FastQCFastQC Report
Thu 26 May 2016
SRR938075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855117
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88770.478514293168571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75080.40471840859633107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.254377486702995No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24590.13255228645956024No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23330.12576026202120943No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22710.12241815475789398No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22630.12198691511101456No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22630.12198691511101456No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22350.12047757634693661No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19870.10710914729367474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.034.1192171
GTATCAA98750.032.703621
TAAGGTG11450.028.2405285
ATCAACG120000.026.5117073
TCAACGC121100.026.3480074
TATCAAC121200.026.3319532
GTAAGGT12350.026.1825164
CAACGCA122050.026.1429215
GTGTAGC12950.025.709371
AACGCAG125600.025.4488286
GGTAAGG12900.024.3302783
AGGTAAG12050.024.07722
AAGGTAA12200.023.7811681
TATACCG1000.00164351323.7760335
ACGCAGA138650.022.7794977
TACATGG67500.022.7594592
GTACATG68900.022.711191
CGCAGAG140100.022.4759338
GAGTACT83800.022.38697612-13
ACATGGG66900.021.8936143
Produced by FastQC (version 0.11.5)