##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938080_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2074514 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.499833695988556 34.0 31.0 34.0 31.0 34.0 2 32.71257316171402 34.0 31.0 34.0 31.0 34.0 3 32.76876222575505 34.0 31.0 34.0 31.0 34.0 4 36.232911901293505 37.0 35.0 37.0 35.0 37.0 5 36.2081880382586 37.0 37.0 37.0 35.0 37.0 6 36.2363681324879 37.0 37.0 37.0 35.0 37.0 7 36.19274393906235 37.0 37.0 37.0 35.0 37.0 8 36.17977993881941 37.0 37.0 37.0 35.0 37.0 9 37.86191416399215 39.0 38.0 39.0 35.0 39.0 10-11 37.8393399610704 39.0 38.0 39.0 35.0 39.0 12-13 37.763786602548834 39.0 38.0 39.0 35.0 39.0 14-15 39.03626150510433 40.0 38.0 41.0 36.0 41.0 16-17 38.92399328228202 40.0 38.0 41.0 35.5 41.0 18-19 39.179675818047016 40.0 39.0 41.0 36.0 41.0 20-21 39.16402636954969 40.0 39.0 41.0 36.0 41.0 22-23 39.07053194145713 40.0 39.0 41.0 35.5 41.0 24-25 38.980609916346665 40.0 38.5 41.0 35.0 41.0 26-27 38.810042978741045 40.0 38.0 41.0 35.0 41.0 28-29 38.69137084637654 40.0 38.0 41.0 34.5 41.0 30-31 38.45051154149839 40.0 38.0 41.0 34.0 41.0 32-33 38.23288370191766 40.0 38.0 41.0 34.0 41.0 34-35 38.0872527252166 40.0 38.0 41.0 34.0 41.0 36-37 38.07680136166832 40.0 38.0 41.0 33.5 41.0 38-39 37.928128949720275 40.0 38.0 41.0 33.0 41.0 40-41 37.75463915885841 40.0 37.0 41.0 33.0 41.0 42-43 37.51776054536147 40.0 37.0 41.0 33.0 41.0 44-45 37.24441049807328 40.0 37.0 41.0 32.0 41.0 46-47 36.84321701371984 40.0 36.0 41.0 31.0 41.0 48-49 36.4429138583784 39.0 36.0 41.0 30.0 41.0 50-51 36.067024131917165 39.0 35.0 41.0 29.5 41.0 52-53 35.551819606905525 39.0 35.0 40.0 28.0 41.0 54-55 35.2436107926965 38.0 35.0 40.0 27.0 41.0 56-57 34.90447762704903 38.0 34.0 40.0 26.0 41.0 58-59 34.4711915658318 38.0 34.0 40.0 25.5 41.0 60-61 34.00563240354126 37.0 33.0 40.0 24.5 41.0 62-63 33.541512373500495 37.0 33.0 39.5 23.0 41.0 64-65 33.05546961842629 36.0 32.0 39.0 22.0 40.5 66-67 32.511681531192366 35.5 31.5 39.0 20.0 40.0 68-69 31.94119345543101 35.0 31.0 38.0 18.0 40.0 70-71 31.338839602914224 35.0 30.5 37.5 13.5 40.0 72-73 30.75305228116079 34.5 30.0 37.0 8.0 39.0 74-75 30.162741249275733 34.0 29.0 36.0 7.0 39.0 76-77 28.81426396736778 32.5 28.0 35.0 6.0 37.0 78-79 29.48725291803285 34.0 29.0 35.0 2.0 37.5 80-81 29.16820011819636 34.0 29.0 35.0 2.0 37.0 82-83 28.605509049348427 34.0 28.5 35.0 2.0 36.5 84-85 28.00831568261289 33.0 26.5 35.0 2.0 36.0 86-87 27.367320249465656 33.0 26.0 35.0 2.0 36.0 88-89 26.62374006634807 32.0 25.0 35.0 2.0 35.0 90-91 26.08186495728638 32.0 24.0 34.5 2.0 35.0 92-93 25.277012350844586 31.5 20.5 34.0 2.0 35.0 94-95 24.458117660329115 31.0 16.0 34.0 2.0 35.0 96-97 23.424143437932933 31.0 2.0 34.0 2.0 35.0 98-99 21.99540687602012 30.0 2.0 34.0 2.0 35.0 100-101 17.643565914715445 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10763544193751784 1101 2 0.09111333063621885 1101 3 0.13322362556458955 1101 4 0.07690476467454488 1101 5 0.02723906335034343 1101 6 0.09585112400073115 1101 7 0.08885600425850271 1101 8 0.10940367394598383 1101 9 -0.020551870304856834 1101 10-11 0.0012136644965536902 1101 12-13 0.14307933924849436 1101 14-15 0.14435810146238026 1101 16-17 0.21445000691637262 1101 18-19 0.15911016244502463 1101 20-21 0.23052433547731965 1101 22-23 0.21210797243785606 1101 24-25 0.2758759204501615 1101 26-27 0.18673222816099866 1101 28-29 0.2672520017124711 1101 30-31 0.1988428185583615 1101 32-33 0.14735940854497187 1101 34-35 0.10347263866612622 1101 36-37 0.29503651570647804 1101 38-39 0.24718613191446792 1101 40-41 0.2592389543371709 1101 42-43 0.1918121870725571 1101 44-45 0.2934809168698891 1101 46-47 0.22179120996666057 1101 48-49 0.14838356758094307 1101 50-51 0.2507861149591548 1101 52-53 -0.026800484395337776 1101 54-55 -0.08232561520070902 1101 56-57 0.07681451894619329 1101 58-59 0.123471480442916 1101 60-61 0.04087256276199014 1101 62-63 -0.010391264905400988 1101 64-65 0.004720077005792689 1101 66-67 -0.20430861910514153 1101 68-69 0.007416833219846097 1101 70-71 -0.202797824328119 1101 72-73 -0.16127352932617 1101 74-75 -0.3478538706330916 1101 76-77 -0.31239941458024845 1101 78-79 -0.19302165475491506 1101 80-81 -0.18853036967547254 1101 82-83 -0.19238643363675223 1101 84-85 -0.20779173141334795 1101 86-87 -0.29724530211352373 1101 88-89 -0.30914118759324083 1101 90-91 -0.6621707101701411 1101 92-93 -0.9834786415053252 1101 94-95 -0.9413301310286926 1101 96-97 -0.7800555833247849 1101 98-99 -0.5506582806200448 1101 100-101 -1.2591009680603555 1102 1 0.13545780683853792 1102 2 0.10127919473466562 1102 3 0.09041998725533062 1102 4 0.0490823997735248 1102 5 0.055061428251356404 1102 6 0.008103665466755672 1102 7 0.06852427606160205 1102 8 0.03824262525684219 1102 9 0.10197354435538841 1102 10-11 0.10608257835423984 1102 12-13 0.155117862522971 1102 14-15 0.15987442034948174 1102 16-17 0.20481918829679557 1102 18-19 0.22866607469756417 1102 20-21 0.17514712841472857 1102 22-23 0.20247715381827192 1102 24-25 0.15816591509970834 1102 26-27 0.14606877176721156 1102 28-29 0.13777099582696906 1102 30-31 0.14159295231973346 1102 32-33 0.17651938714315207 1102 34-35 0.18158927858051044 1102 36-37 0.13372030382846845 1102 38-39 0.09309303400114999 1102 40-41 0.13671353967692568 1102 42-43 -0.01471536776959681 1102 44-45 0.00830167663447412 1102 46-47 0.1225402736370711 1102 48-49 0.10638249748998163 1102 50-51 0.16972672491100127 1102 52-53 0.05372386017395314 1102 54-55 0.16994832808448024 1102 56-57 0.11774549807942236 1102 58-59 0.17216061901969226 1102 60-61 0.15269706784491888 1102 62-63 0.2953872262663495 1102 64-65 0.17539958476395157 1102 66-67 0.0404746874759212 1102 68-69 0.2554104126740988 1102 70-71 0.2281813088982041 1102 72-73 0.1348741432259999 1102 74-75 0.13315201486717498 1102 76-77 -0.04674933432342243 1102 78-79 -0.037858465883861214 1102 80-81 -0.22946134880870162 1102 82-83 -0.04712158612471384 1102 84-85 -0.411109013382319 1102 86-87 -0.24267066326922304 1102 88-89 -0.4089271694016965 1102 90-91 -0.5433906138619555 1102 92-93 -0.6916113327840812 1102 94-95 -0.5807094782732101 1102 96-97 -0.5759357331375199 1102 98-99 -0.32165881566552557 1102 100-101 -0.7564257406660282 1103 1 0.06775015833446929 1103 2 0.11706262287938074 1103 3 0.11105521111025496 1103 4 0.06053832715950591 1103 5 0.07020395957054149 1103 6 0.019264366831166058 1103 7 0.005455893610765372 1103 8 0.03335444314621583 1103 9 0.13344199197096174 1103 10-11 0.13813651623107148 1103 12-13 0.16439598114759946 1103 14-15 0.18820784878363384 1103 16-17 0.14917942027663855 1103 18-19 0.15414548619715873 1103 20-21 0.17051861288889114 1103 22-23 0.19268094246952217 1103 24-25 0.1730281057429437 1103 26-27 0.28384670391153577 1103 28-29 0.2302681200608916 1103 30-31 0.25468271178081636 1103 32-33 0.15032862151539916 1103 34-35 0.17612304888144337 1103 36-37 0.2926358402425677 1103 38-39 0.19289142026428863 1103 40-41 0.09732650167050139 1103 42-43 0.12389242239333242 1103 44-45 0.12493869665443214 1103 46-47 0.09107472039425346 1103 48-49 0.19485396269589472 1103 50-51 -0.04836591651839228 1103 52-53 -0.051592441016126145 1103 54-55 -0.07892844854355019 1103 56-57 -0.02498677326719445 1103 58-59 0.16421432534671254 1103 60-61 8.516571210250845E-4 1103 62-63 -0.1178134290196482 1103 64-65 -0.016477482128316012 1103 66-67 0.012600223865213422 1103 68-69 -0.10573660244196859 1103 70-71 -0.5113813761865948 1103 72-73 -0.5259814499012698 1103 74-75 -0.6988135205959161 1103 76-77 -0.5003302109545089 1103 78-79 -0.48009629566662326 1103 80-81 -0.47243608589628394 1103 82-83 -0.47999322565456737 1103 84-85 -0.6860839357097568 1103 86-87 -0.9475207223557724 1103 88-89 -1.2145146319712552 1103 90-91 -1.3501966073636744 1103 92-93 -1.0635241741236676 1103 94-95 -1.1045243773464328 1103 96-97 -1.1707971464349285 1103 98-99 -1.5338853904730705 1103 100-101 -1.8858757354148068 1104 1 0.10435306696349045 1104 2 0.09925049171310718 1104 3 0.07741872799613247 1104 4 0.10927568129444865 1104 5 0.09749205126406224 1104 6 0.061726343732551925 1104 7 0.07882157350990582 1104 8 0.11207757104325111 1104 9 0.1994885760470524 1104 10-11 0.1174421180075953 1104 12-13 0.18886457236575893 1104 14-15 0.1618173192104564 1104 16-17 0.02793411079768049 1104 18-19 0.16553749270643436 1104 20-21 0.17875422890190862 1104 22-23 0.041887816255659516 1104 24-25 0.1139117208726077 1104 26-27 0.08455038134152204 1104 28-29 0.10955978509434772 1104 30-31 -0.057948281435010074 1104 32-33 -0.0720354446421112 1104 34-35 -0.09620654689408781 1104 36-37 -0.2176691515452518 1104 38-39 -0.139146103391667 1104 40-41 -0.18929158622372455 1104 42-43 -0.21680970764826668 1104 44-45 -0.06812016482125927 1104 46-47 -0.06328213862251886 1104 48-49 -0.16831719459946015 1104 50-51 -0.20751186572971392 1104 52-53 -0.2675472705971771 1104 54-55 -0.3073081151428383 1104 56-57 -0.23332468599910072 1104 58-59 -0.2037729502019161 1104 60-61 -0.27948106212456736 1104 62-63 -0.3529579240957972 1104 64-65 -0.4602815366445512 1104 66-67 -0.6619454421535487 1104 68-69 -0.5166147171695457 1104 70-71 -0.644420398298621 1104 72-73 -0.5981521309391802 1104 74-75 -0.44792293965661756 1104 76-77 -0.4906541659357515 1104 78-79 -0.7473484592563189 1104 80-81 -0.7907505290503849 1104 82-83 -0.8572695710306313 1104 84-85 -0.8833598148197837 1104 86-87 -0.8967826514008408 1104 88-89 -0.929947858447683 1104 90-91 -0.8963692421075145 1104 92-93 -1.1197383091066584 1104 94-95 -1.1376733306432278 1104 96-97 -1.2026892309540997 1104 98-99 -1.0302015044991926 1104 100-101 -1.3315836668818974 1105 1 0.11932732154523507 1105 2 0.0927819204897844 1105 3 0.08633625067945161 1105 4 0.07596863093041151 1105 5 0.12630551474541818 1105 6 0.12433465783902875 1105 7 0.06777301893973942 1105 8 0.07376243799875937 1105 9 0.0981671227129155 1105 10-11 0.04534159911916902 1105 12-13 0.10717841431701913 1105 14-15 0.06030778368609191 1105 16-17 -0.08336625548935217 1105 18-19 0.01472700407861538 1105 20-21 -0.010289607353499264 1105 22-23 0.03847709741523886 1105 24-25 0.08623755009058698 1105 26-27 0.07557946758485201 1105 28-29 -0.04934843055413296 1105 30-31 0.03996682141924879 1105 32-33 0.10424477015502731 1105 34-35 0.1296005623214782 1105 36-37 0.01824331039237137 1105 38-39 0.12653455149352766 1105 40-41 0.04380206972759737 1105 42-43 0.029376531579174525 1105 44-45 -0.06039957292781395 1105 46-47 -0.16220184305924334 1105 48-49 -0.07587334198697704 1105 50-51 -0.06687383269569125 1105 52-53 -0.018529605675183802 1105 54-55 0.07969720139463021 1105 56-57 0.06791223955845282 1105 58-59 -0.030162167729038458 1105 60-61 0.006611242789908545 1105 62-63 0.1313773776693381 1105 64-65 0.14339203615745788 1105 66-67 0.15210922399570848 1105 68-69 0.07326502914519395 1105 70-71 -0.025224220322872526 1105 72-73 -0.10026581072719054 1105 74-75 -0.03564386645770057 1105 76-77 -0.09348003410494243 1105 78-79 -0.2664866108428008 1105 80-81 -0.2989024965478393 1105 82-83 -0.1857304835471716 1105 84-85 -0.36965748121731323 1105 86-87 -0.4106964864597984 1105 88-89 -0.8616422314411203 1105 90-91 -0.9034570284383783 1105 92-93 -0.6895161949609125 1105 94-95 -0.8179285108514485 1105 96-97 -1.2279928380792882 1105 98-99 -1.6194037908991064 1105 100-101 -2.0821485808409683 1106 1 0.15647690348377807 1106 2 0.19472565365283856 1106 3 0.18036272324692249 1106 4 0.1742391421715226 1106 5 0.13294354382589546 1106 6 0.13558060783505255 1106 7 0.1700067291626297 1106 8 0.13798765369439536 1106 9 0.15289243193219448 1106 10-11 0.14360028553115 1106 12-13 0.09010954221222534 1106 14-15 0.21138610060442176 1106 16-17 0.24218634027564434 1106 18-19 0.33091809761730673 1106 20-21 0.3894241511576908 1106 22-23 0.3409512547886706 1106 24-25 0.2797019670297587 1106 26-27 0.3959109981692279 1106 28-29 0.3210725387378517 1106 30-31 0.22667858905920468 1106 32-33 0.2913116857880951 1106 34-35 0.34137518967811076 1106 36-37 0.16858311298221906 1106 38-39 0.21358786857310008 1106 40-41 0.16689558477630584 1106 42-43 0.11983324947959773 1106 44-45 0.23741251118756423 1106 46-47 0.1150792471039992 1106 48-49 0.20193113123838913 1106 50-51 0.12511436182489888 1106 52-53 0.021198192133141447 1106 54-55 -0.04495315990764226 1106 56-57 -0.15277199342938275 1106 58-59 -6.66878310973118E-4 1106 60-61 -0.16840859067350067 1106 62-63 -0.2414214572625042 1106 64-65 -0.2262694566089678 1106 66-67 -0.08438359618303792 1106 68-69 -0.4621558433981967 1106 70-71 -0.7942425767576218 1106 72-73 -0.7944332675074399 1106 74-75 -0.5184533867351284 1106 76-77 -0.5739403102039518 1106 78-79 -0.4588131567242115 1106 80-81 -0.4652279900194607 1106 82-83 -0.3971750906726008 1106 84-85 -0.694263418786516 1106 86-87 -0.9746744557281417 1106 88-89 -0.9732305908643255 1106 90-91 -1.1076569121250586 1106 92-93 -1.200415865585672 1106 94-95 -1.4941340521813729 1106 96-97 -1.543697769893022 1106 98-99 -1.3982933265703146 1106 100-101 -2.008969416404895 1107 1 0.005619701556263124 1107 2 0.02812790241633678 1107 3 0.004061811972889018 1107 4 0.05762429460715168 1107 5 0.022344218136872485 1107 6 0.005678335216209973 1107 7 0.06420431500209389 1107 8 0.016373124201237488 1107 9 0.07581184858958778 1107 10-11 0.10292494082147385 1107 12-13 0.015925394881634247 1107 14-15 0.06699767203526363 1107 16-17 0.002121095917424043 1107 18-19 0.13603089362234044 1107 20-21 0.008076642450497218 1107 22-23 0.014400458815444495 1107 24-25 0.08562584463594902 1107 26-27 0.07327844198990618 1107 28-29 0.11439185043174405 1107 30-31 0.19298689548901393 1107 32-33 0.11208153740787452 1107 34-35 0.20174788962562928 1107 36-37 0.026171695815918383 1107 38-39 0.016318112374683835 1107 40-41 0.027722530179381977 1107 42-43 0.05016372547318326 1107 44-45 0.07956171839506254 1107 46-47 0.06088388776304754 1107 48-49 -0.04818516355001634 1107 50-51 -0.1063480501091334 1107 52-53 -0.22871930814517327 1107 54-55 -0.12005573787484991 1107 56-57 -0.10773609114085758 1107 58-59 -0.12606844322135657 1107 60-61 -0.0478267986602674 1107 62-63 -0.1499710127579732 1107 64-65 -0.3464139007000071 1107 66-67 -0.17640259298642036 1107 68-69 0.020015270964307774 1107 70-71 -0.2241128434603361 1107 72-73 -0.290774454549215 1107 74-75 -0.42957832510883165 1107 76-77 -0.5630165518744619 1107 78-79 -0.2673392544674975 1107 80-81 -0.13276902120634304 1107 82-83 -0.3339981953657407 1107 84-85 -0.43140042001108725 1107 86-87 -0.5764085402396653 1107 88-89 -0.5173927912809972 1107 90-91 -0.6369721445556031 1107 92-93 -0.8231317166647294 1107 94-95 -0.8795836856042989 1107 96-97 -0.9290947227707704 1107 98-99 -1.2300575085706669 1107 100-101 -1.7842133372687758 1108 1 -0.09703438912049478 1108 2 -0.11179221244928073 1108 3 -0.11747541051716581 1108 4 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-0.464273281452229 2316 48-49 -0.22981400847542233 2316 50-51 -0.100406868246111 2316 52-53 0.07405397094196076 2316 54-55 -0.040790688117631646 2316 56-57 -0.03173792747970339 2316 58-59 0.04575919775256665 2316 60-61 0.23805720132809682 2316 62-63 0.10587049461138776 2316 64-65 0.3700050712121552 2316 66-67 0.2470902304500342 2316 68-69 0.5372991555630726 2316 70-71 0.6743018409934969 2316 72-73 0.7406274672041242 2316 74-75 1.027979347862793 2316 76-77 0.8242083065077281 2316 78-79 0.743979166251993 2316 80-81 0.909205035778367 2316 82-83 1.0890199919553716 2316 84-85 1.0927508189355954 2316 86-87 1.3631460639216364 2316 88-89 1.5672982432529352 2316 90-91 1.6612290218542398 2316 92-93 1.6608658889647714 2316 94-95 2.0176741926822324 2316 96-97 2.2891102050779892 2316 98-99 2.413460029774914 2316 100-101 3.032981458704082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 49.0 6 69.0 7 188.0 8 464.0 9 922.0 10 1608.0 11 2748.0 12 4223.0 13 6456.0 14 8649.0 15 11674.0 16 15634.0 17 22030.0 18 22131.0 19 17615.0 20 16100.0 21 16867.0 22 17903.0 23 19659.0 24 22346.0 25 25497.0 26 29176.0 27 34520.0 28 41377.0 29 49695.0 30 59434.0 31 73048.0 32 89925.0 33 116128.0 34 156332.0 35 221603.0 36 316336.0 37 402468.0 38 237505.0 39 14116.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60683731841055 19.173734839624707 15.329153332992659 22.890274508972087 2 15.473030924077563 19.829920707122568 40.58265102504136 24.11439734375851 3 19.070304688682622 23.932297617429185 30.153776139967235 26.84362155392096 4 12.820303936247237 16.919866532595105 37.04997893482522 33.209850596332444 5 13.397111196379644 37.493902121579644 34.62018653241372 14.488800149626993 6 32.27595788044788 36.46038806908347 16.9045098617675 14.359144188701148 7 28.36153431598919 33.06711837085698 20.325435258571407 18.245912054582423 8 26.201076493096696 35.48831196125936 19.2608485650133 19.049762980630643 9 26.104716574580845 16.84621072694617 20.18800547983769 36.861067218635306 10-11 24.948132430053498 26.697361406093183 26.98651346773268 21.367992696120634 12-13 25.912159996220772 23.96402697711592 27.541493150876427 22.582319875786876 14-15 22.517683659883716 25.43863285569536 26.14761818912767 25.89606529529326 16-17 21.825156276174074 28.624757616768765 27.099511798896337 22.450574308160828 18-19 21.75577026715655 26.891575569024837 29.62949394412378 21.72316021969483 20-21 22.30758625875747 26.075721831715764 30.064463291161207 21.552228618365554 22-23 22.140510982331282 26.022962486635425 29.91681425143431 21.91971227959898 24-25 21.90130003854055 26.16141669179395 30.06362926642559 21.873654003239913 26-27 21.485911245402225 26.54670625972409 29.85100532712727 22.11637716774641 28-29 21.62102749775293 26.79349074501915 29.751064118518904 21.834417638709013 30-31 21.798386795488426 26.47272700978697 29.60315843895408 22.125727755770527 32-33 21.49281711282739 26.46022152658406 29.67786671962686 22.36909464096169 34-35 22.197253048024297 26.650631009303943 29.417203726205333 21.73491221646643 36-37 22.28976469958742 26.222886800993205 29.440727077455403 22.046621421963973 38-39 21.777706326580393 26.582536898865918 29.364787071682475 22.274969702871214 40-41 22.318731454604922 26.366487255422527 29.364939149532375 21.949842140440175 42-43 21.965032168775377 26.37538296304634 29.27612843793016 22.38345643024812 44-45 22.021519257040445 26.660075564686476 29.321036155938213 21.99736902233487 46-47 22.354556015637428 26.90249745723086 28.577664123094127 22.16528240403758 48-49 22.13733172170195 26.948099604603996 28.528695434206515 22.385873239487537 50-51 22.323390819142656 27.21761451968618 28.39685696036251 22.062137700808655 52-53 22.608570101470853 26.998931467100736 28.26921533336274 22.123283098065674 54-55 22.216975583606192 27.150149637668587 28.403943608196137 22.228931170529094 56-57 22.270488137954807 27.273097631206888 28.22235052063308 22.23406371020523 58-59 22.264866355643072 26.971107206708545 28.176428101669615 22.58759833597877 60-61 21.98633219189886 27.253642133658946 28.378766956713303 22.381258717728887 62-63 22.030308392303283 27.27912003351849 28.459860979258742 22.230710594919483 64-65 21.87086107882073 27.262264706775742 28.317248142653472 22.54962607175006 66-67 21.740439459673933 27.319620929029533 28.24555533646478 22.69438427483176 68-69 22.212240774695342 27.013172376464517 28.360330255720346 22.414256593119795 70-71 22.08891540561552 27.071866355697782 27.941687307527737 22.897530931158965 72-73 22.082800650210856 27.22697700158696 27.900712686631916 22.78950966157026 74-75 22.09256636161233 27.533042196236945 27.70344803646381 22.670943405686913 76-77 22.140318454769456 27.108432718359982 28.03741381883193 22.713835008038632 78-79 22.199445021709728 27.317299555679845 27.945640093444652 22.53761532916577 80-81 22.262477425749637 27.378046017241765 27.681936646505317 22.67753991050328 82-83 22.320690182281457 27.43007786298487 27.729676999051488 22.519554955682185 84-85 22.31404460591041 27.513937086934874 27.558101832041377 22.613916475113335 86-87 22.323089126288963 27.473497423879685 27.496425738540065 22.70698771129129 88-89 22.29953279364329 27.63033803754044 27.228993794689565 22.8411353741267 90-91 22.229374515110706 27.573029330347694 27.315980745706092 22.881615408835508 92-93 22.283400329508353 27.222746973359197 27.483788564989663 23.010064132142784 94-95 22.102084236296694 27.180647472337384 27.538691025745482 23.178577265620444 96-97 22.301083563344463 26.886593464871577 27.607795870647365 23.204527101136595 98-99 22.040769887354546 27.115419706819544 27.237319100408026 23.606491305417883 100-101 22.581759427345936 26.56638728343903 27.23375411560155 23.618099173613484 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 807.0 1 517.5 2 346.0 3 472.5 4 395.5 5 365.5 6 710.0 7 1655.5 8 2666.0 9 3105.0 10 2661.5 11 2040.0 12 2015.5 13 2139.5 14 2266.5 15 2415.5 16 2600.5 17 2799.5 18 3115.5 19 3565.0 20 4013.0 21 4720.5 22 5712.5 23 6977.5 24 8782.5 25 10969.0 26 13703.5 27 16949.5 28 19870.0 29 22548.5 30 25575.5 31 29871.0 32 35759.5 33 39064.0 34 40446.5 35 42951.5 36 46875.5 37 50924.5 38 53790.0 39 56585.0 40 59641.0 41 63134.0 42 64956.0 43 66945.0 44 70057.5 45 73525.5 46 75131.0 47 76883.5 48 78570.5 49 76576.5 50 74084.5 51 72109.0 52 70462.5 53 70345.5 54 71042.5 55 67073.5 56 60837.0 57 55538.5 58 51086.5 59 45614.0 60 36785.5 61 29594.5 62 24700.5 63 18681.5 64 13257.0 65 9869.5 66 7190.0 67 5214.5 68 3873.5 69 2862.0 70 2085.5 71 1415.5 72 951.5 73 701.0 74 485.5 75 326.5 76 224.0 77 137.5 78 85.5 79 52.5 80 30.5 81 15.5 82 6.5 83 2.0 84 1.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009255179767405764 2 0.014702238693014364 3 0.009640812257714336 4 0.0 5 0.0010604893483485772 6 0.0018317543289657241 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.230609193285753E-4 14-15 0.0 16-17 0.0029645497692471585 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036394066272871623 26-27 0.019908277312180107 28-29 0.0069654868561986084 30-31 0.0034465903821328755 32-33 0.0 34-35 0.008604424940010045 36-37 8.194690419057187E-4 38-39 0.002892243677314301 40-41 0.002096876666052868 42-43 0.0031091619531128734 44-45 0.0 46-47 1.9281624515428674E-4 48-49 5.543467048185743E-4 50-51 0.00429016145468288 52-53 0.008411608694855759 54-55 0.008194690419057186 56-57 0.017184747849375806 58-59 0.024198438766862986 60-61 0.0037358147498643057 62-63 0.020968766660528684 64-65 0.021836439763722973 66-67 0.013738157467242931 68-69 0.032128006848833025 70-71 0.016943727542932947 72-73 0.005302446741742885 74-75 0.016775013328422943 76-77 0.022535398652407262 78-79 0.026946070260311573 80-81 0.011954607199565778 82-83 0.035815617537408764 84-85 0.022246174284675832 86-87 0.03176647638916874 88-89 0.023330765663668696 90-91 0.02829578397639158 92-93 0.024825091563614416 94-95 0.015666319918785795 96-97 0.0163411767768258 98-99 0.012653566088250068 100-101 0.011689484862478634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2074514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.84572874127487 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3774275631948 42.09507372909272 2 14.03516868527026 15.676084464712767 3 4.6732182960762225 7.829378439343064 4 2.079587322234871 4.645442779652911 5 1.048317915850099 2.9272038981591626 6 0.666792582528423 2.2342510614345867 7 0.4318655776714653 1.6882493532334237 8 0.3062900610121569 1.3683993330746758 9 0.2157011272646702 1.0841387978168981 >10 1.0581967356908366 10.641674693331794 >50 0.06887621741844298 2.619373900551203 >100 0.03338453703383252 3.4714805710628447 >500 0.0034489191692716505 1.3910588262670402 >1k 0.00155201362617223 1.4911183728412607 >5k 1.724459584635811E-4 0.8370717794255975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9309 0.44873160653531374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8075 0.3892477949052163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 0.23750140996879268 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2158 0.1040243642607377 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2140 0.1031566911575434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.8204061288571685E-5 14-15 0.0 0.0 0.0 0.0 4.8204061288571685E-5 16-17 0.0 0.0 0.0 0.0 4.8204061288571685E-5 18-19 0.0 0.0 0.0 0.0 4.8204061288571685E-5 20-21 4.8204061288571685E-5 0.0 0.0 0.0 4.8204061288571685E-5 22-23 4.8204061288571685E-5 0.0 0.0 9.640812257714337E-5 4.8204061288571685E-5 24-25 9.640812257714337E-5 0.0 0.0 2.6512233708714424E-4 4.8204061288571685E-5 26-27 9.640812257714337E-5 0.0 0.0 8.917751338385761E-4 4.8204061288571685E-5 28-29 1.687142145100009E-4 0.0 0.0 0.003542998504710019 4.8204061288571685E-5 30-31 1.9281624515428674E-4 0.0 0.0 0.012364341720518639 4.8204061288571685E-5 32-33 1.9281624515428674E-4 0.0 0.0 0.032899271829450176 4.8204061288571685E-5 34-35 1.9281624515428674E-4 0.0 0.0 0.06242425936870033 4.8204061288571685E-5 36-37 1.9281624515428674E-4 0.0 0.0 0.11012217801374201 4.8204061288571685E-5 38-39 2.410203064428584E-4 0.0 0.0 0.18001806688217098 4.8204061288571685E-5 40-41 2.410203064428584E-4 0.0 0.0 0.25661432026971137 4.8204061288571685E-5 42-43 2.892243677314301E-4 0.0 0.0 0.3325839208605003 7.230609193285752E-5 44-45 3.374284290200018E-4 0.0 0.0 0.42595518757646367 9.640812257714337E-5 46-47 3.856324903085735E-4 0.0 0.0 0.5331369178516028 9.640812257714337E-5 48-49 3.856324903085735E-4 0.0 0.0 0.6414996476283119 9.640812257714337E-5 50-51 3.856324903085735E-4 0.0 0.0 0.7640102693932169 9.640812257714337E-5 52-53 3.856324903085735E-4 0.0 0.0 0.875819589552059 9.640812257714337E-5 54-55 3.856324903085735E-4 0.0 0.0 1.001029638749124 1.4461218386571504E-4 56-57 4.338365515971452E-4 0.0 0.0 1.1430870073665447 1.4461218386571504E-4 58-59 5.302446741742886E-4 0.0 0.0 1.288831986672541 1.4461218386571504E-4 60-61 5.784487354628602E-4 0.0 0.0 1.4451095533700906 1.4461218386571504E-4 62-63 6.266527967514319E-4 0.0 0.0 1.596928244398447 1.4461218386571504E-4 64-65 6.266527967514319E-4 0.0 0.0 1.7532299131266407 1.4461218386571504E-4 66-67 7.230609193285753E-4 0.0 0.0 1.9361643257167702 1.4461218386571504E-4 68-69 7.230609193285753E-4 0.0 0.0 2.1323066510999684 1.4461218386571504E-4 70-71 7.471629499728611E-4 0.0 0.0 2.342548664410074 1.4461218386571504E-4 72-73 7.71264980617147E-4 0.0 0.0 2.5466687619365307 1.4461218386571504E-4 74-75 7.71264980617147E-4 0.0 0.0 2.7537775112628786 1.4461218386571504E-4 76-77 7.71264980617147E-4 0.0 0.0 2.979155599817596 1.4461218386571504E-4 78-79 7.71264980617147E-4 0.0 0.0 3.2301059428859 1.4461218386571504E-4 80-81 8.676731031942904E-4 0.0 0.0 3.4937098520424543 1.4461218386571504E-4 82-83 8.676731031942904E-4 0.0 0.0 3.7610977800101613 1.4461218386571504E-4 84-85 8.676731031942904E-4 0.0 0.0 4.027642136905318 1.4461218386571504E-4 86-87 8.676731031942904E-4 0.0 0.0 4.306261611153262 1.4461218386571504E-4 88-89 8.676731031942904E-4 0.0 0.0 4.602427363710247 1.4461218386571504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4830 0.0 51.43414 1 GTATCAA 8485 0.0 40.6986 1 ATCAACG 10440 0.0 32.798836 3 TATCAAC 10740 0.0 32.151085 2 TCAACGC 10710 0.0 31.925318 4 CAACGCA 10840 0.0 31.54245 5 AACGCAG 11190 0.0 30.598309 6 ACGCAGA 12555 0.0 27.15748 7 CGCAGAG 12645 0.0 26.81397 8 GCAGAGT 13905 0.0 24.008558 9 TGGTATC 2255 0.0 23.169231 2 GAGTACT 8910 0.0 22.413076 12-13 GTGGTAT 2380 0.0 21.953949 1 CAGAGTA 13315 0.0 21.48886 10-11 GTACTTT 9365 0.0 21.298264 14-15 TACATGG 6715 0.0 21.007576 2 AGAGTAC 12480 0.0 20.966913 10-11 GTACATG 7065 0.0 20.70787 1 TGCACCG 425 0.0 20.112951 5 AGTACTT 9270 0.0 19.928886 12-13 >>END_MODULE