##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938076_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1540647 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48098234053615 33.0 31.0 34.0 31.0 34.0 2 32.688487369267584 34.0 31.0 34.0 31.0 34.0 3 32.73477636343692 34.0 31.0 34.0 31.0 34.0 4 36.21462216847857 37.0 35.0 37.0 35.0 37.0 5 36.18963007100264 37.0 35.0 37.0 35.0 37.0 6 36.2291550238309 37.0 37.0 37.0 35.0 37.0 7 36.184788598556324 37.0 37.0 37.0 35.0 37.0 8 36.169124400333104 37.0 37.0 37.0 35.0 37.0 9 37.841985866976664 39.0 38.0 39.0 35.0 39.0 10-11 37.81868364394958 39.0 38.0 39.0 35.0 39.0 12-13 37.73463681167718 39.0 38.0 39.0 35.0 39.0 14-15 38.9782607566821 40.0 38.0 41.0 35.5 41.0 16-17 38.868570152669626 40.0 38.0 41.0 35.0 41.0 18-19 39.13318657680831 40.0 38.5 41.0 36.0 41.0 20-21 39.11427212073889 40.0 39.0 41.0 36.0 41.0 22-23 39.01739042103739 40.0 38.5 41.0 35.0 41.0 24-25 38.91323807465305 40.0 38.0 41.0 35.0 41.0 26-27 38.736210176633584 40.0 38.0 41.0 35.0 41.0 28-29 38.611272082443286 40.0 38.0 41.0 34.5 41.0 30-31 38.33935125956822 40.0 38.0 41.0 34.0 41.0 32-33 38.09927549918963 40.0 37.0 41.0 34.0 41.0 34-35 37.926073591160076 40.0 37.0 41.0 33.0 41.0 36-37 37.90735353393737 40.0 37.0 41.0 33.0 41.0 38-39 37.75665191312481 40.0 37.0 41.0 33.0 41.0 40-41 37.56363430428904 40.0 37.0 41.0 33.0 41.0 42-43 37.31237265901923 40.0 36.5 41.0 32.5 41.0 44-45 37.00228313169727 40.0 36.0 41.0 31.5 41.0 46-47 36.55244971755373 39.5 35.5 41.0 30.5 41.0 48-49 36.10399754129271 39.0 35.0 41.0 29.5 41.0 50-51 35.70176685509399 39.0 35.0 40.5 28.0 41.0 52-53 35.16698666209716 38.0 34.5 40.0 27.0 41.0 54-55 34.84219032653165 38.0 34.0 40.0 26.0 41.0 56-57 34.48967187162277 38.0 34.0 40.0 25.5 41.0 58-59 34.045626610118994 37.0 33.5 40.0 24.0 41.0 60-61 33.5656860396963 37.0 33.0 39.5 23.0 41.0 62-63 33.09641144272504 36.0 32.5 39.0 22.0 40.5 64-65 32.59938454428561 35.5 32.0 39.0 20.0 40.0 66-67 32.0389200121767 35.0 31.0 38.5 18.0 40.0 68-69 31.465792618296078 35.0 31.0 38.0 14.5 40.0 70-71 30.858835606079783 34.5 30.0 37.0 9.5 39.0 72-73 30.28179881569237 34.0 29.0 36.5 7.0 39.0 74-75 29.682811831652543 34.0 29.0 36.0 4.0 39.0 76-77 28.365023266199202 32.5 27.0 34.5 2.0 36.5 78-79 29.051430665168596 34.0 29.0 35.0 2.0 37.0 80-81 28.730437277325695 34.0 28.5 35.0 2.0 37.0 82-83 28.18000327135288 33.5 27.0 35.0 2.0 36.0 84-85 27.595111015047575 33.0 26.0 35.0 2.0 36.0 86-87 26.95506271066636 33.0 25.5 35.0 2.0 35.0 88-89 26.207012703104603 32.0 24.0 34.5 2.0 35.0 90-91 25.667466330703917 32.0 22.0 34.0 2.0 35.0 92-93 24.85255058426752 31.0 19.0 34.0 2.0 35.0 94-95 24.027468329864014 31.0 11.0 34.0 2.0 35.0 96-97 22.982603737261034 31.0 2.0 34.0 2.0 35.0 98-99 21.53636459227844 30.0 2.0 34.0 2.0 35.0 100-101 17.244580036828683 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06313903073477434 1101 2 0.12288152611652237 1101 3 0.07682155567740523 1101 4 0.04700644003552412 1101 5 0.1736719971993992 1101 6 0.09831411338223717 1101 7 0.06562709302389891 1101 8 0.05740593622744683 1101 9 -0.020843992337681527 1101 10-11 0.015402908273962623 1101 12-13 0.12071572712842737 1101 14-15 0.1332069149215016 1101 16-17 0.21382007955784132 1101 18-19 0.0646325788133808 1101 20-21 0.1456068302640432 1101 22-23 0.14205335706451194 1101 24-25 0.17234495051528853 1101 26-27 0.21612167692537554 1101 28-29 0.2613063534072353 1101 30-31 0.3489697113294241 1101 32-33 0.1509068248916563 1101 34-35 0.4017719144646321 1101 36-37 0.43409606251061916 1101 38-39 0.41237704252491625 1101 40-41 0.3283232277431125 1101 42-43 0.3401204364254866 1101 44-45 0.365218440642181 1101 46-47 0.41072087971148363 1101 48-49 0.2914148826627283 1101 50-51 0.15426375694833183 1101 52-53 0.18638986963791382 1101 54-55 -0.062048587324774473 1101 56-57 0.05980992231906157 1101 58-59 0.20684791069911057 1101 60-61 0.1526118725657497 1101 62-63 0.2731355453876887 1101 64-65 0.2617849222860329 1101 66-67 -0.053822349201645636 1101 68-69 0.1849488724673698 1101 70-71 0.20889226825710594 1101 72-73 -0.11870867926653617 1101 74-75 -0.14147863986384124 1101 76-77 -0.26728289498533897 1101 78-79 -0.292519045461642 1101 80-81 -0.3394457641875839 1101 82-83 -0.37695758329675755 1101 84-85 -0.3953739332854376 1101 86-87 -0.6197022634288025 1101 88-89 -0.5412161919774938 1101 90-91 -0.611122647013179 1101 92-93 -0.8033514950912028 1101 94-95 -0.8805006604220758 1101 96-97 -1.27080229213459 1101 98-99 -1.5005834129787239 1101 100-101 -2.010091921823564 1102 1 0.20498006706955607 1102 2 0.13019670575017983 1102 3 0.16979068449589363 1102 4 0.12370295069907655 1102 5 0.1000422023095382 1102 6 0.05594182034050732 1102 7 0.1175922129077378 1102 8 0.10319628987748786 1102 9 0.1743627738325273 1102 10-11 0.23266199461841097 1102 12-13 0.24427119907250727 1102 14-15 0.3204572838865758 1102 16-17 0.21944954588993681 1102 18-19 0.3349974634074684 1102 20-21 0.1784101433981462 1102 22-23 0.30209808331495225 1102 24-25 0.2670970351348103 1102 26-27 0.24905349853970193 1102 28-29 0.22088712429913926 1102 30-31 0.1863587899633714 1102 32-33 0.0428423215835565 1102 34-35 0.16794715763712276 1102 36-37 0.30672110656121276 1102 38-39 0.25140387562551325 1102 40-41 0.2330274330175257 1102 42-43 0.28013214791997143 1102 44-45 0.2003643176396963 1102 46-47 0.32504281862623685 1102 48-49 0.29868236842743556 1102 50-51 0.3534206353316165 1102 52-53 0.415998488449965 1102 54-55 0.4447938930743689 1102 56-57 0.5053379596898537 1102 58-59 0.38513077243723615 1102 60-61 0.3119920642421121 1102 62-63 0.3713046307517587 1102 64-65 0.032884292461993425 1102 66-67 0.10530427007144993 1102 68-69 0.06492274682581822 1102 70-71 -0.06120526570490625 1102 72-73 -0.1547896210614681 1102 74-75 -0.05152482342694498 1102 76-77 -0.2358807217347092 1102 78-79 -0.16255669712152354 1102 80-81 -0.15990007478595558 1102 82-83 -0.1864423040359071 1102 84-85 -0.23952096818409885 1102 86-87 -0.18081639220755008 1102 88-89 -0.3554917618529849 1102 90-91 -0.6812087874398252 1102 92-93 -0.6997580270315211 1102 94-95 -0.5600611879162685 1102 96-97 -0.6594938108398587 1102 98-99 -0.8323615728962679 1102 100-101 -1.4323021616972298 1103 1 0.1359387421209064 1103 2 0.04666864201694665 1103 3 0.09781612325330968 1103 4 0.03263378306252207 1103 5 0.04900178560151858 1103 6 0.07664088493285703 1103 7 0.05434919237608682 1103 8 0.0825378254457334 1103 9 0.20356531650529774 1103 10-11 0.1577135969212975 1103 12-13 0.19238895557780467 1103 14-15 0.19778139246243143 1103 16-17 0.17120352295135177 1103 18-19 0.2577892407220972 1103 20-21 0.27942484667111955 1103 22-23 0.2729373938941535 1103 24-25 0.2993596269488208 1103 26-27 0.3042098191827236 1103 28-29 0.2803054593316574 1103 30-31 0.24878880480999754 1103 32-33 0.27814606592083635 1103 34-35 0.12613024531442107 1103 36-37 0.0690026551415599 1103 38-39 0.18881998115786303 1103 40-41 0.3249477003169545 1103 42-43 0.20709270962569803 1103 44-45 0.23001722135315106 1103 46-47 0.03534638753072983 1103 48-49 -0.023354095529150243 1103 50-51 -0.12608305026073907 1103 52-53 -0.12316262200953787 1103 54-55 0.03492750268929257 1103 56-57 0.03329117026032691 1103 58-59 -0.03018982347287391 1103 60-61 -0.11375650748433941 1103 62-63 -0.23229870868663482 1103 64-65 -0.11109766101723295 1103 66-67 -0.08124720241116279 1103 68-69 0.04420438462627274 1103 70-71 -0.187421892122277 1103 72-73 -0.27934765573300524 1103 74-75 -0.4259638144177167 1103 76-77 -0.5659587742903653 1103 78-79 -0.4857575335850761 1103 80-81 -0.43425515877816423 1103 82-83 -0.47622820585569414 1103 84-85 -0.4776772497306787 1103 86-87 -0.7577875300044958 1103 88-89 -0.7866391524656287 1103 90-91 -1.1998936329537102 1103 92-93 -1.0579248746445842 1103 94-95 -0.9556267982770237 1103 96-97 -1.1686178197698887 1103 98-99 -1.3321262423357716 1103 100-101 -1.8099402949720194 1104 1 0.022683027560645996 1104 2 0.0822941099239074 1104 3 0.009976302088190891 1104 4 0.02834983488867948 1104 5 -0.11271689941140295 1104 6 -0.029659927492261318 1104 7 0.001189224846321224 1104 8 0.036410684786325476 1104 9 0.035208208956731823 1104 10-11 0.021001102861262666 1104 12-13 0.06704462864195904 1104 14-15 -0.005360026111958405 1104 16-17 0.10215742688497187 1104 18-19 0.00953943983653005 1104 20-21 0.013242078268220325 1104 22-23 0.029057114971408282 1104 24-25 -0.012620894735768218 1104 26-27 -0.03629139462649533 1104 28-29 -0.09949946111339614 1104 30-31 -0.09990430654779914 1104 32-33 -0.03184976534486594 1104 34-35 -0.035334825759626654 1104 36-37 -0.04232262947495258 1104 38-39 0.005469772099665704 1104 40-41 0.013299434405247723 1104 42-43 0.05330394206992395 1104 44-45 0.02258755201218321 1104 46-47 -0.0017125608582446716 1104 48-49 0.016288081735758908 1104 50-51 -0.1532732850962546 1104 52-53 -0.11957425966239299 1104 54-55 -0.10644015768507131 1104 56-57 -0.2981267922038455 1104 58-59 -0.20441949204894172 1104 60-61 -0.5925191487408554 1104 62-63 -0.5188176816864285 1104 64-65 -0.41192514521477364 1104 66-67 -0.5465895788383079 1104 68-69 -0.6620937028234444 1104 70-71 -0.47567959065585796 1104 72-73 -0.7558581287218047 1104 74-75 -0.6362059452146269 1104 76-77 -0.8509555855910769 1104 78-79 -0.7882938642001278 1104 80-81 -1.067536813192003 1104 82-83 -0.9900180155950409 1104 84-85 -1.012062375763179 1104 86-87 -1.1513091161122837 1104 88-89 -1.1076735244448521 1104 90-91 -1.2235806011651995 1104 92-93 -1.341455457951863 1104 94-95 -1.260108139844565 1104 96-97 -1.6343142039665643 1104 98-99 -1.557172638627513 1104 100-101 -1.7440341354758608 1105 1 0.06679950161457526 1105 2 0.1024998656456404 1105 3 0.029413872901692173 1105 4 -0.01575440259149019 1105 5 0.05171908517957746 1105 6 0.03966479491879227 1105 7 -0.014595955303235542 1105 8 -0.009768784962140842 1105 9 0.06341623071064362 1105 10-11 0.07999770381299243 1105 12-13 0.04236504559187182 1105 14-15 0.11355635730068059 1105 16-17 0.006615618591290229 1105 18-19 0.10037855155191977 1105 20-21 0.21314214624918293 1105 22-23 0.18408929262833595 1105 24-25 0.11043416984614396 1105 26-27 0.16082303999848335 1105 28-29 0.1180733918211132 1105 30-31 0.15889288357229958 1105 32-33 0.17831202935261814 1105 34-35 0.11893052660837355 1105 36-37 0.10765244417109443 1105 38-39 0.20272152827460133 1105 40-41 -3.4343892355792605E-4 1105 42-43 -0.03320546196361107 1105 44-45 -0.03638502899846685 1105 46-47 0.11735904575857603 1105 48-49 0.19500596072964527 1105 50-51 0.04914479784051906 1105 52-53 -0.08011198909814965 1105 54-55 0.017284746008563445 1105 56-57 0.07648526308733494 1105 58-59 0.11224196274372389 1105 60-61 0.06842352064505519 1105 62-63 0.04957048962560151 1105 64-65 -0.08811594512413023 1105 66-67 -0.08181119678528148 1105 68-69 0.1073169022071454 1105 70-71 0.030739851900225545 1105 72-73 -0.017080426478432997 1105 74-75 -0.16457281582419014 1105 76-77 0.03634783611751047 1105 78-79 -0.1374298261307878 1105 80-81 -0.20949285216775948 1105 82-83 -0.3457345349696155 1105 84-85 -0.4029191625295532 1105 86-87 -0.19777909118592873 1105 88-89 -0.6943648908622535 1105 90-91 -0.7828500323911207 1105 92-93 -0.6407715694406448 1105 94-95 -0.33041020193384796 1105 96-97 -0.7018977072523107 1105 98-99 -0.9809068330530728 1105 100-101 -1.5157982415261664 1106 1 0.26330170425774924 1106 2 0.23742497577779886 1106 3 0.24353134720418979 1106 4 0.15529405555829356 1106 5 0.20579838033376063 1106 6 0.22628740872202258 1106 7 0.20187981562187218 1106 8 0.19688319741671023 1106 9 0.2103560251306149 1106 10-11 0.1815043117425148 1106 12-13 0.23400528054047953 1106 14-15 0.294267076830792 1106 16-17 0.3083219328350495 1106 18-19 0.28987781733752627 1106 20-21 0.3455459641640104 1106 22-23 0.2837089695740005 1106 24-25 0.2816470920194192 1106 26-27 0.3148729665770347 1106 28-29 0.3314170041559237 1106 30-31 0.3890485370231076 1106 32-33 0.44758075181957935 1106 34-35 0.3076221565643209 1106 36-37 0.24226331054407524 1106 38-39 0.27715030947871355 1106 40-41 0.289189600724022 1106 42-43 0.13109997122288064 1106 44-45 0.06736240125117376 1106 46-47 0.1437452425280341 1106 48-49 0.1908444335637114 1106 50-51 0.15585992265696547 1106 52-53 0.2153520343967088 1106 54-55 0.05278254336538879 1106 56-57 -0.2013135621696449 1106 58-59 -0.03078153211677659 1106 60-61 -0.1604751577690422 1106 62-63 -0.18404037674737594 1106 64-65 -0.0828149172239776 1106 66-67 -0.21702265052977765 1106 68-69 -0.2334804546210485 1106 70-71 -0.813420206836927 1106 72-73 -0.5767896775797539 1106 74-75 -0.30859484225590705 1106 76-77 -0.4468239700498877 1106 78-79 -0.49114641937601533 1106 80-81 -0.32428874056717305 1106 82-83 -0.2688637567023662 1106 84-85 -0.5812789275809465 1106 86-87 -0.5042930412593485 1106 88-89 -0.5361372844011676 1106 90-91 -0.7679865962677717 1106 92-93 -0.7817642272967404 1106 94-95 -1.0104527863685036 1106 96-97 -1.143671569220004 1106 98-99 -1.0022827508210632 1106 100-101 -1.425247580733215 1107 1 -0.01627810192382384 1107 2 0.02118422431070144 1107 3 0.06796554157881474 1107 4 -0.018179814734637034 1107 5 0.012055233149020239 1107 6 0.009680878553695038 1107 7 -0.029939742999253838 1107 8 -0.054316432730537656 1107 9 -0.0743473398563026 1107 10-11 0.02219794506751782 1107 12-13 -0.07539168943024066 1107 14-15 -0.14564026513598094 1107 16-17 -0.12660754184296508 1107 18-19 -0.016439703373698933 1107 20-21 -0.09698692492090544 1107 22-23 -0.073225946615743 1107 24-25 -0.020972344025985024 1107 26-27 -0.046126698642453334 1107 28-29 0.010192008628507665 1107 30-31 0.08399220001781771 1107 32-33 -0.18538157740250227 1107 34-35 -0.0834727705367797 1107 36-37 -0.10531900737696276 1107 38-39 -0.1853476313554765 1107 40-41 -0.3364536118226269 1107 42-43 -0.17191314189927454 1107 44-45 -0.2731948400157478 1107 46-47 -0.16226982436607074 1107 48-49 -0.12170815265998414 1107 50-51 -0.10657655318480863 1107 52-53 -0.25852091551551837 1107 54-55 -0.2908785028856897 1107 56-57 -0.16466351053550454 1107 58-59 -0.16356141481961117 1107 60-61 -0.3605109385427241 1107 62-63 -0.3911424174914018 1107 64-65 -0.542993307408004 1107 66-67 -0.40510552730398075 1107 68-69 -0.490508188263302 1107 70-71 -0.6163735377258313 1107 72-73 -0.8775846484325918 1107 74-75 -0.6637819030779077 1107 76-77 -0.7965150508949534 1107 78-79 -0.804955760992069 1107 80-81 -0.5990593982071637 1107 82-83 -0.652802086236207 1107 84-85 -0.605492946204663 1107 86-87 -0.9390223561638145 1107 88-89 -1.0732828643242556 1107 90-91 -1.2311837678153275 1107 92-93 -1.176168433131842 1107 94-95 -1.1843880869358578 1107 96-97 -1.605876798526939 1107 98-99 -1.3215739018106127 1107 100-101 -1.4868657927927522 1108 1 -0.060646339199301735 1108 2 -0.06461922471940795 1108 3 -0.05923124977933725 1108 4 -0.0406831877500764 1108 5 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-0.09153384909394191 2316 52-53 0.016268560584727254 2316 54-55 -0.11231285793787293 2316 56-57 -0.02132587151449883 2316 58-59 -0.1967791789053308 2316 60-61 0.1981839152891709 2316 62-63 0.1686254579131301 2316 64-65 0.30398412534803043 2316 66-67 0.3437025452865967 2316 68-69 0.4627841326511195 2316 70-71 0.5538709301243294 2316 72-73 0.8653804614708278 2316 74-75 1.0449437451342298 2316 76-77 1.070466934073817 2316 78-79 1.0912741907825314 2316 80-81 1.105218916683544 2316 82-83 1.3223775157703415 2316 84-85 1.3922978917340139 2316 86-87 1.5408650243884203 2316 88-89 1.7162566736197782 2316 90-91 1.7067222015764614 2316 92-93 1.8533552804536448 2316 94-95 2.370318480236694 2316 96-97 2.7683616303093537 2316 98-99 3.054757014727052 2316 100-101 3.428588650651424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 12.0 5 34.0 6 66.0 7 167.0 8 414.0 9 759.0 10 1282.0 11 2124.0 12 3451.0 13 5274.0 14 6874.0 15 9578.0 16 12662.0 17 18187.0 18 18512.0 19 13713.0 20 12570.0 21 13131.0 22 14133.0 23 15416.0 24 16968.0 25 19545.0 26 22947.0 27 26682.0 28 32232.0 29 38730.0 30 46466.0 31 56495.0 32 69932.0 33 90450.0 34 122422.0 35 176329.0 36 237203.0 37 283553.0 38 145471.0 39 6855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33091076213623 18.646657734571363 14.556588978209842 22.465842525082568 2 16.074090134817922 19.950170340056918 39.87099856665074 24.10474095847442 3 19.415007734506805 24.490311256287736 29.614495043489153 26.480185965716302 4 13.083853731581602 16.588615042900805 35.63496375224175 34.69256747327584 5 13.621431738034612 37.32308385330426 33.60129713085093 15.454187277810197 6 32.57593361642246 35.477359144553304 16.796008365452394 15.150698873571844 7 29.05110645073141 33.12446004827842 19.661804423725876 18.16262907726429 8 27.17656932444616 34.57625270422102 18.918934707301542 19.32824326403128 9 27.14878878808708 16.327101535913158 19.75118245775963 36.772927218240135 10-11 25.596421503433298 26.60706832908512 25.948351569178403 21.84815859830318 12-13 26.532334305770068 24.007395668594338 27.05290006078676 22.40736996484883 14-15 23.091629685450332 25.04444561278476 25.9062913178684 25.957633383896507 16-17 22.10283930202858 28.53468518100814 26.61355991061967 22.7489156063436 18-19 22.132649464802775 26.242935597836492 29.81847885985563 21.805936077505102 20-21 22.71062092744152 25.32452274920861 30.270821284823846 21.69403503852602 22-23 22.578923010916842 25.29771582977801 30.254367158732663 21.868994000572485 24-25 22.280356251505097 25.600562639028894 30.27813243826906 21.840948671196944 26-27 21.681587421122337 26.048228985431976 30.123802228050582 22.1463813653951 28-29 21.770701885577488 26.217563237730246 30.197379353679498 21.814355523012768 30-31 21.792971927043677 25.864878004768293 30.085168503297105 22.25698156489092 32-33 21.589501034305716 25.972270091721207 29.996942842844597 22.44128603112848 34-35 22.247964216824336 26.048125268378687 29.795543721017943 21.908366793779035 36-37 22.425994752809558 25.673716145431037 29.688913784844424 22.21137531691498 38-39 21.93871093164291 25.924882562065065 29.852245974136064 22.28416053215596 40-41 22.195818539409714 25.82220678822025 29.8607369808063 22.12123769156373 42-43 22.055303128651175 25.8155588731663 29.677008957549006 22.45212904063352 44-45 21.916610981169267 26.15642199557134 29.665370998473694 22.2615960247857 46-47 22.438863228623873 26.4395603397021 28.948576050015447 22.17300038165858 48-49 22.263182952749045 26.546220699185497 28.659601833139636 22.53099451492582 50-51 22.41852319569979 26.688418264201747 28.613639114967675 22.279419425130786 52-53 22.729181772565372 26.569688149337267 28.40384988636902 22.297280191728337 54-55 22.22965115429917 26.772490000963955 28.479669411080067 22.518189433656804 56-57 22.30191658135327 26.96622758129549 28.330030076623004 22.40182576072823 58-59 22.332500484484957 26.530785813501375 28.25636379512892 22.88034990688475 60-61 22.107782013525977 26.725555322600226 28.693404024819046 22.47325863905475 62-63 22.138564971766083 26.70760335664844 28.79223239453993 22.361599277045546 64-65 21.91823954492839 26.743896920722783 28.58613322218825 22.751730312160586 66-67 21.853282530013136 26.957308917099247 28.379977156033252 22.809431396854364 68-69 22.14671195983867 26.64181255794747 28.52333611221487 22.688139369998986 70-71 22.03876385675446 26.78472660418955 28.240967953861457 22.93554158519453 72-73 22.075847020712903 26.900776113124987 28.03386946902152 22.98950739714059 74-75 21.931696645205896 27.2481145411198 27.925370397118943 22.89481841655536 76-77 22.07188359693898 26.95350856549295 28.17233236222581 22.802275475342253 78-79 22.034522787740755 27.120813526045634 28.035725183702993 22.808938502510617 80-81 22.028540037358788 27.209077763730814 27.960667784292752 22.801714414617642 82-83 21.99006386771562 27.27466039536106 27.837629976304047 22.89764576061927 84-85 21.948667775960693 27.15242751663779 27.84553069851115 23.053374008890366 86-87 22.085413571131067 27.088664796549256 27.838021125336283 22.9879005069834 88-89 21.94327531280899 27.26412809830704 27.493496468584066 23.29910012029991 90-91 21.89844406859992 27.270513915084386 27.636302252578343 23.194739763737353 92-93 21.93013209303775 26.870960956398783 27.751548746395134 23.44735820416834 94-95 21.69618774039632 26.7166528717724 27.936673320674487 23.65048606715679 96-97 21.978590896830376 26.557181339239083 27.75289458488418 23.711333179046356 98-99 21.639289137413893 26.79746687123673 27.483655113564627 24.07958887778475 100-101 22.174884792178005 26.138202647621483 27.512127737058066 24.174784823142442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2439.0 1 1640.0 2 1374.0 3 1793.5 4 1240.0 5 915.0 6 1756.5 7 3694.5 8 5704.0 9 6324.5 10 4729.0 11 2977.5 12 2623.5 13 2656.5 14 2714.5 15 2755.5 16 2810.5 17 2919.0 18 3017.5 19 3114.0 20 3354.0 21 3821.5 22 4448.0 23 5156.0 24 6008.0 25 7131.0 26 8659.5 27 10627.5 28 12115.5 29 13663.5 30 15975.5 31 18473.0 32 22098.0 33 24259.5 34 25230.5 35 27082.0 36 29656.5 37 32752.0 38 34907.5 39 36692.0 40 38563.0 41 41433.0 42 43977.0 43 46282.5 44 48874.0 45 51857.0 46 53234.0 47 54475.5 48 56851.0 49 56807.0 50 56067.0 51 55504.0 52 55252.0 53 55857.5 54 56694.5 55 53635.5 56 48465.5 57 44996.0 58 42546.5 59 38658.5 60 32282.0 61 26516.0 62 21849.0 63 16623.5 64 11846.0 65 8823.0 66 6583.0 67 5083.5 68 3880.0 69 2702.0 70 1862.0 71 1298.0 72 946.0 73 723.0 74 529.0 75 371.5 76 243.0 77 142.0 78 90.0 79 60.0 80 29.0 81 14.0 82 10.5 83 6.5 84 3.0 85 3.5 86 2.5 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00876255235625033 2 0.012916651251065298 3 0.00960635369425962 4 0.0 5 0.0010385247237037426 6 0.0016226948807870978 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010060708260880007 14-15 0.0 16-17 0.0025638579116436144 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0032453897615741956 26-27 0.018628537231435885 28-29 0.007204765270694715 30-31 0.003505020942500131 32-33 0.0 34-35 0.009508992001412394 36-37 8.43801338009291E-4 38-39 0.00298575858064826 40-41 0.002401588423564905 42-43 0.003050666375879744 44-45 3.245389761574196E-5 46-47 1.9472338569445172E-4 48-49 7.46439645162065E-4 50-51 0.004575999563819617 52-53 0.008697644561018845 54-55 0.008016112711088264 56-57 0.01609713321740801 58-59 0.022815090023866596 60-61 0.0036348365329630992 62-63 0.019537246364676658 64-65 0.020640678883611888 66-67 0.01275438176298659 68-69 0.029792678011251118 70-71 0.01577259424125059 72-73 0.005549616492291874 74-75 0.015545416957940398 76-77 0.020088962624144273 78-79 0.025249132345047245 80-81 0.011650949244051363 82-83 0.03313542946567254 84-85 0.021289756835926724 86-87 0.029760224113635377 88-89 0.021419572426389692 90-91 0.026742011635371377 92-93 0.023950976440417566 94-95 0.01440953054138943 96-97 0.01476652341516259 98-99 0.011715857039282846 100-101 0.011034325189352266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1540647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.18882512822177 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1756993414346 42.36249300889441 2 12.286554630437383 13.315879205942297 3 3.7816702572168173 6.147728047827586 4 1.7090116908326538 3.704373426264692 5 0.9358266158562207 2.5355672418484154 6 0.5881575459781467 1.9122939844112306 7 0.4345154641350006 1.6482117751063765 8 0.31786952140898395 1.377998072737838 9 0.23947266285995134 1.167906802563676 >10 1.3913092336096182 13.905914613931433 >50 0.09197805971125171 3.411504872168091 >100 0.042422577930988116 4.104475187052419 >500 0.003595042557734118 1.3204947930584636 >1k 0.0015578517750181181 1.3230922748249911 >5k 2.3966950384894125E-4 1.0287975906774154 >10k+ 1.1983475192447062E-4 0.7332691026907387 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11292 0.7329388237539164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9591 0.6225306640651622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6252 0.4058035357872375 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2399 0.1557138007603299 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2383 0.15467527603662617 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 0.13059448400574564 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1785 0.11586041448819878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.245389761574196E-5 14-15 0.0 0.0 0.0 0.0 6.490779523148391E-5 16-17 0.0 0.0 0.0 0.0 6.490779523148391E-5 18-19 0.0 0.0 0.0 3.245389761574196E-5 6.490779523148391E-5 20-21 0.0 0.0 0.0 1.9472338569445174E-4 6.490779523148391E-5 22-23 0.0 0.0 0.0 6.166240546990972E-4 6.490779523148391E-5 24-25 0.0 0.0 0.0 7.78893542777807E-4 6.490779523148391E-5 26-27 0.0 0.0 0.0 0.0019472338569445173 9.736169284722587E-5 28-29 0.0 0.0 0.0 0.0052250775161344545 1.2981559046296783E-4 30-31 0.0 0.0 0.0 0.014831431210394076 1.2981559046296783E-4 32-33 0.0 0.0 0.0 0.036900081589098604 1.2981559046296783E-4 34-35 0.0 0.0 0.0 0.07126875916416933 1.2981559046296783E-4 36-37 0.0 0.0 0.0 0.12595357664669454 1.2981559046296783E-4 38-39 0.0 0.0 0.0 0.20562789529334105 1.2981559046296783E-4 40-41 0.0 0.0 0.0 0.28767134846593667 1.2981559046296783E-4 42-43 0.0 0.0 0.0 0.36763775219112493 1.2981559046296783E-4 44-45 0.0 0.0 0.0 0.4630197572837905 1.2981559046296783E-4 46-47 0.0 0.0 0.0 0.579431888031457 1.2981559046296783E-4 48-49 0.0 0.0 0.0 0.6998358481858595 1.2981559046296783E-4 50-51 0.0 0.0 0.0 0.8300408854202164 1.2981559046296783E-4 52-53 0.0 0.0 0.0 0.9489844201819106 1.9472338569445174E-4 54-55 0.0 0.0 0.0 1.0759116137570772 2.5963118092593566E-4 56-57 0.0 0.0 0.0 1.2214997984612959 2.920850785416776E-4 58-59 0.0 0.0 0.0 1.3818220526830611 3.245389761574196E-4 60-61 0.0 0.0 0.0 1.548862263711285 3.245389761574196E-4 62-63 0.0 0.0 0.0 1.715026219503884 3.245389761574196E-4 64-65 0.0 0.0 0.0 1.8800542888799314 3.245389761574196E-4 66-67 0.0 0.0 0.0 2.068254441153619 3.245389761574196E-4 68-69 0.0 0.0 0.0 2.276056747587215 3.245389761574196E-4 70-71 0.0 0.0 0.0 2.49521791818632 3.245389761574196E-4 72-73 0.0 0.0 0.0 2.711360876307162 3.894467713889035E-4 74-75 0.0 0.0 0.0 2.936493564067564 4.543545666203874E-4 76-77 0.0 0.0 0.0 3.173050023788707 4.543545666203874E-4 78-79 0.0 0.0 0.0 3.4413463953780457 4.868084642361294E-4 80-81 0.0 0.0 0.0 3.721001631132894 5.517162594676132E-4 82-83 0.0 0.0 0.0 4.00471360408971 5.841701570833553E-4 84-85 0.0 0.0 0.0 4.2844662015374055 5.841701570833553E-4 86-87 0.0 0.0 0.0 4.566328302330125 6.166240546990972E-4 88-89 0.0 0.0 0.0 4.869609975549233 6.490779523148391E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4445 0.0 55.041058 1 GTATCAA 7935 0.0 40.950623 1 TATCAAC 10105 0.0 31.964472 2 ATCAACG 10155 0.0 31.80193 3 TCAACGC 10300 0.0 31.3983 4 CAACGCA 10450 0.0 30.993052 5 AACGCAG 10785 0.0 30.118422 6 TGGTATC 1990 0.0 28.881998 2 ACGCAGA 12240 0.0 26.383831 7 CGCAGAG 12310 0.0 26.195223 8 GTGGTAT 2215 0.0 26.166006 1 GTACATG 8225 0.0 24.662853 1 TACATGG 8220 0.0 24.154575 2 GCAGAGT 13220 0.0 24.06876 9 TAAGGTG 845 0.0 23.042173 5 ACATGGG 8335 0.0 23.01973 3 GAGTACT 8215 0.0 22.661509 12-13 GGACCGA 440 0.0 21.58607 6 GTACTTT 8500 0.0 21.565754 14-15 AGGACCG 530 0.0 21.504612 5 >>END_MODULE