3.199809943018609 6 0.6472680606630898 2.1506939320588865 7 0.43324676021124003 1.6794866105426485 8 0.29327356726278503 1.2992899848411616 9 0.21981947141346253 1.0955995638800833 >10 1.1174559869878755 11.032210739970193 >50 0.06770715470063968 2.5829261691942116 >100 0.03354844382710189 3.4456717210051115 >500 0.003403854236182535 1.3689428924278209 >1k 0.0014466380503775423 1.2447677221449223 >5k 2.5528906771368396E-4 1.0786981585483937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9482 0.4465515641784831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8272 0.3895670258262405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 0.2418781727083621 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2231 0.10506818600318453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.064198969286275E-5 0.0 0.0 0.0 4.709465979524184E-5 14-15 9.418931959048368E-5 0.0 0.0 0.0 7.064198969286275E-5 16-17 9.418931959048368E-5 0.0 0.0 2.354732989762092E-5 9.418931959048368E-5 18-19 1.1773664948810459E-4 0.0 0.0 4.709465979524184E-5 9.418931959048368E-5 20-21 1.412839793857255E-4 0.0 0.0 4.709465979524184E-5 9.418931959048368E-5 22-23 1.412839793857255E-4 0.0 0.0 1.412839793857255E-4 9.418931959048368E-5 24-25 1.412839793857255E-4 0.0 0.0 4.944939278500394E-4 9.418931959048368E-5 26-27 1.412839793857255E-4 0.0 0.0 0.0013186504742667715 9.418931959048368E-5 28-29 2.3547329897620917E-4 0.0 0.0 0.004379803360957491 9.418931959048368E-5 30-31 2.82567958771451E-4 0.0 0.0 0.014246134588060656 9.418931959048368E-5 32-33 2.82567958771451E-4 0.0 0.0 0.03454393295980989 9.418931959048368E-5 34-35 2.82567958771451E-4 0.0 0.0 0.06350714873388362 9.418931959048368E-5 36-37 2.82567958771451E-4 0.0 0.0 0.1100837672713778 9.418931959048368E-5 38-39 3.0611528866907197E-4 0.0 0.0 0.1775233200981641 9.418931959048368E-5 40-41 3.2966261856669286E-4 0.0 0.0 0.25242737650249625 9.418931959048368E-5 42-43 3.2966261856669286E-4 0.0 0.0 0.32787302149447367 9.418931959048368E-5 44-45 3.767572783619347E-4 0.0 0.0 0.41937794547662854 9.418931959048368E-5 46-47 4.4739926805479745E-4 0.0 0.0 0.5248699834179703 1.1773664948810459E-4 48-49 5.180412577476602E-4 0.0 0.0 0.6358014545656625 1.412839793857255E-4 50-51 5.180412577476602E-4 0.0 0.0 0.7540325979816171 1.412839793857255E-4 52-53 5.180412577476602E-4 0.0 0.0 0.869202588510881 1.8837863918096736E-4 54-55 5.180412577476602E-4 0.0 0.0 0.9911777573805574 1.8837863918096736E-4 56-57 5.180412577476602E-4 0.0 0.0 1.1280583860754279 1.8837863918096736E-4 58-59 5.65135917542902E-4 0.0 0.0 1.2756530498737157 1.8837863918096736E-4 60-61 5.65135917542902E-4 0.0 0.0 1.4276746116927563 1.8837863918096736E-4 62-63 5.65135917542902E-4 0.0 0.0 1.5755518434498157 1.8837863918096736E-4 64-65 5.65135917542902E-4 0.0 0.0 1.7311761467431923 1.8837863918096736E-4 66-67 6.122305773381439E-4 0.0 0.0 1.9081814255836087 2.1192596907858828E-4 68-69 6.122305773381439E-4 0.0 0.0 2.106685416620553 2.82567958771451E-4 70-71 6.593252371333857E-4 0.0 0.0 2.3041768724719 2.82567958771451E-4 72-73 7.299672268262485E-4 0.0 0.0 2.507908370746116 2.82567958771451E-4 74-75 7.535145567238694E-4 0.0 0.0 2.713241087453371 2.82567958771451E-4 76-77 7.535145567238694E-4 0.0 0.0 2.9367758901714858 2.82567958771451E-4 78-79 7.535145567238694E-4 0.0 0.0 3.1828454876016243 2.82567958771451E-4 80-81 8.006092165191112E-4 0.0 0.0 3.4360499259907424 2.82567958771451E-4 82-83 8.006092165191112E-4 0.0 0.0 3.70488979143188 2.82567958771451E-4 84-85 8.241565464167321E-4 0.0 0.0 3.96975015812032 2.82567958771451E-4 86-87 8.477038763143531E-4 0.0 0.0 4.245983885149311 2.82567958771451E-4 88-89 8.477038763143531E-4 0.0 0.0 4.542350579240768 2.82567958771451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5240 0.0 51.768234 1 GTATCAA 8865 0.0 38.37009 1 ATCAACG 10655 0.0 31.56216 3 TATCAAC 10820 0.0 31.129887 2 TCAACGC 10880 0.0 30.995306 4 CAACGCA 10960 0.0 30.725727 5 AACGCAG 11365 0.0 29.714378 6 ACGCAGA 12980 0.0 25.761103 7 CGCAGAG 13005 0.0 25.56549 8 GAGTACT 9040 0.0 23.223106 12-13 GCAGAGT 14400 0.0 22.857946 9 GTACATG 7750 0.0 22.313013 1 GTACTTT 9345 0.0 22.1602 14-15 TACATGG 7390 0.0 22.04998 2 CAGAGTA 13710 0.0 21.47933 10-11 ACATGGG 7480 0.0 20.95556 3 GTGGTAT 2500 0.0 20.903126 1 TAAGGTG 1380 0.0 20.650885 5 AGAGTAC 12680 0.0 20.452192 10-11 AGTACTT 9405 0.0 19.72099 12-13 >>END_MODULE