##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938080_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2074514 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.819095942471346 33.0 31.0 34.0 30.0 34.0 2 31.949519742937383 33.0 31.0 34.0 30.0 34.0 3 31.96792790986226 34.0 31.0 34.0 30.0 34.0 4 35.48889860468524 37.0 35.0 37.0 33.0 37.0 5 35.49241123463134 37.0 35.0 37.0 33.0 37.0 6 35.51620572336461 37.0 35.0 37.0 33.0 37.0 7 35.44066995932541 37.0 35.0 37.0 33.0 37.0 8 35.42946347915704 37.0 35.0 37.0 33.0 37.0 9 37.041455492708174 39.0 37.0 39.0 33.0 39.0 10-11 36.94042146738947 39.0 37.0 39.0 33.0 39.0 12-13 36.78133818330462 39.0 37.0 39.0 33.0 39.0 14-15 37.925807442128615 40.0 38.0 41.0 33.0 41.0 16-17 37.74194365523684 40.0 38.0 41.0 32.0 41.0 18-19 37.92885731308634 40.0 38.0 41.0 33.0 41.0 20-21 37.92903856035679 40.0 38.0 41.0 33.0 41.0 22-23 37.80775641909382 40.0 38.0 41.0 32.5 41.0 24-25 37.67944974099957 40.0 38.0 41.0 32.0 41.0 26-27 37.48708082953405 40.0 37.0 41.0 32.0 41.0 28-29 37.29949496604988 40.0 37.0 41.0 31.5 41.0 30-31 37.06464815373624 40.0 37.0 41.0 31.0 41.0 32-33 36.75439476426767 39.5 36.0 41.0 30.0 41.0 34-35 36.503437672630795 39.0 35.5 41.0 30.0 41.0 36-37 36.21465654124292 39.0 35.0 41.0 29.5 41.0 38-39 35.898479595702895 39.0 35.0 40.0 28.5 41.0 40-41 35.76444049064022 39.0 35.0 40.0 27.5 41.0 42-43 35.52904366998728 39.0 35.0 40.0 26.5 41.0 44-45 35.182341261615974 38.5 35.0 40.0 25.5 41.0 46-47 34.77534280318186 38.0 34.0 40.0 24.0 41.0 48-49 34.34406154887361 38.0 33.5 40.0 22.5 41.0 50-51 33.61773793765673 37.5 33.0 39.5 21.0 40.5 52-53 33.68840509150577 38.0 33.0 39.5 21.5 40.5 54-55 33.75621253941887 38.0 33.0 40.0 19.5 41.0 56-57 33.42855531464237 38.0 33.0 40.0 17.5 41.0 58-59 33.00548224789035 37.0 32.5 40.0 13.5 41.0 60-61 32.49015094619752 37.0 31.5 40.0 8.5 41.0 62-63 31.931518418289777 36.0 31.0 39.0 7.0 41.0 64-65 31.37284033754412 35.5 30.5 39.0 2.0 40.0 66-67 30.78264451336554 35.0 29.5 38.5 2.0 40.0 68-69 30.08042871728029 35.0 28.5 38.0 2.0 40.0 70-71 29.479888783589793 34.0 28.0 37.5 2.0 39.5 72-73 28.767993853018105 34.0 26.5 36.5 2.0 39.0 74-75 28.07023307627714 34.0 26.0 36.0 2.0 39.0 76-77 27.29906402174196 33.0 24.5 35.5 2.0 38.5 78-79 26.515078953431985 32.5 22.5 35.0 2.0 37.0 80-81 25.776324478889997 32.0 20.5 35.0 2.0 36.5 82-83 25.018198238237968 31.5 17.5 35.0 2.0 36.0 84-85 24.253981655462436 31.0 9.5 34.0 2.0 36.0 86-87 23.484426472899194 30.5 4.5 34.0 2.0 35.0 88-89 22.583124770428157 29.5 2.0 34.0 2.0 35.0 90-91 21.662087843224967 29.0 2.0 34.0 2.0 35.0 92-93 20.705591767517596 28.5 2.0 34.0 2.0 35.0 94-95 19.601868437619608 26.5 2.0 33.5 2.0 35.0 96-97 18.323531921211426 24.5 2.0 33.0 2.0 35.0 98-99 16.777182270160626 11.0 2.0 32.0 2.0 34.0 100-101 13.019397796303135 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21246157063722038 1101 2 0.2822147399510797 1101 3 0.18085507987273886 1101 4 0.15136412775061814 1101 5 0.2663819276519064 1101 6 0.243647357392625 1101 7 0.30289692075491104 1101 8 0.3484950688764812 1101 9 0.2368156258133709 1101 10-11 0.2900024200498521 1101 12-13 0.27226525695829196 1101 14-15 0.22783608682877343 1101 16-17 0.4001571328901008 1101 18-19 0.4379857113977721 1101 20-21 0.4798202736929298 1101 22-23 0.4019935948254343 1101 24-25 0.38863824135676595 1101 26-27 0.37947070368290525 1101 28-29 0.5092723956895355 1101 30-31 0.37721978763194386 1101 32-33 0.3722666289602401 1101 34-35 0.45583971746764007 1101 36-37 0.41922193110545436 1101 38-39 0.4590464116433779 1101 40-41 0.6321884112685368 1101 42-43 0.5206437600909624 1101 44-45 0.5875076776001862 1101 46-47 0.4812603431002245 1101 48-49 0.5918411952493514 1101 50-51 0.5075554714629718 1101 52-53 0.6269635719765887 1101 54-55 0.4153926232098968 1101 56-57 0.33532308002247646 1101 58-59 0.42230864230001686 1101 60-61 0.2954598489775506 1101 62-63 0.07803798443939414 1101 64-65 0.08809479788594032 1101 66-67 -0.08766859075664613 1101 68-69 -0.07539899837833985 1101 70-71 -0.15806715447335407 1101 72-73 -0.20119821933217352 1101 74-75 -0.09872543120139454 1101 76-77 -0.5906012101143432 1101 78-79 -0.7149293028426058 1101 80-81 -0.6243730239489445 1101 82-83 -0.7395059084974669 1101 84-85 -0.5666583499802513 1101 86-87 -0.5818780419869718 1101 88-89 -0.665940161695687 1101 90-91 -0.7075828018255059 1101 92-93 -1.0134015445008835 1101 94-95 -1.2337200418407832 1101 96-97 -1.1398670319927078 1101 98-99 -1.1236182792578173 1101 100-101 -1.095174260965587 1102 1 0.2713165733124505 1102 2 0.2806096035144847 1102 3 0.30070526713865675 1102 4 0.213964448777908 1102 5 0.20645683401894388 1102 6 0.26718935846272274 1102 7 0.2986165569239887 1102 8 0.3147872037079438 1102 9 0.3074416290236428 1102 10-11 0.41413297114669234 1102 12-13 0.3447639193445937 1102 14-15 0.43677134632583403 1102 16-17 0.3918639279676839 1102 18-19 0.31786800139242644 1102 20-21 0.3693333823071683 1102 22-23 0.41456716357877355 1102 24-25 0.45150608512349066 1102 26-27 0.3438901793383309 1102 28-29 0.28936870387573066 1102 30-31 0.39808656130770714 1102 32-33 0.32277492216515924 1102 34-35 0.3672896907153671 1102 36-37 0.3352197909235315 1102 38-39 0.22335887820302958 1102 40-41 0.24000007525997802 1102 42-43 0.48773846314071534 1102 44-45 0.32292768830109964 1102 46-47 0.4157172719391653 1102 48-49 0.42651214227984013 1102 50-51 0.2199685265726643 1102 52-53 0.22942478117937526 1102 54-55 0.09142258575671036 1102 56-57 -0.0012205261840492199 1102 58-59 0.07720430843163939 1102 60-61 0.17774984362709745 1102 62-63 0.10746548577701631 1102 64-65 0.13143348167406543 1102 66-67 -0.17568357203005647 1102 68-69 -0.06630322523762189 1102 70-71 -0.31055511595008056 1102 72-73 -0.2271479250571602 1102 74-75 -0.010710449927987753 1102 76-77 -0.510611911023922 1102 78-79 -0.5140197255285344 1102 80-81 -0.5117459506477111 1102 82-83 -0.564813559647817 1102 84-85 -0.7860269963151865 1102 86-87 -0.6444783630142545 1102 88-89 -0.5493002473029627 1102 90-91 -0.6120771838479797 1102 92-93 -0.3938188799743969 1102 94-95 -0.3014032949172929 1102 96-97 -0.6454850095207973 1102 98-99 -0.616395165293131 1102 100-101 -0.8008992475894505 1103 1 0.37308189260724944 1103 2 0.4385232734716382 1103 3 0.44803545635621234 1103 4 0.3342603694489341 1103 5 0.32441575542679857 1103 6 0.2778224570210739 1103 7 0.2930833431695987 1103 8 0.353382424023458 1103 9 0.501214096592193 1103 10-11 0.3928688414390962 1103 12-13 0.25594306199830186 1103 14-15 0.3825043379577906 1103 16-17 0.43307069947680077 1103 18-19 0.4579068521445038 1103 20-21 0.592762420143238 1103 22-23 0.5257569740635191 1103 24-25 0.5641083495153438 1103 26-27 0.5181454779011503 1103 28-29 0.512156431404442 1103 30-31 0.45381853577438136 1103 32-33 0.5059122237662521 1103 34-35 0.45332450753878106 1103 36-37 0.4449235477366855 1103 38-39 0.5027659121904051 1103 40-41 0.6958393896078761 1103 42-43 0.7213184797296108 1103 44-45 0.784999000684131 1103 46-47 0.6821509002504413 1103 48-49 0.4368682080168398 1103 50-51 0.5660019540971533 1103 52-53 0.3801136832445522 1103 54-55 0.38267504700726107 1103 56-57 0.3226734306137189 1103 58-59 0.4010494743742967 1103 60-61 0.33193059968687777 1103 62-63 0.21359138437638947 1103 64-65 0.13407645748620212 1103 66-67 0.020865839461269076 1103 68-69 0.11155515393016913 1103 70-71 -0.06868313488423894 1103 72-73 -0.0991678581888813 1103 74-75 0.047409795547903144 1103 76-77 0.03101785239004684 1103 78-79 -0.17939467498037942 1103 80-81 -0.0667642554880139 1103 82-83 -0.22045556046981574 1103 84-85 -0.48259790886130105 1103 86-87 -0.35247543225509403 1103 88-89 -0.3116222123670589 1103 90-91 -0.5877818188965165 1103 92-93 -0.852172577287508 1103 94-95 -0.9793294851040457 1103 96-97 -1.123665160243192 1103 98-99 -1.1358689187074553 1103 100-101 -1.3763530645642046 1104 1 0.3201781183886361 1104 2 0.2306377925092562 1104 3 0.32034337844674 1104 4 0.18433713682138375 1104 5 0.22998074304897642 1104 6 0.3272943082586366 1104 7 0.3995677459301987 1104 8 0.36467733447348394 1104 9 0.45460414529649995 1104 10-11 0.41249018730319875 1104 12-13 0.4215845838599037 1104 14-15 0.45198502263102824 1104 16-17 0.4290447996794242 1104 18-19 0.4768255965771715 1104 20-21 0.3919448405814663 1104 22-23 0.4837378218017818 1104 24-25 0.5879546549078114 1104 26-27 0.44391361917259076 1104 28-29 0.4405662772010359 1104 30-31 0.4842650674852109 1104 32-33 0.41931112127763726 1104 34-35 0.4623991462274404 1104 36-37 0.3896687166953541 1104 38-39 0.3936495401295801 1104 40-41 0.5329606221078578 1104 42-43 0.5207360827071525 1104 44-45 0.2972357941469781 1104 46-47 0.33776385947444254 1104 48-49 0.2593230679564087 1104 50-51 0.21450845530598883 1104 52-53 0.28679104841103964 1104 54-55 0.1948094156561524 1104 56-57 0.24083221264484678 1104 58-59 0.24224003089867807 1104 60-61 0.20482045765763957 1104 62-63 0.3571659693932112 1104 64-65 0.09182122601970377 1104 66-67 0.1004459233430417 1104 68-69 0.14681408913737926 1104 70-71 0.00938824532215321 1104 72-73 0.017534944095618954 1104 74-75 0.15606822775141183 1104 76-77 -0.0034769957545321972 1104 78-79 -0.13374991999934238 1104 80-81 -0.08776710662797171 1104 82-83 -0.29261868389889045 1104 84-85 -0.09208857470201082 1104 86-87 -0.1890289474270581 1104 88-89 -0.30420179334760533 1104 90-91 -0.24380865327549373 1104 92-93 -0.43035655305602205 1104 94-95 -0.6690781139104232 1104 96-97 -0.8989587477273808 1104 98-99 -0.9707406737712461 1104 100-101 -1.0979372879660954 1105 1 0.1553166767180194 1105 2 0.20460964425000583 1105 3 0.27626395097378165 1105 4 0.25995564207033084 1105 5 0.22348741778255032 1105 6 0.20891562042192646 1105 7 0.3614033213245804 1105 8 0.3332270800286139 1105 9 0.2226592468256925 1105 10-11 0.29727880354976577 1105 12-13 0.21274673521922693 1105 14-15 0.15183691093981366 1105 16-17 0.3976989005142926 1105 18-19 0.35278187317108234 1105 20-21 0.16932821202874493 1105 22-23 0.31954449660815953 1105 24-25 0.30053121345998335 1105 26-27 0.4017593039240239 1105 28-29 0.2514611625546266 1105 30-31 0.15269339688580885 1105 32-33 0.07481189435611668 1105 34-35 0.047613726164669856 1105 36-37 0.2264289075344692 1105 38-39 0.12981209070851918 1105 40-41 0.1925185100443585 1105 42-43 -0.02204824720696763 1105 44-45 0.1803952545962062 1105 46-47 0.3048676776885202 1105 48-49 0.32609867668139003 1105 50-51 0.14206574817470852 1105 52-53 0.0978815392424579 1105 54-55 0.08180939308628865 1105 56-57 0.345263331971708 1105 58-59 0.33938521020652956 1105 60-61 0.42307054581358017 1105 62-63 0.38222191474404 1105 64-65 0.23017679066791885 1105 66-67 0.2678283944226756 1105 68-69 0.15017202362119164 1105 70-71 0.06020319938520302 1105 72-73 -0.02533080720536418 1105 74-75 -0.09891030782366528 1105 76-77 -0.11468295691641117 1105 78-79 -0.2352163786349699 1105 80-81 -0.12117802689835244 1105 82-83 -0.28846061137488377 1105 84-85 -0.3928402482088025 1105 86-87 -0.3679072446595306 1105 88-89 -0.2134863553915487 1105 90-91 -0.45668759945219506 1105 92-93 -0.6430435340553515 1105 94-95 -0.7459551923703032 1105 96-97 -0.9484958349870816 1105 98-99 -1.0630747106533143 1105 100-101 -1.2365457321526456 1106 1 0.35639440239487996 1106 2 0.3647914671226644 1106 3 0.5114684022694611 1106 4 0.44739718978996734 1106 5 0.37156491556270765 1106 6 0.376894305629925 1106 7 0.41236027249664176 1106 8 0.2618212368430477 1106 9 0.5365474548910854 1106 10-11 0.5263072003394313 1106 12-13 0.6152245475398388 1106 14-15 0.7149208962512361 1106 16-17 0.24021757299447444 1106 18-19 0.565732533534046 1106 20-21 0.6768406065996473 1106 22-23 0.6713285945779432 1106 24-25 0.6256890211796602 1106 26-27 0.6336665091149101 1106 28-29 0.505615171279203 1106 30-31 0.5031341673916785 1106 32-33 0.5304705319638074 1106 34-35 0.649567134187933 1106 36-37 0.7338213069642947 1106 38-39 0.7348429105131729 1106 40-41 0.5971031575462078 1106 42-43 0.6436666550876993 1106 44-45 0.8462912917734684 1106 46-47 0.9356341631128018 1106 48-49 0.8589922607440954 1106 50-51 0.7915697503418784 1106 52-53 0.8382160851582725 1106 54-55 0.7683910876057993 1106 56-57 0.5784383469677437 1106 58-59 0.6712722863132754 1106 60-61 0.8822359171239569 1106 62-63 0.7494939857876197 1106 64-65 0.4712532102386575 1106 66-67 0.3098491340979841 1106 68-69 0.2079348358235542 1106 70-71 0.2809456159072816 1106 72-73 0.3022153472527016 1106 74-75 0.11105821125623194 1106 76-77 -0.188116489551863 1106 78-79 -0.2367534720489708 1106 80-81 -0.42853194188428034 1106 82-83 -0.12550586607095227 1106 84-85 -0.08580184116378575 1106 86-87 -0.0742057667708913 1106 88-89 -0.6528179945493662 1106 90-91 -0.3520302023765822 1106 92-93 -0.5713670635303494 1106 94-95 -0.8335572890200069 1106 96-97 -0.7493776716195448 1106 98-99 -1.0795175386794504 1106 100-101 -1.2303828462063482 1107 1 -0.07074939760193644 1107 2 -0.03630826410715571 1107 3 0.06715670838120147 1107 4 0.08344618866081532 1107 5 -0.0021051491647838816 1107 6 -0.04303126570549409 1107 7 0.012516922570590339 1107 8 0.04192270156099909 1107 9 0.10132067011397794 1107 10-11 0.01768250051914322 1107 12-13 0.022278174346880064 1107 14-15 -0.022040171831910982 1107 16-17 0.0055376719146167375 1107 18-19 0.09360849371152824 1107 20-21 -5.17891468071241E-4 1107 22-23 0.18424626184364712 1107 24-25 0.16499864760501737 1107 26-27 0.14290647794393152 1107 28-29 0.04971673937030374 1107 30-31 0.020639017977721608 1107 32-33 0.17661829194726408 1107 34-35 0.13760765646086526 1107 36-37 -0.04293941601157769 1107 38-39 0.003174746261201733 1107 40-41 0.028091643368178154 1107 42-43 -0.10362079382211675 1107 44-45 -0.1356124439729669 1107 46-47 -0.22322650579096148 1107 48-49 -0.23017602278458327 1107 50-51 -0.2769080005765474 1107 52-53 -0.2765698529132621 1107 54-55 -0.2287025366566695 1107 56-57 -0.38848721641589634 1107 58-59 -0.5347782233958469 1107 60-61 -0.4490620917502284 1107 62-63 -0.4581766991787468 1107 64-65 -0.5070284895339228 1107 66-67 -0.40677227368521685 1107 68-69 -0.5877481425953128 1107 70-71 -0.5631040668166776 1107 72-73 -0.5675822004182187 1107 74-75 -0.7057437019421293 1107 76-77 -0.7618135903327286 1107 78-79 -0.8838802949946292 1107 80-81 -0.9586711775591503 1107 82-83 -1.1769280419961419 1107 84-85 -1.1619659818148733 1107 86-87 -0.9746067352977406 1107 88-89 -0.9479284447791727 1107 90-91 -0.9615520618209104 1107 92-93 -1.0234605685161178 1107 94-95 -0.829820946202851 1107 96-97 -0.8210719177112438 1107 98-99 -0.9019045836388173 1107 100-101 -1.1384831261090174 1108 1 -0.13127635409843919 1108 2 -0.029295237727083645 1108 3 -0.05612490263970926 1108 4 0.08592440950140912 1108 5 0.09472887130915097 1108 6 0.11186438942677057 1108 7 0.059597475228351016 1108 8 0.015570845797647337 1108 9 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62-63 -0.2680209559635216 2316 64-65 -0.15127303326483243 2316 66-67 -0.1566305831184991 2316 68-69 -0.393027744101051 2316 70-71 -0.326667619128326 2316 72-73 -0.300513852817609 2316 74-75 -0.13519780583932928 2316 76-77 -0.266928456092284 2316 78-79 -0.10317272977331626 2316 80-81 -0.11048416201720457 2316 82-83 -0.2814034631390676 2316 84-85 -0.33686343349457104 2316 86-87 -0.2823822916196512 2316 88-89 -0.34108539063627674 2316 90-91 -0.37494011063085253 2316 92-93 -0.40677674983347956 2316 94-95 -0.10249771200562208 2316 96-97 -0.3166882407555498 2316 98-99 -0.36086824901189374 2316 100-101 -0.32866015019241246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8222.0 3 2569.0 4 2438.0 5 2706.0 6 3162.0 7 3816.0 8 4737.0 9 5896.0 10 7349.0 11 9227.0 12 11334.0 13 13743.0 14 16205.0 15 19619.0 16 22894.0 17 27976.0 18 27234.0 19 23640.0 20 24648.0 21 25992.0 22 28330.0 23 31490.0 24 35134.0 25 39390.0 26 44257.0 27 50041.0 28 56814.0 29 65051.0 30 75574.0 31 89664.0 32 107006.0 33 131894.0 34 166462.0 35 212396.0 36 261417.0 37 270388.0 38 137384.0 39 8415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.425444503985204 18.694898643918876 14.83549852515919 23.044158326936724 2 15.244615884719598 19.528902573243933 41.07607067377764 24.150410868258824 3 19.06906124025394 24.02046974195573 29.906824051946796 27.00364496584353 4 12.596975648511451 16.631423271645726 37.277070040636495 33.49453103920633 5 13.453472714592337 37.38792475575759 34.52574373914505 14.632858790505022 6 32.18135590436934 36.252440370907394 17.132442225637586 14.433761499085676 7 28.32259924186204 33.117814600456654 20.356115293750698 18.203470863930605 8 26.17247705359861 35.20501615305094 19.457274854641838 19.165231938708615 9 26.32558700740848 16.829894543787255 20.258452182726415 36.58606626607785 10-11 24.938463669075684 26.597375061102042 27.029709706792232 21.434451563030045 12-13 25.948287848474784 24.000126489876948 27.53793247951057 22.5136531821377 14-15 22.62323253167475 25.386329929029746 26.16868016561738 25.821757373678132 16-17 21.84861293933483 28.54534212039197 27.10021272559557 22.505832214677632 18-19 21.82629115912892 26.87836229928778 29.612030786533666 21.683315755049634 20-21 22.425825516495365 26.071642508602828 30.018733380219075 21.48379859468273 22-23 22.16111651810188 26.01558179354848 29.861256348518257 21.96204533983138 24-25 21.88709360300785 26.1759009802174 30.00955048933577 21.927454927438987 26-27 21.56480515969567 26.508926157648787 29.79516664735643 22.131102035299115 28-29 21.659790351213363 26.815096295767155 29.70606888735939 21.81904446566009 30-31 21.846251020249124 26.455955516534974 29.533868893252567 22.163924569963335 32-33 21.54072254324798 26.469345425191882 29.62096705105295 22.368964980507187 34-35 22.192422869144547 26.713727503425105 29.33644316669365 21.757406460736707 36-37 22.34494407799433 26.206786581613468 29.340652739820044 22.107616600572165 38-39 21.80378257204095 26.619028418967073 29.25686455702796 22.320324451964016 40-41 22.348155251018216 26.36528113538449 29.323965047876843 21.962598565720448 42-43 22.003994328545463 26.36987598094627 29.18435579574371 22.441773894764562 44-45 22.036019739895746 26.719314104448227 29.203100688465877 22.041565467190154 46-47 22.364520496907893 26.987697876337315 28.473574488556963 22.174207138197836 48-49 22.223042050689994 26.966521340014456 28.44064526287438 22.369791346421174 50-51 22.320122679827882 27.23527387490236 28.302838212962556 22.1417652323072 52-53 22.583328250370133 27.026649755065463 28.250852310465945 22.13916968409846 54-55 22.184788393567874 27.110306971059885 28.393683626192555 22.31122100917969 56-57 22.242741633344053 27.25841969567731 28.205540530310518 22.293298140668117 58-59 22.279044564744797 26.871236745639997 28.20832618934452 22.641392500270687 60-61 22.080071138767615 27.14873244286101 28.372804370559102 22.398392047812273 62-63 22.105538574692687 27.11295991511578 28.517793733760165 22.263707776431367 64-65 21.918860561256047 27.15968562717234 28.388459493574743 22.532994317996867 66-67 21.821902000336596 27.1951935211489 28.36623987739795 22.616664601116554 68-69 22.30204358059651 26.88881851660423 28.394847044392186 22.414290858407078 70-71 22.10302985580682 26.919203684384918 28.064521107611224 22.913245352197045 72-73 22.108815403413846 27.0451268574168 28.038586666750266 22.807471072419087 74-75 22.084373408540255 27.350971851417462 27.9357850815599 22.628869658482383 76-77 22.23246525258204 26.985317055272347 28.01890417682599 22.763313515319624 78-79 22.251534643046476 27.123530598719924 27.954743850880647 22.670190907352954 80-81 22.379894477104763 27.168179675813033 27.678990528976605 22.772935318105603 82-83 22.40913618747583 27.125370894843776 27.809567616408188 22.6559253012722 84-85 22.41907461429405 27.233700233813778 27.63141749391841 22.715807657973762 86-87 22.427595524341193 27.113117136473264 27.562351312022233 22.89693602716331 88-89 22.45513220783086 27.216823350908303 27.358903989328155 22.96914045193268 90-91 22.370170371603606 27.20919683337073 27.362557818008092 23.05807497701757 92-93 22.520087967096043 26.801524782295427 27.54978060087692 23.12860664973161 94-95 22.32241902185829 26.7505917375992 27.62638509291601 23.3006041476265 96-97 22.590281752339937 26.37412120471019 27.653425736929048 23.382171306020822 98-99 22.32893811550011 26.54537614659429 27.301629179178843 23.824056558726753 100-101 23.176975796145754 25.492472301336743 27.418557229503758 23.91199467301374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 839.0 1 554.0 2 383.0 3 504.0 4 412.0 5 370.0 6 716.0 7 1571.0 8 2673.0 9 3176.5 10 2713.5 11 2111.5 12 1982.5 13 2156.0 14 2334.0 15 2467.5 16 2672.0 17 2958.0 18 3223.0 19 3603.0 20 4158.0 21 4898.0 22 6017.5 23 7333.5 24 9033.5 25 11238.5 26 14027.5 27 17239.0 28 20002.5 29 22649.5 30 25801.0 31 29996.0 32 35180.5 33 38377.0 34 40237.5 35 43050.0 36 46657.0 37 50619.0 38 53966.5 39 56464.0 40 59191.0 41 62199.5 42 64289.0 43 66460.0 44 69357.0 45 72565.5 46 74220.0 47 75932.0 48 76862.5 49 75155.0 50 73041.0 51 71345.5 52 69909.0 53 69139.0 54 69131.0 55 66079.5 56 60815.0 57 55567.0 58 51208.5 59 45738.5 60 37553.5 61 30751.5 62 25721.0 63 20190.0 64 14873.5 65 11245.0 66 8487.5 67 6358.0 68 4750.0 69 3475.5 70 2630.5 71 1868.5 72 1317.0 73 962.0 74 695.0 75 490.5 76 334.0 77 226.5 78 142.0 79 88.5 80 63.0 81 42.5 82 26.0 83 19.0 84 11.5 85 9.5 86 8.0 87 5.5 88 4.0 89 2.0 90 3.0 91 2.5 92 2.0 93 1.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1496254062397265 2 0.05302446741742886 3 0.1525176499170408 4 0.13164529137908926 5 0.19426236699294389 6 0.19917918124437822 7 0.1632671555843923 8 0.07380041783280325 9 0.12359521314389779 10-11 0.10416897644460342 12-13 0.15434940424600652 14-15 0.1384179619901336 16-17 0.13646569750794643 18-19 0.1363692893853693 20-21 0.13550161628217502 22-23 0.1999986502862839 24-25 0.2151347255308954 26-27 0.14817928440106937 28-29 0.12860843551790926 30-31 0.10139724292051054 32-33 0.12516184513577638 34-35 0.09392561342078193 36-37 0.0892016154145019 38-39 0.05350650803031457 40-41 0.06160479032679461 42-43 0.0638462791767132 44-45 0.040756533819487364 46-47 0.052614732896475995 48-49 0.043841593741955946 50-51 0.05541056845121315 52-53 0.028560906313478726 54-55 0.051939876038435986 56-57 0.028777824589277298 58-59 0.05266293695776456 60-61 0.03955143228727307 62-63 0.053096773509361705 64-65 0.0415277988001045 66-67 0.03918990182760878 68-69 0.0680882365701075 70-71 0.05374752833675742 72-73 0.053193181631938855 74-75 0.045601041978988816 76-77 0.02289692911207155 78-79 0.023981520491064414 80-81 0.026825560107090144 82-83 0.02294513317336012 84-85 0.020607236200864396 86-87 0.026030193095828706 88-89 0.06726876752820178 90-91 0.10985705567665488 92-93 0.08163357779219615 94-95 0.07681317166333898 96-97 0.06914872591845608 98-99 0.057844873546286014 100-101 0.13489906551606784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2074514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.33191328457771 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4699058944753 45.189678328009286 2 12.977938011761129 15.140559094293495 3 4.260400785293907 7.455519874959328 4 1.838174071547962 4.288968421739794 5 0.991618351345706 2.8921497841046815 6 0.5737596863417749 2.008110016192495 7 0.371863299723223 1.5184048427220382 8 0.26404123449006883 1.2321624315062034 9 0.19357171397759723 1.016226759067945 >10 0.9633920786722644 10.144486636470656 >50 0.06034486018521328 2.398910448044508 >100 0.030194831329603416 3.1711607440970964 >500 0.0032243457480915626 1.3404477747596235 >1k 0.0014054840440398955 1.3823081334029412 >5k 1.6535106400469358E-4 0.8209067106299015 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9405 0.4533591964190167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7617 0.3671703348350505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4757 0.2293067195497355 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2101 0.10127673276728912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.8204061288571685E-5 14-15 0.0 0.0 0.0 0.0 4.8204061288571685E-5 16-17 2.4102030644285843E-5 0.0 0.0 0.0 4.8204061288571685E-5 18-19 4.8204061288571685E-5 0.0 0.0 0.0 4.8204061288571685E-5 20-21 4.8204061288571685E-5 0.0 0.0 0.0 4.8204061288571685E-5 22-23 4.8204061288571685E-5 0.0 0.0 9.640812257714337E-5 4.8204061288571685E-5 24-25 1.4461218386571504E-4 0.0 0.0 2.6512233708714424E-4 4.8204061288571685E-5 26-27 1.4461218386571504E-4 0.0 0.0 8.917751338385761E-4 4.8204061288571685E-5 28-29 2.6512233708714424E-4 0.0 0.0 0.003446590382132875 4.8204061288571685E-5 30-31 2.892243677314301E-4 0.0 0.0 0.012292035628585779 4.8204061288571685E-5 32-33 2.892243677314301E-4 0.0 0.0 0.03253774136978589 4.8204061288571685E-5 34-35 3.1332639837571593E-4 0.0 0.0 0.06182170860259319 4.8204061288571685E-5 36-37 4.338365515971452E-4 0.0 0.0 0.10920630084925914 4.8204061288571685E-5 38-39 4.820406128857168E-4 0.0 0.0 0.17842733285964807 4.8204061288571685E-5 40-41 5.061426435300027E-4 0.0 0.0 0.2542523212665714 4.8204061288571685E-5 42-43 6.025507661071461E-4 0.0 0.0 0.32971577921383033 4.8204061288571685E-5 44-45 6.748568580400036E-4 0.0 0.0 0.42277371953141796 4.8204061288571685E-5 46-47 7.230609193285753E-4 0.0 0.0 0.5294734091936715 4.8204061288571685E-5 48-49 7.230609193285753E-4 0.0 0.0 0.6381494653687563 4.8204061288571685E-5 50-51 7.230609193285753E-4 0.0 0.0 0.759888822153044 4.8204061288571685E-5 52-53 7.230609193285753E-4 0.0 0.0 0.8711437956070676 4.8204061288571685E-5 54-55 7.230609193285753E-4 0.0 0.0 0.995558477792871 4.8204061288571685E-5 56-57 7.230609193285753E-4 0.0 0.0 1.1363625408167888 4.8204061288571685E-5 58-59 7.230609193285753E-4 0.0 0.0 1.2808542145292825 9.640812257714337E-5 60-61 7.230609193285753E-4 0.0 0.0 1.435203618775289 9.640812257714337E-5 62-63 7.230609193285753E-4 0.0 0.0 1.585142351413391 9.640812257714337E-5 64-65 7.71264980617147E-4 0.0 0.0 1.7403594287625923 9.640812257714337E-5 66-67 8.194690419057187E-4 0.0 0.0 1.921052352502803 9.640812257714337E-5 68-69 8.194690419057187E-4 0.0 0.0 2.115001393097371 9.640812257714337E-5 70-71 8.194690419057187E-4 0.0 0.0 2.323074223649491 9.640812257714337E-5 72-73 8.676731031942904E-4 0.0 0.0 2.5255794851227806 9.640812257714337E-5 74-75 9.158771644828621E-4 0.0 0.0 2.729410358281506 9.640812257714337E-5 76-77 9.158771644828621E-4 0.0 0.0 2.951606978791177 9.640812257714337E-5 78-79 9.158771644828621E-4 0.0 0.0 3.1984358746193084 9.640812257714337E-5 80-81 9.399791951271478E-4 0.0 0.0 3.4563999086051 9.640812257714337E-5 82-83 0.0010122852870600055 0.0 0.0 3.7178828390649565 9.640812257714337E-5 84-85 0.0010363873177042914 0.0 0.0 3.978449892360331 9.640812257714337E-5 86-87 0.0010604893483485772 4.8204061288571685E-5 0.0 4.250561818334319 9.640812257714337E-5 88-89 0.0010604893483485772 4.8204061288571685E-5 0.0 4.537496493154541 9.640812257714337E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4945 0.0 49.493225 1 GTATCAA 8525 0.0 38.595135 1 ATCAACG 10480 0.0 30.952757 3 TATCAAC 10555 0.0 30.777138 2 TCAACGC 10625 0.0 30.440811 4 CAACGCA 10780 0.0 30.003117 5 AACGCAG 11185 0.0 28.991966 6 ACGCAGA 12395 0.0 25.85114 7 CGCAGAG 12430 0.0 25.623505 8 GTGGTAT 2445 0.0 23.759094 1 GCAGAGT 13355 0.0 23.313704 9 TGGTATC 2400 0.0 22.790422 2 GAGTACT 8765 0.0 22.153828 12-13 GTGTAGC 1295 0.0 22.061264 1 GTACATG 7130 0.0 21.77093 1 TACATGG 6935 0.0 21.603785 2 GTACTTT 8915 0.0 21.434757 14-15 CAGAGTA 12905 0.0 21.179634 10-11 TAAGGTG 1260 0.0 21.139452 5 AGAGTAC 12020 0.0 20.702408 10-11 >>END_MODULE