FastQCFastQC Report
Thu 26 May 2016
SRR938078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117450.745409022971545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99830.6335818030076571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66360.42116085793436975No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.1563804029460951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24250.15390522611375024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.13258062571201001No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.11849115758943163No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15840.1005302590367754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.054.4045831
GTATCAA84300.040.011121
TATCAAC108200.031.2170772
ATCAACG107700.031.1806163
TCAACGC109050.030.9243014
CAACGCA111250.030.2273755
AACGCAG115200.029.1497066
GGACCGA4150.026.32376
ACGCAGA128100.026.1400977
CGCAGAG128750.025.8236738
TACCTGG12950.025.6790052
TGGTATC20800.025.123452
GTACATG84600.024.3146171
GCAGAGT137100.023.9044559
GTACCTG14000.023.413751
GAGTACT83550.023.22270412-13
TACATGG85200.023.0839772
GTGGTAT24200.022.7715451
GTACTTT86400.022.45667814-15
ACATGGG84200.022.4518413
Produced by FastQC (version 0.11.5)