##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938075_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855117 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733465867651475 33.0 31.0 34.0 30.0 34.0 2 31.89496673255649 33.0 31.0 34.0 30.0 34.0 3 31.978206226345833 34.0 31.0 34.0 30.0 34.0 4 35.5126986599767 37.0 35.0 37.0 33.0 37.0 5 35.525784627061256 37.0 35.0 37.0 33.0 37.0 6 35.55265463040875 37.0 35.0 37.0 33.0 37.0 7 35.47907167041216 37.0 35.0 37.0 33.0 37.0 8 35.44402212906248 37.0 35.0 37.0 33.0 37.0 9 37.053351351963244 39.0 37.0 39.0 33.0 39.0 10-11 36.940489467780196 39.0 37.0 39.0 33.0 39.0 12-13 36.80466919337163 39.0 37.0 39.0 33.0 39.0 14-15 37.912613867481134 40.0 38.0 41.0 33.0 41.0 16-17 37.7306231898042 40.0 38.0 41.0 32.0 41.0 18-19 37.92825412089911 40.0 38.0 41.0 33.0 41.0 20-21 37.914658751981676 40.0 38.0 41.0 33.0 41.0 22-23 37.79510537610297 40.0 38.0 41.0 32.0 41.0 24-25 37.649285193332815 40.0 38.0 41.0 32.0 41.0 26-27 37.430522441441695 40.0 37.0 41.0 32.0 41.0 28-29 37.21084869579654 40.0 37.0 41.0 31.0 41.0 30-31 36.93358639913278 39.5 36.5 41.0 30.5 41.0 32-33 36.58323329471942 39.0 35.5 41.0 30.0 41.0 34-35 36.2660435972502 39.0 35.0 40.5 30.0 41.0 36-37 35.93126875555558 39.0 35.0 40.0 28.5 41.0 38-39 35.57369831660213 38.0 35.0 40.0 27.0 41.0 40-41 35.38349009792913 38.0 35.0 40.0 26.5 41.0 42-43 35.09195511657755 38.0 34.5 40.0 25.0 41.0 44-45 34.692301078584265 38.0 34.0 40.0 24.0 41.0 46-47 34.18196399472379 38.0 33.0 40.0 22.5 41.0 48-49 33.6715500963012 38.0 33.0 40.0 19.0 41.0 50-51 32.83318545407109 37.5 32.0 39.5 15.5 40.5 52-53 32.800651117961834 37.0 32.0 39.5 14.0 40.5 54-55 32.755863107286494 37.5 32.0 40.0 9.5 41.0 56-57 32.253559478997815 37.0 31.0 40.0 7.5 41.0 58-59 31.774234994342674 36.5 31.0 40.0 2.0 41.0 60-61 31.172064349580104 36.0 29.5 39.0 2.0 41.0 62-63 30.52813784790932 35.0 28.5 39.0 2.0 40.5 64-65 29.90260210002927 35.0 28.0 38.5 2.0 40.0 66-67 29.181951866108715 34.5 26.5 38.0 2.0 40.0 68-69 28.457108365671814 34.0 26.0 37.5 2.0 40.0 70-71 27.599242797085036 34.0 24.0 37.0 2.0 39.0 72-73 26.783752183824525 33.0 21.5 36.0 2.0 39.0 74-75 25.945136883549665 32.5 18.5 35.5 2.0 38.5 76-77 25.08904748325847 32.0 10.5 35.0 2.0 37.5 78-79 24.226239099744113 31.0 4.5 35.0 2.0 37.0 80-81 23.402930920260015 31.0 2.0 35.0 2.0 36.0 82-83 22.55982938003371 30.0 2.0 34.0 2.0 36.0 84-85 21.737330583461855 29.0 2.0 34.0 2.0 35.0 86-87 20.970244464365322 29.0 2.0 34.0 2.0 35.0 88-89 20.115974356334398 27.5 2.0 34.0 2.0 35.0 90-91 19.243841763080173 26.0 2.0 34.0 2.0 35.0 92-93 18.340473134578573 24.5 2.0 33.0 2.0 35.0 94-95 17.39991628560355 21.0 2.0 33.0 2.0 35.0 96-97 16.288980425493378 8.5 2.0 32.0 2.0 34.5 98-99 15.001655960244017 2.0 2.0 32.0 2.0 34.0 100-101 12.120501025002735 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5681975760903413 1101 2 0.4161796951925112 1101 3 0.5114828070375665 1101 4 0.4397917900803918 1101 5 0.4221137123835561 1101 6 0.37020859756276536 1101 7 0.3041485942482751 1101 8 0.37038690767628424 1101 9 0.45031314669149225 1101 10-11 0.48010538788113166 1101 12-13 0.4915523117574381 1101 14-15 0.6691924499008408 1101 16-17 0.838322307626413 1101 18-19 0.7498431862857657 1101 20-21 0.7040931788112417 1101 22-23 0.785502390423936 1101 24-25 0.8714364046327674 1101 26-27 0.9100858178512325 1101 28-29 1.0635543767948903 1101 30-31 1.1887682024298059 1101 32-33 1.2446483125340748 1101 34-35 1.2013072641807625 1101 36-37 1.2157059760409226 1101 38-39 1.530061621342469 1101 40-41 1.5880575546377713 1101 42-43 1.59456379411494 1101 44-45 1.754733979004115 1101 46-47 1.8923537038135905 1101 48-49 2.107748065462225 1101 50-51 2.145233470232853 1101 52-53 2.2364704150593155 1101 54-55 2.3505605616028404 1101 56-57 2.4512167171242183 1101 58-59 2.772047952553894 1101 60-61 2.975343817510499 1101 62-63 2.932831384614108 1101 64-65 3.0446929452571716 1101 66-67 3.2816832618147167 1101 68-69 3.5315766109673277 1101 70-71 3.7600232088379784 1101 72-73 4.088045773681994 1101 74-75 4.169282642727225 1101 76-77 4.614265048932712 1101 78-79 4.799470518853575 1101 80-81 5.004455589642454 1101 82-83 5.389299738466022 1101 84-85 5.697732133425625 1101 86-87 5.725725805609301 1101 88-89 5.731576155593775 1101 90-91 5.67472199703376 1101 92-93 5.91015854872612 1101 94-95 5.8597771461874295 1101 96-97 5.9606029476518 1101 98-99 5.846037121440133 1101 100-101 4.893579249584391 1102 1 0.4364732184457907 1102 2 0.45032900023355893 1102 3 0.3987112692371326 1102 4 0.346999951773185 1102 5 0.38235954114804116 1102 6 0.3456094214373522 1102 7 0.35524659976052675 1102 8 0.33865365929884206 1102 9 0.5619985332901933 1102 10-11 0.5152411938260641 1102 12-13 0.5164997082544929 1102 14-15 0.7772500470320978 1102 16-17 0.8359206984037613 1102 18-19 0.7347352379705612 1102 20-21 0.5805468136392022 1102 22-23 0.7401718774286437 1102 24-25 0.6051263551410173 1102 26-27 0.7083650905892824 1102 28-29 0.7762555296265887 1102 30-31 0.8210400810416658 1102 32-33 0.841681400878322 1102 34-35 1.1186942036941403 1102 36-37 1.023393866862726 1102 38-39 1.1259663275037468 1102 40-41 1.0935953698883338 1102 42-43 1.2887333255733253 1102 44-45 1.5019611816831855 1102 46-47 1.698280488628221 1102 48-49 1.9232531331799763 1102 50-51 1.9554266954943813 1102 52-53 2.311189616078785 1102 54-55 2.3285341413082605 1102 56-57 2.6549063712747056 1102 58-59 2.812982368580922 1102 60-61 2.9803922720047638 1102 62-63 3.2048570640142806 1102 64-65 3.1111952568477257 1102 66-67 3.401226574501788 1102 68-69 3.6244517998953967 1102 70-71 3.763624326106747 1102 72-73 3.8745481593619857 1102 74-75 3.904907068534058 1102 76-77 4.031299322708012 1102 78-79 4.399970993025999 1102 80-81 4.73565131912709 1102 82-83 4.942817134666715 1102 84-85 5.010932278640933 1102 86-87 5.30514338851539 1102 88-89 5.602711279915859 1102 90-91 5.55139012079276 1102 92-93 5.415719702150529 1102 94-95 5.2116164165546195 1102 96-97 5.08441233033858 1102 98-99 5.382878836758863 1102 100-101 4.077263643384587 1103 1 0.4925975701111014 1103 2 0.36440304255570766 1103 3 0.3474196007844199 1103 4 0.32134768778130507 1103 5 0.33014020041333225 1103 6 0.2855475528046014 1103 7 0.3118771168565573 1103 8 0.43224925562387284 1103 9 0.2861844535325204 1103 10-11 0.2966464905240187 1103 12-13 0.49304249477199136 1103 14-15 0.6300807513057478 1103 16-17 0.6768066843725862 1103 18-19 0.7015517538949467 1103 20-21 0.7017575384881027 1103 22-23 0.7599082213210835 1103 24-25 0.7990537988091262 1103 26-27 0.8991642747234749 1103 28-29 1.001396270049817 1103 30-31 0.8326386636355849 1103 32-33 1.091046461532443 1103 34-35 1.1048525259539872 1103 36-37 1.2044194501853411 1103 38-39 1.4290260028720283 1103 40-41 1.5638432373494382 1103 42-43 1.5051554825453266 1103 44-45 1.5215657153823088 1103 46-47 1.7404384965439803 1103 48-49 1.8894677516091107 1103 50-51 1.9040471535794552 1103 52-53 1.8193732787589951 1103 54-55 2.0597504743415342 1103 56-57 2.2290322803721097 1103 58-59 2.405070899308054 1103 60-61 2.539486818134762 1103 62-63 2.6476869824276967 1103 64-65 2.8219931550288173 1103 66-67 3.0801490886829974 1103 68-69 3.0797865406329663 1103 70-71 3.43813727136801 1103 72-73 3.7705102666104757 1103 74-75 4.030211410769045 1103 76-77 4.275892782922053 1103 78-79 4.506991761168653 1103 80-81 4.572672658804457 1103 82-83 4.867831244429954 1103 84-85 4.864150554892234 1103 86-87 5.082986595110899 1103 88-89 5.060529696193168 1103 90-91 5.282037062367625 1103 92-93 5.253759623850275 1103 94-95 5.310909678160346 1103 96-97 5.303173671730551 1103 98-99 5.0946560239507725 1103 100-101 4.142574879757056 1104 1 0.5278204726475622 1104 2 0.4884806361309231 1104 3 0.4009000488976824 1104 4 0.33502163964976006 1104 5 0.25989507865717343 1104 6 0.34534952539576835 1104 7 0.4458021016976801 1104 8 0.4627395421870659 1104 9 0.6516344030802088 1104 10-11 0.5961224620084522 1104 12-13 0.5753671957422526 1104 14-15 0.8347399820925574 1104 16-17 0.8527327666798996 1104 18-19 0.6602045330111679 1104 20-21 0.7614017130587456 1104 22-23 0.7261535192546731 1104 24-25 0.8425182949168288 1104 26-27 0.9784017311054853 1104 28-29 1.0757009238826782 1104 30-31 1.0856135619941156 1104 32-33 1.106574581537096 1104 34-35 1.1545017772164599 1104 36-37 1.315639899484161 1104 38-39 1.4856488338115668 1104 40-41 1.6413750511192973 1104 42-43 1.7110252708641767 1104 44-45 1.7371938127686875 1104 46-47 1.8783579404061328 1104 48-49 1.883592962765384 1104 50-51 2.196140065318872 1104 52-53 2.3771942487084914 1104 54-55 2.5043357172296155 1104 56-57 2.891693725298616 1104 58-59 3.030123098495846 1104 60-61 3.1366049593436145 1104 62-63 3.2790703097504625 1104 64-65 3.484315491512479 1104 66-67 3.485444611424949 1104 68-69 3.575972398552487 1104 70-71 3.807609663006467 1104 72-73 4.131030394054314 1104 74-75 4.126069982678743 1104 76-77 4.318933062315619 1104 78-79 4.636058454593094 1104 80-81 4.926982200567615 1104 82-83 5.0663744358841605 1104 84-85 5.131995060424767 1104 86-87 5.371842743256252 1104 88-89 5.397029587075753 1104 90-91 5.34284957149524 1104 92-93 5.714445728981179 1104 94-95 5.7455395714306015 1104 96-97 5.55205492847174 1104 98-99 5.500294629062633 1104 100-101 4.524178842822053 1105 1 0.4619272707622599 1105 2 0.3549632805137577 1105 3 0.4208212061519845 1105 4 0.2982264957519689 1105 5 0.37254819385828597 1105 6 0.33560888568240443 1105 7 0.3667398827328938 1105 8 0.4277219144789228 1105 9 0.4638857300863748 1105 10-11 0.4608873477752482 1105 12-13 0.4361887025085238 1105 14-15 0.6198578007455495 1105 16-17 0.7637077960852778 1105 18-19 0.6536488943641316 1105 20-21 0.8231035032093885 1105 22-23 0.8356449155610051 1105 24-25 0.8866280762537855 1105 26-27 0.9026584633006038 1105 28-29 0.997177764796227 1105 30-31 1.0690510908058073 1105 32-33 1.083336180482128 1105 34-35 1.2713647746749714 1105 36-37 1.3918065923820748 1105 38-39 1.5078988290775257 1105 40-41 1.647973994605394 1105 42-43 1.757143372317941 1105 44-45 1.8257840276134658 1105 46-47 1.7616574525049487 1105 48-49 1.8728538283220146 1105 50-51 2.1626272419644295 1105 52-53 2.158087594943254 1105 54-55 2.1879743108747007 1105 56-57 2.4881533141230463 1105 58-59 2.7616436509103828 1105 60-61 2.9680303963266965 1105 62-63 3.056630580307928 1105 64-65 3.2666256354925345 1105 66-67 3.342095970839903 1105 68-69 3.4348184906710415 1105 70-71 3.5490566468632316 1105 72-73 3.939529876724464 1105 74-75 4.141028001819173 1105 76-77 4.421849107736339 1105 78-79 4.357759363125513 1105 80-81 4.407194527763458 1105 82-83 4.70849315695412 1105 84-85 4.570197658156342 1105 86-87 4.810674890906192 1105 88-89 5.1066191099743925 1105 90-91 5.235242634153149 1105 92-93 5.325996821396185 1105 94-95 5.009843904801496 1105 96-97 4.987713250497698 1105 98-99 4.937692594945872 1105 100-101 4.062678552105339 1106 1 0.23515280177357667 1106 2 0.15085117257707736 1106 3 0.11050743553303732 1106 4 0.21068599565165158 1106 5 0.15399206236129004 1106 6 0.22906441737453065 1106 7 0.12499923393350798 1106 8 0.20115948711517717 1106 9 0.1556338918174518 1106 10-11 0.181160956450789 1106 12-13 0.20099705077024765 1106 14-15 0.3911863539948399 1106 16-17 0.45557592478944997 1106 18-19 0.32919431670935495 1106 20-21 0.41094437061859423 1106 22-23 0.35989503950188606 1106 24-25 0.2850054029757345 1106 26-27 0.45505838760144 1106 28-29 0.4617402707383391 1106 30-31 0.49349349181280644 1106 32-33 0.5066327469373846 1106 34-35 0.519193897317507 1106 36-37 0.6145767137676614 1106 38-39 0.6056134282483754 1106 40-41 0.877228030969178 1106 42-43 0.659916438892715 1106 44-45 0.9417689969514029 1106 46-47 1.181978088233933 1106 48-49 1.4335502186057667 1106 50-51 1.6929192025876745 1106 52-53 1.484075950349009 1106 54-55 1.5097464270111303 1106 56-57 1.312591096401583 1106 58-59 1.8506933355322097 1106 60-61 2.1651580270899444 1106 62-63 1.867148594698559 1106 64-65 2.133733420719256 1106 66-67 2.2035437142761367 1106 68-69 2.154339006672984 1106 70-71 2.5644034218960634 1106 72-73 2.841247683812174 1106 74-75 3.0764416824329004 1106 76-77 2.8186434702483396 1106 78-79 3.3729873708549825 1106 80-81 3.4257841600376935 1106 82-83 3.2367257345255176 1106 84-85 3.578587398460833 1106 86-87 3.4387883712650513 1106 88-89 3.66349890096793 1106 90-91 3.6840876671155733 1106 92-93 3.8356315189639467 1106 94-95 3.573455042655244 1106 96-97 3.497625821566885 1106 98-99 3.2667747486289613 1106 100-101 2.6210646784047693 1107 1 0.4799012515827883 1107 2 0.32966598824928184 1107 3 0.27509707854976995 1107 4 0.31953304115873493 1107 5 0.31102759985874684 1107 6 0.16518623098124152 1107 7 0.28874543670092834 1107 8 0.2641637731562554 1107 9 0.3652496589414369 1107 10-11 0.29535542532254055 1107 12-13 0.36110814485754616 1107 14-15 0.45864908529743786 1107 16-17 0.5389618271837051 1107 18-19 0.4640302266120315 1107 20-21 0.5372459688906517 1107 22-23 0.5339017279312728 1107 24-25 0.6041959074650975 1107 26-27 0.5822212452830087 1107 28-29 0.66264399153129 1107 30-31 0.6644045199844442 1107 32-33 0.6574818375357836 1107 34-35 0.7945268795473908 1107 36-37 0.8885172123934524 1107 38-39 0.8548239785314564 1107 40-41 0.7620623417164438 1107 42-43 0.8574016223371572 1107 44-45 0.891466269218725 1107 46-47 1.107837131670955 1107 48-49 1.1530667375764736 1107 50-51 1.1559340887445586 1107 52-53 1.2823613816168518 1107 54-55 1.4448280281037924 1107 56-57 1.6110046124021267 1107 58-59 1.7514150267502195 1107 60-61 1.8092273079847914 1107 62-63 2.0128694223726633 1107 64-65 2.0220700340980073 1107 66-67 2.2472436055031118 1107 68-69 2.42966635378383 1107 70-71 2.6922915346276888 1107 72-73 2.78273854745661 1107 74-75 3.0785746511384957 1107 76-77 3.1311712841298025 1107 78-79 3.120798431710309 1107 80-81 3.2714971899254515 1107 82-83 3.6911240667739627 1107 84-85 3.7995827554273482 1107 86-87 3.910054594696607 1107 88-89 3.9287544627542665 1107 90-91 3.8029845666730786 1107 92-93 3.662140063175112 1107 94-95 3.7258385609372446 1107 96-97 3.7027050132654082 1107 98-99 3.6958418501034416 1107 100-101 3.150724289800868 1108 1 0.3517727111843101 1108 2 0.28830412357453383 1108 3 0.23883704228973102 1108 4 0.20517346299548223 1108 5 0.23497749681477842 1108 6 0.08030819150197033 1108 7 0.15758834112665454 1108 8 0.18744630748172852 1108 9 0.2323758524480013 1108 10-11 0.33614748942748207 1108 12-13 0.37430667093950376 1108 14-15 0.4384349890097212 1108 16-17 0.42681344386966913 1108 18-19 0.48533196509168164 1108 20-21 0.48554125460925235 1108 22-23 0.4020534353467866 1108 24-25 0.4138470833739376 1108 26-27 0.5761444318442344 1108 28-29 0.6013076455323656 1108 30-31 0.6014062821395854 1108 32-33 0.695205304707109 1108 34-35 0.6903301219763947 1108 36-37 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2311 42-43 0.811563452508274 2311 44-45 0.8564280729178364 2311 46-47 0.8701009603410697 2311 48-49 1.0039896741125887 2311 50-51 0.9296169898345497 2311 52-53 0.9981064298554898 2311 54-55 1.4011499079016758 2311 56-57 1.5486935240649942 2311 58-59 1.6895360307202019 2311 60-61 1.7680080085757197 2311 62-63 1.7490127424617548 2311 64-65 2.0687730750557414 2311 66-67 1.9240886753532287 2311 68-69 2.1156571715133694 2311 70-71 2.702478038683754 2311 72-73 3.0044985622633575 2311 74-75 3.2597337789134215 2311 76-77 3.148899837893371 2311 78-79 3.5342892130545778 2311 80-81 3.445003464531851 2311 82-83 3.635657529281886 2311 84-85 3.890529467338073 2311 86-87 3.819336354854208 2311 88-89 3.965351184564536 2311 90-91 4.353184713147844 2311 92-93 4.631493500952036 2311 94-95 4.494783275365009 2311 96-97 4.320134850783873 2311 98-99 4.371241532054945 2311 100-101 4.295078819136052 2312 1 -0.2471192224404497 2312 2 -0.19324994223525493 2312 3 -0.21614164455918328 2312 4 -0.03521006498346679 2312 5 -0.21588100995975879 2312 6 -0.14997333782535804 2312 7 -0.12097291243459551 2312 8 -0.017404337349830712 2312 9 -0.05078539893844436 2312 10-11 -0.21178589610131837 2312 12-13 -0.19453059336532164 2312 14-15 -0.06367226724830033 2312 16-17 -0.15075310535717534 2312 18-19 -0.18203770933906327 2312 20-21 -0.1913252772191143 2312 22-23 -0.1278310549861743 2312 24-25 -0.07816469866646969 2312 26-27 -0.25157951935300105 2312 28-29 -0.1617518246835914 2312 30-31 0.03397761595137183 2312 32-33 0.05616078088204546 2312 34-35 -0.01724882367506808 2312 36-37 0.07586314632651181 2312 38-39 0.24414613573312494 2312 40-41 0.24709797647928866 2312 42-43 0.40117048811288925 2312 44-45 0.5438819133905071 2312 46-47 0.5292837934131782 2312 48-49 0.689034645002728 2312 50-51 0.7770241633774617 2312 52-53 0.9075287521800703 2312 54-55 1.0450976304164286 2312 56-57 1.1461940737509693 2312 58-59 1.1279958669983436 2312 60-61 1.1948285967115169 2312 62-63 1.2731660381345513 2312 64-65 1.5471813395138945 2312 66-67 1.7650671069910864 2312 68-69 2.0286432912377954 2312 70-71 2.3175435217872504 2312 72-73 2.714575621659513 2312 74-75 2.366656509978853 2312 76-77 2.460297647042399 2312 78-79 2.9774007081628433 2312 80-81 3.3818054338170924 2312 82-83 3.52115101103983 2312 84-85 4.054784680428309 2312 86-87 4.16734665606333 2312 88-89 4.02398059661666 2312 90-91 4.175352139038896 2312 92-93 4.3793752132285455 2312 94-95 4.333665383227494 2312 96-97 4.206521807158538 2312 98-99 4.222692186228237 2312 100-101 3.9128156821110807 2313 1 -0.4506519549921215 2313 2 -0.3105353799194077 2313 3 -0.3333142562367435 2313 4 -0.11200935940595969 2313 5 -0.15579880623233322 2313 6 -0.11382101419281554 2313 7 -0.047924017277097164 2313 8 -0.021443867275863226 2313 9 -0.02954113669042613 2313 10-11 -0.07502148577533774 2313 12-13 -0.15015778483665798 2313 14-15 -0.13888268887212973 2313 16-17 -0.044612544340516536 2313 18-19 -0.17636321169517544 2313 20-21 -0.1319971908558486 2313 22-23 -0.1377446028997653 2313 24-25 -0.05130145455315471 2313 26-27 -0.07303763510934402 2313 28-29 0.05170847491768171 2313 30-31 0.09463337057118082 2313 32-33 0.11836862208762966 2313 34-35 0.20851511829961566 2313 36-37 0.4252330254165102 2313 38-39 0.6208805832401012 2313 40-41 0.7422300838447882 2313 42-43 0.6188786980778218 2313 44-45 0.8169337469589095 2313 46-47 0.8628689104292206 2313 48-49 1.2605048732925823 2313 50-51 1.225211231139454 2313 52-53 1.3301473832305106 2313 54-55 1.6080688976924975 2313 56-57 1.8826501303766108 2313 58-59 1.7667819488898395 2313 60-61 2.0166835100934435 2313 62-63 2.025078921204642 2313 64-65 2.430158900821258 2313 66-67 2.466172622410788 2313 68-69 2.4820809179707837 2313 70-71 2.9298080900247783 2313 72-73 3.366133697220185 2313 74-75 3.423829574754887 2313 76-77 3.528399469485219 2313 78-79 3.8468538407331785 2313 80-81 3.9706882141011164 2313 82-83 4.078089675861801 2313 84-85 4.179960424317311 2313 86-87 4.385907117344495 2313 88-89 4.570650636848217 2313 90-91 4.651537328012363 2313 92-93 4.624360063589403 2313 94-95 4.537461675536122 2313 96-97 4.485262606119193 2313 98-99 4.137980491442548 2313 100-101 3.6759333315976903 2314 1 -0.28150603844958155 2314 2 -0.3011412527867279 2314 3 -0.1611815960466636 2314 4 -0.024364306261915658 2314 5 -0.2011542495973444 2314 6 -0.011468308218454126 2314 7 -0.23382337972431344 2314 8 -0.13763423675769104 2314 9 -0.14945576349576584 2314 10-11 -0.17804294111808616 2314 12-13 -0.06852422609699715 2314 14-15 -0.07348223015048205 2314 16-17 -0.05149952042459205 2314 18-19 -0.005386143383439901 2314 20-21 -0.0810841414553991 2314 22-23 -0.10686767123787178 2314 24-25 5.446364266390447E-4 2314 26-27 0.027458434560102774 2314 28-29 -0.0916610420624906 2314 30-31 -0.020685471727880156 2314 32-33 0.036133760121536795 2314 34-35 0.19093320528975966 2314 36-37 0.25811862182055734 2314 38-39 0.30645128305423697 2314 40-41 0.26083665094600406 2314 42-43 0.5224152266446751 2314 44-45 0.5544543432970528 2314 46-47 0.5555434245753403 2314 48-49 0.7111292444177195 2314 50-51 0.8824497180610535 2314 52-53 1.1112750959055404 2314 54-55 1.2229067193798357 2314 56-57 1.3597673240898374 2314 58-59 1.4860334285307708 2314 60-61 1.7943425797321382 2314 62-63 1.9372453703739758 2314 64-65 2.1436405497585973 2314 66-67 2.3262151771915534 2314 68-69 2.594912821094397 2314 70-71 2.8999390058423273 2314 72-73 3.0893874572876783 2314 74-75 3.349385071725301 2314 76-77 3.3480277248051173 2314 78-79 3.586194494279699 2314 80-81 3.8235730196057744 2314 82-83 4.0869902598091805 2314 84-85 4.427854435012026 2314 86-87 4.457739125921361 2314 88-89 4.5290191212653 2314 90-91 4.5725064873302586 2314 92-93 4.731231441380071 2314 94-95 4.729706797224782 2314 96-97 4.994994732891577 2314 98-99 4.6921676446002145 2314 100-101 4.100140894353359 2315 1 -0.4483790093157154 2315 2 -0.46837614832113417 2315 3 -0.32393633533240873 2315 4 -0.3026663870075268 2315 5 -0.26597774172066835 2315 6 -0.28212414141615483 2315 7 -0.20862185835576952 2315 8 -0.3774900665288854 2315 9 -0.4441202657529786 2315 10-11 -0.4801588084095201 2315 12-13 -0.3356520433271797 2315 14-15 -0.4679867574211869 2315 16-17 -0.42686936888738103 2315 18-19 -0.39921800474701996 2315 20-21 -0.4796681379603598 2315 22-23 -0.4327733395349824 2315 24-25 -0.23762363927001218 2315 26-27 -0.2750968820918587 2315 28-29 -0.23546956035574595 2315 30-31 -0.18781842871666043 2315 32-33 -0.07760695618948432 2315 34-35 -0.05158116461866058 2315 36-37 0.06790857701899711 2315 38-39 -0.08520517548075901 2315 40-41 0.07026339653651803 2315 42-43 0.13605593181954845 2315 44-45 0.1528702268643869 2315 46-47 0.35623386548096647 2315 48-49 0.682788187169372 2315 50-51 0.6885994392291508 2315 52-53 0.8511842506722473 2315 54-55 0.9821756315499286 2315 56-57 1.4475233483152365 2315 58-59 1.5940011251479511 2315 60-61 1.7309355194170735 2315 62-63 1.9049171453896072 2315 64-65 2.1976724762257867 2315 66-67 2.173498600613435 2315 68-69 2.655697747895669 2315 70-71 2.8285745284272856 2315 72-73 3.172749630377613 2315 74-75 3.6497825463905755 2315 76-77 3.883375343811945 2315 78-79 4.019609778208533 2315 80-81 4.175300835122705 2315 82-83 4.464541721642188 2315 84-85 4.615433351722238 2315 86-87 4.641534537830182 2315 88-89 4.9817579701306975 2315 90-91 4.912168719527418 2315 92-93 5.238414254027305 2315 94-95 5.419600532715673 2315 96-97 5.471724261661375 2315 98-99 5.449967656592985 2315 100-101 5.040620990638171 2316 1 -0.7189075622673258 2316 2 -0.7821669159496203 2316 3 -0.5943969378264633 2316 4 -0.7406007354346116 2316 5 -0.5455413770066357 2316 6 -0.41563938158186176 2316 7 -0.4109844597787031 2316 8 -0.44800634182772825 2316 9 -0.7296817460992102 2316 10-11 -0.5683313009635427 2316 12-13 -0.5567329848114682 2316 14-15 -0.668590796768946 2316 16-17 -0.5882632423386482 2316 18-19 -0.802252172607929 2316 20-21 -0.7637879466830029 2316 22-23 -0.6521019636123029 2316 24-25 -0.76401688825446 2316 26-27 -0.6672341039130529 2316 28-29 -0.7023973741079956 2316 30-31 -0.754690781347783 2316 32-33 -0.4861202382812593 2316 34-35 -0.3276817609315614 2316 36-37 -0.11424977083230914 2316 38-39 -0.33774051077710254 2316 40-41 -0.32353342524488227 2316 42-43 -0.10931137109766809 2316 44-45 0.20512613971845184 2316 46-47 0.3171695496319984 2316 48-49 0.35934210405813616 2316 50-51 0.29430463161785525 2316 52-53 0.41168249052253714 2316 54-55 0.7776589371174154 2316 56-57 0.8250438128338757 2316 58-59 1.0645623832263347 2316 60-61 0.9353097507407568 2316 62-63 1.1699847889058432 2316 64-65 1.709811687873465 2316 66-67 1.7932701727668707 2316 68-69 2.0587016449920057 2316 70-71 2.348889492557486 2316 72-73 2.822004927232207 2316 74-75 3.08317130556442 2316 76-77 3.1739730961547536 2316 78-79 3.3016657770061677 2316 80-81 3.5078384142659402 2316 82-83 3.7555917361425237 2316 84-85 3.9180219254611863 2316 86-87 4.220100719670683 2316 88-89 4.4133411734549775 2316 90-91 4.4402462296194365 2316 92-93 4.550964449517171 2316 94-95 4.6592372412039715 2316 96-97 4.7585941587619445 2316 98-99 4.5594940567216895 2316 100-101 4.479968115868111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6125.0 3 1984.0 4 2219.0 5 2368.0 6 2946.0 7 3586.0 8 4516.0 9 5845.0 10 7457.0 11 9368.0 12 11751.0 13 14508.0 14 17106.0 15 20699.0 16 24656.0 17 30655.0 18 30423.0 19 28826.0 20 29772.0 21 32457.0 22 35411.0 23 40112.0 24 43830.0 25 48201.0 26 52034.0 27 56627.0 28 61709.0 29 66972.0 30 73330.0 31 82074.0 32 92140.0 33 106379.0 34 127967.0 35 158144.0 36 190497.0 37 206806.0 38 117246.0 39 8371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73274532615459 18.58104488514921 14.653174968209735 23.03303482048646 2 15.290510808795421 19.807003704748126 40.62190810434546 24.28057738211099 3 19.216212455737487 23.9511551126638 30.046681241971267 26.785951189627443 4 12.582123268353737 16.69390852022 37.29570632077512 33.42826189065114 5 13.386803596567404 37.56050464134111 34.43389662295913 14.618795139132356 6 32.04165738531895 36.22821490153861 17.237633604213848 14.492494108928595 7 28.281526520637705 33.29862530317851 20.358389619297306 18.06145855688648 8 26.51049305495643 34.571272970408074 19.64972823742559 19.268505737209903 9 26.40182821251568 16.7635397136354 20.380654724832556 36.453977349016355 10-11 24.927307496635994 26.473524561236545 27.145625225626475 21.45354271650099 12-13 26.18994018788848 23.903141315381163 27.522468297693653 22.3844501990367 14-15 22.581420891131657 25.506577241133517 26.302979587017084 25.609022280717742 16-17 21.710717346719512 28.50500889838745 27.35969031832259 22.424583436570444 18-19 21.77360805009065 26.837886109819305 29.876458337545746 21.5120475025443 20-21 22.486916288513026 25.912004119919867 30.17730754608621 21.423772045480895 22-23 21.993232668992537 26.090457718200057 30.01646406280877 21.899845549998638 24-25 21.911482566566043 26.212156552385242 30.171725492681073 21.70463538836764 26-27 21.44142327160815 26.459258116816148 30.044562172008852 22.05475643956685 28-29 21.475013642255835 27.108321668216025 29.738257414808693 21.678407274719444 30-31 21.717189420285713 26.43537270428537 29.70401412009088 22.14342375533804 32-33 21.331453527655412 26.579675773963885 29.71961452659472 22.369256171785985 34-35 22.17499645447246 26.773154721429382 29.449178704833617 21.602670119264538 36-37 22.2680277838742 26.22433413123087 29.598987736032704 21.908650348862228 38-39 21.762312405245645 26.69201896057572 29.424421877122874 22.12124675705576 40-41 22.2668644522623 26.43127916477188 29.483999017721185 21.817857365244635 42-43 21.873931030095452 26.573961732865413 29.14496155145221 22.407145685586922 44-45 21.90615843681854 26.718806858461345 29.411246216174693 21.963788488545422 46-47 22.373575836975874 26.995270424179196 28.688644570153127 21.9425091686918 48-49 22.090652370575555 27.04591598530568 28.58273859135916 22.280693052759606 50-51 22.155610340167154 27.35917207070198 28.559345014208 21.92587257492287 52-53 22.509639938265583 27.091770918517792 28.4256753077961 21.972913835420528 54-55 21.905366694087547 27.185431641684193 28.659771195568386 22.249430468659874 56-57 22.24671188734095 27.29561409774512 28.253873699276678 22.203800315637253 58-59 22.26579061494055 27.031740927746217 28.2174403006255 22.48502815668773 60-61 21.860043002567917 27.191240133789364 28.724362272780326 22.2243545908624 62-63 21.974223222280504 27.15243587207137 28.82000729917957 22.053333606468563 64-65 21.668595259408526 27.283266472756168 28.547054024047164 22.50108424378814 66-67 21.660908943154734 27.258726138375657 28.40301135633134 22.677353562138265 68-69 22.303489112393052 26.826726052143425 28.53230648641859 22.337478349044932 70-71 22.128482746796667 26.858974566338112 28.169745728078606 22.84279695878662 72-73 22.064365834335437 27.04727416037034 28.350829352554292 22.53753065273994 74-75 22.0458390253843 27.50074666051368 28.036751870425018 22.416662443676998 76-77 22.098539845944057 26.915584599365328 28.26034629567884 22.725529259011775 78-79 22.19008381412913 27.210360121779143 27.98866305749738 22.61089300659435 80-81 22.538827771786316 27.19576140546844 27.67965467616591 22.585756146579335 82-83 22.452468377393565 27.20080563991066 27.900565144752637 22.44616083794314 84-85 22.33499035463203 27.305596038458564 27.794805005079414 22.564608601829992 86-87 22.4594558973473 27.33606830216761 27.484577055963545 22.71989874452155 88-89 22.350549137412543 27.292664535662865 27.369524093489904 22.987262233434688 90-91 22.43508092593536 27.24968816577315 27.409790475974816 22.905440432316674 92-93 22.647501298606077 26.909786570351024 27.667924524233058 22.774787606809845 94-95 22.345155408035733 26.654583628534734 27.847925178981015 23.15233578444852 96-97 22.517798129622733 26.293975040968924 27.87374859112462 23.314478238283723 98-99 22.356361252366842 26.475286716785497 27.358485479773474 23.80986655107419 100-101 23.114694118078773 25.428559556368143 27.694855234670285 23.761891090882795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 731.0 1 521.0 2 402.0 3 493.0 4 398.5 5 381.0 6 741.0 7 1580.0 8 2559.0 9 2974.5 10 2505.5 11 1885.5 12 1788.5 13 1929.5 14 2081.5 15 2192.5 16 2307.0 17 2572.5 18 2885.5 19 3255.0 20 3762.0 21 4382.5 22 5229.5 23 6408.5 24 8028.5 25 10030.0 26 12557.0 27 15620.0 28 18137.5 29 20636.0 30 23734.5 31 28018.5 32 33528.0 33 36098.0 34 37114.0 35 39699.5 36 42990.5 37 46432.5 38 48946.0 39 50191.5 40 51890.0 41 54705.5 42 56927.0 43 58710.5 44 60638.0 45 63524.0 46 65690.0 47 67842.5 48 70554.0 49 69979.0 50 66883.5 51 63846.0 52 61748.5 53 60906.0 54 60488.0 55 57997.5 56 53493.5 57 48399.0 58 44121.5 59 38887.5 60 32013.0 61 26981.5 62 22976.5 63 17971.0 64 13125.0 65 9850.0 66 7499.0 67 5791.0 68 4434.5 69 3269.0 70 2465.0 71 1781.5 72 1270.5 73 938.5 74 690.0 75 461.5 76 313.0 77 229.5 78 147.0 79 101.5 80 71.0 81 44.5 82 30.5 83 18.0 84 12.0 85 9.5 86 6.5 87 6.0 88 4.0 89 1.5 90 2.0 91 2.0 92 1.5 93 1.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38094632306210335 2 0.025335329254165644 3 0.04522625796647867 4 0.0398896673363459 5 0.06075088525413761 6 0.05606115409432397 7 0.03228906856009621 8 0.030025060413979277 9 0.03385231228003409 10-11 0.030105917847769174 12-13 0.030159822803629097 14-15 0.025011899519006082 16-17 0.03059106245050851 18-19 0.025847426334834944 20-21 0.038380328572267954 22-23 0.025416186687955533 24-25 0.045792260003007894 26-27 0.039970524770135796 28-29 0.03153439917805723 30-31 0.039781857424626046 32-33 0.03611632042615102 34-35 0.036008510514431165 36-37 0.03557727086755175 38-39 0.017303490831036532 40-41 0.013287571619471979 42-43 0.012694617105012783 44-45 0.009810701966506696 46-47 0.016144534280048105 48-49 0.013530143920841651 50-51 0.009487272231347134 52-53 0.003988966733634591 54-55 0.0044741113363739315 56-57 0.007519741342459802 58-59 0.0027761052267862353 60-61 0.006010402578381848 62-63 0.006145164968031666 64-65 0.007411931430739948 66-67 0.003908109299844699 68-69 0.006360784791471373 70-71 0.017842540389635803 72-73 0.014365670736670517 74-75 0.010295846569246037 76-77 0.007627551254179656 78-79 0.006441642225261264 80-81 0.00875955532723812 82-83 0.010457561436825817 84-85 0.004393253902584042 86-87 4.0428716894945173E-4 88-89 0.023475608276998162 90-91 0.01970226136680328 92-93 0.013125856751892198 94-95 0.02431113509282703 96-97 0.019271021719923863 98-99 0.01751911065447624 100-101 0.1104512545569902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1855117.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.195246352511866 #Duplication Level Percentage of deduplicated Percentage of total 1 79.09528408861611 48.40255395124775 2 12.508169213947266 15.308809929328152 3 3.876834628019337 7.117315503887761 4 1.6786318608143547 4.1089716103083935 5 0.8381125318038345 2.5644251427431546 6 0.5004932084173318 1.8376683112114611 7 0.32265485692850504 1.3821460419602034 8 0.20263505930382839 0.9920241899002903 9 0.1545582513838689 0.8512407242324382 >10 0.7515044859069591 8.096318707963624 >50 0.04096812338417347 1.7071875623440134 >100 0.023964854375539697 2.8461715919384845 >500 0.0035364783422980454 1.476766382970521 >1k 0.002475534839608631 2.431105354548356 >5k 1.7682391711490222E-4 0.8772949954155337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8923 0.4809939211381277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7292 0.39307493813058686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4429 0.2387450495036162 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2482 0.13379210044433854 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2423 0.13061170804860286 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2237 0.12058538625865647 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2156 0.1162190848340024 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2150 0.11589565509884284 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1944 0.10479123419169789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6952477929963446E-5 2.6952477929963446E-5 12-13 0.0 0.0 0.0 5.390495585992689E-5 1.0780991171985379E-4 14-15 0.0 0.0 0.0 5.390495585992689E-5 1.0780991171985379E-4 16-17 0.0 0.0 0.0 5.390495585992689E-5 2.1561982343970757E-4 18-19 0.0 0.0 0.0 5.390495585992689E-5 2.6952477929963447E-4 20-21 0.0 0.0 0.0 8.085743378989034E-5 4.3123964687941514E-4 22-23 0.0 0.0 0.0 2.6952477929963447E-4 4.3123964687941514E-4 24-25 0.0 0.0 0.0 7.8162185996894E-4 4.8514460273934207E-4 26-27 0.0 0.0 0.0 0.0017249585875176608 5.120970806693055E-4 28-29 0.0 0.0 0.0 0.00477058859360353 5.390495585992689E-4 30-31 0.0 0.0 0.0 0.015389864898009128 5.390495585992689E-4 32-33 0.0 0.0 0.0 0.03746394432264919 5.390495585992689E-4 34-35 0.0 0.0 0.0 0.07013034757376489 5.929545144591959E-4 36-37 0.0 0.0 0.0 0.1256524521094896 5.929545144591959E-4 38-39 0.0 0.0 0.0 0.2083157019206875 5.929545144591959E-4 40-41 0.0 0.0 0.0 0.29636944731787807 6.468594703191227E-4 42-43 2.6952477929963446E-5 0.0 0.0 0.3807576557165936 6.468594703191227E-4 44-45 5.390495585992689E-5 0.0 0.0 0.4856027948641515 7.277169041090131E-4 46-47 8.085743378989034E-5 0.0 0.0 0.6051100820056093 7.546693820389765E-4 48-49 1.0780991171985379E-4 0.0 0.0 0.7282290011896824 7.546693820389765E-4 50-51 1.0780991171985379E-4 2.6952477929963446E-5 0.0 0.8587328993265654 7.546693820389765E-4 52-53 1.0780991171985379E-4 5.390495585992689E-5 0.0 0.9884821280814093 8.355268158288669E-4 54-55 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.1232175652532965 9.163842496187572E-4 56-57 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.274178394139022 9.163842496187572E-4 58-59 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.4318773425072382 9.163842496187572E-4 60-61 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.6018666208115175 9.163842496187572E-4 62-63 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.7664654035298044 9.163842496187572E-4 64-65 1.0780991171985379E-4 5.390495585992689E-5 0.0 1.9353765827168852 9.163842496187572E-4 66-67 1.0780991171985379E-4 5.390495585992689E-5 0.0 2.1246638352190184 9.163842496187572E-4 68-69 1.0780991171985379E-4 5.390495585992689E-5 0.0 2.3362407869692317 9.163842496187572E-4 70-71 1.0780991171985379E-4 5.390495585992689E-5 0.0 2.5502973666890014 9.702892054786841E-4 72-73 1.0780991171985379E-4 5.390495585992689E-5 0.0 2.765971095084569 0.0010511466392685745 74-75 1.0780991171985379E-4 5.390495585992689E-5 0.0 2.97913824303265 0.0010780991171985379 76-77 1.0780991171985379E-4 5.390495585992689E-5 0.0 3.2092854520766076 0.0011050515951285013 78-79 1.0780991171985379E-4 5.390495585992689E-5 0.0 3.464794942852661 0.0011320040730584647 80-81 1.0780991171985379E-4 5.390495585992689E-5 0.0 3.7355595361370737 0.0011320040730584647 82-83 1.0780991171985379E-4 5.390495585992689E-5 0.0 4.005758127384958 0.0011320040730584647 84-85 1.0780991171985379E-4 5.390495585992689E-5 0.0 4.276064528544561 0.0011589565509884281 86-87 1.0780991171985379E-4 5.390495585992689E-5 0.0 4.555615629634142 0.0012128615068483551 88-89 1.0780991171985379E-4 5.390495585992689E-5 0.0 4.847133630924626 0.0012398139847783186 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6500 0.0 33.975353 1 GTATCAA 9735 0.0 32.04337 1 ATCAACG 11725 0.0 26.062124 3 TATCAAC 11800 0.0 26.017302 2 TCAACGC 11725 0.0 25.98106 4 CAACGCA 11780 0.0 25.819416 5 GTGTAAG 705 0.0 25.03277 1 AACGCAG 12170 0.0 25.027006 6 GTACATG 6825 0.0 22.713133 1 ACGCAGA 13430 0.0 22.602728 7 GAGTACT 7735 0.0 22.537546 12-13 CGCAGAG 13560 0.0 22.281536 8 TACATGG 6860 0.0 21.960733 2 GTACTTT 8165 0.0 21.120493 14-15 GCAGAGT 14160 0.0 20.765945 9 AGAGTAC 10960 0.0 20.696035 10-11 ACATGGG 7035 0.0 20.401146 3 AGTACTT 8055 0.0 19.548744 12-13 GTGTAGC 1200 0.0 19.476511 1 CGCCGTA 200 1.8189894E-12 19.014673 94-95 >>END_MODULE