##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1598945 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.469912973867146 33.0 31.0 34.0 31.0 34.0 2 32.67623339139245 34.0 31.0 34.0 31.0 34.0 3 32.73015206902051 34.0 31.0 34.0 31.0 34.0 4 36.213205582430916 37.0 35.0 37.0 35.0 37.0 5 36.178648421302796 37.0 35.0 37.0 35.0 37.0 6 36.2220770570595 37.0 37.0 37.0 35.0 37.0 7 36.18494444774523 37.0 37.0 37.0 35.0 37.0 8 36.17667837230174 37.0 37.0 37.0 35.0 37.0 9 37.86756955367446 39.0 38.0 39.0 35.0 39.0 10-11 37.85397027414952 39.0 38.0 39.0 35.0 39.0 12-13 37.801062888342 39.0 38.0 39.0 35.0 39.0 14-15 39.090937461888934 40.0 38.5 41.0 36.0 41.0 16-17 39.045778622779395 40.0 38.0 41.0 36.0 41.0 18-19 39.08768813186195 40.0 38.5 41.0 36.0 41.0 20-21 39.02246324920495 40.0 38.5 41.0 35.0 41.0 22-23 38.8721372530012 40.0 38.0 41.0 35.0 41.0 24-25 38.82587049585821 40.0 38.0 41.0 35.0 41.0 26-27 38.78670123112427 40.0 38.0 41.0 35.0 41.0 28-29 38.72284006016467 40.0 38.0 41.0 35.0 41.0 30-31 38.49592919081019 40.0 38.0 41.0 34.0 41.0 32-33 38.2873253926808 40.0 38.0 41.0 34.0 41.0 34-35 38.07916251028021 40.0 37.0 41.0 34.0 41.0 36-37 37.94238638602329 40.0 37.0 41.0 33.0 41.0 38-39 37.91851752249139 40.0 37.0 41.0 33.0 41.0 40-41 37.84233009890897 40.0 37.0 41.0 33.0 41.0 42-43 37.66894702444424 40.0 37.0 41.0 33.0 41.0 44-45 37.426114093980715 40.0 37.0 41.0 33.0 41.0 46-47 37.05257654265782 40.0 36.5 41.0 31.5 41.0 48-49 36.66540687765996 40.0 36.0 41.0 31.0 41.0 50-51 36.344886159311294 39.5 35.5 41.0 30.0 41.0 52-53 35.913772518754556 39.0 35.0 41.0 29.0 41.0 54-55 35.68561176275607 39.0 35.0 40.5 28.0 41.0 56-57 35.438144213841 38.5 35.0 40.0 28.0 41.0 58-59 35.10426249808468 38.0 35.0 40.0 27.5 41.0 60-61 34.742198762308895 38.0 34.0 40.0 26.5 41.0 62-63 34.37622744997483 37.0 34.0 40.0 26.0 41.0 64-65 33.97945614139323 36.5 34.0 39.5 26.0 41.0 66-67 33.55666924128097 36.0 33.0 39.0 25.0 41.0 68-69 33.11006726310161 35.5 33.0 39.0 23.5 40.0 70-71 32.6063038440972 35.0 32.5 38.0 22.5 40.0 72-73 32.19212918518148 35.0 32.0 37.0 21.5 39.5 74-75 31.759048622685583 35.0 31.5 37.0 20.0 39.0 76-77 30.477835385207122 33.5 30.0 35.5 19.5 37.5 78-79 31.198807338588882 34.5 31.0 36.0 20.0 38.0 80-81 31.052996507071853 35.0 31.0 35.5 19.0 37.0 82-83 30.70140842868266 34.5 31.0 35.0 18.0 37.0 84-85 30.33129282120398 34.0 31.0 35.0 13.5 36.0 86-87 29.909013443239136 34.0 30.5 35.0 7.0 36.0 88-89 29.460379187526776 34.0 30.0 35.0 4.5 35.5 90-91 29.13267560797901 34.0 29.5 35.0 2.0 35.0 92-93 28.729921917264196 34.0 29.0 35.0 2.0 35.0 94-95 28.331358176797828 34.0 29.0 35.0 2.0 35.0 96-97 27.763326756079792 34.0 28.0 35.0 2.0 35.0 98-99 27.077260631228718 33.0 27.0 35.0 2.0 35.0 100-101 24.67154060958945 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05812111064193459 1101 2 0.023744070946243312 1101 3 0.11030767181588885 1101 4 0.10411240931632904 1101 5 0.05783508245936275 1101 6 0.03418177336923378 1101 7 0.017251663199516543 1101 8 -0.12104020391583958 1101 9 -0.07664833777887026 1101 10-11 -0.0029300629958370905 1101 12-13 0.15291296026040158 1101 14-15 0.029170355950093096 1101 16-17 0.004994107741133291 1101 18-19 0.04964452668425423 1101 20-21 0.04850663955708967 1101 22-23 0.160338504239796 1101 24-25 0.24786981240605854 1101 26-27 0.16137587611694215 1101 28-29 0.18064365356791257 1101 30-31 0.17394185858965017 1101 32-33 0.21953198954219033 1101 34-35 0.2087288031827228 1101 36-37 0.3017325547842731 1101 38-39 0.2882063093155409 1101 40-41 0.3092744575036406 1101 42-43 0.4044920132938188 1101 44-45 0.26759121600992586 1101 46-47 0.2009052534111717 1101 48-49 0.29006255385654356 1101 50-51 0.390255598662435 1101 52-53 0.3668497220597544 1101 54-55 0.5231007414818905 1101 56-57 0.5190048966406096 1101 58-59 0.490179459939462 1101 60-61 0.4240841155571573 1101 62-63 0.3970469039484641 1101 64-65 0.43521009452183534 1101 66-67 0.34602612919547227 1101 68-69 0.21920761829733948 1101 70-71 0.16784562719082174 1101 72-73 0.06933281686175263 1101 74-75 0.13507088207538587 1101 76-77 0.0484679096328442 1101 78-79 0.18148682521932002 1101 80-81 0.31866265690879914 1101 82-83 0.39368387307796127 1101 84-85 0.3086782327962361 1101 86-87 0.5552142015710899 1101 88-89 0.5800712746348076 1101 90-91 0.41657761688930606 1101 92-93 0.510392682321573 1101 94-95 0.5377836009699735 1101 96-97 0.6487146836878175 1101 98-99 0.6785445614629246 1101 100-101 0.6606823353317743 1102 1 0.1582735930896959 1102 2 0.1920549508202356 1102 3 0.2112749299804051 1102 4 0.06584936871578151 1102 5 0.09664898684783907 1102 6 0.08249650719520218 1102 7 0.12467429092450999 1102 8 0.13914553981311428 1102 9 0.09203746459717621 1102 10-11 0.169048354873091 1102 12-13 0.08812991262221459 1102 14-15 0.19739012337247175 1102 16-17 0.17921158792679392 1102 18-19 0.22124069116421907 1102 20-21 0.22772273734693016 1102 22-23 0.20128988579779872 1102 24-25 0.21978099556931596 1102 26-27 0.10061329631501081 1102 28-29 0.15502115654216198 1102 30-31 0.09048494744838109 1102 32-33 0.10785535286932202 1102 34-35 0.40425507708141595 1102 36-37 0.40437858605154986 1102 38-39 0.29173037138092894 1102 40-41 0.4326417642614544 1102 42-43 0.3738080624903617 1102 44-45 0.41031834783183285 1102 46-47 0.2699276482234083 1102 48-49 0.3072786181337648 1102 50-51 0.22608457535440607 1102 52-53 0.23145347714948628 1102 54-55 0.3246714363661809 1102 56-57 0.41771193764330405 1102 58-59 0.321732435022831 1102 60-61 0.1545860509112984 1102 62-63 0.15707346270448141 1102 64-65 0.1999137591274689 1102 66-67 0.11203888074961554 1102 68-69 0.18921800721135895 1102 70-71 0.10702021121534955 1102 72-73 0.030209967953418015 1102 74-75 0.1669005457761905 1102 76-77 0.15298331706259205 1102 78-79 0.2306131929533599 1102 80-81 0.27931464504532855 1102 82-83 0.38311482869048774 1102 84-85 0.35021608530717074 1102 86-87 0.3313456716024241 1102 88-89 0.22740534524591283 1102 90-91 0.11852051137480402 1102 92-93 0.16240700059098145 1102 94-95 -0.07026005760339515 1102 96-97 0.10287883690754285 1102 98-99 0.1685054354722091 1102 100-101 0.03823243670746734 1103 1 -0.011275866649782529 1103 2 0.08185335931868565 1103 3 0.10101360331129428 1103 4 0.07339166806927011 1103 5 0.09739420754480221 1103 6 0.010514651017906829 1103 7 0.009400596137922435 1103 8 -0.03631133629223626 1103 9 -0.022915520888162177 1103 10-11 0.0036678712336808417 1103 12-13 -0.029992219355300165 1103 14-15 0.13160921825738114 1103 16-17 0.09263745854237726 1103 18-19 0.10459724940013615 1103 20-21 0.11477553148387187 1103 22-23 0.15096374157555914 1103 24-25 0.04780051036307498 1103 26-27 0.054261499265727764 1103 28-29 0.04740335585393041 1103 30-31 0.042239496624134176 1103 32-33 0.14088841089861148 1103 34-35 0.11714093198958864 1103 36-37 0.016943878548225655 1103 38-39 -0.09239956142190664 1103 40-41 -0.036395588824298386 1103 42-43 -0.1256638688357512 1103 44-45 -0.057231256838868205 1103 46-47 -0.23411696121630854 1103 48-49 -0.21300857092510483 1103 50-51 -0.2123921191168563 1103 52-53 -0.16301186372288612 1103 54-55 -0.08003114125525457 1103 56-57 -0.0669723570998002 1103 58-59 -0.17194934692618347 1103 60-61 -0.1854226057916719 1103 62-63 -0.1563726297342285 1103 64-65 -0.14518976877276657 1103 66-67 -0.29957461508948313 1103 68-69 -0.11535986100698636 1103 70-71 -0.2142744457187291 1103 72-73 -0.35242707529288 1103 74-75 -0.44827669140376614 1103 76-77 -0.3933753348419806 1103 78-79 -0.3800638317260763 1103 80-81 -0.22719061262868223 1103 82-83 -0.12992628869009337 1103 84-85 -0.11532210896726625 1103 86-87 -0.1816753708447969 1103 88-89 -0.43303915294930206 1103 90-91 -0.4720644428053866 1103 92-93 -0.21546077708451605 1103 94-95 -0.4563636990983788 1103 96-97 -0.4866663826143025 1103 98-99 -0.33675884857732896 1103 100-101 -0.21397115445329362 1104 1 0.1839201711346945 1104 2 0.12252702560063966 1104 3 0.06647151037403631 1104 4 0.024904279702674614 1104 5 0.1868730462212227 1104 6 0.14674001323118802 1104 7 0.07767396211620792 1104 8 0.03634691141346735 1104 9 0.10745481628114106 1104 10-11 0.11450211033366742 1104 12-13 0.09072021747447678 1104 14-15 0.18697042305775824 1104 16-17 0.11164735287582772 1104 18-19 0.2349155936960443 1104 20-21 0.22012609119163784 1104 22-23 0.2024555112381634 1104 24-25 0.09920980857133799 1104 26-27 0.131400322478747 1104 28-29 0.1737646390918428 1104 30-31 0.21632082513169593 1104 32-33 0.2172043124259062 1104 34-35 0.16157153158843585 1104 36-37 0.2238903057189887 1104 38-39 0.2010605418915219 1104 40-41 0.32416109349251343 1104 42-43 0.3330853888091099 1104 44-45 0.24033807633012572 1104 46-47 0.34328877368727007 1104 48-49 0.3141908732890073 1104 50-51 0.44043846703556255 1104 52-53 0.44591141317278016 1104 54-55 0.46330805621681037 1104 56-57 0.44991636246363953 1104 58-59 0.5008850490488541 1104 60-61 0.5016150330147369 1104 62-63 0.384015075744081 1104 64-65 0.37413589262364155 1104 66-67 0.35697570258248845 1104 68-69 0.4537536829124278 1104 70-71 0.32642342414221304 1104 72-73 0.285723143771925 1104 74-75 0.27267441960774264 1104 76-77 0.41210610347811283 1104 78-79 0.5374030844466198 1104 80-81 0.49749210758466944 1104 82-83 0.4017180979844923 1104 84-85 0.5609366452207389 1104 86-87 0.40530287955018807 1104 88-89 0.45140623772559607 1104 90-91 0.606278508462502 1104 92-93 0.4752002271399043 1104 94-95 0.5292439115825722 1104 96-97 0.5917841401157737 1104 98-99 0.7410083712606443 1104 100-101 0.8994741577840806 1105 1 0.06638424277475963 1105 2 0.022386510378154867 1105 3 0.050634351216302775 1105 4 0.07370783694560856 1105 5 -0.011262496289276669 1105 6 -0.04612775596069696 1105 7 -0.036654670181661686 1105 8 0.03625694865722551 1105 9 -0.033668273815735006 1105 10-11 -0.013143279590920542 1105 12-13 -0.00980408381821718 1105 14-15 -0.04815838256285332 1105 16-17 -0.011112605444175472 1105 18-19 0.024699868783990553 1105 20-21 0.12924101035928004 1105 22-23 0.04519772461083704 1105 24-25 -0.002857271456313981 1105 26-27 -0.14238048615437293 1105 28-29 -0.12199390582247815 1105 30-31 -0.03654075396227796 1105 32-33 -0.09708738562112273 1105 34-35 0.02594277239356302 1105 36-37 -2.331248332794189E-4 1105 38-39 -0.11641183479657258 1105 40-41 -0.09363419996970634 1105 42-43 -0.09322644768976573 1105 44-45 -0.21658338164427704 1105 46-47 -0.24801785281254496 1105 48-49 -0.19158737677031468 1105 50-51 -0.21059146208781954 1105 52-53 -0.2682960995427095 1105 54-55 -0.25060274447235287 1105 56-57 -0.17033247947221497 1105 58-59 -0.29357048025610055 1105 60-61 -0.24186627804800054 1105 62-63 -0.17511542183662954 1105 64-65 -0.33068454295290906 1105 66-67 -0.35436517579240245 1105 68-69 -0.2278452193768814 1105 70-71 -0.18419775218273315 1105 72-73 -0.1783414808502215 1105 74-75 -0.16400624355807736 1105 76-77 -0.1721745555609786 1105 78-79 0.04354675661903684 1105 80-81 -0.09749061172534113 1105 82-83 0.16889696086350625 1105 84-85 0.1811629964675312 1105 86-87 0.02868081597788219 1105 88-89 0.055919025436185876 1105 90-91 -0.08907252167296065 1105 92-93 0.09128024447217697 1105 94-95 -0.09809919522756871 1105 96-97 0.17073904525252104 1105 98-99 0.07017157319195277 1105 100-101 -0.04438268588499383 1106 1 -0.12323594103972368 1106 2 -0.06626468614106074 1106 3 -0.056351573590347925 1106 4 -0.042424088442452046 1106 5 -0.0713727722026789 1106 6 -0.04139263218780087 1106 7 -0.06133577409083557 1106 8 -0.06661639057538338 1106 9 -0.11932058523338185 1106 10-11 -0.09137664476327956 1106 12-13 -0.1724796244161766 1106 14-15 -0.24490421287467257 1106 16-17 -0.13461835403937528 1106 18-19 -0.13028578768034293 1106 20-21 -0.018692873608230798 1106 22-23 -0.005973797241487944 1106 24-25 -0.09993868938988015 1106 26-27 -0.10066882955586465 1106 28-29 -0.08993179096557924 1106 30-31 -0.01730216729570344 1106 32-33 -0.042327184619949776 1106 34-35 0.07898290713973921 1106 36-37 -0.001405525187230694 1106 38-39 -0.011211863866989802 1106 40-41 0.07291473416822924 1106 42-43 -0.09237250417582032 1106 44-45 0.039513619310895365 1106 46-47 0.022104825946563267 1106 48-49 0.05191430675826325 1106 50-51 -0.08794444883362473 1106 52-53 -0.08875073212138318 1106 54-55 -0.4166998492504419 1106 56-57 -0.3137143273924039 1106 58-59 -0.23057891866356073 1106 60-61 -0.2664437586970081 1106 62-63 -0.2507641952886317 1106 64-65 -0.4578402661514218 1106 66-67 -0.4430059900196568 1106 68-69 -0.48370262897906 1106 70-71 -0.37021987285371694 1106 72-73 -0.2925241309222635 1106 74-75 -0.2901395106577134 1106 76-77 -0.1601993804197015 1106 78-79 -0.26655693053215757 1106 80-81 -0.5812059867816437 1106 82-83 -0.5505898823766664 1106 84-85 -0.16468871884682912 1106 86-87 -0.015313746895674996 1106 88-89 -0.11664964144557999 1106 90-91 -0.28742541098495167 1106 92-93 -0.11229373974034829 1106 94-95 -0.2710094794468034 1106 96-97 -0.2884513595929725 1106 98-99 -0.10016829512864334 1106 100-101 -0.1538919069575364 1107 1 0.13328207880698528 1107 2 0.14995287065647744 1107 3 0.15577920040686166 1107 4 0.09136682517156203 1107 5 0.09102256861310565 1107 6 0.07412623012145758 1107 7 0.11910688503843403 1107 8 0.10219433311826975 1107 9 0.11685280332805803 1107 10-11 0.15926931950531298 1107 12-13 0.14024320082473452 1107 14-15 0.2547365577343683 1107 16-17 0.09900971400473679 1107 18-19 0.18650417053229518 1107 20-21 0.23061671154000862 1107 22-23 0.11415686076450271 1107 24-25 0.11493371263568974 1107 26-27 0.07395328325475248 1107 28-29 0.0621861171632645 1107 30-31 0.09937298250086002 1107 32-33 0.19217883013535442 1107 34-35 0.3037191415763161 1107 36-37 0.34491373759698973 1107 38-39 0.28560299430321834 1107 40-41 0.23329568138917267 1107 42-43 0.3495511873363668 1107 44-45 0.32418831735217424 1107 46-47 0.4194604168760492 1107 48-49 0.4878316370676856 1107 50-51 0.6182125864918078 1107 52-53 0.7887232385328389 1107 54-55 0.7111396463708886 1107 56-57 0.7258821757775138 1107 58-59 0.7305829089307565 1107 60-61 0.691679404139407 1107 62-63 0.6830862782913201 1107 64-65 0.679256206494486 1107 66-67 0.7626220261336485 1107 68-69 0.7622382506006815 1107 70-71 0.7471424486190088 1107 72-73 0.6838832928211431 1107 74-75 0.7366212666055709 1107 76-77 0.5858580627064462 1107 78-79 0.7067149147355529 1107 80-81 0.8357080515137731 1107 82-83 0.8072212869674686 1107 84-85 0.923200576689645 1107 86-87 0.8921260216102631 1107 88-89 0.9882539255897171 1107 90-91 1.0563878309391619 1107 92-93 1.0241916804683733 1107 94-95 0.9819456258457571 1107 96-97 1.1506234883211697 1107 98-99 1.2135971156786916 1107 100-101 1.1232431709244644 1108 1 0.06287059697037023 1108 2 -0.01021752222587935 1108 3 0.11565380742518272 1108 4 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 19.0 5 33.0 6 62.0 7 154.0 8 357.0 9 681.0 10 1173.0 11 1956.0 12 2936.0 13 4558.0 14 6052.0 15 8223.0 16 10862.0 17 15891.0 18 15836.0 19 10509.0 20 8405.0 21 8539.0 22 8695.0 23 9205.0 24 10451.0 25 12201.0 26 14951.0 27 18006.0 28 21810.0 29 26871.0 30 32714.0 31 40064.0 32 50396.0 33 66453.0 34 93612.0 35 147111.0 36 234454.0 37 371733.0 38 310259.0 39 33708.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32016110622942 18.611146724871713 14.595623989568121 22.473068179330745 2 16.023301388688203 20.016413305035393 39.88483164164951 24.075453664626888 3 19.50011477020628 24.47951555089386 29.63304181075487 26.387327868144983 4 13.093633614664668 16.653668512675544 35.57214288171263 34.68055499094716 5 13.57458422558889 37.39630656233114 33.5237506754458 15.505358536634178 6 32.54058144589088 35.51823233444553 16.793448179893616 15.147738039769973 7 28.966162063110364 33.186820059476716 19.690045623833218 18.156972253579703 8 27.20543260589216 34.65650325403487 18.82655862828953 19.311505511783448 9 27.13282724615275 16.38185190883426 19.7402144178559 36.74510642715709 10-11 25.585120188624373 26.575085446966597 26.00924359499545 21.83055076941358 12-13 26.50772853350178 24.01946283330571 27.083076653668513 22.389731979524 14-15 23.111926926817368 25.01646398093743 25.943637836198242 25.927971256046956 16-17 22.09417918041404 28.616775535565246 26.641625100105287 22.647420183915422 18-19 22.11064170437382 26.287270669097435 29.812751533042096 21.789336093486643 20-21 22.700687015500844 25.291113828180457 30.34247581999381 21.665723336324888 22-23 22.508466520111696 25.354624455500346 30.278527404007015 21.85838162038094 24-25 22.202527560365112 25.651966971343228 30.373067209388726 21.77243825890294 26-27 21.652193087399777 26.05838537097246 30.192558813088304 22.09686272853946 28-29 21.74837158251222 26.194678366047615 30.214516446782095 21.84243360465807 30-31 21.794151768822566 25.80689141902942 30.15441431694023 22.244542495207778 32-33 21.547541131245676 25.951812345268245 30.0800250666615 22.420621456824584 34-35 22.25433309062521 26.038609357524077 29.85453776613446 21.85251978571625 36-37 22.33204232344254 25.68617670834695 29.782644386452372 22.19913658175814 38-39 21.849120596806912 25.932745375603407 29.898801544561916 22.31933248302777 40-41 22.158520840787343 25.795838097644825 29.936473066732056 22.10916799483578 42-43 21.983046013317505 25.8048546470536 29.747160053990562 22.464939285638337 44-45 21.933723374879875 26.08663700649732 29.763126219791925 22.216513398830877 46-47 22.400395260624975 26.48052309491571 28.97235364568512 22.14672799877419 48-49 22.22612410057882 26.556354346146993 28.77663084096076 22.440890712313433 50-51 22.379600298947118 26.73616040576755 28.657083264277382 22.227156031007947 52-53 22.700030332500493 26.57108280772634 28.505170596862307 22.223716262910855 54-55 22.215773525668485 26.771715099643828 28.577280644424917 22.435230730262766 56-57 22.217180703526388 26.99845835848012 28.41310989433658 22.371251043656912 58-59 22.30392540081116 26.537247997898618 28.332588050245633 22.826238551044593 60-61 22.050007974007862 26.723058016379554 28.768531750623065 22.458402258989523 62-63 22.02433479575595 26.76483556344965 28.89511521659594 22.315714424198454 64-65 21.814884189262294 26.755735813301897 28.736792072272653 22.692587925163153 66-67 21.718132893876902 26.964373383699876 28.504951702528853 22.81254201989437 68-69 22.023775088847785 26.629537960245596 28.712696047543922 22.63399090336269 70-71 21.998348911313396 26.771527475929442 28.376585811269305 22.853537801487857 72-73 21.934119059755027 26.919281151008946 28.1880239783107 22.958575810925332 74-75 21.846561326374577 27.291776765304622 28.08739512616131 22.774266782159486 76-77 21.996535215407658 26.888979921135498 28.329429717720124 22.78505514573672 78-79 21.95188077138363 27.14502375003518 28.149404763766107 22.753690714815082 80-81 21.991938434405185 27.24105582118209 28.02266494469791 22.744340799714813 82-83 21.932149010753964 27.26094393490708 28.012345640406643 22.794561413932314 84-85 21.85543785739169 27.24892129705781 27.965815483163226 22.929825362387273 86-87 22.02925062854141 27.18382178193053 27.862396337573642 22.92453125195442 88-89 21.920929713320255 27.30823365038624 27.66449992479414 23.106336711499367 90-91 21.80555755340566 27.304353318852915 27.859095488826668 23.030993638914758 92-93 21.866789497811993 26.97844763697017 27.934297845514195 23.220465019703642 94-95 21.636297683785248 26.744447119819633 28.183333385451032 23.43592181094409 96-97 21.950567405382923 26.601884367504823 28.003996385116437 23.443551841995816 98-99 21.648962284506347 26.788601233938632 27.745638530405987 23.816797951149038 100-101 21.579166262754505 26.599632882932184 28.09274240202133 23.72845845229198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2827.0 1 1837.5 2 1397.5 3 1831.0 4 1267.0 5 980.5 6 1857.5 7 3932.5 8 6159.5 9 6741.5 10 5122.5 11 3308.5 12 2836.5 13 2849.5 14 2961.5 15 3054.0 16 2999.0 17 3012.5 18 3136.0 19 3279.0 20 3476.5 21 3938.5 22 4560.0 23 5265.0 24 6138.5 25 7364.5 26 8996.5 27 10986.0 28 12560.5 29 14110.0 30 16441.0 31 19187.5 32 23037.0 33 25272.0 34 26135.5 35 27953.5 36 30744.5 37 33798.0 38 36262.0 39 38144.0 40 40167.0 41 43511.0 42 46001.0 43 48463.5 44 51408.5 45 54684.5 46 55802.5 47 56818.5 48 59496.0 49 59421.0 50 58531.0 51 57599.5 52 56563.5 53 57716.0 54 59361.5 55 55943.5 56 50597.0 57 46628.0 58 43585.5 59 39588.5 60 32936.0 61 27093.5 62 22232.0 63 16510.5 64 11691.0 65 8785.0 66 6498.0 67 5069.5 68 3704.0 69 2418.5 70 1722.5 71 1202.5 72 879.5 73 659.0 74 470.0 75 327.5 76 209.0 77 121.5 78 79.5 79 48.0 80 23.5 81 10.0 82 7.5 83 4.5 84 2.5 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015134979627191679 3 0.006129041336631342 4 0.0 5 6.254123812889124E-5 6 0.0 7 0.0 8 4.377886669022387E-4 9 6.879536194178036E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1889433345111936E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005691252669729103 26-27 0.02142037405914525 28-29 0.0 30-31 0.0 32-33 5.316005240955756E-4 34-35 0.019825572486858522 36-37 0.004221533573700158 38-39 0.019012536391182937 40-41 0.015822933246609484 42-43 0.009193562004947012 44-45 0.0034710387161534634 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.563530953222281E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002126402096382302 86-87 3.127061906444562E-4 88-89 3.127061906444562E-5 90-91 2.5016495251556494E-4 92-93 1.2508247625778247E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1598945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.55007404245059 #Duplication Level Percentage of deduplicated Percentage of total 1 77.97897023278047 41.757796297194446 2 12.183932408522121 13.049009652091462 3 3.8805403098139903 6.234096627457601 4 1.8232735039793682 3.9054572455093397 5 0.9693829969958654 2.595526563231063 6 0.6352594793621718 2.0410915293607745 7 0.405192854130711 1.518867513812002 8 0.3059186720879797 1.310557403302358 9 0.23682384514650384 1.141374099835182 >10 1.4368749616707608 14.242954249763166 >50 0.09423174195688963 3.4093110985601425 >100 0.044101174215274215 4.241648578003544 >500 0.003392271506372516 1.2356392951083335 >1k 0.0017546231929513015 1.4861998335060487 >5k 1.1697487953008676E-4 0.405344310992904 >10k+ 2.3394975906017353E-4 1.4251257022716057 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12245 0.7658174608882732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10506 0.6570582477821313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6471 0.40470435193205523 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2610 0.16323263151640613 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 0.1612938531344105 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2211 0.13827867750297854 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.11388759463271095 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1715 0.10725822339104849 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 6.254123812889124E-5 12-13 0.0 0.0 0.0 0.0 1.563530953222281E-4 14-15 0.0 0.0 0.0 0.0 2.1889433345111933E-4 16-17 0.0 0.0 0.0 6.254123812889124E-5 2.5016495251556494E-4 18-19 0.0 0.0 0.0 1.563530953222281E-4 3.1270619064445617E-4 20-21 0.0 0.0 0.0 1.8762371438667372E-4 3.7524742877334744E-4 22-23 0.0 0.0 0.0 3.7524742877334744E-4 3.7524742877334744E-4 24-25 0.0 0.0 0.0 7.192242384822492E-4 3.7524742877334744E-4 26-27 0.0 0.0 0.0 0.0016886134294800633 3.7524742877334744E-4 28-29 0.0 0.0 0.0 0.005472358336277983 3.7524742877334744E-4 30-31 0.0 0.0 0.0 0.016448345627898396 3.7524742877334744E-4 32-33 0.0 0.0 0.0 0.03946352125933037 3.7524742877334744E-4 34-35 0.0 0.0 0.0 0.07336087232518942 3.7524742877334744E-4 36-37 0.0 0.0 0.0 0.12605186544878028 3.7524742877334744E-4 38-39 0.0 0.0 0.0 0.20388443630018543 3.7524742877334744E-4 40-41 0.0 0.0 0.0 0.2849378809152285 3.7524742877334744E-4 42-43 0.0 0.0 0.0 0.36602259614933597 3.7524742877334744E-4 44-45 0.0 0.0 0.0 0.4634618451541485 4.0651804783779305E-4 46-47 0.0 0.0 0.0 0.5768491098818284 4.3778866690223866E-4 48-49 0.0 0.0 0.0 0.6926754828965349 4.3778866690223866E-4 50-51 0.0 0.0 0.0 0.816538405010804 4.3778866690223866E-4 52-53 0.0 0.0 0.0 0.9351791337413107 4.3778866690223866E-4 54-55 0.0 0.0 0.0 1.0619189528095088 5.003299050311299E-4 56-57 0.0 0.0 0.0 1.2036999396477053 5.628711431600211E-4 58-59 0.0 0.0 0.0 1.3620856252091222 5.628711431600211E-4 60-61 0.0 0.0 0.0 1.5266941639643639 5.628711431600211E-4 62-63 0.0 0.0 0.0 1.687800393384388 6.254123812889123E-4 64-65 0.0 0.0 0.0 1.8519086022345985 6.254123812889123E-4 66-67 0.0 0.0 0.0 2.0410333063363657 6.254123812889123E-4 68-69 0.0 0.0 0.0 2.25004612416312 6.254123812889123E-4 70-71 0.0 0.0 0.0 2.469941117424302 6.254123812889123E-4 72-73 0.0 0.0 0.0 2.6843637523492054 6.254123812889123E-4 74-75 0.0 0.0 0.0 2.9031955445621955 6.879536194178037E-4 76-77 0.0 0.0 0.0 3.1346919374962865 6.879536194178037E-4 78-79 0.0 0.0 0.0 3.390704495776903 6.879536194178037E-4 80-81 0.0 0.0 0.0 3.6736097864529427 6.879536194178037E-4 82-83 0.0 0.0 0.0 3.9570466776530773 6.879536194178037E-4 84-85 0.0 0.0 0.0 4.23582424661261 6.879536194178037E-4 86-87 0.0 0.0 0.0 4.522169305385739 6.879536194178037E-4 88-89 0.0 0.0 0.0 4.827745794883501 6.879536194178037E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4725 0.0 54.092434 1 GTATCAA 8240 0.0 40.184784 1 ATCAACG 10700 0.0 30.942167 3 TATCAAC 10735 0.0 30.80089 2 TCAACGC 10855 0.0 30.498434 4 CAACGCA 11145 0.0 29.704845 5 AACGCAG 11390 0.0 29.107592 6 TGGTATC 2070 0.0 25.47468 2 ACGCAGA 13105 0.0 25.262154 7 CGCAGAG 13105 0.0 25.225908 8 TACCTGG 1410 0.0 24.932665 2 GTACATG 8130 0.0 24.483843 1 GTGGTAT 2290 0.0 24.479504 1 TACATGG 8170 0.0 23.666197 2 CATGGGG 3925 0.0 23.47668 4 ATGGGGA 2220 0.0 23.321053 5 GCAGAGT 14185 0.0 23.305288 9 GAGTACT 8700 0.0 22.629757 12-13 ACATGGG 8295 0.0 22.390415 3 GTACCTG 1570 0.0 22.089163 1 >>END_MODULE