FastQCFastQC Report
Thu 26 May 2016
SRR938076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540647
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114520.7433240709909538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91390.5931923406205315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.38509794910839407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25330.16441144532134874No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.1433164118711165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.12884197353449559No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.12300027196366203No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16430.10664350756532806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.054.8737451
GTATCAA77200.043.7426341
TATCAAC98550.034.3181042
ATCAACG99450.033.911873
TCAACGC100400.033.5436134
CAACGCA102150.032.9689525
AACGCAG104300.032.3742376
GTACGCT600.003923993631.7116643
ACGCAGA118600.028.3794867
CGCAGAG119000.028.0816068
GTGTACG1554.4698027E-827.6558381
TATCACG700.00833450627.1814272
GTGGTAT20700.026.0006431
GTACATG77000.025.8560121
TGGTATC19650.025.659822
GCAGAGT127750.025.6364449
TACATGG78050.025.2311882
GTACCTG14250.024.7339131
TACCTGG13800.024.4731332
ACATGGG78950.024.2205623
Produced by FastQC (version 0.11.5)