FastQCFastQC Report
Thu 26 May 2016
SRR938077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598945
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120840.7557483215495218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98520.6161562780458364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.3971368621184594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25240.15785408503732148No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.14422009512522319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.13214963616634717No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.12170524939882235No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16560.1035682903414439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.054.6553461
GTATCAA84150.040.549541
TATCAAC105250.032.148892
ATCAACG106250.031.7568573
TCAACGC106950.031.5490064
CAACGCA109000.030.9120545
AACGCAG112150.030.1285656
ACGCAGA125700.026.702657
CGCAGAG125950.026.5364048
GTACATG86500.026.427341
TGGTATC21250.026.1658612
TACATGG85700.026.0630462
GTGGTAT22600.025.7623441
TACCTGG13800.025.4835972
ATAACGC1701.11804184E-725.1594813
ACATGGG86700.024.7757853
GCAGAGT135600.024.1563229
GTACCTG15900.023.7117731
CATGGGG40350.023.0845094
GAGTACT80550.022.9569712-13
Produced by FastQC (version 0.11.5)