FastQCFastQC Report
Thu 26 May 2016
SRR938079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594930
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118310.7417880408544576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95040.5958882207996589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.38453098255095836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25870.1622014759268432No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22790.1428902835861135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.1267767237433618No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.12163543227602465No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16570.10389170684606847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.055.057051
GTATCAA82600.042.6809921
TATCAAC104750.033.342412
ATCAACG106000.032.9043923
TCAACGC107050.032.581654
CAACGCA109150.031.9112555
AACGCAG111650.031.3214516
TACCTGG13600.029.0003242
GTACATG82700.028.784931
TGGTATC20500.028.742962
TACATGG82250.028.3089562
ACGCAGA126800.027.4973357
GTGGTAT22050.027.4767461
CGCAGAG127150.027.4216468
ACATGGG84850.026.3214423
GTACCTG15250.025.3387571
GCAGAGT134400.025.2347589
GAGTACT83600.024.17877812-13
GTAAGGT7700.024.0678654
TAAGGTG8550.023.8982455
Produced by FastQC (version 0.11.5)