FastQCFastQC Report
Thu 26 May 2016
SRR938075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855117
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89230.4809939211381277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72920.39307493813058686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.2387450495036162No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24820.13379210044433854No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24230.13061170804860286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22370.12058538625865647No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21560.1162190848340024No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21500.11589565509884284No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19440.10479123419169789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.033.9753531
GTATCAA97350.032.043371
ATCAACG117250.026.0621243
TATCAAC118000.026.0173022
TCAACGC117250.025.981064
CAACGCA117800.025.8194165
GTGTAAG7050.025.032771
AACGCAG121700.025.0270066
GTACATG68250.022.7131331
ACGCAGA134300.022.6027287
GAGTACT77350.022.53754612-13
CGCAGAG135600.022.2815368
TACATGG68600.021.9607332
GTACTTT81650.021.12049314-15
GCAGAGT141600.020.7659459
AGAGTAC109600.020.69603510-11
ACATGGG70350.020.4011463
AGTACTT80550.019.54874412-13
GTGTAGC12000.019.4765111
CGCCGTA2001.8189894E-1219.01467394-95
Produced by FastQC (version 0.11.5)