FastQCFastQC Report
Thu 26 May 2016
SRR938074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812775
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88940.49062900801257736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75650.4173159934354787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.273448166485085No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24920.1374687978375695No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22950.12660148115458345No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21910.12086442057067204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21700.11970597564507454No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21570.11898884307208561No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21540.11882335093985741No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18490.10199831749665568No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.10017790404214533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66550.035.4811481
GTATCAA98450.030.5958771
GTGTAGC12150.025.026091
ATCAACG119850.024.9297583
TATCAAC120550.024.7897852
TCAACGC121650.024.5978974
CAACGCA122050.024.4783655
AACGCAG125200.023.938376
TATACCG1000.001654546823.7486825
TAGCCCT14450.023.6665084
CCATGCG1050.002197415322.6177929
GAGTACT82050.022.2580612-13
TAGGACG3650.022.122064
ACGCAGA137750.021.6539737
CGCAGAG137350.021.6132898
GTATAGC8600.021.5454461
AGAGTAC112950.021.04686410-11
GTACTTT86500.021.03062614-15
AGTACTT85000.020.53562512-13
GTAAGGT10850.020.137134
Produced by FastQC (version 0.11.5)