FastQCFastQC Report
Thu 26 May 2016
SRR938077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122450.7658174608882732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.6570582477821313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64710.40470435193205523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26100.16323263151640613No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.1612938531344105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.13827867750297854No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.11388759463271095No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17150.10725822339104849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.054.0924341
GTATCAA82400.040.1847841
ATCAACG107000.030.9421673
TATCAAC107350.030.800892
TCAACGC108550.030.4984344
CAACGCA111450.029.7048455
AACGCAG113900.029.1075926
TGGTATC20700.025.474682
ACGCAGA131050.025.2621547
CGCAGAG131050.025.2259088
TACCTGG14100.024.9326652
GTACATG81300.024.4838431
GTGGTAT22900.024.4795041
TACATGG81700.023.6661972
CATGGGG39250.023.476684
ATGGGGA22200.023.3210535
GCAGAGT141850.023.3052889
GAGTACT87000.022.62975712-13
ACATGGG82950.022.3904153
GTACCTG15700.022.0891631
Produced by FastQC (version 0.11.5)