FastQCFastQC Report
Thu 26 May 2016
SRR938081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173065
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101120.46533352660873006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86360.39741103004281975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.25199430297759157No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23020.10593332458992252No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22020.1013315294296305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.049.552931
GTATCAA90700.037.868911
ATCAACG111850.030.4923043
TATCAAC114250.029.9383622
TCAACGC114300.029.8359624
CAACGCA114600.029.716415
AACGCAG120000.028.4187556
ACGCAGA132750.025.4388167
CGCAGAG133300.025.1556998
GTGTAGC13950.022.8166751
GCAGAGT146000.022.7072439
TGGTATC27150.022.2221322
GAGTACT93950.022.06781212-13
GTACTTT96500.021.28779214-15
CAGAGTA138850.021.1573210-11
GTGGTAT30450.021.0619341
AGAGTAC128050.020.73476410-11
GTACATG74400.020.4966981
TACATGG71900.020.4165042
GGACCGA7050.020.2112886
Produced by FastQC (version 0.11.5)