FastQCFastQC Report
Thu 26 May 2016
SRR938079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594930
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116700.7316935539490761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99570.624290721222875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64770.4060993272432019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24810.1555554162251635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23790.14916015122920756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.13649501859015756No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.11361000169286427No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16790.10527107772754916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.055.732851
GTATCAA78000.041.5198971
ACCGTAT902.4153862E-531.6631368
TATCAAC102300.031.5179392
ATCAACG102900.031.285983
TCAACGC104100.031.0166954
CAACGCA106200.030.3585955
AACGCAG110250.029.2937836
TACCGTA650.00584792829.227517
TACCTGG13200.028.4614432
TGGTATC19450.027.8733372
CAATCGG700.00839709527.1398329
ACGCAGA123300.026.2318677
AATTCGC1450.026.20602678-79
CGCAGAG123800.026.0875598
GTGGTAT22150.025.7638781
GTACCTG16100.024.8117351
TAAGGTG8250.024.7851185
GTACATG80350.024.7396831
TACATGG79750.024.3891332
Produced by FastQC (version 0.11.5)