##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855117 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45601975508822 33.0 31.0 34.0 31.0 34.0 2 32.68331647006631 34.0 31.0 34.0 31.0 34.0 3 32.74917808418552 34.0 31.0 34.0 31.0 34.0 4 36.21972899822491 37.0 35.0 37.0 35.0 37.0 5 36.14856475359775 37.0 35.0 37.0 35.0 37.0 6 36.202371063388455 37.0 37.0 37.0 35.0 37.0 7 36.17033588717046 37.0 37.0 37.0 35.0 37.0 8 36.16367916417131 37.0 37.0 37.0 35.0 37.0 9 37.8487054994375 39.0 38.0 39.0 35.0 39.0 10-11 37.819205742818376 39.0 38.0 39.0 35.0 39.0 12-13 37.74308008605387 39.0 38.0 39.0 35.0 39.0 14-15 38.97838087840282 40.0 38.0 41.0 35.5 41.0 16-17 38.891839436542284 40.0 38.0 41.0 35.0 41.0 18-19 39.1478766568362 40.0 38.5 41.0 36.0 41.0 20-21 39.116584290909955 40.0 39.0 41.0 36.0 41.0 22-23 39.017328556635505 40.0 38.5 41.0 35.0 41.0 24-25 38.909582791813136 40.0 38.0 41.0 35.0 41.0 26-27 38.75463757811502 40.0 38.0 41.0 35.0 41.0 28-29 38.63050659338468 40.0 38.0 41.0 34.5 41.0 30-31 38.378970975954616 40.0 38.0 41.0 34.0 41.0 32-33 38.140510814142715 40.0 38.0 41.0 34.0 41.0 34-35 38.03556406415336 40.0 37.5 41.0 33.0 41.0 36-37 37.97358360685606 40.0 37.5 41.0 33.0 41.0 38-39 37.783202622799536 40.0 37.0 41.0 33.0 41.0 40-41 37.56544358118653 40.0 37.0 41.0 33.0 41.0 42-43 37.30758464290932 40.0 37.0 41.0 32.0 41.0 44-45 37.02255545068047 40.0 36.0 41.0 31.0 41.0 46-47 36.63610974402154 39.5 36.0 41.0 30.5 41.0 48-49 36.20051511575819 39.0 35.0 41.0 30.0 41.0 50-51 35.8112553008786 39.0 35.0 40.0 28.5 41.0 52-53 35.277518884253666 38.0 35.0 40.0 27.0 41.0 54-55 34.90475506396632 38.0 34.0 40.0 26.0 41.0 56-57 34.52578597468516 38.0 34.0 40.0 25.5 41.0 58-59 34.0246148895191 37.5 33.5 40.0 23.5 41.0 60-61 33.55167922023247 37.0 33.0 40.0 22.5 41.0 62-63 33.00809059482502 36.0 32.0 39.0 21.0 40.5 64-65 32.448273073881595 36.0 31.5 39.0 18.5 40.0 66-67 31.888658505096984 35.0 31.0 38.5 15.0 40.0 68-69 31.284522216118983 35.0 30.0 38.0 9.5 40.0 70-71 30.660381258971807 34.5 29.5 37.5 7.5 39.5 72-73 29.99491622361285 34.0 29.0 37.0 4.0 39.0 74-75 29.352906582172444 34.0 28.0 36.0 2.0 39.0 76-77 28.063192779754594 32.5 26.5 35.0 2.0 37.0 78-79 28.71407544645432 34.0 28.0 35.0 2.0 37.0 80-81 28.435421862879807 34.0 27.5 35.0 2.0 37.0 82-83 27.883741834073 33.5 26.5 35.0 2.0 36.5 84-85 27.224509289710568 33.0 26.0 35.0 2.0 36.0 86-87 26.521850373857824 32.5 24.5 35.0 2.0 35.5 88-89 25.720413052114772 32.0 22.0 34.5 2.0 35.0 90-91 25.061860518770516 31.5 19.0 34.0 2.0 35.0 92-93 24.140047770571883 31.0 11.5 34.0 2.0 35.0 94-95 23.236288061615518 31.0 2.0 34.0 2.0 35.0 96-97 22.1814494719201 30.0 2.0 34.0 2.0 35.0 98-99 20.77814310364252 29.0 2.0 34.0 2.0 35.0 100-101 16.938193116660564 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14523181302010357 1101 2 0.15449617912758384 1101 3 0.20508230540841765 1101 4 0.04196530966053302 1101 5 -0.03810113370848711 1101 6 0.04902541834743346 1101 7 0.09094563787063947 1101 8 0.020294112945698828 1101 9 0.012075762414205826 1101 10-11 0.1572138528189555 1101 12-13 0.21236356096235198 1101 14-15 0.3573034021075969 1101 16-17 0.34322548126750974 1101 18-19 0.3261794603536643 1101 20-21 0.21291029682853235 1101 22-23 0.32311761701158304 1101 24-25 0.3439767240013367 1101 26-27 0.3330076239123727 1101 28-29 0.38651463223700944 1101 30-31 0.472848576147328 1101 32-33 0.5029715885300376 1101 34-35 0.3599390729630372 1101 36-37 0.4559245224191599 1101 38-39 0.5433217135301902 1101 40-41 0.569291358065307 1101 42-43 0.7559131615164034 1101 44-45 0.7686802854434234 1101 46-47 0.8839559001258266 1101 48-49 1.0180854119537628 1101 50-51 0.837815073927672 1101 52-53 0.9684397246083662 1101 54-55 1.0656987309300092 1101 56-57 1.1550060182250874 1101 58-59 1.2668331253616714 1101 60-61 1.082173106491986 1101 62-63 1.1769840634416084 1101 64-65 1.3504753871102722 1101 66-67 1.510503189968567 1101 68-69 1.6092405022739875 1101 70-71 1.7828088674430056 1101 72-73 2.005695221100652 1101 74-75 1.8843249361849743 1101 76-77 1.7446977809516468 1101 78-79 1.7979634972319616 1101 80-81 1.9774157632380103 1101 82-83 2.1606932571034783 1101 84-85 2.3906762293673545 1101 86-87 2.447414858607331 1101 88-89 2.697166198694532 1101 90-91 3.170842387956217 1101 92-93 3.3579151334119537 1101 94-95 3.6437794638670233 1101 96-97 3.856428343125156 1101 98-99 4.021758067126672 1101 100-101 3.870560416648882 1102 1 0.18771173478165792 1102 2 0.1514948158818754 1102 3 0.15067620636565238 1102 4 0.07586789686379802 1102 5 -0.06329524804331754 1102 6 0.028626372619427798 1102 7 0.09558215507421153 1102 8 0.1581671532799902 1102 9 0.17439165015388625 1102 10-11 0.27575029000592366 1102 12-13 0.23206060923903493 1102 14-15 0.30673217251924 1102 16-17 0.29291171248583225 1102 18-19 0.3267503194948205 1102 20-21 0.1599100449244375 1102 22-23 0.30428815608635773 1102 24-25 0.36539598132878837 1102 26-27 0.34617146530169407 1102 28-29 0.36985969639846417 1102 30-31 0.3214316155925516 1102 32-33 0.26270360702276463 1102 34-35 0.3736089452328457 1102 36-37 0.4459139276291282 1102 38-39 0.5698457333268863 1102 40-41 0.6734355509349328 1102 42-43 0.8040168422798217 1102 44-45 0.6691396399762155 1102 46-47 0.5956490661158114 1102 48-49 0.7926982946257226 1102 50-51 0.8856312876608925 1102 52-53 0.8305915042367289 1102 54-55 1.0506707992108417 1102 56-57 1.085202651600902 1102 58-59 1.1809930992821904 1102 60-61 1.035081117042317 1102 62-63 1.0953226818217132 1102 64-65 1.1796032803029348 1102 66-67 1.4067972361411023 1102 68-69 1.538408033317456 1102 70-71 1.4749368643608811 1102 72-73 1.9188005318317707 1102 74-75 2.2388614326434997 1102 76-77 2.0650242190276913 1102 78-79 2.050740739919334 1102 80-81 2.2115469163660677 1102 82-83 2.2471448915165446 1102 84-85 2.548709376983158 1102 86-87 2.6430476565209737 1102 88-89 2.860409686232689 1102 90-91 3.068713798322811 1102 92-93 3.342374132315431 1102 94-95 3.3373407210166555 1102 96-97 3.3624121768091797 1102 98-99 3.970119949591183 1102 100-101 3.7063945155731055 1103 1 0.22005202655012823 1103 2 0.18752257818059093 1103 3 0.19588353058301067 1103 4 0.019546365722305836 1103 5 -0.022828804275185632 1103 6 0.06266733737002994 1103 7 0.13001640714959706 1103 8 0.09685933897161902 1103 9 0.189669844675727 1103 10-11 0.18651621796396967 1103 12-13 0.27299696170450005 1103 14-15 0.4030118387429624 1103 16-17 0.29600710877371483 1103 18-19 0.2826226600723558 1103 20-21 0.2845901640894226 1103 22-23 0.1945000883023411 1103 24-25 0.305284817826994 1103 26-27 0.3543988861084131 1103 28-29 0.46332187508878775 1103 30-31 0.29995780617080214 1103 32-33 0.3185748759727076 1103 34-35 0.4196408868791508 1103 36-37 0.45841035553716125 1103 38-39 0.5305348613077996 1103 40-41 0.6093631160541761 1103 42-43 0.5593898044013628 1103 44-45 0.6516178144943439 1103 46-47 0.5302374539592662 1103 48-49 0.49091564213311756 1103 50-51 0.6785655111595403 1103 52-53 0.7425889108979717 1103 54-55 0.7300117839651534 1103 56-57 0.7777973477587636 1103 58-59 1.05593058767289 1103 60-61 0.9680433100210095 1103 62-63 0.9921963869383816 1103 64-65 0.9758459753748738 1103 66-67 1.1000602707046667 1103 68-69 1.2280239619899298 1103 70-71 1.247172779602284 1103 72-73 1.4957796493821718 1103 74-75 1.338549589015397 1103 76-77 1.3690917601424708 1103 78-79 1.4411644971885416 1103 80-81 1.7204366030221578 1103 82-83 1.7987403663904509 1103 84-85 2.0058748148501877 1103 86-87 2.1935479279999655 1103 88-89 2.3233252991322253 1103 90-91 2.615574286699225 1103 92-93 2.8065796276217014 1103 94-95 2.7543176238700404 1103 96-97 2.9532724437158926 1103 98-99 3.3970145871667334 1103 100-101 3.5732705068286243 1104 1 0.21522900373614107 1104 2 0.2087108949674814 1104 3 0.21618069422568453 1104 4 0.07881947485329732 1104 5 -0.04939218857744976 1104 6 -0.0016721976076539136 1104 7 0.024309284911929296 1104 8 0.10633616153875636 1104 9 0.09994453244924273 1104 10-11 0.19992441345999623 1104 12-13 0.24969269433603358 1104 14-15 0.2206186868790141 1104 16-17 0.17734018247714545 1104 18-19 0.24466884174109538 1104 20-21 0.28801966314610894 1104 22-23 0.275376789598468 1104 24-25 0.366947085025231 1104 26-27 0.3586897338563446 1104 28-29 0.27695496174059997 1104 30-31 0.3320278943224366 1104 32-33 0.3629543687870225 1104 34-35 0.3610238068743854 1104 36-37 0.47093650480515237 1104 38-39 0.4576871484849647 1104 40-41 0.5187951657097116 1104 42-43 0.6812971370584648 1104 44-45 0.540617255826426 1104 46-47 0.6153723836853331 1104 48-49 0.783215363660176 1104 50-51 0.8212344601849821 1104 52-53 0.8998739636667565 1104 54-55 0.9401979193972494 1104 56-57 0.867860278205427 1104 58-59 1.0871789008236448 1104 60-61 1.213169536846813 1104 62-63 1.3372190267320292 1104 64-65 1.4888135617076088 1104 66-67 1.6161245397024402 1104 68-69 1.6864805284573414 1104 70-71 1.7202914811348613 1104 72-73 1.846084962886934 1104 74-75 2.0598382143446123 1104 76-77 2.046210020569145 1104 78-79 2.1277425254120352 1104 80-81 2.296952581686142 1104 82-83 2.291414597427284 1104 84-85 2.376360557890102 1104 86-87 2.581572763719471 1104 88-89 2.771080245595151 1104 90-91 3.246909761316928 1104 92-93 3.4572585233865887 1104 94-95 3.716685362470418 1104 96-97 4.001467072092446 1104 98-99 4.396728961158967 1104 100-101 4.440519486274479 1105 1 0.2786989972078189 1105 2 0.20276555964368015 1105 3 0.205407257382916 1105 4 0.16350635603257757 1105 5 0.025116037634617783 1105 6 0.07698863671983247 1105 7 0.19046234687448305 1105 8 0.1820584753892618 1105 9 0.1803294891863132 1105 10-11 0.17996165872587255 1105 12-13 0.2133197338154602 1105 14-15 0.36050154340899354 1105 16-17 0.3370065210204132 1105 18-19 0.3442248041655276 1105 20-21 0.17246295072532547 1105 22-23 0.3122312254264372 1105 24-25 0.33685363659726875 1105 26-27 0.3993610488861634 1105 28-29 0.37584893442099343 1105 30-31 0.42234223157679907 1105 32-33 0.493078735938802 1105 34-35 0.4719422450814932 1105 36-37 0.4856738089594046 1105 38-39 0.5516602013891969 1105 40-41 0.6853097221890039 1105 42-43 0.5536198813497535 1105 44-45 0.7271431491711056 1105 46-47 0.6620138683552668 1105 48-49 0.8377909467466438 1105 50-51 0.8527561922295419 1105 52-53 0.9441800913170013 1105 54-55 1.217951742407422 1105 56-57 1.1979372118130627 1105 58-59 1.2603886337647197 1105 60-61 1.2038462688618736 1105 62-63 1.3308204651356306 1105 64-65 1.543711214833678 1105 66-67 1.7061931724398391 1105 68-69 1.7327457732352265 1105 70-71 1.8629630761715994 1105 72-73 1.9341480693680992 1105 74-75 1.8005820766209446 1105 76-77 1.8995405662504723 1105 78-79 2.0485978875600033 1105 80-81 2.0808258906632204 1105 82-83 2.219898906330169 1105 84-85 2.5212809002684047 1105 86-87 2.6037746424975907 1105 88-89 2.743122548369545 1105 90-91 2.872371330367656 1105 92-93 2.958754170086152 1105 94-95 3.409468572237504 1105 96-97 3.7325165485695386 1105 98-99 3.714567854119654 1105 100-101 3.1700857332101577 1106 1 0.1195256176392192 1106 2 0.03512731045368156 1106 3 0.049958552411396795 1106 4 0.130255410018016 1106 5 -0.016264770228978875 1106 6 0.024090999439373206 1106 7 0.03717544675808426 1106 8 0.0927790540139668 1106 9 0.21727871706387702 1106 10-11 0.11957913203546866 1106 12-13 0.04336807150809108 1106 14-15 -0.0963365890531449 1106 16-17 0.004175549386900457 1106 18-19 -0.1042045432351344 1106 20-21 -0.060776538443313655 1106 22-23 -0.09269719777865504 1106 24-25 0.03065953183430281 1106 26-27 -0.16243288924266608 1106 28-29 0.043678980809218615 1106 30-31 -0.03430545331555379 1106 32-33 0.12497282549593791 1106 34-35 0.003536447197646453 1106 36-37 0.12188251070381284 1106 38-39 -0.036770003775316695 1106 40-41 0.12906305689595854 1106 42-43 0.021873771053918745 1106 44-45 0.16433219102788144 1106 46-47 0.2788488036975636 1106 48-49 0.21819932032140343 1106 50-51 0.3299924590852754 1106 52-53 0.4580310281540889 1106 54-55 0.4384435410152676 1106 56-57 0.6183995585290063 1106 58-59 0.8454207744163966 1106 60-61 0.7336121743081065 1106 62-63 0.6736218919028403 1106 64-65 0.7881776632204378 1106 66-67 0.9817289674354797 1106 68-69 0.8479143673212697 1106 70-71 0.6946845032627209 1106 72-73 1.0874673605672314 1106 74-75 1.4559276099956087 1106 76-77 1.47457750858025 1106 78-79 1.4800028717678515 1106 80-81 1.2358454137885069 1106 82-83 1.755912091900182 1106 84-85 1.9314775231381276 1106 86-87 2.026607386643125 1106 88-89 2.041324753302529 1106 90-91 2.2431902939069914 1106 92-93 2.109748351388042 1106 94-95 2.4997338222141856 1106 96-97 2.573978394123454 1106 98-99 2.891547331669482 1106 100-101 2.7738859570085452 1107 1 0.1659550386870876 1107 2 0.13530754288254343 1107 3 0.13901262305385842 1107 4 0.09371882119010877 1107 5 0.10093930910984028 1107 6 0.0697357682365336 1107 7 0.0891077899959214 1107 8 0.05585531727819415 1107 9 0.1066923996811795 1107 10-11 0.07855013493131224 1107 12-13 0.1695986977403905 1107 14-15 0.31990909360802533 1107 16-17 0.3365925920581532 1107 18-19 0.13937682773146065 1107 20-21 0.16441622070286854 1107 22-23 0.25845935709842394 1107 24-25 0.1873254359830696 1107 26-27 0.2524592987461176 1107 28-29 0.27661241360493705 1107 30-31 0.3374238550604076 1107 32-33 0.36770527339469794 1107 34-35 0.3542193474746256 1107 36-37 0.33307215134465906 1107 38-39 0.4738933167475068 1107 40-41 0.45578680173411357 1107 42-43 0.34149269689056894 1107 44-45 0.4510802491201744 1107 46-47 0.6182942105120404 1107 48-49 0.6547582364047315 1107 50-51 0.6350791744292152 1107 52-53 0.8153396100139432 1107 54-55 0.7905020195392183 1107 56-57 0.8596626110638539 1107 58-59 0.7536163087825543 1107 60-61 0.8806427864069164 1107 62-63 1.1369139649961255 1107 64-65 1.234826336088986 1107 66-67 1.3563789427780222 1107 68-69 1.4947946588122711 1107 70-71 1.6383844858700023 1107 72-73 1.7748969025417125 1107 74-75 1.6145109776219364 1107 76-77 1.4733836248470702 1107 78-79 1.507478493785996 1107 80-81 1.618442125376717 1107 82-83 2.039178103329423 1107 84-85 2.0800114936155865 1107 86-87 2.1822024658744255 1107 88-89 2.248408067471381 1107 90-91 2.218821602384679 1107 92-93 2.428292949395402 1107 94-95 2.64138609394389 1107 96-97 3.1703265933069424 1107 98-99 3.499766746756311 1107 100-101 3.133631194799907 1108 1 0.18476544890854285 1108 2 0.18569501044792247 1108 3 0.10827644262442959 1108 4 0.12055728606538452 1108 5 0.05341832293854765 1108 6 0.1304625896995475 1108 7 0.18032609225717522 1108 8 0.1586431452194006 1108 9 0.22208638992179175 1108 10-11 0.1930093408015594 1108 12-13 0.18078290094913285 1108 14-15 0.31628849244140866 1108 16-17 0.3674504867197328 1108 18-19 0.27555773385100935 1108 20-21 0.25349197112831234 1108 22-23 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-0.061861179816666834 2316 54-55 0.1675982199284647 2316 56-57 0.05480074769733534 2316 58-59 0.3638382522140873 2316 60-61 0.5006348262745632 2316 62-63 0.4111566253805492 2316 64-65 0.6176426257656757 2316 66-67 0.8691528943397202 2316 68-69 1.016882094039591 2316 70-71 1.0728653834844089 2316 72-73 1.1570257613704378 2316 74-75 1.6404231405238612 2316 76-77 1.5092435059087848 2316 78-79 1.4415063851623913 2316 80-81 1.5388035772121214 2316 82-83 1.7009801587953142 2316 84-85 1.8404072590312595 2316 86-87 1.688999749125042 2316 88-89 1.978357357048889 2316 90-91 2.357429789986192 2316 92-93 2.489045117501032 2316 94-95 2.9559734873096737 2316 96-97 3.5439033332334446 2316 98-99 3.619510193493621 2316 100-101 4.035952900071496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 11.0 5 39.0 6 71.0 7 177.0 8 469.0 9 845.0 10 1512.0 11 2670.0 12 4070.0 13 6202.0 14 7900.0 15 11555.0 16 15160.0 17 21310.0 18 22130.0 19 17570.0 20 16583.0 21 17385.0 22 18711.0 23 20556.0 24 23492.0 25 26479.0 26 29991.0 27 34799.0 28 41617.0 29 50191.0 30 59916.0 31 71733.0 32 87228.0 33 110040.0 34 143282.0 35 194033.0 36 267877.0 37 333430.0 38 186950.0 39 9123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99825833087617 19.000149316727732 15.049185576974391 22.952406775421714 2 15.460371493159245 20.190377709332186 40.24440935304897 24.1048414444596 3 19.26669351191352 23.93830552586227 30.343430488330135 26.451570473894076 4 12.737906888363607 17.071832288008366 37.12859518566535 33.061665637962676 5 13.214179317133828 37.74669490354559 34.55282973248364 14.486296046836951 6 31.962296717673333 36.50179476550536 17.08296565661357 14.452942860207738 7 28.23412216048907 33.30269735008628 20.32987676788041 18.133303721544248 8 26.39181548509444 34.932373391809556 19.51908050884232 19.156730614253686 9 26.13301479098084 16.830313128498094 20.35995573325025 36.67671634727082 10-11 24.92910150680523 26.581962215860237 27.107778107795898 21.381158169538633 12-13 26.109027085623172 23.972692827460477 27.587855644684407 22.330424442231944 14-15 22.500069402649377 25.5377222939907 26.356781366561076 25.605426936798846 16-17 21.662736545117248 28.63359255335177 27.34391613680327 22.35975476472771 18-19 21.647313889097024 26.864693709345556 29.960293609513577 21.527698792043847 20-21 22.344251063410017 25.942945916618736 30.28480144379034 21.42800157618091 22-23 21.90145392838815 26.142464713720788 30.18496996407764 21.77111139381342 24-25 21.908793499465528 26.260155063664726 30.24886947343795 21.582181963431797 26-27 21.410129069559016 26.542534194605683 30.132816174597675 21.914520561237623 28-29 21.404472062948052 27.136509449269237 29.847227964597383 21.61179052318533 30-31 21.6480146535232 26.54368969719969 29.733218982953634 22.075076666323472 32-33 21.261057927882714 26.59794503527271 29.82814561022297 22.31285142662161 34-35 22.11874005678985 26.77325410572702 29.576248753982004 21.531757083501134 36-37 22.18057737971705 26.212613302212006 29.71613466623183 21.890674651839113 38-39 21.66344966559092 26.664666654267666 29.622879063670744 22.04900461647067 40-41 22.212701035485214 26.428391406133123 29.616467489969285 21.742440068412378 42-43 21.831571122180844 26.531037458858002 29.313827942028297 22.323563476932858 44-45 21.828285444674115 26.652469381544808 29.554683042150625 21.96456213163045 46-47 22.357080442904678 26.997353805716834 28.778643072108117 21.866922679270363 48-49 22.03171012933416 27.043954640057745 28.66598171436087 22.258353516247222 50-51 22.058932132043424 27.416734362307064 28.626550239149335 21.897783266500173 52-53 22.520385506682327 27.09624244724187 28.46920167299421 21.91417037308159 54-55 21.87681423867066 27.20709798896781 28.698594212656133 22.2174935597054 56-57 22.210443983017775 27.260182112601044 28.289012654734258 22.240361249646924 58-59 22.210809345178767 27.128315895978528 28.19676602607814 22.464108732764565 60-61 21.80978342605884 27.309625215013394 28.70606004904273 22.174531309885037 62-63 21.858621316067936 27.257148740483757 28.781284414945258 22.102945528503053 64-65 21.504169278810988 27.398433629792624 28.51725794114334 22.580139150253057 66-67 21.60599208135345 27.390512178490283 28.335062785865027 22.668432954291244 68-69 22.171398230279312 26.996326333691805 28.49023904436161 22.34203639166727 70-71 22.004838508837988 27.119098148526483 28.096556713134536 22.779506629500997 72-73 21.92765843214117 27.303117433087742 28.183412789281103 22.585811345489986 74-75 21.924034980003956 27.775040603908003 27.839376168726826 22.46154824736122 76-77 21.92247173628402 27.140201938745644 28.340153208665548 22.597173116304795 78-79 21.93505854347731 27.439778730937185 28.150299954126883 22.47486277145862 80-81 22.321781841978446 27.437127853059167 27.843194542222204 22.397895762740188 82-83 22.11922543251644 27.47170732811737 28.098097434388063 22.310969804978132 84-85 22.037969238200176 27.63192792777252 27.919255127146332 22.410847706880972 86-87 22.11945508952016 27.718878719090572 27.61106532036482 22.550600871024447 88-89 21.997157052627948 27.80568557131437 27.311080648821612 22.88607672723607 90-91 21.988641876812608 27.65177406660701 27.648458809957628 22.71112524662275 92-93 22.19518289013116 27.40957239073665 27.777716386005945 22.61752833312625 94-95 21.836355158575074 27.259630804559286 27.919940273244514 22.984073763621122 96-97 21.9805451466499 26.836091876759156 28.10356012873592 23.079802847855028 98-99 21.778006454579415 27.125836268008975 27.598609683378463 23.49754759403315 100-101 22.124426653413234 26.607890499628866 27.880586507481738 23.387096339476162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 772.0 1 517.0 2 359.5 3 458.5 4 384.0 5 370.0 6 714.5 7 1596.0 8 2589.0 9 2980.5 10 2470.0 11 1863.5 12 1766.5 13 1845.5 14 2040.5 15 2172.5 16 2324.5 17 2586.5 18 2817.5 19 3122.5 20 3591.5 21 4260.5 22 5090.0 23 6289.0 24 7826.5 25 9690.5 26 12293.0 27 15394.0 28 18149.0 29 20785.5 30 23820.0 31 28121.5 32 34282.0 33 37158.5 34 37714.5 35 40287.5 36 43690.5 37 47041.0 38 49208.0 39 50826.5 40 53153.5 41 55869.5 42 57974.0 43 59792.5 44 61604.0 45 64556.5 46 66517.0 47 69126.5 48 72939.5 49 71681.5 50 67615.0 51 64682.5 52 62591.5 53 62175.0 54 62351.5 55 58976.5 56 53376.5 57 47759.0 58 43646.5 59 38298.0 60 30228.0 61 24878.5 62 21270.0 63 15828.0 64 10662.5 65 7681.0 66 5630.5 67 4311.5 68 3265.0 69 2356.5 70 1689.5 71 1154.0 72 792.5 73 587.0 74 418.5 75 287.5 76 207.0 77 141.5 78 80.0 79 35.0 80 19.5 81 7.0 82 6.0 83 4.5 84 1.5 85 0.0 86 0.0 87 1.0 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014985577729059677 3 0.0021561982343970758 4 0.0017249585875176608 5 0.11972290696489762 6 0.0 7 0.0 8 2.156198234397076E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.695247792996345E-5 16-17 2.695247792996345E-5 18-19 0.0 20-21 0.0 22-23 2.6952477929963447E-4 24-25 4.312396468794152E-4 26-27 0.005902592666661995 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011212230818864794 36-37 6.468594703191227E-4 38-39 0.0069267868280006055 40-41 0.010214989135456147 42-43 0.005848687710802068 44-45 0.0036385845205450656 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.39049558599269E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.078099117198538E-4 68-69 0.0011859090289183917 70-71 0.0 72-73 5.39049558599269E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6171486757978067E-4 82-83 0.0023179131019768562 84-85 0.004797541071533494 86-87 0.003234297351595614 88-89 0.0 90-91 0.003072582484015833 92-93 2.695247792996345E-5 94-95 1.078099117198538E-4 96-97 8.085743378989034E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1855117.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.20516919698109 #Duplication Level Percentage of deduplicated Percentage of total 1 77.67667249323021 45.98860537620181 2 13.241889419423469 15.679766071293603 3 4.227962636831683 7.509517298164023 4 1.7639183952886943 4.17732348170938 5 0.902989282024437 2.673081661265863 6 0.5444586398055237 1.9340859534266526 7 0.3418780706066534 1.4168664310503054 8 0.23241584453078698 1.1008175519603596 9 0.16508567926750015 0.8796533015727809 >10 0.8228726365931605 8.651805088215719 >50 0.0461843413889394 1.876008212988507 >100 0.02703019547752179 3.1084482704291148 >500 0.0036396523458926008 1.4413874122477714 >1k 0.0028207305680667647 2.679948407724991 >5k 1.8198261729462998E-4 0.8826854817491302 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8877 0.478514293168571 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7508 0.40471840859633107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4719 0.254377486702995 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2459 0.13255228645956024 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2333 0.12576026202120943 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2271 0.12241815475789398 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2263 0.12198691511101456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2263 0.12198691511101456 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2235 0.12047757634693661 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1987 0.10710914729367474 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.390495585992689E-5 2 0.0 0.0 0.0 0.0 5.390495585992689E-5 3 0.0 0.0 0.0 0.0 5.390495585992689E-5 4 0.0 0.0 0.0 0.0 5.390495585992689E-5 5 0.0 0.0 0.0 0.0 5.390495585992689E-5 6 0.0 0.0 0.0 0.0 5.390495585992689E-5 7 0.0 0.0 0.0 0.0 5.390495585992689E-5 8 0.0 0.0 0.0 0.0 1.0780991171985379E-4 9 0.0 0.0 0.0 0.0 1.0780991171985379E-4 10-11 0.0 0.0 0.0 5.390495585992689E-5 1.0780991171985379E-4 12-13 2.6952477929963446E-5 0.0 0.0 1.0780991171985379E-4 1.0780991171985379E-4 14-15 5.390495585992689E-5 0.0 0.0 1.0780991171985379E-4 1.3476238964981724E-4 16-17 5.390495585992689E-5 0.0 0.0 1.0780991171985379E-4 1.6171486757978067E-4 18-19 5.390495585992689E-5 0.0 0.0 1.0780991171985379E-4 2.6952477929963447E-4 20-21 5.390495585992689E-5 0.0 0.0 1.8866734550974413E-4 2.6952477929963447E-4 22-23 5.390495585992689E-5 0.0 0.0 3.503822130895248E-4 3.7733469101948827E-4 24-25 5.390495585992689E-5 0.0 0.0 8.355268158288669E-4 4.3123964687941514E-4 26-27 5.390495585992689E-5 0.0 0.0 0.0017788635433775876 4.3123964687941514E-4 28-29 5.390495585992689E-5 0.0 0.0 0.004878398505323384 4.3123964687941514E-4 30-31 1.0780991171985379E-4 0.0 0.0 0.015524627287658946 4.3123964687941514E-4 32-33 1.0780991171985379E-4 0.0 0.0 0.03751784927850912 5.390495585992689E-4 34-35 1.0780991171985379E-4 0.0 0.0 0.07013034757376489 5.390495585992689E-4 36-37 1.0780991171985379E-4 0.0 0.0 0.12584111945499935 5.390495585992689E-4 38-39 1.0780991171985379E-4 0.0 0.0 0.2090434188247965 5.390495585992689E-4 40-41 1.0780991171985379E-4 0.0 0.0 0.29814831086125565 5.390495585992689E-4 42-43 1.3476238964981724E-4 0.0 0.0 0.38315642625236035 5.390495585992689E-4 44-45 1.6171486757978067E-4 0.0 0.0 0.4885945199143774 5.390495585992689E-4 46-47 1.6171486757978067E-4 0.0 0.0 0.6084791417468548 5.660020365292325E-4 48-49 1.6171486757978067E-4 0.0 0.0 0.7325683501364064 6.199069923891593E-4 50-51 1.6171486757978067E-4 0.0 0.0 0.8628027234939899 6.468594703191227E-4 52-53 1.6171486757978067E-4 0.0 0.0 0.9925789047267639 6.468594703191227E-4 54-55 1.6171486757978067E-4 0.0 0.0 1.1279342488910404 6.468594703191227E-4 56-57 1.6171486757978067E-4 0.0 0.0 1.2800270818498238 6.468594703191227E-4 58-59 1.6171486757978067E-4 0.0 0.0 1.4382381272987095 7.007644261790496E-4 60-61 1.6171486757978067E-4 0.0 0.0 1.6094941720656972 7.007644261790496E-4 62-63 1.6171486757978067E-4 0.0 0.0 1.7753597212466923 7.007644261790496E-4 64-65 1.6171486757978067E-4 0.0 0.0 1.9461575738888706 7.007644261790496E-4 66-67 1.6171486757978067E-4 0.0 0.0 2.13816702666193 7.546693820389765E-4 68-69 1.6171486757978067E-4 0.0 0.0 2.351603699389311 8.085743378989035E-4 70-71 2.1561982343970757E-4 0.0 0.0 2.5685441942475866 8.085743378989035E-4 72-73 2.1561982343970757E-4 0.0 0.0 2.787829554685769 9.163842496187572E-4 74-75 2.1561982343970757E-4 0.0 0.0 3.0066567229991423 9.702892054786841E-4 76-77 2.1561982343970757E-4 0.0 0.0 3.2439732911724706 9.702892054786841E-4 78-79 2.1561982343970757E-4 0.0 0.0 3.5100212008191396 9.972416834086477E-4 80-81 2.1561982343970757E-4 0.0 0.0 3.7903000188128297 0.0010780991171985379 82-83 2.1561982343970757E-4 0.0 0.0 4.070794456629959 0.0011320040730584647 84-85 2.42572301369671E-4 2.6952477929963446E-5 0.0 4.352771280733236 0.0011320040730584647 86-87 2.6952477929963447E-4 5.390495585992689E-5 0.0 4.647766151676686 0.0011859090289183917 88-89 2.6952477929963447E-4 1.0780991171985379E-4 0.0 4.959282891591204 0.0012398139847783186 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6510 0.0 34.119217 1 GTATCAA 9875 0.0 32.70362 1 TAAGGTG 1145 0.0 28.240528 5 ATCAACG 12000 0.0 26.511707 3 TCAACGC 12110 0.0 26.348007 4 TATCAAC 12120 0.0 26.331953 2 GTAAGGT 1235 0.0 26.182516 4 CAACGCA 12205 0.0 26.142921 5 GTGTAGC 1295 0.0 25.70937 1 AACGCAG 12560 0.0 25.448828 6 GGTAAGG 1290 0.0 24.330278 3 AGGTAAG 1205 0.0 24.0772 2 AAGGTAA 1220 0.0 23.781168 1 TATACCG 100 0.001643513 23.776033 5 ACGCAGA 13865 0.0 22.779497 7 TACATGG 6750 0.0 22.759459 2 GTACATG 6890 0.0 22.71119 1 CGCAGAG 14010 0.0 22.475933 8 GAGTACT 8380 0.0 22.386976 12-13 ACATGGG 6690 0.0 21.893614 3 >>END_MODULE