FastQCFastQC Report
Thu 26 May 2016
SRR938078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118200.7501689784183619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95510.6061644596339911No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60810.3859371876279238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24240.153841760041126No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.14667009383458837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.12801106848306568No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.11804689508106204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.054.71411
GTATCAA84800.044.220521
TATCAAC107200.034.7583852
ATCAACG108800.034.3356863
TCAACGC110700.033.7463654
CAACGCA111450.033.3913355
AACGCAG115200.032.4229976
ACAATCG1004.9502516E-528.5033848
CGCAGAG132250.028.1980518
ACGCAGA132350.028.1408547
GCAGAGT140700.025.8622329
TGGTATC20350.025.223052
GTGGTAT21450.025.1332281
GTACATG83300.025.0857031
TACATGG84150.024.6246812
GTACCTG13800.024.200041
GAGTACT85550.023.65601512-13
TACCTGG13700.023.5899282
ACATGGG86750.022.8464453
CAGAGTA136200.022.2871810-11
Produced by FastQC (version 0.11.5)