FastQCFastQC Report
Thu 26 May 2016
SRR938073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860858
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92460.49686757399006265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76540.41131564041963437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.2510132422785618No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25130.13504523182316974No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23450.126017138330813No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22750.12225543270899766No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22750.12225543270899766No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21110.1134422938236018No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20520.11027171337092889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG702.048644E-433.9431465
GGTATCA68300.032.216661
GTATCAA102300.031.0274681
TATCAAC119950.026.2263492
ATCAACG119850.026.2085823
TCAACGC120350.026.0602154
CAACGCA121250.025.8667775
TAGGACG4800.025.7402174
AACGCAG125300.025.03076
GAGTACT77050.022.88810712-13
GTACATG69150.022.7093121
ACGCAGA137200.022.4871437
CGCAGAG137350.022.324148
AGGACGT6400.022.275195
TACATGG69000.021.9007072
GTACTTT81000.021.84705414-15
GGACGTG6400.021.5326846
TAGCCCT12700.021.3280544
TAAGGTG11600.021.302255
ACATGGG69300.021.120183
Produced by FastQC (version 0.11.5)