##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938081_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173065 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48686946777938 34.0 31.0 34.0 31.0 34.0 2 32.703739188657494 34.0 31.0 34.0 31.0 34.0 3 32.76163437356913 34.0 31.0 34.0 31.0 34.0 4 36.22935807258411 37.0 35.0 37.0 35.0 37.0 5 36.193441981717065 37.0 35.0 37.0 35.0 37.0 6 36.23015464332636 37.0 37.0 37.0 35.0 37.0 7 36.19214887727703 37.0 37.0 37.0 35.0 37.0 8 36.18192921058505 37.0 37.0 37.0 35.0 37.0 9 37.885593850160944 39.0 38.0 39.0 35.0 39.0 10-11 37.87278521351179 39.0 38.0 39.0 35.0 39.0 12-13 37.82768532004335 39.0 38.0 39.0 35.0 39.0 14-15 39.146244129835054 40.5 38.5 41.0 36.0 41.0 16-17 39.09678495581126 40.0 38.0 41.0 36.0 41.0 18-19 39.12971425152952 40.0 38.5 41.0 36.0 41.0 20-21 39.0610536270199 40.0 39.0 41.0 36.0 41.0 22-23 38.9224848313327 40.0 38.0 41.0 35.0 41.0 24-25 38.89025822973542 40.0 38.0 41.0 35.0 41.0 26-27 38.85706663169302 40.0 38.0 41.0 35.0 41.0 28-29 38.79837303532108 40.0 38.0 41.0 35.0 41.0 30-31 38.598576434667166 40.0 38.0 41.0 34.5 41.0 32-33 38.41887656374752 40.0 38.0 41.0 34.0 41.0 34-35 38.22710664430194 40.0 38.0 41.0 34.0 41.0 36-37 38.09658431754227 40.0 38.0 41.0 33.5 41.0 38-39 38.08062046004146 40.0 38.0 41.0 34.0 41.0 40-41 38.01221799624034 40.0 38.0 41.0 33.0 41.0 42-43 37.861106777754 40.0 38.0 41.0 33.0 41.0 44-45 37.65255572198715 40.0 37.0 41.0 33.0 41.0 46-47 37.33065301774222 40.0 37.0 41.0 32.5 41.0 48-49 36.98408077991225 40.0 37.0 41.0 31.0 41.0 50-51 36.688521282152166 40.0 36.0 41.0 31.0 41.0 52-53 36.27969434876545 39.0 35.5 41.0 30.0 41.0 54-55 36.06245234265887 39.0 35.0 41.0 29.5 41.0 56-57 35.82725712300368 39.0 35.0 40.5 29.0 41.0 58-59 35.50183243483283 38.5 35.0 40.0 28.0 41.0 60-61 35.156717125350596 38.0 35.0 40.0 28.0 41.0 62-63 34.80226638411644 37.5 34.0 40.0 27.0 41.0 64-65 34.42015747343038 37.0 34.0 40.0 26.0 41.0 66-67 34.009834036257544 36.5 34.0 39.5 26.0 41.0 68-69 33.56211664170193 36.0 33.0 39.0 25.5 40.5 70-71 33.056412256421225 35.5 33.0 38.5 24.0 40.0 72-73 32.64495516701065 35.0 32.0 38.0 23.0 40.0 74-75 32.19792965235739 35.0 32.0 37.0 22.0 39.0 76-77 30.89779320913088 34.0 30.5 36.0 20.5 38.5 78-79 31.60267824478329 35.0 31.5 36.0 22.0 39.0 80-81 31.438577539098002 35.0 32.0 36.0 20.5 37.5 82-83 31.065853989641358 35.0 31.0 35.5 19.5 37.0 84-85 30.68347978546431 34.0 31.0 35.0 18.0 37.0 86-87 30.259681371703103 34.0 31.0 35.0 14.0 36.0 88-89 29.793340282044028 34.0 30.0 35.0 7.5 36.0 90-91 29.458247222241397 34.0 30.0 35.0 4.5 35.5 92-93 29.060064931329713 34.0 29.0 35.0 2.0 35.0 94-95 28.655741544776618 34.0 29.0 35.0 2.0 35.0 96-97 28.08929300320055 34.0 29.0 35.0 2.0 35.0 98-99 27.41528394226588 34.0 27.5 35.0 2.0 35.0 100-101 25.013020089136774 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1580033095073219 1101 2 0.21922009787493124 1101 3 0.25272578446091387 1101 4 0.10826407573803465 1101 5 0.10452369017782814 1101 6 0.13820595195615937 1101 7 0.09894033435008254 1101 8 0.13687193766328676 1101 9 0.09826523977478274 1101 10-11 0.18947842050818764 1101 12-13 0.2141300809971156 1101 14-15 0.28642075818790147 1101 16-17 0.3365492445022866 1101 18-19 0.333848815696868 1101 20-21 0.276835663635147 1101 22-23 0.38657427045291115 1101 24-25 0.34744881349816126 1101 26-27 0.37862199420400344 1101 28-29 0.3618028418088386 1101 30-31 0.32528697981658183 1101 32-33 0.4130166372172468 1101 34-35 0.2945342965616433 1101 36-37 0.4406355579606682 1101 38-39 0.41145026902772486 1101 40-41 0.42938054434538486 1101 42-43 0.378592342612329 1101 44-45 0.3735518178026709 1101 46-47 0.4513905084418255 1101 48-49 0.4700596298918782 1101 50-51 0.5224363719870055 1101 52-53 0.46417737716473084 1101 54-55 0.44403539267228354 1101 56-57 0.4473832941194331 1101 58-59 0.530967360631422 1101 60-61 0.527002881897225 1101 62-63 0.46267907607011693 1101 64-65 0.503369495082211 1101 66-67 0.42333179261977705 1101 68-69 0.5922927252786465 1101 70-71 0.5228056087135542 1101 72-73 0.5174733673214362 1101 74-75 0.4162265245947836 1101 76-77 0.3881407138424997 1101 78-79 0.3400780370223977 1101 80-81 0.4596599795338001 1101 82-83 0.47851136859771515 1101 84-85 0.578286834888253 1101 86-87 0.6552206598522012 1101 88-89 0.5765609154282707 1101 90-91 0.5058049642317606 1101 92-93 0.6480907024758409 1101 94-95 0.5634693217467088 1101 96-97 0.6160500566926821 1101 98-99 0.5440226633610514 1101 100-101 0.558853305151036 1102 1 0.11900379939782368 1102 2 0.1533134615723739 1102 3 0.08793471206458747 1102 4 0.12964118602318564 1102 5 0.09160846545827894 1102 6 0.07912840468662807 1102 7 0.1392971581175786 1102 8 0.16971006325616145 1102 9 0.21291044025235095 1102 10-11 0.17465324132191284 1102 12-13 0.190286896466624 1102 14-15 0.23025085663759626 1102 16-17 0.22974306513020792 1102 18-19 0.16701829384365396 1102 20-21 0.1344889721741609 1102 22-23 0.1999734069983603 1102 24-25 0.13281106059315562 1102 26-27 -0.051082713409194014 1102 28-29 0.11870492478406902 1102 30-31 0.14144120280927552 1102 32-33 0.1692761415697035 1102 34-35 0.2782639275150913 1102 36-37 0.08583539127936035 1102 38-39 0.16324496651068188 1102 40-41 0.33865210216882957 1102 42-43 0.1993855786702312 1102 44-45 0.11358785477664668 1102 46-47 0.02804535777794115 1102 48-49 0.02687558903485865 1102 50-51 0.006835382516428012 1102 52-53 -0.013523456679926937 1102 54-55 -0.09102643893062634 1102 56-57 0.06753016334289441 1102 58-59 -0.013079877079377411 1102 60-61 -0.004801474236927561 1102 62-63 -0.08922200058086815 1102 64-65 -0.0011802244226188918 1102 66-67 -0.07512908533306017 1102 68-69 0.11528987587345796 1102 70-71 0.12267871650827544 1102 72-73 -0.09750053138487402 1102 74-75 -0.0723381013940454 1102 76-77 -0.10301819287446179 1102 78-79 0.010883217543565138 1102 80-81 0.19087373543226605 1102 82-83 0.12200418735961804 1102 84-85 0.09801414034361144 1102 86-87 -0.01347394560555415 1102 88-89 -0.13271729109317576 1102 90-91 -0.12730055240944083 1102 92-93 -0.03565160739008277 1102 94-95 -0.165828165998942 1102 96-97 -0.30189160948649985 1102 98-99 -0.10561298541142605 1102 100-101 0.0913738136785085 1103 1 0.13581021657456915 1103 2 0.19319172386672534 1103 3 0.1199645677836898 1103 4 0.05453274688195364 1103 5 0.10175476834282193 1103 6 0.07856538522896273 1103 7 0.15224710367078131 1103 8 0.1636194427718678 1103 9 0.1336736067773714 1103 10-11 0.10673942388813629 1103 12-13 0.10942388052004759 1103 14-15 0.2336227480468125 1103 16-17 0.22589132476260687 1103 18-19 0.17264935996743702 1103 20-21 0.18405248891172477 1103 22-23 0.21434160877496566 1103 24-25 0.22957719735085647 1103 26-27 0.2805760873369536 1103 28-29 0.2415565921907188 1103 30-31 0.30114088758312363 1103 32-33 0.31875195576282067 1103 34-35 0.26431718103795276 1103 36-37 0.313918781906537 1103 38-39 0.23173948335855954 1103 40-41 0.34500792199961694 1103 42-43 0.3651182373098578 1103 44-45 0.4701001920245531 1103 46-47 0.46078856238708 1103 48-49 0.6194469693688234 1103 50-51 0.5687075106129384 1103 52-53 0.45219635286461113 1103 54-55 0.4919737351297897 1103 56-57 0.49691990353966986 1103 58-59 0.6439774012482147 1103 60-61 0.5559245693598527 1103 62-63 0.6588357565996219 1103 64-65 0.5776195904109613 1103 66-67 0.6051125922009675 1103 68-69 0.6468945410910365 1103 70-71 0.5493728858827538 1103 72-73 0.459217515891325 1103 74-75 0.3880754800994666 1103 76-77 0.4043125146948263 1103 78-79 0.34753810006274577 1103 80-81 0.3898671233775737 1103 82-83 0.4102252027518851 1103 84-85 0.5163235281265628 1103 86-87 0.6563394212881519 1103 88-89 0.5866329480967138 1103 90-91 0.5989667829745997 1103 92-93 0.6601842897444428 1103 94-95 0.5286447559027145 1103 96-97 0.4568640154165422 1103 98-99 0.5760613911273218 1103 100-101 0.33887651983240374 1104 1 0.22529172148399113 1104 2 0.1192201470208758 1104 3 0.12885736977138862 1104 4 0.050417582104401504 1104 5 0.08820281435782817 1104 6 0.1278103566120521 1104 7 0.16844989716744152 1104 8 0.16988277532637852 1104 9 0.050303469420008184 1104 10-11 0.17104709508145532 1104 12-13 0.1887346527492042 1104 14-15 0.2838668893210752 1104 16-17 0.2528680807657011 1104 18-19 0.2228724873312089 1104 20-21 0.23452125345524877 1104 22-23 0.296893984462848 1104 24-25 0.20006713726769476 1104 26-27 0.27817444668479396 1104 28-29 0.25522323226351773 1104 30-31 0.26460992566364894 1104 32-33 0.2483690087055308 1104 34-35 0.3050899848112394 1104 36-37 0.39905907409725216 1104 38-39 0.34012353501145753 1104 40-41 0.33302488794235785 1104 42-43 0.36106825127164655 1104 44-45 0.4024782591057061 1104 46-47 0.31251476609237017 1104 48-49 0.30636641557036626 1104 50-51 0.3816368117448903 1104 52-53 0.32883793105915515 1104 54-55 0.4477832621956992 1104 56-57 0.41409244745280915 1104 58-59 0.35147765768915207 1104 60-61 0.44851214394142147 1104 62-63 0.46674344545888147 1104 64-65 0.47450694350354894 1104 66-67 0.44487725065012285 1104 68-69 0.45569514306239256 1104 70-71 0.4027919559709119 1104 72-73 0.489200567044648 1104 74-75 0.3262022170119607 1104 76-77 0.13924981488458954 1104 78-79 0.4160867820512806 1104 80-81 0.3745212700276035 1104 82-83 0.5730367072623999 1104 84-85 0.6614777678551569 1104 86-87 0.5145122653321721 1104 88-89 0.42065831580452695 1104 90-91 0.44518283066881637 1104 92-93 0.6074388995078195 1104 94-95 0.4187380861587542 1104 96-97 0.5689505518871911 1104 98-99 0.6408855058836913 1104 100-101 0.6748850308223204 1105 1 0.0769791898147929 1105 2 0.03900417418749669 1105 3 0.0998763846259152 1105 4 0.022038645122776757 1105 5 0.036143094024644995 1105 6 -0.02589105061163366 1105 7 -0.044467136195223134 1105 8 -0.011990885677612084 1105 9 -0.038501190508341665 1105 10-11 0.03620819685848886 1105 12-13 0.07669568963216022 1105 14-15 0.10132673055281316 1105 16-17 0.06996955999515109 1105 18-19 0.044810353471604 1105 20-21 0.044983646002215494 1105 22-23 0.1286514139460948 1105 24-25 0.1148577649623519 1105 26-27 0.015395091003007622 1105 28-29 -0.04628066582611012 1105 30-31 0.10906103730813754 1105 32-33 0.0693619103598806 1105 34-35 0.19157079216471828 1105 36-37 0.1349028304866522 1105 38-39 0.12216276481650823 1105 40-41 0.12523837613252198 1105 42-43 0.0484195727106993 1105 44-45 0.06666661639651039 1105 46-47 0.009060156390255258 1105 48-49 0.05729682333631558 1105 50-51 0.17907449564849287 1105 52-53 0.03517410461970627 1105 54-55 0.10600125296829788 1105 56-57 0.06997480693045333 1105 58-59 0.07445649599621618 1105 60-61 0.12660039100119036 1105 62-63 0.15892237532882092 1105 64-65 0.05159566615690636 1105 66-67 -0.1009940857234497 1105 68-69 0.051260677194015614 1105 70-71 0.1001070745137298 1105 72-73 0.02913965106025529 1105 74-75 0.10990869130767322 1105 76-77 0.20820633140591482 1105 78-79 0.19679068280778722 1105 80-81 0.1776955239679161 1105 82-83 0.2519176844072355 1105 84-85 0.27434335265749255 1105 86-87 0.33220201384538584 1105 88-89 0.20332459577359074 1105 90-91 0.1180058787348841 1105 92-93 0.2143245614718836 1105 94-95 0.08564977242699001 1105 96-97 0.30227143978044424 1105 98-99 0.36252379250217714 1105 100-101 0.26404783051296477 1106 1 -0.10856324593675737 1106 2 0.0178919629176022 1106 3 0.1069042880774802 1106 4 -0.04123444220191885 1106 5 0.011540470673736536 1106 6 -0.01357949971850303 1106 7 0.03388399085763183 1106 8 -0.07820674527577154 1106 9 -0.10394900322144451 1106 10-11 -0.050233178870136896 1106 12-13 -0.054154651006562915 1106 14-15 -0.21342470979899275 1106 16-17 -0.12777031742979972 1106 18-19 -0.08199169606086798 1106 20-21 -0.014751422157196714 1106 22-23 0.07121119725469072 1106 24-25 0.12004947056267667 1106 26-27 0.015776158795070216 1106 28-29 0.04101900968569083 1106 30-31 0.10807437784762897 1106 32-33 0.04099505502419021 1106 34-35 0.06441512016591844 1106 36-37 0.023298630085101024 1106 38-39 0.08861877101492865 1106 40-41 0.06472607745982373 1106 42-43 0.025058111437104458 1106 44-45 0.04008877406356959 1106 46-47 0.10407619079402508 1106 48-49 0.15597564757855764 1106 50-51 0.16519243876911105 1106 52-53 0.027481813167234748 1106 54-55 -0.3875346339062915 1106 56-57 -0.14810511808047266 1106 58-59 0.0357301913404342 1106 60-61 0.0038563439979029113 1106 62-63 -0.0951919956770908 1106 64-65 -0.23619422509626276 1106 66-67 -0.07361853444185584 1106 68-69 -0.153747566959602 1106 70-71 -0.3742166621040326 1106 72-73 -0.19428672896461308 1106 74-75 -0.22867046417853487 1106 76-77 -0.17394438987836125 1106 78-79 -0.12201189874177487 1106 80-81 -0.2892914437247107 1106 82-83 -0.30750777936598794 1106 84-85 -0.1385012709087512 1106 86-87 0.13364943810156404 1106 88-89 0.20241066642630656 1106 90-91 -0.06684504036685368 1106 92-93 0.07742685442329389 1106 94-95 -0.08961132762388857 1106 96-97 -0.010221039144710176 1106 98-99 0.10655548974223095 1106 100-101 0.050586426030086784 1107 1 0.0621899876557066 1107 2 0.0627533253085133 1107 3 0.0021746022057556047 1107 4 0.046815378666565266 1107 5 0.056019651723836716 1107 6 0.08744816092649899 1107 7 0.031655615968226414 1107 8 0.04606611685924378 1107 9 -0.039978967182705105 1107 10-11 -0.04361571780523121 1107 12-13 0.013270261079931345 1107 14-15 0.05329046222981759 1107 16-17 0.08962192336450414 1107 18-19 0.054464351904755404 1107 20-21 0.11763133589781916 1107 22-23 -0.012125873312548663 1107 24-25 0.056637757599546035 1107 26-27 0.017821605919699834 1107 28-29 -0.011263417407761267 1107 30-31 0.08692979685645952 1107 32-33 0.0471180210346418 1107 34-35 0.13770306381000097 1107 36-37 0.03984503649866866 1107 38-39 0.028528278813361396 1107 40-41 0.1405567170343005 1107 42-43 0.23342483400492142 1107 44-45 0.2373946611511215 1107 46-47 0.24222707433513335 1107 48-49 0.23106769171569397 1107 50-51 0.2232432863934264 1107 52-53 0.3081518165936856 1107 54-55 0.32773466133400575 1107 56-57 0.38505771870823224 1107 58-59 0.3041840622689662 1107 60-61 0.2692014264078466 1107 62-63 0.38237771652049446 1107 64-65 0.2338031627739312 1107 66-67 0.20790798398644483 1107 68-69 0.36480612745346974 1107 70-71 0.32515916082503793 1107 72-73 0.3020620918594119 1107 74-75 0.22721709543525748 1107 76-77 0.2563736054536392 1107 78-79 0.417524495425468 1107 80-81 0.45353680441345645 1107 82-83 0.43312287688835127 1107 84-85 0.5060435281008431 1107 86-87 0.4249072218746548 1107 88-89 0.5228794571793678 1107 90-91 0.35292412052972466 1107 92-93 0.27849790632087945 1107 94-95 0.23318920204050642 1107 96-97 0.28514239111673234 1107 98-99 0.46280937695723523 1107 100-101 0.5460210014232736 1108 1 0.03438774586604154 1108 2 0.01414752887436066 1108 3 0.0420840316258122 1108 4 0.05974444396784406 1108 5 -0.019390594200288547 1108 6 -0.017905281353236546 1108 7 0.07163725900331741 1108 8 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50-51 -0.6042063846437173 2316 52-53 -0.5003694425959822 2316 54-55 -0.49885337759559434 2316 56-57 -0.5537608862890551 2316 58-59 -0.5889604676883735 2316 60-61 -0.47950542572004906 2316 62-63 -0.43229811652225436 2316 64-65 -0.5109434085608413 2316 66-67 -0.44268665494467996 2316 68-69 -0.6158953548468062 2316 70-71 -0.6879244432552127 2316 72-73 -0.6293244320069817 2316 74-75 -0.5618378801012014 2316 76-77 -0.4958629172109035 2316 78-79 -0.4012178098137369 2316 80-81 -0.6225931297412863 2316 82-83 -0.6622606288372594 2316 84-85 -0.45006904664061764 2316 86-87 -0.5304695040649285 2316 88-89 -0.5699325702071469 2316 90-91 -0.5410466444007049 2316 92-93 -0.8099846399283521 2316 94-95 -0.6496666157172655 2316 96-97 -0.6271316558030726 2316 98-99 -0.6196423207716499 2316 100-101 -0.667762214671459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 16.0 5 32.0 6 73.0 7 180.0 8 446.0 9 847.0 10 1494.0 11 2489.0 12 3773.0 13 5581.0 14 7783.0 15 10460.0 16 13192.0 17 18959.0 18 18887.0 19 13080.0 20 11092.0 21 10592.0 22 11103.0 23 12030.0 24 13717.0 25 16115.0 26 19111.0 27 23253.0 28 28389.0 29 34489.0 30 42678.0 31 52788.0 32 66148.0 33 85910.0 34 119869.0 35 181220.0 36 302183.0 37 507189.0 38 476085.0 39 61799.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61533824344877 19.188841567095324 15.271747508703145 22.92407268075276 2 15.46939635889666 19.87662628155681 40.631449206258615 24.022528153287908 3 19.07769722524045 23.952406658539314 30.229935998240148 26.73996011798009 4 12.826307542572357 16.992174647329925 37.02351287237151 33.158004937726204 5 13.376688399421276 37.379570965236184 34.697091295976556 14.546649339365983 6 32.228304261492404 36.46375050907359 16.96626654057748 14.341678688856524 7 28.350509533769124 33.15193056811462 20.301831744563554 18.1957281535527 8 26.26738565412028 35.449063438770104 19.233144474877776 19.050406432231842 9 26.04298920829044 16.87245203416201 20.263987553018627 36.82057120452892 10-11 24.959584734004736 26.707116446125635 26.949769104927835 21.383529714941798 12-13 25.877320742821773 23.97951740974154 27.5543299441112 22.588831903325488 14-15 22.521990828622247 25.469785763426312 26.140152273401856 25.868071134549588 16-17 21.812569285969687 28.65725228813392 27.07860209962086 22.45157632627554 18-19 21.71149965601581 26.985133900734677 29.64987241522918 21.653494028020333 20-21 22.329451719115628 26.114060094843 30.048594956892682 21.507893229148692 22-23 22.103526585721088 26.076187320673792 29.941580210440094 21.878705883165022 24-25 21.89875867280362 26.190998301162494 30.02487624579691 21.88536678023697 26-27 21.46034485734505 26.5747967620001 29.888948340438752 22.075910040216094 28-29 21.60206086936714 26.84755875748684 29.783506096347388 21.76687427679863 30-31 21.793480636796414 26.475162961071113 29.654014030873444 22.07734237125903 32-33 21.510794363052394 26.48915317625344 29.670572150596247 22.329480310097924 34-35 22.20871610779045 26.652677789189706 29.452769933517548 21.6858361695023 36-37 22.268425904066614 26.227469377405388 29.46207225353856 22.042032464989436 38-39 21.76069542836944 26.52597868830729 29.452424260276594 22.260901623046674 40-41 22.327664925090115 26.358282429959534 29.422495241040824 21.891557403909527 42-43 21.93164745766768 26.385242003013026 29.28139927511504 22.401711264204256 44-45 21.96239661738908 26.69841545957747 29.35306829833097 21.986119624702486 46-47 22.319752202420176 26.96602585105639 28.603391340521956 22.110830606001482 48-49 22.10872661425222 26.984443631460632 28.57466297602695 22.3321667782602 50-51 22.342635862249864 27.173669448451843 28.4415100330639 22.042184656234397 52-53 22.58510444924565 27.018036736130764 28.344780298794557 22.052078515829024 54-55 22.173865024746153 27.16237204133332 28.45448709541592 22.2092758385046 56-57 22.267557574209697 27.267085890205767 28.247567376033388 22.217789159551142 58-59 22.203224477868815 26.988723300959705 28.257990442071453 22.550061779100027 60-61 21.906592761836393 27.235793683115784 28.486055410215528 22.3715581448323 62-63 22.010340233725177 27.23082374434267 28.59624539532872 22.162590626603436 64-65 21.76248754639185 27.288645300531737 28.411966508134824 22.53690064494159 66-67 21.660465747688175 27.306914427318098 28.382146875496133 22.650472949497598 68-69 22.137717748769532 27.033393686001837 28.41980239390482 22.409086171323807 70-71 22.00332249610573 27.110371756022023 28.03910605527216 22.847199692600086 72-73 22.027619974552074 27.276312489502153 27.996424405160454 22.69964313078532 74-75 22.005163214169848 27.52533863460136 27.83991735175892 22.629580799469874 76-77 22.09248227733639 27.16181982591409 28.068258427612612 22.67743946913691 78-79 22.139052444358544 27.36498908224098 27.964626000602834 22.531332472797637 80-81 22.223840520186926 27.43558061999986 27.722088386679644 22.61849047313357 82-83 22.23247911704395 27.448202169839455 27.82916195749412 22.490156755622476 84-85 22.260817143139832 27.548789172560024 27.664988109742417 22.525405574557723 86-87 22.244477081495965 27.53262279869594 27.555723879499794 22.667176240308304 88-89 22.244771034072404 27.62806977338674 27.400257746724844 22.72690144581601 90-91 22.16762437869899 27.591586246313383 27.49356782897489 22.747221546012746 92-93 22.21870183920455 27.317182828445986 27.632912866148594 22.83120246620087 94-95 22.032889030010608 27.253027405991077 27.75052748077025 22.963556083228067 96-97 22.259619477558196 26.960169161990095 27.748939861439947 23.031271499011766 98-99 21.95514170077747 27.197276657624137 27.46643565654962 23.381145985048768 100-101 22.054218350578562 26.996891487369222 27.745120371456906 23.20376979059531 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 841.0 1 566.5 2 379.0 3 476.5 4 394.5 5 398.0 6 820.5 7 1870.5 8 3132.0 9 3642.5 10 3007.5 11 2280.0 12 2222.5 13 2344.0 14 2504.5 15 2638.0 16 2767.5 17 3018.0 18 3324.0 19 3614.5 20 4072.0 21 4964.0 22 6009.0 23 7234.0 24 9033.5 25 11222.0 26 14153.5 27 17596.5 28 20688.5 29 23600.0 30 26892.0 31 31410.5 32 37326.5 33 40623.0 34 42044.0 35 45100.5 36 49588.0 37 53819.0 38 56702.0 39 59574.5 40 62933.0 41 66716.5 42 68716.0 43 70574.5 44 73795.0 45 77514.5 46 78857.5 47 80848.0 48 82522.5 49 80461.0 50 78338.5 51 75901.5 52 74072.0 53 73984.5 54 74493.5 55 70248.5 56 63438.5 57 57536.0 58 53278.0 59 47287.5 60 37483.0 61 30368.0 62 25526.0 63 18950.5 64 13010.0 65 9756.0 66 7126.5 67 5215.5 68 3859.5 69 2788.5 70 2009.5 71 1280.5 72 836.0 73 628.0 74 449.0 75 311.0 76 215.5 77 119.0 78 64.5 79 34.0 80 16.0 81 9.0 82 6.0 83 4.5 84 3.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014587690658125735 3 0.007178800450055566 4 0.0 5 4.60179516029203E-5 6 0.0 7 0.0 8 4.1416156442628264E-4 9 3.681436128233624E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.761077096175218E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004992947748916853 26-27 0.01967267431024843 28-29 6.902692740438045E-5 30-31 0.0 32-33 4.371705402277429E-4 34-35 0.017785938294528698 36-37 0.003589400225027783 38-39 0.016612480528654227 40-41 0.014771762464537416 42-43 0.00775402484509207 44-45 0.0031752386606015005 46-47 4.60179516029203E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0708078221314132E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.60179516029203E-5 84-85 0.00310621173319712 86-87 2.991166854189819E-4 88-89 6.902692740438045E-5 90-91 1.840718064116812E-4 92-93 2.761077096175218E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.779207005399485 #Duplication Level Percentage of deduplicated Percentage of total 1 74.58766189161777 40.85852970809677 2 14.32211083529061 15.691077484013185 3 4.905490791708813 8.061566866262943 4 2.09678546820977 4.594409808359059 5 1.1762251371343198 3.221634013601766 6 0.670325567983909 2.2031941829761523 7 0.4383143679095468 1.6807359445210526 8 0.32570663121952126 1.4273560779684373 9 0.22325123906341135 1.1006573254979886 >10 1.1467024775861967 11.268826698429454 >50 0.06724863232559236 2.523224996119675 >100 0.03505310186749252 3.563720404622009 >500 0.003023916245698848 1.1891206637518983 >1k 0.0018479488168159088 1.5013206290638133 >5k 1.6799534698326443E-4 0.6493391172412816 >10k+ 8.399767349163222E-5 0.46528607947447836 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10112 0.46533352660873006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8636 0.39741103004281975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5476 0.25199430297759157 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2302 0.10593332458992252 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2202 0.1013315294296305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.60179516029203E-5 0.0 0.0 0.0 0.0 8 4.60179516029203E-5 0.0 0.0 0.0 0.0 9 4.60179516029203E-5 0.0 0.0 0.0 0.0 10-11 4.60179516029203E-5 0.0 0.0 0.0 0.0 12-13 1.1504487900730075E-4 0.0 0.0 2.300897580146015E-5 0.0 14-15 1.380538548087609E-4 0.0 0.0 4.60179516029203E-5 0.0 16-17 1.380538548087609E-4 0.0 0.0 6.902692740438045E-5 0.0 18-19 2.300897580146015E-4 0.0 0.0 1.6106283061022105E-4 0.0 20-21 2.300897580146015E-4 0.0 0.0 1.840718064116812E-4 0.0 22-23 2.300897580146015E-4 0.0 0.0 2.300897580146015E-4 0.0 24-25 2.761077096175218E-4 0.0 0.0 3.681436128233624E-4 0.0 26-27 2.761077096175218E-4 0.0 0.0 8.743410804554856E-4 2.300897580146015E-5 28-29 2.761077096175218E-4 0.0 0.0 0.004302678474873048 4.60179516029203E-5 30-31 2.761077096175218E-4 0.0 0.0 0.014656717585530114 6.902692740438045E-5 32-33 2.99116685418982E-4 0.0 0.0 0.03462850858119752 9.20359032058406E-5 34-35 3.221256612204421E-4 0.0 0.0 0.06380388989744899 9.20359032058406E-5 36-37 3.221256612204421E-4 0.0 0.0 0.11205371215311093 9.20359032058406E-5 38-39 3.681436128233624E-4 0.0 0.0 0.18317445635542423 9.20359032058406E-5 40-41 3.681436128233624E-4 0.0 0.0 0.25606689169445 9.20359032058406E-5 42-43 3.911525886248226E-4 0.0 0.0 0.329672605283321 9.20359032058406E-5 44-45 4.141615644262827E-4 0.0 0.0 0.4231810829404551 9.20359032058406E-5 46-47 4.141615644262827E-4 0.0 0.0 0.5293444972883922 9.20359032058406E-5 48-49 4.141615644262827E-4 0.0 0.0 0.6377858002406739 1.380538548087609E-4 50-51 4.141615644262827E-4 0.0 0.0 0.7538890921348418 1.840718064116812E-4 52-53 4.141615644262827E-4 0.0 0.0 0.8674613966908491 1.840718064116812E-4 54-55 4.141615644262827E-4 0.0 0.0 0.9852443438185237 1.840718064116812E-4 56-57 4.141615644262827E-4 0.0 0.0 1.122423857546829 1.840718064116812E-4 58-59 4.141615644262827E-4 2.300897580146015E-5 0.0 1.270923787369453 1.840718064116812E-4 60-61 4.141615644262827E-4 4.60179516029203E-5 0.0 1.4253600329488534 1.840718064116812E-4 62-63 4.141615644262827E-4 4.60179516029203E-5 0.0 1.5782086591979532 1.840718064116812E-4 64-65 4.141615644262827E-4 4.60179516029203E-5 0.0 1.7348767754300953 1.840718064116812E-4 66-67 4.141615644262827E-4 4.60179516029203E-5 0.0 1.9157503342053734 1.840718064116812E-4 68-69 5.061974676321232E-4 4.60179516029203E-5 0.0 2.1117177811064094 1.840718064116812E-4 70-71 5.522154192350436E-4 4.60179516029203E-5 0.0 2.3157613785137583 1.840718064116812E-4 72-73 5.98233370837964E-4 4.60179516029203E-5 0.0 2.525810318605288 2.0708078221314135E-4 74-75 6.442513224408842E-4 4.60179516029203E-5 0.0 2.7336503970198773 2.300897580146015E-4 76-77 6.442513224408842E-4 4.60179516029203E-5 0.0 2.952902927431991 2.300897580146015E-4 78-79 6.442513224408842E-4 4.60179516029203E-5 0.0 3.1968901068306748 2.300897580146015E-4 80-81 6.902692740438044E-4 4.60179516029203E-5 0.0 3.4529569985251247 2.300897580146015E-4 82-83 6.902692740438044E-4 4.60179516029203E-5 0.0 3.721609799982973 2.300897580146015E-4 84-85 6.902692740438044E-4 4.60179516029203E-5 0.0 3.9952785581655403 2.300897580146015E-4 86-87 6.902692740438044E-4 4.60179516029203E-5 0.0 4.271386267783062 2.530987338160616E-4 88-89 6.902692740438044E-4 4.60179516029203E-5 0.0 4.570249854468228 2.761077096175218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5455 0.0 49.55293 1 GTATCAA 9070 0.0 37.86891 1 ATCAACG 11185 0.0 30.492304 3 TATCAAC 11425 0.0 29.938362 2 TCAACGC 11430 0.0 29.835962 4 CAACGCA 11460 0.0 29.71641 5 AACGCAG 12000 0.0 28.418755 6 ACGCAGA 13275 0.0 25.438816 7 CGCAGAG 13330 0.0 25.155699 8 GTGTAGC 1395 0.0 22.816675 1 GCAGAGT 14600 0.0 22.707243 9 TGGTATC 2715 0.0 22.222132 2 GAGTACT 9395 0.0 22.067812 12-13 GTACTTT 9650 0.0 21.287792 14-15 CAGAGTA 13885 0.0 21.15732 10-11 GTGGTAT 3045 0.0 21.061934 1 AGAGTAC 12805 0.0 20.734764 10-11 GTACATG 7440 0.0 20.496698 1 TACATGG 7190 0.0 20.416504 2 GGACCGA 705 0.0 20.211288 6 >>END_MODULE