FastQCFastQC Report
Thu 26 May 2016
SRR938076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540647
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112920.7329388237539164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95910.6225306640651622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62520.4058035357872375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23990.1557138007603299No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.15467527603662617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.13059448400574564No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.11586041448819878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.055.0410581
GTATCAA79350.040.9506231
TATCAAC101050.031.9644722
ATCAACG101550.031.801933
TCAACGC103000.031.39834
CAACGCA104500.030.9930525
AACGCAG107850.030.1184226
TGGTATC19900.028.8819982
ACGCAGA122400.026.3838317
CGCAGAG123100.026.1952238
GTGGTAT22150.026.1660061
GTACATG82250.024.6628531
TACATGG82200.024.1545752
GCAGAGT132200.024.068769
TAAGGTG8450.023.0421735
ACATGGG83350.023.019733
GAGTACT82150.022.66150912-13
GGACCGA4400.021.586076
GTACTTT85000.021.56575414-15
AGGACCG5300.021.5046125
Produced by FastQC (version 0.11.5)