FastQCFastQC Report
Thu 26 May 2016
SRR938080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074514
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93090.44873160653531374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80750.3892477949052163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.23750140996879268No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21580.1040243642607377No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21400.1031566911575434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.051.434141
GTATCAA84850.040.69861
ATCAACG104400.032.7988363
TATCAAC107400.032.1510852
TCAACGC107100.031.9253184
CAACGCA108400.031.542455
AACGCAG111900.030.5983096
ACGCAGA125550.027.157487
CGCAGAG126450.026.813978
GCAGAGT139050.024.0085589
TGGTATC22550.023.1692312
GAGTACT89100.022.41307612-13
GTGGTAT23800.021.9539491
CAGAGTA133150.021.4888610-11
GTACTTT93650.021.29826414-15
TACATGG67150.021.0075762
AGAGTAC124800.020.96691310-11
GTACATG70650.020.707871
TGCACCG4250.020.1129515
AGTACTT92700.019.92888612-13
Produced by FastQC (version 0.11.5)