FastQCFastQC Report
Thu 26 May 2016
SRR938074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812775
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86780.478713574492146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71140.39243700955717065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.25099640054612404No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24410.13465543158968984No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23650.13046296423990844No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22040.12158155314366097No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21310.11755457792610777No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20970.11567900042752134No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19820.10933513535877316No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19180.1058046365379046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63300.034.897281
GTATCAA94800.032.209651
TATCAAC112750.026.7663572
ATCAACG112600.026.7598083
CAACGCA114100.026.491325
TCAACGC114450.026.4103074
TAAGGTG11750.026.291035
AACGCAG117450.025.8123666
GAGTACT75200.023.12083612-13
ACGCAGA130500.023.042077
CGCAGAG130600.022.8061878
TAGCCCT13100.022.4932674
GTACATG68400.022.3904721
GTAAGGT11800.022.1519934
TACATGG68300.022.0582312
CGAATAC1100.00286884221.602773
GTAGCCC9750.021.4476743
GTATAGC9250.021.1473751
GTACTTT81450.021.14400114-15
GCAGAGT137300.020.9667159
Produced by FastQC (version 0.11.5)