FastQCFastQC Report
Thu 26 May 2016
SRR938081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173065
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103230.47504331439694625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84810.3902782475443671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.2461960410756236No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22280.10252799617130642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.053.127721
GTATCAA86500.039.452251
ATCAACG106800.031.9017643
TCAACGC108550.031.4312344
CAACGCA109550.031.1876965
TATCAAC109700.031.058422
AACGCAG113200.030.1820876
CGCAGAG127150.026.6606128
ACGCAGA127750.026.609837
GTGGTAT24250.024.6025521
ACCGTAT1401.7676748E-523.772078
GCAGAGT139850.023.728489
TGGTATC23700.023.458722
GAGTACT88200.022.82531512-13
TACATGG76050.022.8065952
GTACATG79100.022.6878431
CAGAGTA133300.021.6124710-11
ACATGGG77800.021.4995753
GTACTTT94200.021.12977614-15
GTGTAAG9500.021.101221
Produced by FastQC (version 0.11.5)