FastQCFastQC Report
Thu 26 May 2016
SRR938080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.4533591964190167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76170.3671703348350505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.2293067195497355No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21010.10127673276728912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.049.4932251
GTATCAA85250.038.5951351
ATCAACG104800.030.9527573
TATCAAC105550.030.7771382
TCAACGC106250.030.4408114
CAACGCA107800.030.0031175
AACGCAG111850.028.9919666
ACGCAGA123950.025.851147
CGCAGAG124300.025.6235058
GTGGTAT24450.023.7590941
GCAGAGT133550.023.3137049
TGGTATC24000.022.7904222
GAGTACT87650.022.15382812-13
GTGTAGC12950.022.0612641
GTACATG71300.021.770931
TACATGG69350.021.6037852
GTACTTT89150.021.43475714-15
CAGAGTA129050.021.17963410-11
TAAGGTG12600.021.1394525
AGAGTAC120200.020.70240810-11
Produced by FastQC (version 0.11.5)