FastQCFastQC Report
Thu 26 May 2016
SRR938073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860858
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92500.4970825285970235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76580.4115305950265952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.26622128072104373No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24330.13074613968395224No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24030.12913398013174568No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23340.1254260131616706No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22970.1234376830472825No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22510.12096570506723242No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21450.11526940798276923No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.10473663224168638No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18740.10070623336116996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68800.036.0419241
GTATCAA102250.030.9411751
TCAACGC123200.025.3285644
TATCAAC123650.025.317292
ATCAACG123350.025.259263
CAACGCA124600.024.9677355
AACGCAG128100.024.2855576
GTGTAGC11050.022.78451
GAGTACT81550.022.56854812-13
CGCAGAG140200.022.0201998
ACGCAGA141150.021.9392937
TATTGCG1100.002879479221.5890335
GTACTTT85700.021.50338414-15
GTACATG69500.021.4620291
TACATGG68850.021.2506732
AGAGTAC114750.021.17136410-11
GTCCTAC5450.020.9189871
AGTACTT85000.020.87024712-13
GTGTAAG8450.020.8003641
ACATGGG68150.020.629173
Produced by FastQC (version 0.11.5)