##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938074_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1812775 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.722710209485456 33.0 31.0 34.0 30.0 34.0 2 31.9222479347961 33.0 31.0 34.0 30.0 34.0 3 31.99487857014798 34.0 31.0 34.0 30.0 34.0 4 35.53068086221401 37.0 35.0 37.0 33.0 37.0 5 35.533713229716874 37.0 35.0 37.0 33.0 37.0 6 35.5519934906428 37.0 35.0 37.0 33.0 37.0 7 35.493230544331205 37.0 35.0 37.0 33.0 37.0 8 35.45840989642951 37.0 35.0 37.0 33.0 37.0 9 37.080337052309304 39.0 37.0 39.0 33.0 39.0 10-11 36.981179683082566 39.0 37.0 39.0 33.0 39.0 12-13 36.879446704637914 39.0 37.0 39.0 33.0 39.0 14-15 38.02819517038794 40.0 38.0 41.0 33.0 41.0 16-17 37.87783425962957 40.0 38.0 41.0 33.0 41.0 18-19 38.033885893174826 40.0 38.0 41.0 33.0 41.0 20-21 38.01227675800913 40.0 38.0 41.0 33.0 41.0 22-23 37.91761553419481 40.0 38.0 41.0 33.0 41.0 24-25 37.79781274565238 40.0 38.0 41.0 32.5 41.0 26-27 37.62048323702611 40.0 37.5 41.0 32.0 41.0 28-29 37.41252306546593 40.0 37.0 41.0 31.5 41.0 30-31 37.1789714663982 40.0 37.0 41.0 31.0 41.0 32-33 36.90153521534664 39.5 36.0 41.0 30.5 41.0 34-35 36.650011998179586 39.0 36.0 41.0 30.0 41.0 36-37 36.37681124243218 39.0 35.0 41.0 30.0 41.0 38-39 36.064958420101775 39.0 35.0 40.0 29.5 41.0 40-41 35.840761539628474 39.0 35.0 40.0 28.5 41.0 42-43 35.64211719601164 39.0 35.0 40.0 27.0 41.0 44-45 35.358442719035736 39.0 35.0 40.0 26.5 41.0 46-47 34.9461916123071 38.0 34.5 40.0 24.5 41.0 48-49 34.52405924618334 38.0 34.0 40.0 23.5 41.0 50-51 33.82614913599316 37.5 33.5 39.5 22.5 40.5 52-53 33.963239784308584 38.0 33.5 40.0 23.0 40.5 54-55 34.06289969797686 38.0 34.0 40.0 22.0 41.0 56-57 33.74620981644164 38.0 33.0 40.0 19.5 41.0 58-59 33.417531960668036 37.5 33.0 40.0 18.5 41.0 60-61 32.98708913130422 37.0 32.5 40.0 14.5 41.0 62-63 32.44810359807477 36.5 31.5 39.5 9.5 41.0 64-65 31.92565817600088 36.0 31.0 39.0 7.5 40.5 66-67 31.386069975589912 35.0 31.0 39.0 4.0 40.0 68-69 30.770144116065147 35.0 30.0 38.0 2.0 40.0 70-71 30.20949124960351 35.0 29.0 37.5 2.0 40.0 72-73 29.56185020203831 34.0 28.5 37.0 2.0 39.0 74-75 28.867981961357586 34.0 27.5 36.0 2.0 39.0 76-77 28.18917074650743 34.0 26.0 36.0 2.0 38.5 78-79 27.44807987753582 33.0 25.5 35.0 2.0 37.5 80-81 26.778714126132584 33.0 24.5 35.0 2.0 37.0 82-83 26.0321531905504 32.0 22.0 35.0 2.0 36.0 84-85 25.3665002551337 32.0 19.5 35.0 2.0 36.0 86-87 24.67067424252872 31.0 16.5 34.0 2.0 35.0 88-89 23.842405428141937 31.0 7.5 34.0 2.0 35.0 90-91 22.989386713739982 30.0 2.0 34.0 2.0 35.0 92-93 22.078207168567527 29.5 2.0 34.0 2.0 35.0 94-95 21.117095337259173 29.0 2.0 34.0 2.0 35.0 96-97 19.911274978968706 28.0 2.0 34.0 2.0 35.0 98-99 18.42066169270869 25.5 2.0 33.0 2.0 35.0 100-101 14.87641792279792 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3789428619075039 1101 2 0.43883227359306076 1101 3 0.4035105035032913 1101 4 0.38330068829306896 1101 5 0.43312702668674774 1101 6 0.3461666223170212 1101 7 0.3877865229858415 1101 8 0.2532879884033079 1101 9 0.40050556001242654 1101 10-11 0.43334856708668923 1101 12-13 0.4808063369320479 1101 14-15 0.690870588737674 1101 16-17 0.7629867006080033 1101 18-19 0.6453614757723969 1101 20-21 0.7328286747268891 1101 22-23 0.8316772441339211 1101 24-25 0.7719001868435456 1101 26-27 0.739266145844951 1101 28-29 0.7626048546792319 1101 30-31 0.7901513371257849 1101 32-33 0.8767100911285368 1101 34-35 0.8578770293572617 1101 36-37 0.9551367979794847 1101 38-39 1.03369032178815 1101 40-41 1.0820861009483593 1101 42-43 1.2248565135991356 1101 44-45 1.2077458488401263 1101 46-47 1.3165776738720112 1101 48-49 1.4454834980144113 1101 50-51 1.4790601114478577 1101 52-53 1.487296614718467 1101 54-55 1.485653141828827 1101 56-57 1.5134810856578653 1101 58-59 1.6218525045673076 1101 60-61 1.5076214361819424 1101 62-63 1.6006780306064883 1101 64-65 1.6034256949404018 1101 66-67 1.6739395149438643 1101 68-69 1.7808299664893248 1101 70-71 2.239055080778538 1101 72-73 2.3273106613599097 1101 74-75 2.5870455034461095 1101 76-77 2.7054257472478156 1101 78-79 2.8323593544904213 1101 80-81 2.8159208112223375 1101 82-83 3.009736300255909 1101 84-85 3.188737107054198 1101 86-87 3.2579560069933144 1101 88-89 3.494136334041478 1101 90-91 3.764854316877173 1101 92-93 4.219596907818353 1101 94-95 4.591888364006831 1101 96-97 4.83244470872523 1101 98-99 5.228836228207694 1101 100-101 5.0809606700274 1102 1 0.424780930262898 1102 2 0.3200256484995485 1102 3 0.15084802945256826 1102 4 0.10193753144876894 1102 5 0.16803265888517416 1102 6 0.28440292673243306 1102 7 0.10613010025936376 1102 8 0.33822444067360635 1102 9 0.44234889900742047 1102 10-11 0.3651226695369516 1102 12-13 0.2945266658242929 1102 14-15 0.5146108352710996 1102 16-17 0.5778337610927338 1102 18-19 0.4580706483417245 1102 20-21 0.5310220346985375 1102 22-23 0.5526191435464369 1102 24-25 0.34904303040928397 1102 26-27 0.4918480057208825 1102 28-29 0.5287366844818351 1102 30-31 0.5268673091188916 1102 32-33 0.7386439759646564 1102 34-35 0.624945511013884 1102 36-37 0.7543513274031497 1102 38-39 0.5863003202539332 1102 40-41 0.7157808912700432 1102 42-43 0.9902982860655456 1102 44-45 0.963510865478888 1102 46-47 0.9618160745953972 1102 48-49 1.09783088095584 1102 50-51 0.9727172184613906 1102 52-53 0.901391943780439 1102 54-55 1.036711953857619 1102 56-57 1.1938560654618868 1102 58-59 1.1472395204919152 1102 60-61 1.2729479728069109 1102 62-63 1.3020726049161269 1102 64-65 1.1614707859275093 1102 66-67 1.3080122584597795 1102 68-69 1.5754009497310797 1102 70-71 1.6460799527123164 1102 72-73 1.7906105067164653 1102 74-75 1.7204923415375468 1102 76-77 1.8411795084506366 1102 78-79 2.299641709795612 1102 80-81 2.4779734619521747 1102 82-83 2.5805045143074175 1102 84-85 2.5084393471439164 1102 86-87 2.8782615465957484 1102 88-89 3.2678151780308227 1102 90-91 3.4901429325943205 1102 92-93 3.7170174239391542 1102 94-95 3.6452379763120497 1102 96-97 3.8690177475435874 1102 98-99 4.096004094413281 1102 100-101 4.087349216093903 1103 1 0.5677815387065266 1103 2 0.5378092126707195 1103 3 0.5051895691420683 1103 4 0.25583942154946016 1103 5 0.39418559413499565 1103 6 0.4409234142724259 1103 7 0.50778467438154 1103 8 0.4511594287991443 1103 9 0.5370174935026455 1103 10-11 0.6459921878196937 1103 12-13 0.6982151099750595 1103 14-15 0.8045726603402912 1103 16-17 0.8439616062209296 1103 18-19 0.8578614549547794 1103 20-21 0.8181779859968756 1103 22-23 0.962445181008249 1103 24-25 1.0257452005248808 1103 26-27 1.0254800543440297 1103 28-29 1.08873217022267 1103 30-31 1.1264917750951113 1103 32-33 1.1859310043390607 1103 34-35 1.1769829793650004 1103 36-37 1.2356989319017018 1103 38-39 1.4797556258169422 1103 40-41 1.44809268264585 1103 42-43 1.4412768662329043 1103 44-45 1.5688975543024952 1103 46-47 1.713000249827168 1103 48-49 1.8006037155834846 1103 50-51 1.8062081147053988 1103 52-53 1.8364675739775649 1103 54-55 1.7554400776922563 1103 56-57 1.9265571299111173 1103 58-59 2.0001098369480204 1103 60-61 2.0119240210636846 1103 62-63 2.229958132462926 1103 64-65 2.4189389407731348 1103 66-67 2.546567139420727 1103 68-69 2.4498440275399 1103 70-71 2.6776691355005617 1103 72-73 2.971059839064079 1103 74-75 2.825690118428465 1103 76-77 3.1245915687192714 1103 78-79 3.507260012826709 1103 80-81 3.3820217383556823 1103 82-83 3.509853087081858 1103 84-85 3.3922732289488344 1103 86-87 3.425741412345605 1103 88-89 3.606939084631449 1103 90-91 3.8276852128840204 1103 92-93 4.210930534263724 1103 94-95 4.528273684756332 1103 96-97 4.8083868307564295 1103 98-99 5.166909857617487 1103 100-101 4.838562014345774 1104 1 0.2191096923023501 1104 2 0.18989171936839355 1104 3 0.21981802140328455 1104 4 0.08861294952281185 1104 5 0.14262536738306153 1104 6 0.09250763268263995 1104 7 0.03133879248816385 1104 8 0.033899891346010236 1104 9 0.20472687454861926 1104 10-11 0.19100884356708292 1104 12-13 0.1950154532403232 1104 14-15 0.3578656692325737 1104 16-17 0.389434641750114 1104 18-19 0.23219437919959063 1104 20-21 0.2057962910169806 1104 22-23 0.34151338191070124 1104 24-25 0.38718876014876713 1104 26-27 0.42590621386395355 1104 28-29 0.4368384440413067 1104 30-31 0.4056443312832698 1104 32-33 0.36339353275226216 1104 34-35 0.5066341314259759 1104 36-37 0.49874785642948183 1104 38-39 0.7673510157275203 1104 40-41 0.6995490993378866 1104 42-43 0.6654289302844774 1104 44-45 0.7826974484931313 1104 46-47 0.8011484475307284 1104 48-49 0.8984896813861383 1104 50-51 1.044127699588195 1104 52-53 1.0265430246670135 1104 54-55 1.0788403262212256 1104 56-57 1.2308943911095298 1104 58-59 1.2705042844170649 1104 60-61 1.1833582608510795 1104 62-63 1.1506845414345719 1104 64-65 1.2294336764496556 1104 66-67 1.23983340376574 1104 68-69 1.3234864226456047 1104 70-71 1.5958264377308602 1104 72-73 1.6634264162234054 1104 74-75 1.8702373220161412 1104 76-77 1.6862705080419076 1104 78-79 1.989691121395115 1104 80-81 2.1042767696832527 1104 82-83 2.265584555832721 1104 84-85 2.28629276984692 1104 86-87 2.3911134522209743 1104 88-89 2.6511913907527074 1104 90-91 2.7499368607726 1104 92-93 2.9597909304951884 1104 94-95 3.1813093215964443 1104 96-97 3.4778875120580075 1104 98-99 3.665174691690563 1104 100-101 3.603528349127833 1105 1 0.489476058336777 1105 2 0.4455880151785401 1105 3 0.32914061441677944 1105 4 0.2544500278057882 1105 5 0.3727408649691384 1105 6 0.3621019806584087 1105 7 0.35169116893361263 1105 8 0.3510599932685139 1105 9 0.5136216049064899 1105 10-11 0.46140860039003684 1105 12-13 0.40925650940248204 1105 14-15 0.6299266177842853 1105 16-17 0.7070789101061266 1105 18-19 0.5400485259325265 1105 20-21 0.6247165480569663 1105 22-23 0.578123100011851 1105 24-25 0.5612753781569992 1105 26-27 0.5842919649782345 1105 28-29 0.504830437160507 1105 30-31 0.5356107792978477 1105 32-33 0.6174372723824106 1105 34-35 0.6839055398148943 1105 36-37 0.7028930925426593 1105 38-39 0.8082481110743416 1105 40-41 1.0252408355782947 1105 42-43 0.9978417141205753 1105 44-45 1.0539765920309279 1105 46-47 1.1315776458178988 1105 48-49 1.2395800403140882 1105 50-51 1.1910912103647817 1105 52-53 1.1128127324285302 1105 54-55 1.2227356265253135 1105 56-57 1.2712762320473274 1105 58-59 1.217745975004199 1105 60-61 1.4133859636914892 1105 62-63 1.5478840905437892 1105 64-65 1.5704117732437943 1105 66-67 1.7128268764422891 1105 68-69 1.9577778413280988 1105 70-71 2.0199432801597332 1105 72-73 2.0553070430030473 1105 74-75 2.1887120423107156 1105 76-77 2.429260457414564 1105 78-79 2.5803943198872332 1105 80-81 2.6972263622580606 1105 82-83 2.696615018594507 1105 84-85 2.834753454496255 1105 86-87 3.084911861668033 1105 88-89 3.27086426465182 1105 90-91 3.4305228806251513 1105 92-93 3.580413139054052 1105 94-95 3.634644543236437 1105 96-97 3.9710078582168613 1105 98-99 4.05373322286432 1105 100-101 3.8600548090249216 1106 1 0.06553060135162525 1106 2 0.11297985739242122 1106 3 -0.008648042724185956 1106 4 0.08050527853912115 1106 5 0.09450049093142354 1106 6 -0.050637744826076414 1106 7 0.008498939569413722 1106 8 0.07069165127175836 1106 9 0.10143899541819223 1106 10-11 0.17090488033856133 1106 12-13 0.19139726158833525 1106 14-15 0.36803185156067997 1106 16-17 0.03139685348751442 1106 18-19 0.1235026904029084 1106 20-21 0.13601994470940326 1106 22-23 0.07155419852116296 1106 24-25 0.007603847860131907 1106 26-27 0.2081731328156451 1106 28-29 0.0834156399558239 1106 30-31 0.24088820777722475 1106 32-33 0.12942786368969905 1106 34-35 0.06560430764869096 1106 36-37 0.291266608244662 1106 38-39 0.1284706290914457 1106 40-41 0.41333341534807744 1106 42-43 0.11368702157896138 1106 44-45 0.32416114802816054 1106 46-47 0.5208661282939389 1106 48-49 0.6714861979745237 1106 50-51 0.5596092874766541 1106 52-53 0.5091892986803259 1106 54-55 0.13332247235987893 1106 56-57 0.43039767597422696 1106 58-59 0.7514771121832524 1106 60-61 0.831301517588777 1106 62-63 0.6513600357584508 1106 64-65 0.8675158174869573 1106 66-67 0.9302612100743737 1106 68-69 0.5879683195136636 1106 70-71 0.7578163822710557 1106 72-73 1.0561203530930179 1106 74-75 1.2382837593886435 1106 76-77 1.5953571747897328 1106 78-79 1.851920568599894 1106 80-81 1.9882789887712384 1106 82-83 1.9939023619268639 1106 84-85 2.085385164021414 1106 86-87 1.8810981365719392 1106 88-89 2.330926068292211 1106 90-91 2.6552557735122413 1106 92-93 2.7436063705476386 1106 94-95 2.812718546811265 1106 96-97 2.924943452437123 1106 98-99 3.3637375597632335 1106 100-101 3.248144133289644 1107 1 0.2620266066879111 1107 2 0.08270605719834734 1107 3 0.11597119122819066 1107 4 -0.0017448542503331055 1107 5 0.11319999147836768 1107 6 0.06792079988382227 1107 7 -0.004522250471175937 1107 8 0.12620985955425823 1107 9 0.13150033248106752 1107 10-11 0.06026937127112575 1107 12-13 0.061222967705717224 1107 14-15 0.15732250194766095 1107 16-17 0.20035939382187706 1107 18-19 0.07889490310760294 1107 20-21 0.2098814895689003 1107 22-23 0.17895005658811414 1107 24-25 0.23527497469705594 1107 26-27 0.18056500376762585 1107 28-29 0.2987511409254964 1107 30-31 0.29754106251036916 1107 32-33 0.21122630645023577 1107 34-35 0.18926896726161857 1107 36-37 0.3295534801368092 1107 38-39 0.31998116234920104 1107 40-41 0.2706255809759881 1107 42-43 0.5402693434935415 1107 44-45 0.4356145303506267 1107 46-47 0.5998749630818949 1107 48-49 0.5605547777243629 1107 50-51 0.4542975719466753 1107 52-53 0.5376739067695695 1107 54-55 0.6103875714475819 1107 56-57 0.6514014681940665 1107 58-59 0.6151148982295851 1107 60-61 0.6887826765833935 1107 62-63 0.6904857801686006 1107 64-65 0.9038870899462133 1107 66-67 1.1086142639573744 1107 68-69 1.1729861002621433 1107 70-71 1.3036038340448037 1107 72-73 1.4550944087094955 1107 74-75 1.5764509492068832 1107 76-77 1.6342693580680034 1107 78-79 1.8924350093675635 1107 80-81 2.229168122270277 1107 82-83 2.3792397981227325 1107 84-85 2.3750912704149307 1107 86-87 2.517157988588899 1107 88-89 2.734469179230082 1107 90-91 3.001155358605235 1107 92-93 3.2409235431409904 1107 94-95 3.42228844622068 1107 96-97 3.530546687147119 1107 98-99 3.622701019673169 1107 100-101 3.7814474546044448 1108 1 0.2880535018835815 1108 2 0.16710969860774227 1108 3 0.12475501758866869 1108 4 0.10853985108433761 1108 5 0.15210350493175184 1108 6 0.17212045755327665 1108 7 0.07837343901881155 1108 8 0.1542184653995946 1108 9 0.21228005396134364 1108 10-11 0.2792337377731471 1108 12-13 0.2620171951536321 1108 14-15 0.3490463844544607 1108 16-17 0.39037946812639035 1108 18-19 0.30148730470685337 1108 20-21 0.23283396837899772 1108 22-23 0.24485267370643982 1108 24-25 0.27451760372364475 1108 26-27 0.3217443337925374 1108 28-29 0.40405006288004586 1108 30-31 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-1.4292034878786666 2316 52-53 -1.5774516315368103 2316 54-55 -1.3927502210496456 2316 56-57 -1.2133857242000232 2316 58-59 -1.2546357989238999 2316 60-61 -1.4593662082877756 2316 62-63 -1.6731188169267313 2316 64-65 -1.614523970246541 2316 66-67 -1.7895386118878527 2316 68-69 -1.7890503607701156 2316 70-71 -1.9492038478834317 2316 72-73 -1.94334087606234 2316 74-75 -2.1884816920197565 2316 76-77 -2.4113460818070216 2316 78-79 -3.122294345577 2316 80-81 -2.961007873351054 2316 82-83 -3.063863608648745 2316 84-85 -2.7959834165469992 2316 86-87 -2.8105173260328904 2316 88-89 -2.8843616300761106 2316 90-91 -3.362563266320489 2316 92-93 -3.7435248345597287 2316 94-95 -3.804664818839548 2316 96-97 -4.09081325943718 2316 98-99 -4.2700811453605745 2316 100-101 -4.033392380638009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5998.0 3 1917.0 4 1918.0 5 2163.0 6 2484.0 7 3079.0 8 3799.0 9 4705.0 10 5815.0 11 7223.0 12 9114.0 13 11098.0 14 12977.0 15 15771.0 16 19028.0 17 23180.0 18 22244.0 19 19031.0 20 18976.0 21 19756.0 22 21556.0 23 23838.0 24 27268.0 25 30078.0 26 34113.0 27 39288.0 28 44316.0 29 51503.0 30 60610.0 31 73113.0 32 88596.0 33 111109.0 34 143500.0 35 190315.0 36 245030.0 37 268749.0 38 141318.0 39 8199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.800659863338566 18.569616247291908 14.645518820736758 22.98420506863277 2 15.300094700523392 19.805709404048944 40.647329952958785 24.246865942468883 3 19.31659812483683 23.94119845081298 29.976835050880652 26.76536837346954 4 12.545151262248247 16.65639999823369 37.40025876420223 33.39818997531584 5 13.39547523203832 37.62711658534996 34.376233622326474 14.601174560285243 6 31.998723544747154 36.23664332019703 17.259916544560543 14.504716590495267 7 28.27504144783754 33.297459705637564 20.358118146757356 18.06938069976754 8 26.52184326446459 34.54316281154011 19.577814249748627 19.35717967424668 9 26.413852060794273 16.742156365500758 20.39143529032582 36.45255628337915 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26.752837893851428 29.474191406536747 21.578755935333497 36-37 22.265522057206375 26.235275580208437 29.551107158261143 21.948095204324048 38-39 21.71420862103548 26.701002854696842 29.484242608674784 22.1005459155929 40-41 22.2645557180843 26.463434619210975 29.48355403461513 21.7884556280896 42-43 21.839048431470136 26.60571947554921 29.213185869712753 22.342046223267896 44-45 21.907136153705675 26.6950730564308 29.432558180593354 21.965232609270164 46-47 22.417685621024848 26.99384116479043 28.698549805782736 21.889923408401984 48-49 22.070501208145977 27.03388712898276 28.60776005487008 22.287851608001187 50-51 22.083189653983343 27.406378535546498 28.550884559044626 21.95954725142553 52-53 22.517307700795815 27.100289767435704 28.46341603365098 21.918986498117505 54-55 21.875506039647853 27.15026332682568 28.723438004746534 22.250792628779937 56-57 22.187929568136006 27.293161089068242 28.30662120100734 22.212288141788417 58-59 22.260707791176003 27.064400125350897 28.240088893204568 22.434803190268536 60-61 21.73281547548603 27.234130948075453 28.792449405271764 22.24060417116675 62-63 21.904323413378766 27.151492848380254 28.87765266761251 22.06653107062847 64-65 21.622663203990573 27.271553586236692 28.608816361751444 22.496966848021295 66-67 21.590646410781368 27.259022767734244 28.462674520539178 22.68765630094521 68-69 22.203151426075333 26.857988865928533 28.629697091574858 22.30916261642128 70-71 21.950087079873747 26.947613862486907 28.269964436982338 22.832334620657008 72-73 21.90225412493 27.06475471115145 28.44180958975533 22.591181574163222 74-75 21.847170111643 27.568022064326513 28.183022683630536 22.401785140399948 76-77 21.9842612734532 26.969037277412156 28.339169336405902 22.70753211272874 78-79 22.06264456677209 27.273296543086932 28.1147182594471 22.54934063069388 80-81 22.389321280499562 27.2839550788443 27.81880693387374 22.507916706782392 82-83 22.241569177872083 27.282766055582407 28.03271805600613 22.44294671053938 84-85 22.119414472132533 27.466156152718334 27.9257014624404 22.488727912708736 86-87 22.273695313441706 27.496965171672947 27.578307673254148 22.651031841631198 88-89 22.186109264077388 27.39299696779908 27.46081452807752 22.96007924004602 90-91 22.226658315651807 27.344625597699977 27.574600406698103 22.854115679950116 92-93 22.46850947612348 27.06179480813264 27.760076140031448 22.709619575712434 94-95 22.10699330046798 26.87955107258456 27.930158875562423 23.08329675138503 96-97 22.335619894656134 26.469280959555647 27.99067239691092 23.204426748877296 98-99 22.190329338170237 26.728112887566823 27.502646474829312 23.578911299433624 100-101 22.946189409808014 25.647284747833037 27.805641793279364 23.600884049079585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 735.0 1 515.5 2 365.5 3 489.0 4 436.5 5 390.0 6 761.0 7 1609.0 8 2591.0 9 2966.5 10 2408.5 11 1809.5 12 1762.0 13 1877.5 14 1953.0 15 2082.0 16 2279.0 17 2492.5 18 2743.0 19 3066.0 20 3516.0 21 4149.0 22 4950.0 23 6151.5 24 7626.5 25 9438.0 26 11983.5 27 14953.0 28 17483.5 29 20080.5 30 22884.5 31 26801.5 32 32723.5 33 35820.5 34 36450.0 35 38768.0 36 42728.5 37 45982.5 38 47897.5 39 49564.0 40 51606.5 41 54258.5 42 56221.5 43 57829.0 44 59660.0 45 62919.0 46 65180.5 47 67112.0 48 70162.5 49 69380.0 50 65974.5 51 63364.0 52 61095.5 53 60164.5 54 60215.0 55 57273.0 56 52117.0 57 47104.0 58 43244.5 59 37606.0 60 30068.5 61 25221.5 62 21586.0 63 16427.0 64 11377.0 65 8349.5 66 6255.0 67 4863.0 68 3719.0 69 2675.5 70 2018.5 71 1456.5 72 1005.0 73 702.0 74 524.5 75 395.0 76 269.5 77 165.0 78 100.0 79 64.0 80 36.0 81 24.5 82 17.5 83 9.5 84 9.0 85 7.5 86 8.0 87 6.5 88 2.5 89 2.5 90 1.5 91 0.0 92 0.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4667981409717146 2 0.0412075409248252 3 0.054391747459006226 4 0.059963315910689416 5 0.08517328405345395 6 0.08324254251079147 7 0.04738591386134518 8 0.04021458813145592 9 0.04567582849498697 10-11 0.05091641268221373 12-13 0.04625505095778572 14-15 0.03971811173477127 16-17 0.04084897463833074 18-19 0.050668174483871405 20-21 0.057563679993380315 22-23 0.05033719021941498 24-25 0.06688640344223636 26-27 0.05888761705120602 28-29 0.04711009364096482 30-31 0.04840644867675249 32-33 0.047827226213953744 34-35 0.052736826136724084 36-37 0.04752382397153535 38-39 0.027499275971921498 40-41 0.024603163657927765 42-43 0.024327343437547406 44-45 0.015004619988691372 46-47 0.027609604060073647 48-49 0.027251037773579182 50-51 0.01599757278206065 52-53 0.01583208064983244 54-55 0.0161906469363269 56-57 0.014977037966653334 58-59 0.01450814359200673 60-61 0.017018107597467972 62-63 0.01563900649556619 64-65 0.01676986939912565 66-67 0.009157231316627823 68-69 0.012715312159534416 70-71 0.01977630980127153 72-73 0.01765249410434279 74-75 0.014149577305512268 76-77 0.009708871757388533 78-79 0.013653100908827626 80-81 0.017128435685620114 82-83 0.014287487415702446 84-85 0.012273999806925846 86-87 0.0038339010632869497 88-89 0.030891864682599884 90-91 0.034201707327164155 92-93 0.017928314324723145 94-95 0.034946421922191113 96-97 0.028657720897518994 98-99 0.025292714208878655 100-101 0.1486395167629739 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1812775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.29895028664593 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18240931754906 48.5379857233329 2 12.517017446657913 15.345600603995265 3 3.876000733162511 7.127843287593959 4 1.6081345948962416 3.943078503471209 5 0.8411806061711836 2.5781744079889037 6 0.47736359360975855 1.7557132316003545 7 0.30428599124201594 1.305668829504698 8 0.20912738169891198 1.0255431179470427 9 0.15756090097159822 0.8692486052199428 >10 0.7571339883923526 8.238713606931753 >50 0.040261397910431346 1.7067162635247188 >100 0.023565893590124006 2.8713642832694566 >500 0.0033401773253471006 1.415039454625468 >1k 0.002437426696874911 2.4054176611653766 >5k 1.8055012569443787E-4 0.8738924198289231 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8678 0.478713574492146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7114 0.39243700955717065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4550 0.25099640054612404 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2441 0.13465543158968984 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2365 0.13046296423990844 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2204 0.12158155314366097 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2131 0.11755457792610777 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2097 0.11567900042752134 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1982 0.10933513535877316 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1918 0.1058046365379046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.516404407607122E-5 5 0.0 0.0 0.0 0.0 5.516404407607122E-5 6 0.0 0.0 0.0 0.0 5.516404407607122E-5 7 0.0 0.0 0.0 0.0 5.516404407607122E-5 8 5.516404407607122E-5 0.0 0.0 0.0 5.516404407607122E-5 9 5.516404407607122E-5 0.0 0.0 0.0 1.1032808815214244E-4 10-11 5.516404407607122E-5 0.0 0.0 0.0 1.1032808815214244E-4 12-13 1.1032808815214244E-4 0.0 0.0 2.758202203803561E-5 1.1032808815214244E-4 14-15 1.6549213222821364E-4 0.0 0.0 5.516404407607122E-5 1.6549213222821364E-4 16-17 1.6549213222821364E-4 0.0 0.0 5.516404407607122E-5 1.6549213222821364E-4 18-19 1.6549213222821364E-4 0.0 0.0 5.516404407607122E-5 2.2065617630428487E-4 20-21 1.6549213222821364E-4 0.0 0.0 1.1032808815214244E-4 3.8614830853249854E-4 22-23 1.6549213222821364E-4 0.0 0.0 2.2065617630428487E-4 3.8614830853249854E-4 24-25 1.6549213222821364E-4 0.0 0.0 5.240584187226765E-4 3.8614830853249854E-4 26-27 1.6549213222821364E-4 0.0 0.0 0.001268773013749638 3.8614830853249854E-4 28-29 1.6549213222821364E-4 0.0 0.0 0.004826853856656231 3.8614830853249854E-4 30-31 1.6549213222821364E-4 0.0 0.0 0.01475638179034905 4.4131235260856975E-4 32-33 1.6549213222821364E-4 0.0 0.0 0.035498062362951825 5.516404407607122E-4 34-35 1.6549213222821364E-4 0.0 0.0 0.07005833597661044 5.792224627987478E-4 36-37 1.6549213222821364E-4 0.0 0.0 0.12684971935292577 6.068044848367834E-4 38-39 1.6549213222821364E-4 0.0 0.0 0.20832701245328294 6.068044848367834E-4 40-41 1.6549213222821364E-4 0.0 0.0 0.2939967729034215 6.068044848367834E-4 42-43 1.6549213222821364E-4 0.0 0.0 0.3763566907089959 6.068044848367834E-4 44-45 1.6549213222821364E-4 0.0 0.0 0.4795961991973632 6.34386506874819E-4 46-47 1.9307415426624924E-4 0.0 0.0 0.596350898484368 7.447145950269615E-4 48-49 3.309842644564273E-4 0.0 0.0 0.7105404697218353 9.377887492932106E-4 50-51 3.309842644564273E-4 0.0 0.0 0.8409758519397057 9.929527933692818E-4 52-53 3.309842644564273E-4 0.0 0.0 0.9648191308904857 0.001048116837445353 54-55 3.309842644564273E-4 0.0 0.0 1.0956130793948504 0.001048116837445353 56-57 3.309842644564273E-4 0.0 0.0 1.2474521107142365 0.001048116837445353 58-59 3.309842644564273E-4 0.0 0.0 1.4094964901876956 0.001048116837445353 60-61 3.309842644564273E-4 0.0 0.0 1.5867109817820744 0.001048116837445353 62-63 3.309842644564273E-4 0.0 0.0 1.752920246583277 0.001048116837445353 64-65 3.309842644564273E-4 5.516404407607122E-5 0.0 1.9197638978913543 0.001048116837445353 66-67 3.309842644564273E-4 5.516404407607122E-5 0.0 2.116423715022548 0.001048116837445353 68-69 3.309842644564273E-4 5.516404407607122E-5 0.0 2.333935540814497 0.001048116837445353 70-71 3.309842644564273E-4 5.516404407607122E-5 0.0 2.55166802278275 0.001048116837445353 72-73 3.309842644564273E-4 5.516404407607122E-5 0.0 2.775413385555295 0.0011860269476355312 74-75 3.309842644564273E-4 5.516404407607122E-5 0.0 2.9892292203941473 0.0012136089696735668 76-77 3.309842644564273E-4 5.516404407607122E-5 0.0 3.2220766504392437 0.0012136089696735668 78-79 3.309842644564273E-4 5.516404407607122E-5 0.0 3.485650453034712 0.0012411909917116022 80-81 3.309842644564273E-4 5.516404407607122E-5 0.0 3.76284977451697 0.001406683123939816 82-83 3.309842644564273E-4 5.516404407607122E-5 0.0 4.045924066693329 0.0015445932341299942 84-85 3.309842644564273E-4 1.1032808815214244E-4 0.0 4.331122174566618 0.0015721752561680298 86-87 3.309842644564273E-4 1.1032808815214244E-4 0.0 4.623105459861263 0.0016273393002441008 88-89 3.309842644564273E-4 1.1032808815214244E-4 0.0 4.9316379583787295 0.001682503344320172 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6330 0.0 34.89728 1 GTATCAA 9480 0.0 32.20965 1 TATCAAC 11275 0.0 26.766357 2 ATCAACG 11260 0.0 26.759808 3 CAACGCA 11410 0.0 26.49132 5 TCAACGC 11445 0.0 26.410307 4 TAAGGTG 1175 0.0 26.29103 5 AACGCAG 11745 0.0 25.812366 6 GAGTACT 7520 0.0 23.120836 12-13 ACGCAGA 13050 0.0 23.04207 7 CGCAGAG 13060 0.0 22.806187 8 TAGCCCT 1310 0.0 22.493267 4 GTACATG 6840 0.0 22.390472 1 GTAAGGT 1180 0.0 22.151993 4 TACATGG 6830 0.0 22.058231 2 CGAATAC 110 0.002868842 21.60277 3 GTAGCCC 975 0.0 21.447674 3 GTATAGC 925 0.0 21.147375 1 GTACTTT 8145 0.0 21.144001 14-15 GCAGAGT 13730 0.0 20.966715 9 >>END_MODULE