##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1594930 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.436453637463714 33.0 31.0 34.0 31.0 34.0 2 32.654291410908314 34.0 31.0 34.0 31.0 34.0 3 32.71345200102826 34.0 31.0 34.0 31.0 34.0 4 36.20202642122225 37.0 35.0 37.0 35.0 37.0 5 36.128140419955734 37.0 35.0 37.0 35.0 37.0 6 36.18817001373101 37.0 37.0 37.0 35.0 37.0 7 36.15635795928348 37.0 36.0 37.0 35.0 37.0 8 36.14798204309907 37.0 36.0 37.0 35.0 37.0 9 37.82016891023431 39.0 38.0 39.0 35.0 39.0 10-11 37.79119052246807 39.0 38.0 39.0 35.0 39.0 12-13 37.707327594314485 39.0 37.5 39.0 35.0 39.0 14-15 38.915391584583645 40.0 38.0 41.0 35.5 41.0 16-17 38.828471468967294 40.0 38.0 41.0 35.0 41.0 18-19 39.08989987021374 40.0 38.5 41.0 36.0 41.0 20-21 39.0662866081897 40.0 39.0 41.0 35.0 41.0 22-23 38.94833848507458 40.0 38.0 41.0 35.0 41.0 24-25 38.836940492686196 40.0 38.0 41.0 35.0 41.0 26-27 38.66850175242801 40.0 38.0 41.0 34.5 41.0 28-29 38.53303091671735 40.0 38.0 41.0 34.0 41.0 30-31 38.260755644448345 40.0 38.0 41.0 34.0 41.0 32-33 38.002532399541046 40.0 37.0 41.0 33.5 41.0 34-35 37.88882960380707 40.0 37.0 41.0 33.0 41.0 36-37 37.82236743932336 40.0 37.0 41.0 33.0 41.0 38-39 37.62506567686356 40.0 37.0 41.0 33.0 41.0 40-41 37.38646743117253 40.0 36.5 41.0 32.5 41.0 42-43 37.107499702181286 40.0 36.0 41.0 31.5 41.0 44-45 36.79440759155574 39.0 36.0 41.0 31.0 41.0 46-47 36.35993993466798 39.0 35.0 41.0 30.0 41.0 48-49 35.89325675734985 39.0 35.0 40.5 29.0 41.0 50-51 35.481529283416826 39.0 35.0 40.0 27.5 41.0 52-53 34.930983804931884 38.0 34.0 40.0 26.0 41.0 54-55 34.54199087107271 38.0 34.0 40.0 25.5 41.0 56-57 34.1515336096255 37.5 33.5 40.0 24.0 41.0 58-59 33.63101452728333 37.0 33.0 40.0 22.5 41.0 60-61 33.14310784799333 36.5 32.5 39.0 21.5 41.0 62-63 32.57317374430225 36.0 31.5 39.0 19.0 40.0 64-65 31.987453367859406 35.0 31.0 38.5 16.0 40.0 66-67 31.42443586865881 35.0 30.5 38.0 10.5 40.0 68-69 30.807464277429105 35.0 29.5 37.5 7.5 40.0 70-71 30.186316327362327 34.0 29.0 37.0 4.5 39.0 72-73 29.509060899224416 34.0 28.5 36.0 2.0 39.0 74-75 28.8543092800311 34.0 27.5 36.0 2.0 38.5 76-77 27.588799194948997 31.5 26.0 34.5 2.0 36.5 78-79 28.255372022596603 33.0 27.0 35.0 2.0 37.0 80-81 27.97975146244663 33.0 26.5 35.0 2.0 36.5 82-83 27.42692908152709 33.0 26.0 35.0 2.0 36.0 84-85 26.789584182377908 33.0 25.0 35.0 2.0 36.0 86-87 26.079301912936618 32.0 23.5 35.0 2.0 35.0 88-89 25.281935884333482 32.0 20.0 34.0 2.0 35.0 90-91 24.63413409992915 31.0 17.5 34.0 2.0 35.0 92-93 23.705381113904686 31.0 4.5 34.0 2.0 35.0 94-95 22.80202955615607 30.5 2.0 34.0 2.0 35.0 96-97 21.756988707968375 30.0 2.0 34.0 2.0 35.0 98-99 20.343282150313804 29.0 2.0 34.0 2.0 35.0 100-101 16.55308289392011 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1912191653419768 1101 2 0.24885801168176158 1101 3 0.141621480784778 1101 4 0.02652942069889974 1101 5 -0.004021302207689814 1101 6 0.053786982958122564 1101 7 0.11301742581775187 1101 8 0.19529134205723864 1101 9 0.23160128527484147 1101 10-11 0.23000216823965758 1101 12-13 0.28238683881114923 1101 14-15 0.39298487834683016 1101 16-17 0.3776018273783919 1101 18-19 0.33069060104200076 1101 20-21 0.20013148401071135 1101 22-23 0.22407458595460383 1101 24-25 0.39879632690418276 1101 26-27 0.3788899945965767 1101 28-29 0.2956046284568359 1101 30-31 0.4789959643330235 1101 32-33 0.5763677072838505 1101 34-35 0.6094078295903174 1101 36-37 0.5584140342193109 1101 38-39 0.6025136824622521 1101 40-41 0.8879343937037021 1101 42-43 0.9340543643047354 1101 44-45 0.8340409009227869 1101 46-47 0.7880267154966205 1101 48-49 0.8422922409058486 1101 50-51 0.78762203214594 1101 52-53 1.0626066611449403 1101 54-55 1.1823257751392688 1101 56-57 1.3103729866245075 1101 58-59 1.4218168103924143 1101 60-61 1.2952567121219758 1101 62-63 1.2668603395876943 1101 64-65 1.355019594612287 1101 66-67 1.568796654577067 1101 68-69 1.713132220194879 1101 70-71 1.8919870238630985 1101 72-73 1.9133582664612767 1101 74-75 1.9594389480330463 1101 76-77 1.8478540074931615 1101 78-79 1.9163109262816356 1101 80-81 2.231042488534481 1101 82-83 2.250942591418898 1101 84-85 2.386246342036408 1101 86-87 2.5275419363642797 1101 88-89 2.8562365701096972 1101 90-91 3.1623256150917953 1101 92-93 3.3696673188314286 1101 94-95 3.467205866618631 1101 96-97 3.489306218154166 1101 98-99 3.5235748049043387 1101 100-101 3.389477501467727 1102 1 0.08578110898697844 1102 2 0.14225760564021783 1102 3 0.13807642579068613 1102 4 0.03886237476265819 1102 5 -0.08033591747217628 1102 6 -0.005541933000202448 1102 7 0.020809184513062462 1102 8 0.09028275271694497 1102 9 0.012101251809873759 1102 10-11 0.05905031316525111 1102 12-13 0.2111020073630101 1102 14-15 0.2723414586738997 1102 16-17 0.289947609677661 1102 18-19 0.3665906969748889 1102 20-21 0.30416903169827947 1102 22-23 0.2588658761404403 1102 24-25 0.25176466904995465 1102 26-27 0.2134899767485976 1102 28-29 0.18835946414387905 1102 30-31 0.21369355262474699 1102 32-33 0.13255426329304498 1102 34-35 0.22131131663937254 1102 36-37 0.2510958047389096 1102 38-39 0.2823616399840674 1102 40-41 0.4094975420873439 1102 42-43 0.317891345318948 1102 44-45 0.1953598666323515 1102 46-47 0.21648810808525099 1102 48-49 0.33926979707121063 1102 50-51 0.38866000372303944 1102 52-53 0.44949787770786287 1102 54-55 0.6397700559772375 1102 56-57 0.5693265372569911 1102 58-59 0.8190788187363438 1102 60-61 0.5497817327587029 1102 62-63 0.8405585525587114 1102 64-65 0.7223801014949203 1102 66-67 0.8183906130358913 1102 68-69 1.0499871830487741 1102 70-71 1.2556279001989061 1102 72-73 1.350371630136177 1102 74-75 1.5962403669474448 1102 76-77 1.6415480931188426 1102 78-79 1.5551514768918153 1102 80-81 2.041866172803715 1102 82-83 1.762165624236868 1102 84-85 2.0400139390290235 1102 86-87 2.329162756032755 1102 88-89 2.4402615126615146 1102 90-91 2.628952681553212 1102 92-93 2.83440082618257 1102 94-95 3.177167270362247 1102 96-97 3.2492614866547136 1102 98-99 3.556499865699017 1102 100-101 3.1898629062822224 1103 1 0.23196944740891468 1103 2 0.19472566449066164 1103 3 0.19515879725481966 1103 4 0.13957300550062968 1103 5 0.04907813131453764 1103 6 0.009400461229645884 1103 7 0.19415148938215765 1103 8 0.14103327664754062 1103 9 0.22573780886777683 1103 10-11 0.2257589120099439 1103 12-13 0.2915070030545408 1103 14-15 0.45284166611849486 1103 16-17 0.3944648488559679 1103 18-19 0.41297796945071497 1103 20-21 0.4213254981188541 1103 22-23 0.4330273212314566 1103 24-25 0.4422897698467523 1103 26-27 0.5079557271129573 1103 28-29 0.47520056157900825 1103 30-31 0.5093451946285583 1103 32-33 0.48882281362761404 1103 34-35 0.47177195003688865 1103 36-37 0.4795053429353118 1103 38-39 0.4251284722518065 1103 40-41 0.5521005463107045 1103 42-43 0.6089300044821471 1103 44-45 0.7126517039532345 1103 46-47 0.7379509116724492 1103 48-49 0.9029640662811076 1103 50-51 1.0001691897955212 1103 52-53 1.0885243242350313 1103 54-55 1.2300438296604952 1103 56-57 1.2354814185880372 1103 58-59 1.3473410180746441 1103 60-61 1.3198929549797427 1103 62-63 1.4292318113538371 1103 64-65 1.3525606314892755 1103 66-67 1.4826005682491434 1103 68-69 1.6570264698648742 1103 70-71 1.652364902996748 1103 72-73 1.928099526647916 1103 74-75 1.9474658858249043 1103 76-77 2.0895699226738493 1103 78-79 2.0244762713088917 1103 80-81 1.9707940565496358 1103 82-83 2.3204458552982423 1103 84-85 2.2923966264472426 1103 86-87 2.2975609645624466 1103 88-89 2.5962837890202763 1103 90-91 2.939666131410128 1103 92-93 2.862642547015323 1103 94-95 2.9806193612974496 1103 96-97 3.232037467761298 1103 98-99 3.517791766849008 1103 100-101 3.5708990122810924 1104 1 0.25127046803724085 1104 2 0.22498540713379356 1104 3 0.20937130837152296 1104 4 0.21619180277915717 1104 5 -0.052002116747203786 1104 6 0.1116944321469191 1104 7 0.1334261622548354 1104 8 0.18678770187511873 1104 9 0.24243133417466822 1104 10-11 0.30882155092218255 1104 12-13 0.32478567669900116 1104 14-15 0.45716161675613876 1104 16-17 0.4770421474273263 1104 18-19 0.46865397141687737 1104 20-21 0.39902538666665777 1104 22-23 0.35400240643261327 1104 24-25 0.3550525095966961 1104 26-27 0.4497089341536409 1104 28-29 0.5505999319509769 1104 30-31 0.43375659856415183 1104 32-33 0.6341994818765357 1104 34-35 0.5937907773732789 1104 36-37 0.8114285356563471 1104 38-39 0.7058992234654013 1104 40-41 0.599161695225618 1104 42-43 0.6683319917735204 1104 44-45 1.001777153891254 1104 46-47 0.9979043566960613 1104 48-49 1.0161066462283443 1104 50-51 1.0971896415464428 1104 52-53 1.1306264207961831 1104 54-55 1.1158881606521902 1104 56-57 1.1888622096258317 1104 58-59 1.356847439100079 1104 60-61 1.4481061337184187 1104 62-63 1.5133129953759479 1104 64-65 1.566587041741485 1104 66-67 1.8251065116222982 1104 68-69 1.9812878820589432 1104 70-71 1.9671779266844318 1104 72-73 2.0889646774258246 1104 74-75 2.1007320006370342 1104 76-77 2.0447772500481847 1104 78-79 2.0596955623102318 1104 80-81 2.1520132453740572 1104 82-83 2.2559347743111324 1104 84-85 2.3439044551332593 1104 86-87 2.487708233239619 1104 88-89 2.600060118795767 1104 90-91 2.9179785854367175 1104 92-93 3.3242766038703273 1104 94-95 3.7335523595301865 1104 96-97 3.9168169764292244 1104 98-99 4.127206873457723 1104 100-101 3.751560940121376 1105 1 0.19271145394434086 1105 2 0.18391841242249996 1105 3 0.17154412379700545 1105 4 0.10613153658460561 1105 5 -0.0704472387688142 1105 6 -0.01742280732796786 1105 7 0.12015799454385956 1105 8 0.09965592666141987 1105 9 0.1559617436440064 1105 10-11 0.20918030355834105 1105 12-13 0.17068712754841187 1105 14-15 0.35950484545598016 1105 16-17 0.32777629967161914 1105 18-19 0.2454005296958286 1105 20-21 0.2861776184922462 1105 22-23 0.28241419295054726 1105 24-25 0.262336725819857 1105 26-27 0.3211633715529416 1105 28-29 0.45162987387359266 1105 30-31 0.40786651660378936 1105 32-33 0.5436893641655871 1105 34-35 0.5512338671251626 1105 36-37 0.5812122266109654 1105 38-39 0.6127553746947214 1105 40-41 0.5766174006577955 1105 42-43 0.5276916305550046 1105 44-45 0.6770379064267473 1105 46-47 0.6559321453640194 1105 48-49 0.8287014543125437 1105 50-51 0.9037663730047001 1105 52-53 1.124612269253781 1105 54-55 1.1771273141455865 1105 56-57 0.993060802229401 1105 58-59 1.0881918633341883 1105 60-61 1.1544923370035676 1105 62-63 1.4275097454991155 1105 64-65 1.4991670360985978 1105 66-67 1.6263772227874007 1105 68-69 1.5388372261313066 1105 70-71 1.8732786147746658 1105 72-73 2.19854285100595 1105 74-75 2.1167214056821066 1105 76-77 2.0792616487646356 1105 78-79 2.228298680488958 1105 80-81 2.2121743862157572 1105 82-83 2.459657923853584 1105 84-85 2.610436791587727 1105 86-87 2.7311088979178884 1105 88-89 2.531514768611359 1105 90-91 2.9647030108622054 1105 92-93 3.3044881797267216 1105 94-95 3.386535492291749 1105 96-97 3.351771415444741 1105 98-99 3.373677415243929 1105 100-101 3.277592555976682 1106 1 0.08118727893248234 1106 2 0.06416051653307164 1106 3 0.049734833553067403 1106 4 0.04739525707080361 1106 5 -0.1175835758008148 1106 6 0.010172734220354585 1106 7 0.003144525699774192 1106 8 0.058887047347170096 1106 9 0.08241716726243453 1106 10-11 0.08788551093385877 1106 12-13 0.08400835039931565 1106 14-15 0.03107858813593367 1106 16-17 -0.12342118254412782 1106 18-19 -0.12187799337059602 1106 20-21 -0.03904556134050097 1106 22-23 -0.008504528298594494 1106 24-25 -0.04896181780009101 1106 26-27 -0.23319088336435811 1106 28-29 0.0791841676640459 1106 30-31 0.022602783804828164 1106 32-33 0.15227474636476046 1106 34-35 -0.069994084782941 1106 36-37 -0.04228149365975753 1106 38-39 -0.1245038832941745 1106 40-41 -0.0721451885932538 1106 42-43 -0.005672000971472357 1106 44-45 -0.035698064388483886 1106 46-47 0.30033403748398513 1106 48-49 0.3194086158995333 1106 50-51 0.487370477200578 1106 52-53 0.4826707311345686 1106 54-55 0.4948779657326341 1106 56-57 0.8374162576411521 1106 58-59 0.7455797633176147 1106 60-61 0.782951618914403 1106 62-63 0.8797524933913294 1106 64-65 0.9014034736995029 1106 66-67 0.9387302666564601 1106 68-69 1.1665603806789164 1106 70-71 1.0590414982128173 1106 72-73 1.4046578530969178 1106 74-75 1.351644756530348 1106 76-77 1.31324922958364 1106 78-79 1.4893472969960655 1106 80-81 1.4570660185056248 1106 82-83 1.8218987676648588 1106 84-85 1.9620605152928334 1106 86-87 1.8167714323331587 1106 88-89 1.824253580157034 1106 90-91 1.9724402635240708 1106 92-93 2.048605647008852 1106 94-95 2.273553134283201 1106 96-97 2.4721260406244276 1106 98-99 2.865340355100919 1106 100-101 2.739315692055147 1107 1 0.2207603000455336 1107 2 0.2105675063673047 1107 3 0.12272995445586332 1107 4 0.1178881424305871 1107 5 0.06294520493162281 1107 6 0.04336055521790172 1107 7 0.1512281181900974 1107 8 0.14210734760669652 1107 9 0.12373113797561786 1107 10-11 0.11974773246296166 1107 12-13 0.13245945873502052 1107 14-15 0.3413927086861932 1107 16-17 0.2151955460013184 1107 18-19 0.22060392024548747 1107 20-21 0.14952047159832205 1107 22-23 0.19013881271006738 1107 24-25 0.27964225540952015 1107 26-27 0.27165588747184444 1107 28-29 0.3170861340245281 1107 30-31 0.35986726547119474 1107 32-33 0.3276555531522547 1107 34-35 0.23932556313923925 1107 36-37 0.2194718710126864 1107 38-39 0.23825234138805484 1107 40-41 0.31826951937952686 1107 42-43 0.41382966623906015 1107 44-45 0.517121690341753 1107 46-47 0.4598294927899431 1107 48-49 0.5092456429571399 1107 50-51 0.33507353593015665 1107 52-53 0.3754699455164783 1107 54-55 0.49942702820246865 1107 56-57 0.7225676651704873 1107 58-59 0.5957671470355166 1107 60-61 0.5504879085376828 1107 62-63 0.4332259833677554 1107 64-65 0.540613260026575 1107 66-67 0.8068015576536105 1107 68-69 1.036313298024325 1107 70-71 0.9853952631206191 1107 72-73 1.0812967671406746 1107 74-75 1.0966137472443762 1107 76-77 1.1573343562658636 1107 78-79 1.0906004679287165 1107 80-81 1.4954356687649124 1107 82-83 1.664079259750796 1107 84-85 1.8213800976885928 1107 86-87 1.8176770074519908 1107 88-89 1.8807002222119777 1107 90-91 1.9712142642134118 1107 92-93 2.2707810542446296 1107 94-95 2.297905372862271 1107 96-97 2.401590563091908 1107 98-99 2.7036687674001385 1107 100-101 2.8301311596101755 1108 1 0.1590895502571641 1108 2 0.15870757653919298 1108 3 0.09921718596864793 1108 4 0.03944340878619812 1108 5 0.03042110376663487 1108 6 0.06448401130339221 1108 7 0.1130727510163041 1108 8 0.1164697292684238 1108 9 0.1203131664600221 1108 10-11 0.12517400731840667 1108 12-13 0.1909152675656287 1108 14-15 0.2505916485704134 1108 16-17 0.25733941798575444 1108 18-19 0.28329221910647107 1108 20-21 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52-53 0.030724264570544335 2316 54-55 0.3648560919627215 2316 56-57 0.43757271239633155 2316 58-59 0.5323498694975726 2316 60-61 0.7979478570245888 2316 62-63 0.9315051354338166 2316 64-65 1.1314213613609923 2316 66-67 1.222438045048765 2316 68-69 1.5161077082423589 2316 70-71 1.4297450961739209 2316 72-73 1.568828219370456 2316 74-75 1.825412177069765 2316 76-77 1.9618638077786272 2316 78-79 1.9957454992611687 2316 80-81 2.0365450341361537 2316 82-83 2.1399234516412022 2316 84-85 2.3567769285924847 2316 86-87 2.3454460014597522 2316 88-89 2.767735047244237 2316 90-91 3.0971969863450397 2316 92-93 3.02557839697522 2316 94-95 3.5791909863686833 2316 96-97 3.631015311515373 2316 98-99 4.115445737842066 2316 100-101 4.448575920160199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 31.0 6 85.0 7 196.0 8 451.0 9 833.0 10 1480.0 11 2535.0 12 3817.0 13 5751.0 14 7545.0 15 10507.0 16 14141.0 17 19760.0 18 20449.0 19 15960.0 20 14993.0 21 16039.0 22 17240.0 23 19078.0 24 21326.0 25 23842.0 26 27325.0 27 31673.0 28 37408.0 29 45080.0 30 53749.0 31 64099.0 32 77476.0 33 97508.0 34 128018.0 35 175527.0 36 229964.0 37 271395.0 38 134210.0 39 5421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.369282664442956 18.58821390280451 14.604151906353257 22.438351526399277 2 16.049084663456895 19.950850121087544 39.8191149008537 24.180950314601866 3 19.489896814394445 24.461829100760994 29.657475454466226 26.390798630378338 4 13.099763684095356 16.617353199168097 35.62194220449069 34.66094091224585 5 13.628214930005008 37.302415269717045 33.59577531802495 15.473594482252997 6 32.498981146508 35.55052572840187 16.793777783350993 15.156715341739135 7 29.054692055450708 33.14822594095039 19.62343174935577 18.17365025424313 8 27.224335172917115 34.57207419027591 18.872474334232432 19.33111630257454 9 27.076987704789552 16.381157793758973 19.707698770478956 36.83415573097252 10-11 25.602095389766323 26.543516016376895 25.973365602252134 21.88102299160465 12-13 26.495896371627598 23.976977046014557 27.092787771250148 22.434338811107697 14-15 23.094437716526027 25.071264905439396 25.89148924764386 25.942808130390716 16-17 22.126707755199288 28.53827440702727 26.621575868533416 22.713441969240026 18-19 22.134545089753153 26.25980450552689 29.825415535478044 21.78023486924191 20-21 22.721404701146756 25.355062604628415 30.262707454245643 21.660825239979182 22-23 22.525311487415106 25.34183361255179 30.244311968941545 21.88854293109156 24-25 22.28946691106741 25.65693672071008 30.221769535365844 21.831826832856663 26-27 21.69508072447461 26.052700633951336 30.13069975423844 22.121518887335615 28-29 21.765312584251344 26.24560325531528 30.125867592935112 21.863216567498263 30-31 21.801865912610584 25.88508586583737 30.071413792454845 22.2416344290972 32-33 21.58561817760027 25.927940411177918 30.053325224304515 22.433116186917292 34-35 22.259570360202776 26.08489269037962 29.779285777215236 21.87625117220237 36-37 22.37626338625135 25.692730669876855 29.71446843728839 22.216537506583403 38-39 21.924732157049256 25.951695625443428 29.84049342106295 22.283078796444375 40-41 22.212293963817455 25.850237760887197 29.83086323489622 22.10660504039913 42-43 21.993338525070193 25.839832409730867 29.647106108316684 22.519722956882248 44-45 21.962710580634482 26.09522621194375 29.68346755484439 22.25859565257738 46-47 22.428194340817463 26.539785445129255 28.8718940643163 22.16012614973698 48-49 22.242606258581883 26.555397415560556 28.71411284507784 22.487883480779722 50-51 22.382800499081466 26.693773394443642 28.61834061682958 22.30508548964531 52-53 22.762127491488656 26.56436332629018 28.42864577128777 22.244863410933398 54-55 22.265836118199545 26.712708394725787 28.452972857742974 22.568482629331697 56-57 22.306997799276456 26.97024320816588 28.3124024251848 22.41035656737286 58-59 22.38816123591631 26.49436025405504 28.282620553880104 22.834857956148547 60-61 22.095233019630957 26.70935401553673 28.65987848996508 22.535534474867237 62-63 22.108305693666807 26.753681979773408 28.75997692688707 22.37803539967271 64-65 21.89581987924235 26.717285398105243 28.67395434282382 22.71294037982858 66-67 21.84616937613857 26.93419991291151 28.37887191879014 22.84075879215978 68-69 22.132865346157836 26.62159844831905 28.570604524189523 22.67493168133359 70-71 22.077990883612447 26.7966305731286 28.199043218197662 22.926335325061288 72-73 22.01720451681265 26.93647997090781 27.995648711855694 23.050666800423844 74-75 21.942812537227336 27.230129221972128 27.896522104418374 22.93053613638216 76-77 22.12586132306747 26.827290225903333 28.259077200880288 22.787771250148907 78-79 22.054259434583336 27.0233803364411 28.11427460766303 22.808085621312536 80-81 22.101791960083503 27.195151886023805 27.971455791619633 22.731600362273067 82-83 22.008038776232535 27.21239555343629 27.959090711015683 22.82047495931549 84-85 21.97353265561917 27.141348736041977 27.893729336103046 22.99138927223581 86-87 22.166025347218522 27.07442336112419 27.786197033147765 22.973354258509527 88-89 21.95394155229383 27.30994463706871 27.48678625394218 23.249327556695278 90-91 21.928966283265215 27.181552589347042 27.75406838275104 23.135412744636703 92-93 21.94895700751757 26.804279811653174 27.860470365470587 23.386292815358665 94-95 21.762592014255176 26.61412306938959 27.998698375069242 23.624586541285993 96-97 22.077534485861126 26.501148326978818 27.809952668739484 23.61136451842057 98-99 21.75227125955371 26.698193651132023 27.523026088919263 24.026509000395002 100-101 22.105045362492397 26.086035123798535 27.78360178816625 24.025317725542813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2683.0 1 1747.0 2 1350.5 3 1768.0 4 1229.0 5 957.0 6 1855.0 7 3849.0 8 5827.5 9 6357.0 10 4835.0 11 3160.5 12 2796.0 13 2776.0 14 2821.5 15 2932.5 16 2987.5 17 2990.0 18 3120.0 19 3281.0 20 3490.5 21 3954.5 22 4570.5 23 5379.5 24 6191.0 25 7466.5 26 9126.0 27 10983.5 28 12539.0 29 14121.5 30 16347.0 31 19134.0 32 22993.5 33 25151.0 34 25984.5 35 27792.0 36 30620.0 37 33608.0 38 36257.0 39 38000.0 40 39780.0 41 43117.5 42 45720.5 43 48126.5 44 50911.0 45 54092.0 46 55264.0 47 56221.5 48 58747.0 49 58933.0 50 58188.0 51 57233.5 52 56665.0 53 57568.0 54 58286.5 55 55260.0 56 50389.5 57 46844.0 58 43979.0 59 39838.5 60 33305.5 61 27219.5 62 22582.0 63 17251.5 64 12348.5 65 9402.0 66 7044.5 67 5398.5 68 4042.5 69 2798.5 70 2003.5 71 1372.0 72 980.0 73 759.5 74 556.5 75 393.5 76 255.0 77 151.0 78 86.0 79 46.5 80 29.5 81 17.5 82 10.5 83 5.5 84 3.5 85 3.5 86 2.0 87 3.0 88 2.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013542914109083157 3 0.0023198510279448 4 0.001943658969359157 5 0.11386079639858802 6 0.0 7 0.0 8 2.507947057237622E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.134933821547027E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.253973528618811E-4 24-25 5.329387496629946E-4 26-27 0.00573692889343106 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011003617713630065 36-37 6.269867643094054E-4 38-39 0.006614710363464228 40-41 0.009749644185011255 42-43 0.005517483525922768 44-45 0.0033857285272707893 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.134933821547027E-5 68-69 9.091308082486378E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.134933821547027E-5 82-83 0.0022258030132983895 84-85 0.003699221909425492 86-87 0.0025706457336685624 88-89 0.0 90-91 0.0024765977190221514 92-93 0.0 94-95 3.134933821547027E-5 96-97 6.269867643094055E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1594930.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.044287037488125 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10021281522562 42.208703190749624 2 12.319687435468445 13.316174479491854 3 3.7559863826199935 6.08968818513635 4 1.7478652380186155 3.7784852250530223 5 0.974978070782341 2.634599735630863 6 0.6147347652339175 1.9933741262534828 7 0.41935518752607714 1.5864626487723281 8 0.3071142751220145 1.32782176304034 9 0.23285374794478753 1.1325973312514708 >10 1.3878880488950192 13.981964476638653 >50 0.09045300589228729 3.3590356359887164 >100 0.04329923842871953 4.124994701707939 >500 0.003598446960580781 1.313713372399262 >1k 0.0016251050789719656 1.3893078814361328 >5k 2.321578684245665E-4 1.0309710883916465 >10k+ 1.1607893421228325E-4 0.7321061580583252 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11670 0.7316935539490761 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9957 0.624290721222875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6477 0.4060993272432019 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2481 0.1555554162251635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2379 0.14916015122920756 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2177 0.13649501859015756 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1812 0.11361000169286427 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1679 0.10527107772754916 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.269867643094055E-5 2 0.0 0.0 0.0 0.0 6.269867643094055E-5 3 0.0 0.0 0.0 0.0 6.269867643094055E-5 4 0.0 0.0 0.0 0.0 6.269867643094055E-5 5 0.0 0.0 0.0 0.0 6.269867643094055E-5 6 0.0 0.0 0.0 0.0 6.269867643094055E-5 7 0.0 0.0 0.0 0.0 6.269867643094055E-5 8 0.0 0.0 0.0 0.0 6.269867643094055E-5 9 0.0 0.0 0.0 0.0 6.269867643094055E-5 10-11 0.0 0.0 0.0 0.0 1.253973528618811E-4 12-13 0.0 0.0 0.0 0.0 2.507947057237622E-4 14-15 0.0 0.0 0.0 0.0 2.507947057237622E-4 16-17 0.0 0.0 0.0 6.269867643094055E-5 3.134933821547027E-4 18-19 0.0 0.0 0.0 9.404801464641083E-5 4.388907350165838E-4 20-21 0.0 0.0 0.0 1.8809602929282162E-4 5.015894114475243E-4 22-23 0.0 0.0 0.0 3.134933821547027E-4 5.642880878784648E-4 24-25 0.0 0.0 0.0 7.837334553867567E-4 5.642880878784648E-4 26-27 0.0 0.0 0.0 0.0015361175725580431 5.642880878784648E-4 28-29 0.0 0.0 0.0 0.005047243452690714 5.642880878784648E-4 30-31 0.0 0.0 0.0 0.016583799915983775 5.642880878784648E-4 32-33 0.0 0.0 0.0 0.038277541961089206 5.642880878784648E-4 34-35 0.0 0.0 0.0 0.06934473613262024 5.642880878784648E-4 36-37 0.0 0.0 0.0 0.12320289918679816 5.642880878784648E-4 38-39 0.0 0.0 0.0 0.1986607562714351 5.642880878784648E-4 40-41 0.0 0.0 0.0 0.2770341018101108 5.642880878784648E-4 42-43 0.0 0.0 0.0 0.3539340284526593 5.642880878784648E-4 44-45 0.0 0.0 0.0 0.4461951308207884 5.642880878784648E-4 46-47 0.0 0.0 0.0 0.5571404387653377 5.956374260939351E-4 48-49 0.0 0.0 0.0 0.6713147285460804 6.269867643094054E-4 50-51 0.0 0.0 0.0 0.7980601029512266 6.269867643094054E-4 52-53 0.0 0.0 0.0 0.9123597900848313 6.269867643094054E-4 54-55 0.0 0.0 0.0 1.0385408764020991 6.269867643094054E-4 56-57 0.0 0.0 0.0 1.1871994382198592 6.583361025248758E-4 58-59 0.0 0.0 0.0 1.3439461292972106 6.89685440740346E-4 60-61 0.0 0.0 0.0 1.506429749267993 6.89685440740346E-4 62-63 0.0 0.0 0.0 1.662956994977836 6.89685440740346E-4 64-65 0.0 0.0 0.0 1.82321481193532 6.89685440740346E-4 66-67 0.0 0.0 0.0 2.0108092518166942 6.89685440740346E-4 68-69 0.0 0.0 0.0 2.2180283774209526 6.89685440740346E-4 70-71 0.0 0.0 0.0 2.4313292746390127 6.89685440740346E-4 72-73 0.0 0.0 0.0 2.6451944599449506 7.210347789558163E-4 74-75 0.0 0.0 0.0 2.864514430100381 7.523841171712865E-4 76-77 0.0 0.0 0.0 3.0992896239960377 7.523841171712865E-4 78-79 0.0 0.0 0.0 3.3575454722150813 7.523841171712865E-4 80-81 0.0 0.0 0.0 3.629939871969303 7.523841171712865E-4 82-83 0.0 0.0 0.0 3.9077577072348006 8.150827936022271E-4 84-85 0.0 0.0 0.0 4.185199350441712 8.777814700331676E-4 86-87 0.0 0.0 0.0 4.459537409165293 9.404801464641081E-4 88-89 0.0 0.0 0.0 4.758735493093741 0.0010031788228950487 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4420 0.0 55.73285 1 GTATCAA 7800 0.0 41.519897 1 ACCGTAT 90 2.4153862E-5 31.663136 8 TATCAAC 10230 0.0 31.517939 2 ATCAACG 10290 0.0 31.28598 3 TCAACGC 10410 0.0 31.016695 4 CAACGCA 10620 0.0 30.358595 5 AACGCAG 11025 0.0 29.293783 6 TACCGTA 65 0.005847928 29.22751 7 TACCTGG 1320 0.0 28.461443 2 TGGTATC 1945 0.0 27.873337 2 CAATCGG 70 0.008397095 27.139832 9 ACGCAGA 12330 0.0 26.231867 7 AATTCGC 145 0.0 26.206026 78-79 CGCAGAG 12380 0.0 26.087559 8 GTGGTAT 2215 0.0 25.763878 1 GTACCTG 1610 0.0 24.811735 1 TAAGGTG 825 0.0 24.785118 5 GTACATG 8035 0.0 24.739683 1 TACATGG 7975 0.0 24.389133 2 >>END_MODULE