##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173065 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733399139004124 33.0 31.0 34.0 30.0 34.0 2 31.936521457020383 33.0 31.0 34.0 30.0 34.0 3 31.999452846555442 34.0 31.0 34.0 30.0 34.0 4 35.520823813369596 37.0 35.0 37.0 33.0 37.0 5 35.540798365442356 37.0 35.0 37.0 33.0 37.0 6 35.56180233909248 37.0 35.0 37.0 33.0 37.0 7 35.49971629932837 37.0 35.0 37.0 33.0 37.0 8 35.47301760416739 37.0 35.0 37.0 33.0 37.0 9 37.112758707171665 39.0 37.0 39.0 33.0 39.0 10-11 37.02680499662919 39.0 37.0 39.0 33.0 39.0 12-13 36.924450027955906 39.0 37.0 39.0 33.0 39.0 14-15 38.14310524535621 40.0 38.0 41.0 33.0 41.0 16-17 38.054914602186315 40.0 38.0 41.0 33.0 41.0 18-19 38.00405234081815 40.0 38.0 41.0 33.0 41.0 20-21 37.89534482401585 40.0 38.0 41.0 33.0 41.0 22-23 37.73848527310504 40.0 38.0 41.0 32.0 41.0 24-25 37.68918578137331 40.0 38.0 41.0 32.0 41.0 26-27 37.69254210067347 40.0 38.0 41.0 32.0 41.0 28-29 37.599685697390555 40.0 38.0 41.0 32.0 41.0 30-31 37.39568213560109 40.0 37.0 41.0 32.0 41.0 32-33 37.15925823663811 40.0 36.5 41.0 31.0 41.0 34-35 36.94208180611256 40.0 36.0 41.0 30.5 41.0 36-37 36.7088883673521 40.0 36.0 41.0 30.0 41.0 38-39 36.46247489145516 39.0 35.5 41.0 30.0 41.0 40-41 36.22712252049524 39.0 35.0 41.0 30.0 41.0 42-43 35.93211868950077 39.0 35.0 40.5 28.5 41.0 44-45 35.686732104193844 39.0 35.0 40.5 27.0 41.0 46-47 35.522691912114915 39.0 35.0 41.0 26.5 41.0 48-49 35.246020942769775 39.0 35.0 41.0 25.5 41.0 50-51 34.60424239495828 38.5 34.0 40.0 24.0 40.5 52-53 34.68563250524029 38.5 34.5 40.0 24.0 41.0 54-55 34.82428735449699 39.0 35.0 40.0 24.0 41.0 56-57 34.612723733528455 38.5 34.0 40.0 23.5 41.0 58-59 34.347164258777354 38.0 34.0 40.0 23.0 41.0 60-61 33.973790015485044 38.0 34.0 40.0 22.0 41.0 62-63 33.54831033586202 37.0 33.0 40.0 20.5 41.0 64-65 33.1145955137099 36.5 33.0 40.0 18.5 41.0 66-67 32.68609521574366 36.0 32.0 39.0 16.0 41.0 68-69 32.201103740569195 35.5 32.0 39.0 11.5 40.5 70-71 31.711984455135948 35.0 31.0 38.5 8.0 40.0 72-73 31.21465027507231 35.0 31.0 37.5 6.5 40.0 74-75 30.684905881784484 35.0 30.5 37.0 2.0 39.0 76-77 30.132908127460524 34.5 30.0 36.5 2.0 39.0 78-79 29.56123332712091 34.0 29.0 36.0 2.0 38.5 80-81 29.02559449441227 34.0 29.0 35.5 2.0 37.0 82-83 28.479899358739843 34.0 28.0 35.0 2.0 37.0 84-85 27.974507895530046 34.0 27.0 35.0 2.0 36.0 86-87 27.464992303497596 33.5 26.0 35.0 2.0 36.0 88-89 26.899907043737763 33.0 25.5 35.0 2.0 35.5 90-91 26.281925989328435 32.5 24.5 35.0 2.0 35.0 92-93 25.663319320867068 32.0 21.5 35.0 2.0 35.0 94-95 25.040526169258627 32.0 19.0 34.0 2.0 35.0 96-97 24.19375214271087 31.5 6.5 34.0 2.0 35.0 98-99 23.106870710264076 31.0 2.0 34.0 2.0 35.0 100-101 20.272999196986746 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.654242185749375 1101 2 0.5742059538378648 1101 3 0.4925402866965385 1101 4 0.4747535932384679 1101 5 0.4555603481757018 1101 6 0.45691751000124015 1101 7 0.5508527506482892 1101 8 0.5365627284427745 1101 9 0.4868998626130292 1101 10-11 0.5972260437441506 1101 12-13 0.5037021525027185 1101 14-15 0.7198558502372379 1101 16-17 0.7812970805636397 1101 18-19 0.7344719587796433 1101 20-21 0.8260959032416579 1101 22-23 0.7423298209585525 1101 24-25 0.7719985283495632 1101 26-27 0.8180426478328258 1101 28-29 0.6637203436847159 1101 30-31 0.8449187413690709 1101 32-33 0.9055182269660875 1101 34-35 0.9408283783821574 1101 36-37 0.8303931245596914 1101 38-39 0.9654777778959058 1101 40-41 0.950037143131425 1101 42-43 1.000987657012388 1101 44-45 0.9193228732455836 1101 46-47 0.980601704379275 1101 48-49 1.092034811788345 1101 50-51 1.1510150619315667 1101 52-53 1.209214605047272 1101 54-55 1.2655705120674625 1101 56-57 1.0541398823582497 1101 58-59 1.1308089051674202 1101 60-61 1.1759267215507876 1101 62-63 1.348328368990785 1101 64-65 1.2223289496260463 1101 66-67 1.017896203482934 1101 68-69 1.1948468021256176 1101 70-71 1.3789338393843273 1101 72-73 1.3554752639613952 1101 74-75 1.2401674109824405 1101 76-77 1.2566398136862276 1101 78-79 1.2279049811843201 1101 80-81 1.4083975216948978 1101 82-83 1.373957074281666 1101 84-85 1.3745757783926997 1101 86-87 1.4493534588154553 1101 88-89 1.4559264774084788 1101 90-91 1.3944191060266853 1101 92-93 1.3479050249427225 1101 94-95 1.4499133947076608 1101 96-97 1.266879151853452 1101 98-99 1.3677669276467341 1101 100-101 1.3632638931410312 1102 1 0.4588508673512166 1102 2 0.31649133811843555 1102 3 0.2366679788071906 1102 4 0.2335171828035172 1102 5 0.18966989552563973 1102 6 0.29253128661890315 1102 7 0.26166144621754484 1102 8 0.24165380143182347 1102 9 0.31359330274472086 1102 10-11 0.42994016338824537 1102 12-13 0.29940879442317225 1102 14-15 0.40813280407181907 1102 16-17 0.45566872061962016 1102 18-19 0.30978341033730317 1102 20-21 0.3832696808798133 1102 22-23 0.40147053085910755 1102 24-25 0.36045906871347455 1102 26-27 0.3966204655600549 1102 28-29 0.46005229822927163 1102 30-31 0.5670254560644281 1102 32-33 0.5267806858659227 1102 34-35 0.4392207077431962 1102 36-37 0.42783172653951596 1102 38-39 0.497098392879856 1102 40-41 0.4859621458179575 1102 42-43 0.5272263055846338 1102 44-45 0.4012926608403049 1102 46-47 0.6359581970003774 1102 48-49 0.5978949432830944 1102 50-51 0.43225756442933516 1102 52-53 0.49016732223871173 1102 54-55 0.5644989378026608 1102 56-57 0.5842964952744083 1102 58-59 0.6885006627661951 1102 60-61 0.6461259943687736 1102 62-63 0.5977090479259246 1102 64-65 0.4458474818409215 1102 66-67 0.5837837698764972 1102 68-69 0.47281520740963145 1102 70-71 0.6425525246996493 1102 72-73 0.722636339493949 1102 74-75 0.5368071899364253 1102 76-77 0.5858874619670118 1102 78-79 0.7061310106308376 1102 80-81 0.6884161998976559 1102 82-83 0.9151974275491526 1102 84-85 0.9163995677949366 1102 86-87 0.8848757771803655 1102 88-89 0.9622348806154193 1102 90-91 0.757917470524287 1102 92-93 0.7953551848335998 1102 94-95 0.7753283778320821 1102 96-97 0.785864534768205 1102 98-99 1.0139605866958306 1102 100-101 0.7291436769501125 1103 1 0.5647216836447626 1103 2 0.44936866138299436 1103 3 0.3578277241859311 1103 4 0.3599848993275785 1103 5 0.28866495186120034 1103 6 0.3795286249139167 1103 7 0.39344037106204155 1103 8 0.39284233027766646 1103 9 0.41739979736465926 1103 10-11 0.3781772351691117 1103 12-13 0.31983798408104747 1103 14-15 0.4009999377051585 1103 16-17 0.4424254371977199 1103 18-19 0.5225738876637536 1103 20-21 0.5954214386534531 1103 22-23 0.6470178587329016 1103 24-25 0.5479091882025884 1103 26-27 0.3807535762089387 1103 28-29 0.5395589377999457 1103 30-31 0.6029939578524619 1103 32-33 0.7261556869553587 1103 34-35 0.6978737390962308 1103 36-37 0.7617033854946484 1103 38-39 0.7731016868300529 1103 40-41 0.7577172699549166 1103 42-43 0.7261381416182289 1103 44-45 0.6160309215359874 1103 46-47 0.6122730317039924 1103 48-49 0.6377343594759921 1103 50-51 0.8069380490195144 1103 52-53 0.9356190538410374 1103 54-55 0.9058108702542782 1103 56-57 0.8703549531788042 1103 58-59 1.0770016440037793 1103 60-61 0.881587405124705 1103 62-63 0.8034025168255212 1103 64-65 0.8095014009269832 1103 66-67 0.9080723944182196 1103 68-69 1.0609561400696563 1103 70-71 0.9777818050980294 1103 72-73 0.9889620984185257 1103 74-75 0.729159388613315 1103 76-77 0.7346266943132633 1103 78-79 0.9538660463530633 1103 80-81 1.0188771213421255 1103 82-83 0.8694176705016368 1103 84-85 1.0208849976432326 1103 86-87 0.9778669942262361 1103 88-89 0.8882290731232523 1103 90-91 0.9993051528737418 1103 92-93 1.0250585325956045 1103 94-95 1.0637822208196255 1103 96-97 1.1433386865473913 1103 98-99 1.2263837691527328 1103 100-101 0.9032423166642225 1104 1 0.4285038171194273 1104 2 0.3796786984814453 1104 3 0.28374621015861123 1104 4 0.29611448368429905 1104 5 0.33138231405268925 1104 6 0.30209034644944666 1104 7 0.32182971299659613 1104 8 0.3646991241580366 1104 9 0.3172522488450866 1104 10-11 0.4245362961773651 1104 12-13 0.2886412311932389 1104 14-15 0.3180753664839031 1104 16-17 0.4139277252207876 1104 18-19 0.44994779470296464 1104 20-21 0.33936088439910606 1104 22-23 0.38960030449565153 1104 24-25 0.4846748885618055 1104 26-27 0.47171365310323665 1104 28-29 0.5546568982396565 1104 30-31 0.37260314185738963 1104 32-33 0.5502484796352078 1104 34-35 0.5314548466171729 1104 36-37 0.540032511296971 1104 38-39 0.544563181747769 1104 40-41 0.6548322143832124 1104 42-43 0.7617238337333347 1104 44-45 0.7718777987501682 1104 46-47 0.5072192693354722 1104 48-49 0.567493048549899 1104 50-51 0.5397281312135789 1104 52-53 0.6654028408848873 1104 54-55 0.5784769490063368 1104 56-57 0.49044612743136895 1104 58-59 0.6384219801503619 1104 60-61 0.7281660318169827 1104 62-63 0.6572290430765193 1104 64-65 0.7704500756971768 1104 66-67 0.6446673003613768 1104 68-69 0.7715034135325851 1104 70-71 0.7397337338188485 1104 72-73 0.7430067419371298 1104 74-75 0.7979609498637608 1104 76-77 0.7960938071871659 1104 78-79 0.9369575653762325 1104 80-81 1.0818705327094875 1104 82-83 1.0149752111000332 1104 84-85 1.0505723106550455 1104 86-87 1.029606447380175 1104 88-89 0.9907096405985634 1104 90-91 1.1160861019711632 1104 92-93 1.145243379025473 1104 94-95 1.08094298744507 1104 96-97 1.1983490676566255 1104 98-99 1.067644333094858 1104 100-101 0.975804536834918 1105 1 0.32640031550596404 1105 2 0.31970790081763667 1105 3 0.245595158074444 1105 4 0.2615379397830111 1105 5 0.19667287063055028 1105 6 0.22195615793658874 1105 7 0.16819164417331223 1105 8 0.19052674592333574 1105 9 0.17052606630603862 1105 10-11 0.21056562296795533 1105 12-13 0.15291103944456097 1105 14-15 0.3471571374700275 1105 16-17 0.3417526178788677 1105 18-19 0.3745083151001509 1105 20-21 0.35889444708411844 1105 22-23 0.44755494086656 1105 24-25 0.450451039542763 1105 26-27 0.3927015543575578 1105 28-29 0.6422019018399467 1105 30-31 0.4774475294083942 1105 32-33 0.5059857163919261 1105 34-35 0.4933045723730132 1105 36-37 0.5191352839632302 1105 38-39 0.6204890335936994 1105 40-41 0.6179688208672829 1105 42-43 0.5935594916970786 1105 44-45 0.6086608525055794 1105 46-47 0.48068836908566936 1105 48-49 0.37964402062766567 1105 50-51 0.17162590525772714 1105 52-53 0.20903377580412297 1105 54-55 0.3726890415292914 1105 56-57 0.5072789184991038 1105 58-59 0.4080163254757636 1105 60-61 0.31865515983611203 1105 62-63 0.4728282910163557 1105 64-65 0.3950417096945671 1105 66-67 0.49791522686216183 1105 68-69 0.5699946261629307 1105 70-71 0.5976627014695843 1105 72-73 0.5107028242903837 1105 74-75 0.5074264848843413 1105 76-77 0.5037020825158613 1105 78-79 0.5154256205984282 1105 80-81 0.5629389070683253 1105 82-83 0.42764800270114023 1105 84-85 0.6003282911334828 1105 86-87 0.7055321881061012 1105 88-89 0.8155592952376018 1105 90-91 0.9476220692929935 1105 92-93 1.0092782795952964 1105 94-95 1.2057597505638995 1105 96-97 1.255187392500389 1105 98-99 1.148175108890257 1105 100-101 1.1850198296138963 1106 1 0.12424733526026444 1106 2 0.13968387560171536 1106 3 0.07756347350146342 1106 4 0.18140117761365104 1106 5 0.12648862804764605 1106 6 0.14178308349008262 1106 7 0.17210809215703193 1106 8 0.09074884194397015 1106 9 0.19779194184359739 1106 10-11 -0.5142224674496205 1106 12-13 -0.15696632217293427 1106 14-15 0.023681778094605477 1106 16-17 0.19649390346014428 1106 18-19 0.2910962699800379 1106 20-21 0.0500539909850275 1106 22-23 -0.06517441233156518 1106 24-25 0.12081789910081397 1106 26-27 0.014039661770119949 1106 28-29 0.030799002346839188 1106 30-31 0.17933967350057856 1106 32-33 0.10699961006029213 1106 34-35 -0.014591236725756573 1106 36-37 -0.09741393078378024 1106 38-39 0.09699024013244184 1106 40-41 0.40191594428046784 1106 42-43 0.08869776335935597 1106 44-45 0.0430702185396612 1106 46-47 0.355020867095881 1106 48-49 0.3145554250049827 1106 50-51 0.35328229400176525 1106 52-53 0.23221536463514525 1106 54-55 0.07875519743141268 1106 56-57 0.22354618530355452 1106 58-59 0.3891033292695525 1106 60-61 0.13841991117090657 1106 62-63 0.3864931075865812 1106 64-65 0.2993667763550434 1106 66-67 0.1743409587551099 1106 68-69 -0.12001369188195099 1106 70-71 0.06687347946012778 1106 72-73 0.31335805399720584 1106 74-75 0.3359724737499832 1106 76-77 0.5298479776451614 1106 78-79 0.7387845484573354 1106 80-81 0.4805386444376687 1106 82-83 0.5727284280614704 1106 84-85 0.5737830665146042 1106 86-87 0.5158707367550512 1106 88-89 0.3968462276735423 1106 90-91 0.37604835431481476 1106 92-93 0.3244902736248818 1106 94-95 0.5755569084948604 1106 96-97 0.5080201723529889 1106 98-99 0.566013409475687 1106 100-101 0.44633395403411313 1107 1 0.2549588613223577 1107 2 0.13208824273656106 1107 3 0.12901731649908754 1107 4 0.023990655844656317 1107 5 0.17611299670098646 1107 6 0.0768026174216132 1107 7 -0.051867679552266566 1107 8 0.0877580713381434 1107 9 0.15711352767389286 1107 10-11 0.09351933060049333 1107 12-13 0.027055665350992797 1107 14-15 0.0763690066288163 1107 16-17 0.08004651407539143 1107 18-19 0.1532747547637925 1107 20-21 0.21911474936026565 1107 22-23 0.18793840846571186 1107 24-25 0.14571928529987588 1107 26-27 0.3022852350165621 1107 28-29 0.31135256143269885 1107 30-31 0.4041601584204386 1107 32-33 0.3052548529077157 1107 34-35 0.4511003659456989 1107 36-37 0.3339969104203675 1107 38-39 0.342170781968413 1107 40-41 0.3457993118980056 1107 42-43 0.40994714425706036 1107 44-45 0.3686164349443928 1107 46-47 0.40417631124253006 1107 48-49 0.2678318382989886 1107 50-51 0.17289132426037668 1107 52-53 0.38817387268415615 1107 54-55 0.4913708992426322 1107 56-57 0.36961577574599147 1107 58-59 0.363835497451376 1107 60-61 0.4202465884906559 1107 62-63 0.40870309528517623 1107 64-65 0.4638854186612349 1107 66-67 0.446450139486565 1107 68-69 0.521956251316027 1107 70-71 0.682937999066251 1107 72-73 0.6912527875265297 1107 74-75 0.7298424069951182 1107 76-77 0.7533733143207613 1107 78-79 0.7861048140605824 1107 80-81 0.7907936732792784 1107 82-83 0.6308886389516069 1107 84-85 0.6664838749766488 1107 86-87 0.7320668660035459 1107 88-89 0.8331509766750109 1107 90-91 0.9106831695204001 1107 92-93 0.8983741093801392 1107 94-95 0.7961350428440923 1107 96-97 1.0073745160219367 1107 98-99 1.0070320889575228 1107 100-101 0.9209282379545272 1108 1 0.20616734579991913 1108 2 0.12017639010663217 1108 3 0.1233216797286687 1108 4 0.08597426014873832 1108 5 0.13973958330293357 1108 6 0.15748879383136938 1108 7 0.12487429479059386 1108 8 0.05573802649782067 1108 9 0.053672897729832414 1108 10-11 0.1850215227456502 1108 12-13 0.018941720939636753 1108 14-15 0.09198883041627681 1108 16-17 0.19584896477545755 1108 18-19 0.18604418083901209 1108 20-21 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2316 98-99 -1.9353107535278653 2316 100-101 -1.94570301511391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7677.0 3 2239.0 4 2246.0 5 2466.0 6 2770.0 7 3385.0 8 4038.0 9 5050.0 10 6111.0 11 7459.0 12 9074.0 13 11253.0 14 12950.0 15 15413.0 16 18827.0 17 24920.0 18 22252.0 19 17440.0 20 17344.0 21 17671.0 22 18790.0 23 20830.0 24 22829.0 25 25618.0 26 29006.0 27 33391.0 28 38408.0 29 45293.0 30 53826.0 31 65925.0 32 81524.0 33 105073.0 34 143213.0 35 207646.0 36 306586.0 37 414368.0 38 312707.0 39 37446.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43066469839579 18.66902156218552 14.88000762150003 23.020306117918665 2 15.209303160737628 19.553884381763663 41.15565749981699 24.081154957681726 3 19.091050155101545 23.947061496830052 29.880012137380785 27.081876210687618 4 12.541415220539648 16.611308225943482 37.26285397206214 33.58442258145473 5 13.487650198729787 37.37197702748136 34.512432701297385 14.62794007249147 6 32.12347727621379 36.27771515074177 17.247393581514526 14.351413991529919 7 28.42487863597054 33.04969777570656 20.371934355207255 18.153489233115643 8 26.186968280292195 35.2305427074691 19.422347168136458 19.160141844102245 9 26.22932026024025 16.870726563115074 20.273684123291375 36.626269053353305 10-11 24.949152089651662 26.638801727804278 27.0036540495384 21.408392133005652 12-13 25.95057651670491 23.995224601731508 27.52467051087916 22.529528370684417 14-15 22.618256107454737 25.418605486372474 26.197202942739246 25.765935463433546 16-17 21.867388730273003 28.59536265531048 27.04522206517958 22.492026549236936 18-19 21.808295349320154 26.905482084239097 29.60775357350557 21.678468992935183 20-21 22.43155649043305 26.10546385236304 30.02144922130001 21.441530435903903 22-23 22.149721637663873 26.054023935937522 29.863236370836653 21.933018055561952 24-25 21.897685657080032 26.19693217257692 29.992312698602053 21.913069471740993 26-27 21.491988087930487 26.556436583702457 29.849086770502016 22.102488557865037 28-29 21.573445049612666 26.837763878479766 29.762257534574598 21.82653353733297 30-31 21.812608350167018 26.453846541740127 29.62065899927216 22.1128861088207 32-33 21.455664243691064 26.472695070489177 29.669949875742773 22.401690810076982 34-35 22.207091527069117 26.672056305220863 29.396272215277296 21.72457995243272 36-37 22.31456774398248 26.18490578983857 29.40821885888657 22.09230760729238 38-39 21.789076827002688 26.57409071960965 29.381568949614874 22.25526350377279 40-41 22.323643734724048 26.368626454395077 29.360284845747685 21.94744496513319 42-43 21.985776748586364 26.41636905412731 29.225731677982274 22.372122519304053 44-45 22.020934839087847 26.703070079446338 29.25627997994107 22.019715101524742 46-47 22.33559866289705 27.00183386744416 28.560759287406245 22.101808182252544 48-49 22.148227934627254 26.96581993265714 28.54835405863846 22.337598074077142 50-51 22.2664671120359 27.241738739781667 28.429676440255935 22.062117707926497 52-53 22.581343030247236 27.006772112933756 28.29196609915938 22.119918757659622 54-55 22.161371452980124 27.152492395955313 28.447412415398098 22.23872373566647 56-57 22.20045564062985 27.30660876837792 28.296369397096633 22.196566193895595 58-59 22.202282047859487 26.91707209301255 28.28830300882349 22.592342850304473 60-61 21.939184623401108 27.176051488553902 28.557721165654904 22.327042722390082 62-63 22.077914605996867 27.118360884363916 28.656124935849785 22.14759957378943 64-65 21.82886605720114 27.15781542418488 28.525407642590906 22.487910876023076 66-67 21.761167609881106 27.180339594018903 28.465599674508173 22.592893121591818 68-69 22.187377742593213 26.88107259731114 28.568420422797757 22.363129237297887 70-71 22.054330872509176 26.92853052152408 28.198779838797293 22.81835876716945 72-73 22.02439044600883 27.056306750070437 28.188523020434168 22.73077978348657 74-75 22.02699029425649 27.386312712800077 28.079448584693196 22.507248408250238 76-77 22.107788648085116 27.05235928628799 28.152891601479347 22.68696046414755 78-79 22.199445079458297 27.210390918210088 28.083066408897324 22.507097593434292 80-81 22.285515191808535 27.286618657288475 27.84854608354902 22.579320067353965 82-83 22.276077256671954 27.29450677114354 27.929850934501577 22.499565037682924 84-85 22.298170550970326 27.32572643136851 27.8541991534863 22.52190386417487 86-87 22.294021799284312 27.24816193517374 27.75026295872808 22.707553306813868 88-89 22.299564466486984 27.347642108892817 27.59690971697112 22.75588370764908 90-91 22.284349056425683 27.35552863597374 27.618754261819483 22.741368045781094 92-93 22.371719432486532 27.03929004885794 27.77435721327236 22.81463330538317 94-95 22.219528697662458 26.89599380811653 27.909702927262636 22.974774566958374 96-97 22.453271506708614 26.6041249107141 27.88588254982343 23.056721032753856 98-99 22.19993702654828 26.766087691452185 27.61169501699547 23.42228026500407 100-101 22.614785364448288 26.203353500432154 27.861112071449153 23.320749063670412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 852.0 1 562.5 2 404.5 3 532.5 4 430.0 5 408.0 6 820.5 7 1868.5 8 3095.0 9 3650.5 10 3066.0 11 2294.0 12 2213.0 13 2388.5 14 2541.5 15 2643.5 16 2819.5 17 3093.0 18 3346.5 19 3716.5 20 4271.5 21 4995.5 22 6125.0 23 7528.0 24 9337.0 25 11665.5 26 14558.0 27 17967.5 28 20966.5 29 23675.0 30 26941.0 31 31440.0 32 37133.0 33 40472.0 34 42258.5 35 45289.0 36 49510.5 37 53740.5 38 56791.5 39 59394.5 40 62540.5 41 66323.0 42 68220.5 43 70181.5 44 73188.5 45 76757.5 46 78703.5 47 80286.5 48 81525.0 49 79647.0 50 77291.0 51 75032.5 52 73400.0 53 72896.0 54 73041.0 55 69271.0 56 62896.0 57 57238.5 58 52944.5 59 47099.0 60 37977.0 61 31071.5 62 26015.5 63 19924.0 64 14398.0 65 10897.5 66 8078.0 67 5949.0 68 4479.5 69 3303.5 70 2495.0 71 1693.5 72 1118.0 73 847.0 74 632.5 75 444.5 76 292.5 77 183.0 78 131.0 79 80.5 80 46.5 81 29.5 82 23.5 83 18.0 84 13.5 85 11.5 86 5.5 87 5.5 88 8.0 89 6.5 90 6.0 91 4.0 92 3.0 93 2.5 94 1.0 95 1.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49519917719902534 2 0.04933124411833056 3 0.05807465492288542 4 0.06718620934026363 5 0.08168186409518353 6 0.10887847349250943 7 0.06898090945277753 8 0.05075780061802109 9 0.04933124411833056 10-11 0.07540041370138491 12-13 0.10841829397648022 14-15 0.05572773939113648 16-17 0.06104281280127378 18-19 0.06065166021264896 20-21 0.0762747547818404 22-23 0.06603576055019063 24-25 0.08982704152890042 26-27 0.06838267608193957 28-29 0.06868179276735854 30-31 0.07250128275040094 32-33 0.07185703142796004 34-35 0.07429598286291482 36-37 0.06951011589621112 38-39 0.039874555063930436 40-41 0.043348910409950925 42-43 0.04222147059567938 44-45 0.021467374422762323 46-47 0.027932896622972624 48-49 0.02761077096175218 50-51 0.02579306187343683 52-53 0.025424918260613466 54-55 0.0247116400107682 56-57 0.023906325857717094 58-59 0.020938167979328737 60-61 0.02857714794541351 62-63 0.019787719189255728 64-65 0.020754096172917055 66-67 0.016244336915830864 68-69 0.01817709088315352 70-71 0.041094030781407824 72-73 0.037780738265997565 74-75 0.031821413533419385 76-77 0.022088616769401746 78-79 0.014794771440338876 80-81 0.01677354335926445 82-83 0.0213063115921521 84-85 0.017900983173535997 86-87 0.008996509538370919 88-89 0.041485183370032645 90-91 0.05356489566579923 92-93 0.03435240087158001 94-95 0.05473835343167369 96-97 0.04461440407903123 98-99 0.03317894310570554 100-101 0.1698062414147759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.96449349211543 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46435726803904 43.55753381973999 2 13.35303313175474 15.21297537867689 3 4.466166501767336 7.632387378738881 4 2.0064513023829513 4.5718592862736065 5 1.0258699993450227 2.9219082450723 6 0.6568492072055832 2.245024943349817 7 0.3970886022413295 1.5833965768718599 8 0.2921548707281699 1.331396338582773 9 0.20378849512231817 1.0447837563746962 >10 1.0357276612414072 10.554285435182202 >50 0.06292668210198615 2.4550534321988504 >100 0.030806539617577403 3.2310757146389655 >500 0.003159488129602311 1.3233044950238864 >1k 0.0013772127744420067 1.2203474826996048 >5k 1.620250322872949E-4 0.6382780983670486 >10k+ 8.101251614364745E-5 0.47638961820859255 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10323 0.47504331439694625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8481 0.3902782475443671 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5350 0.2461960410756236 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2228 0.10252799617130642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 4.60179516029203E-5 0.0 0.0 7 0.0 0.0 4.60179516029203E-5 0.0 0.0 8 0.0 0.0 4.60179516029203E-5 0.0 0.0 9 0.0 0.0 4.60179516029203E-5 0.0 0.0 10-11 0.0 0.0 4.60179516029203E-5 0.0 0.0 12-13 0.0 0.0 4.60179516029203E-5 2.300897580146015E-5 0.0 14-15 0.0 0.0 4.60179516029203E-5 4.60179516029203E-5 0.0 16-17 0.0 0.0 4.60179516029203E-5 6.902692740438045E-5 0.0 18-19 2.300897580146015E-5 0.0 4.60179516029203E-5 1.6106283061022105E-4 0.0 20-21 4.60179516029203E-5 0.0 4.60179516029203E-5 1.840718064116812E-4 0.0 22-23 4.60179516029203E-5 0.0 4.60179516029203E-5 2.300897580146015E-4 0.0 24-25 1.380538548087609E-4 0.0 4.60179516029203E-5 3.681436128233624E-4 0.0 26-27 1.380538548087609E-4 0.0 4.60179516029203E-5 8.283231288525653E-4 0.0 28-29 2.761077096175218E-4 0.0 4.60179516029203E-5 0.004210642571667207 0.0 30-31 3.221256612204421E-4 0.0 4.60179516029203E-5 0.014518663730721353 0.0 32-33 3.221256612204421E-4 0.0 4.60179516029203E-5 0.034375409847381466 0.0 34-35 3.681436128233624E-4 0.0 4.60179516029203E-5 0.06318264755080957 0.0 36-37 4.60179516029203E-4 0.0 4.60179516029203E-5 0.11106432619364814 0.0 38-39 4.60179516029203E-4 0.0 4.60179516029203E-5 0.18174789985573372 0.0 40-41 4.60179516029203E-4 0.0 4.60179516029203E-5 0.2541571467029288 0.0 42-43 4.831884918306631E-4 0.0 4.60179516029203E-5 0.3277628602917998 0.0 44-45 5.061974676321232E-4 0.0 4.60179516029203E-5 0.4209492122877134 4.60179516029203E-5 46-47 5.982333708379638E-4 0.0 4.60179516029203E-5 0.526284303506798 9.20359032058406E-5 48-49 5.982333708379638E-4 0.0 4.60179516029203E-5 0.6338972833302271 9.20359032058406E-5 50-51 5.982333708379638E-4 0.0 4.60179516029203E-5 0.74944835980516 9.20359032058406E-5 52-53 5.982333708379638E-4 0.0 4.60179516029203E-5 0.8624224309903293 9.20359032058406E-5 54-55 5.982333708379638E-4 0.0 4.60179516029203E-5 0.9796531626987688 9.20359032058406E-5 56-57 6.442513224408842E-4 0.0 4.60179516029203E-5 1.1160503712498246 9.20359032058406E-5 58-59 6.442513224408842E-4 0.0 4.60179516029203E-5 1.2637679958951988 9.20359032058406E-5 60-61 6.442513224408842E-4 0.0 4.60179516029203E-5 1.4177210529827686 9.20359032058406E-5 62-63 6.442513224408842E-4 0.0 4.60179516029203E-5 1.5687289611677517 9.20359032058406E-5 64-65 6.442513224408842E-4 0.0 4.60179516029203E-5 1.7244307004162325 9.20359032058406E-5 66-67 6.902692740438044E-4 0.0 4.60179516029203E-5 1.9042458463046434 9.20359032058406E-5 68-69 6.902692740438044E-4 0.0 4.60179516029203E-5 2.0988097456817902 1.380538548087609E-4 70-71 7.132782498452646E-4 0.0 4.60179516029203E-5 2.3012887327346396 1.380538548087609E-4 72-73 8.053141530511052E-4 0.0 4.60179516029203E-5 2.50961199964106 1.380538548087609E-4 74-75 8.743410804554856E-4 0.0 4.60179516029203E-5 2.7155423330641284 1.380538548087609E-4 76-77 8.743410804554856E-4 0.0 4.60179516029203E-5 2.9324479479444934 1.380538548087609E-4 78-79 8.743410804554856E-4 0.0 4.60179516029203E-5 3.173259888682575 1.380538548087609E-4 80-81 9.20359032058406E-4 0.0 4.60179516029203E-5 3.4255072903939827 1.380538548087609E-4 82-83 9.663769836613262E-4 0.0 4.60179516029203E-5 3.6888680274174956 1.380538548087609E-4 84-85 9.663769836613262E-4 0.0 4.60179516029203E-5 3.957934990439771 1.6106283061022105E-4 86-87 9.663769836613262E-4 0.0 4.60179516029203E-5 4.230200201098448 2.0708078221314135E-4 88-89 9.663769836613262E-4 0.0 4.60179516029203E-5 4.522253130946382 2.300897580146015E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4995 0.0 53.12772 1 GTATCAA 8650 0.0 39.45225 1 ATCAACG 10680 0.0 31.901764 3 TCAACGC 10855 0.0 31.431234 4 CAACGCA 10955 0.0 31.187696 5 TATCAAC 10970 0.0 31.05842 2 AACGCAG 11320 0.0 30.182087 6 CGCAGAG 12715 0.0 26.660612 8 ACGCAGA 12775 0.0 26.60983 7 GTGGTAT 2425 0.0 24.602552 1 ACCGTAT 140 1.7676748E-5 23.77207 8 GCAGAGT 13985 0.0 23.72848 9 TGGTATC 2370 0.0 23.45872 2 GAGTACT 8820 0.0 22.825315 12-13 TACATGG 7605 0.0 22.806595 2 GTACATG 7910 0.0 22.687843 1 CAGAGTA 13330 0.0 21.61247 10-11 ACATGGG 7780 0.0 21.499575 3 GTACTTT 9420 0.0 21.129776 14-15 GTGTAAG 950 0.0 21.10122 1 >>END_MODULE