##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1860858 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4844023563324 33.0 31.0 34.0 31.0 34.0 2 32.70485980123148 34.0 31.0 34.0 31.0 34.0 3 32.76374876535448 34.0 31.0 34.0 31.0 34.0 4 36.23336063256842 37.0 35.0 37.0 35.0 37.0 5 36.19692582668855 37.0 35.0 37.0 35.0 37.0 6 36.23313493023111 37.0 37.0 37.0 35.0 37.0 7 36.19397718686756 37.0 37.0 37.0 35.0 37.0 8 36.18823574931564 37.0 37.0 37.0 35.0 37.0 9 37.88807367354199 39.0 38.0 39.0 35.0 39.0 10-11 37.87561974100119 39.0 38.0 39.0 35.0 39.0 12-13 37.83076140146105 39.0 38.0 39.0 35.0 39.0 14-15 39.14309770009318 40.0 38.5 41.0 36.0 41.0 16-17 39.09969755886801 40.0 38.0 41.0 36.0 41.0 18-19 39.13677024254403 40.0 38.5 41.0 36.0 41.0 20-21 39.06579115655252 40.0 39.0 41.0 35.5 41.0 22-23 38.930627162308994 40.0 38.0 41.0 35.0 41.0 24-25 38.8944013997844 40.0 38.0 41.0 35.0 41.0 26-27 38.85636491338941 40.0 38.0 41.0 35.0 41.0 28-29 38.80188762388103 40.0 38.0 41.0 35.0 41.0 30-31 38.59797684723928 40.0 38.0 41.0 34.5 41.0 32-33 38.40963254584713 40.0 38.0 41.0 34.0 41.0 34-35 38.212310665295256 40.0 38.0 41.0 34.0 41.0 36-37 38.07908986069867 40.0 38.0 41.0 33.5 41.0 38-39 38.0512475965388 40.0 38.0 41.0 33.5 41.0 40-41 37.98355839080682 40.0 38.0 41.0 33.0 41.0 42-43 37.829249195801076 40.0 37.5 41.0 33.0 41.0 44-45 37.617065622417186 40.0 37.0 41.0 33.0 41.0 46-47 37.280428705468125 40.0 37.0 41.0 32.5 41.0 48-49 36.927444221966425 40.0 36.5 41.0 31.0 41.0 50-51 36.626228331232156 40.0 36.0 41.0 31.0 41.0 52-53 36.21569861859422 39.0 35.0 41.0 30.0 41.0 54-55 35.9993454632218 39.0 35.0 41.0 29.0 41.0 56-57 35.75826930372978 39.0 35.0 40.5 29.0 41.0 58-59 35.436749069515244 38.5 35.0 40.0 28.0 41.0 60-61 35.08393735577889 38.0 35.0 40.0 27.5 41.0 62-63 34.7362286644118 37.5 34.0 40.0 26.5 41.0 64-65 34.36070269735788 37.0 34.0 40.0 26.0 41.0 66-67 33.948726608908366 36.5 34.0 39.0 26.0 41.0 68-69 33.503761436928556 36.0 33.0 39.0 25.0 40.5 70-71 32.990486646482424 35.5 33.0 38.5 23.5 40.0 72-73 32.58410878207795 35.0 32.0 38.0 22.5 40.0 74-75 32.146660841396816 35.0 32.0 37.0 21.5 39.0 76-77 30.84534392199727 34.0 30.5 36.0 20.5 38.5 78-79 31.556339602484446 35.0 31.5 36.0 21.0 39.0 80-81 31.390389540738735 35.0 32.0 36.0 20.0 37.5 82-83 31.01439900304053 35.0 31.0 35.5 19.0 37.0 84-85 30.637372652830038 34.0 31.0 35.0 17.5 37.0 86-87 30.22509186622515 34.0 31.0 35.0 12.5 36.0 88-89 29.765071004880546 34.0 30.0 35.0 7.0 36.0 90-91 29.42872427665088 34.0 30.0 35.0 2.0 35.5 92-93 29.045590259976848 34.0 29.0 35.0 2.0 35.0 94-95 28.641827049672784 34.0 29.0 35.0 2.0 35.0 96-97 28.087481419861163 34.0 29.0 35.0 2.0 35.0 98-99 27.422641061273886 34.0 27.5 35.0 2.0 35.0 100-101 25.023513347068935 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15232383179280617 1101 2 0.23675319013557328 1101 3 0.23635548828750785 1101 4 0.07417388908218214 1101 5 0.1415412809076031 1101 6 0.16575862263597685 1101 7 0.17838850139177254 1101 8 0.15016770849876337 1101 9 0.10239109454468576 1101 10-11 0.1972271252919171 1101 12-13 0.22832151824248825 1101 14-15 0.2216922513764743 1101 16-17 0.23223549497895135 1101 18-19 0.2749243347709651 1101 20-21 0.27380509207015535 1101 22-23 0.33923397867078364 1101 24-25 0.3515454626697405 1101 26-27 0.3328176257779063 1101 28-29 0.464449737013247 1101 30-31 0.5017215694574233 1101 32-33 0.5082646482765227 1101 34-35 0.3422344228439229 1101 36-37 0.334096474929062 1101 38-39 0.38510314584762284 1101 40-41 0.40480065905776286 1101 42-43 0.4207406341344395 1101 44-45 0.5053913950420466 1101 46-47 0.3899276847120916 1101 48-49 0.3713446668962703 1101 50-51 0.4324999668789786 1101 52-53 0.4566005493527996 1101 54-55 0.5213806244002583 1101 56-57 0.6329202830243403 1101 58-59 0.5088045644104895 1101 60-61 0.5834077607386092 1101 62-63 0.5049107284967604 1101 64-65 0.4755708666797176 1101 66-67 0.4379789468259716 1101 68-69 0.5840124854260509 1101 70-71 0.5587474557240597 1101 72-73 0.5216354966228067 1101 74-75 0.4755134032463886 1101 76-77 0.3990635007560037 1101 78-79 0.45138382399548505 1101 80-81 0.687482935494188 1101 82-83 0.748890675260558 1101 84-85 0.7028850492042622 1101 86-87 0.7406757286073358 1101 88-89 0.6977265303903835 1101 90-91 0.6545966229495974 1101 92-93 0.8407621230751339 1101 94-95 0.7469186863691348 1101 96-97 0.9642720537579628 1101 98-99 1.0349995073593483 1101 100-101 1.2550840307432587 1102 1 0.1929670439914588 1102 2 0.16441827218388028 1102 3 0.1469204483810458 1102 4 0.06305510619606025 1102 5 0.1679685617395421 1102 6 0.08456544998664839 1102 7 0.06878992040560661 1102 8 0.10327993598541241 1102 9 0.13444125737149903 1102 10-11 0.16847780979943394 1102 12-13 0.1487212309477428 1102 14-15 0.2040203837754433 1102 16-17 0.15243613834987002 1102 18-19 0.17762969363808878 1102 20-21 0.04496894002085838 1102 22-23 0.1699384063431424 1102 24-25 0.05423673457067224 1102 26-27 0.016752730497636037 1102 28-29 0.09444895660854513 1102 30-31 0.11926209097939733 1102 32-33 0.17288176056749904 1102 34-35 0.19964224911261397 1102 36-37 0.23277183715588023 1102 38-39 0.13323116660903622 1102 40-41 0.2853868355773699 1102 42-43 0.16442200150928699 1102 44-45 0.41065621677908837 1102 46-47 0.2576335462127446 1102 48-49 0.21960257874493294 1102 50-51 0.19962522051050513 1102 52-53 0.2371090822184243 1102 54-55 0.29946262773879795 1102 56-57 0.35758755285124977 1102 58-59 0.3778212412621045 1102 60-61 0.30060100216935837 1102 62-63 0.3327312962610165 1102 64-65 0.47622726639507107 1102 66-67 0.4707906655945777 1102 68-69 0.4865068704803761 1102 70-71 0.5261278620106822 1102 72-73 0.4810402330179713 1102 74-75 0.42387640518550285 1102 76-77 0.3976610870568571 1102 78-79 0.39667051029131883 1102 80-81 0.40860862297781253 1102 82-83 0.42580114564618654 1102 84-85 0.39445919161886067 1102 86-87 0.585840460134893 1102 88-89 0.40336482207127133 1102 90-91 0.10813344350148668 1102 92-93 0.17718433789012167 1102 94-95 0.34634555186704574 1102 96-97 0.4195416372863967 1102 98-99 0.5555076169546211 1102 100-101 0.5725576226418632 1103 1 0.1831022405636702 1103 2 0.19164045630825655 1103 3 0.1505518084997064 1103 4 0.060483501286014985 1103 5 0.10219325778219002 1103 6 0.012410660959844222 1103 7 0.027301361190119167 1103 8 0.11911149776869934 1103 9 0.1255574735945757 1103 10-11 0.11648853053456065 1103 12-13 0.11659712338988726 1103 14-15 0.23885388346938896 1103 16-17 0.20765770274920925 1103 18-19 0.12430254485581571 1103 20-21 0.19040113473644027 1103 22-23 0.12552297976150584 1103 24-25 0.231800656414201 1103 26-27 0.18943980999261356 1103 28-29 0.24899447465041646 1103 30-31 0.1718298226079824 1103 32-33 0.24980513055096054 1103 34-35 0.16265145863301456 1103 36-37 0.2582046922341945 1103 38-39 0.24223292672972718 1103 40-41 0.24278962891177258 1103 42-43 0.27070389258499006 1103 44-45 0.2618710937357349 1103 46-47 0.31576864373055 1103 48-49 0.3398352677166159 1103 50-51 0.3983239249296062 1103 52-53 0.2182131833250338 1103 54-55 0.27198423556968265 1103 56-57 0.38986405181118045 1103 58-59 0.39048909404184684 1103 60-61 0.21156727795208496 1103 62-63 0.34407683614566054 1103 64-65 0.23335188192425704 1103 66-67 0.2653794373960707 1103 68-69 0.29692720406514894 1103 70-71 0.4243764403259007 1103 72-73 0.18146635747603312 1103 74-75 0.30389516202723854 1103 76-77 0.3711770487862971 1103 78-79 0.26888777979297274 1103 80-81 0.23888269891444835 1103 82-83 0.27818149934662983 1103 84-85 0.3632100646945986 1103 86-87 0.5526550099579914 1103 88-89 0.3150927373822867 1103 90-91 0.32651698980180655 1103 92-93 0.5337186272255181 1103 94-95 0.4241102493828137 1103 96-97 0.5781004463983912 1103 98-99 0.448292011059106 1103 100-101 0.580719134669355 1104 1 0.06579337030944998 1104 2 0.029041218331734342 1104 3 0.026677918490435104 1104 4 -0.0251289174758611 1104 5 0.03740842613302675 1104 6 -0.02296396874474027 1104 7 0.03361235321764866 1104 8 0.02230185916268823 1104 9 -0.02004339899514207 1104 10-11 0.033963100896365006 1104 12-13 0.06716440223054576 1104 14-15 0.08205131346102235 1104 16-17 0.06981109370593686 1104 18-19 0.12577611462896954 1104 20-21 0.015285583463430896 1104 22-23 0.08882015220304851 1104 24-25 0.0921743607533827 1104 26-27 0.04501997230767785 1104 28-29 0.002196089681696378 1104 30-31 -0.1201640055033053 1104 32-33 -0.14535544721137228 1104 34-35 0.07022342519567815 1104 36-37 0.09376636515155923 1104 38-39 0.05501777509517325 1104 40-41 0.13981616122732277 1104 42-43 0.25943816585076007 1104 44-45 0.030145120102083922 1104 46-47 -0.04340163783128048 1104 48-49 -0.10423848330874108 1104 50-51 -0.006280454496483401 1104 52-53 -0.09665403569522368 1104 54-55 -0.056263916386065205 1104 56-57 -0.051877330648913755 1104 58-59 0.053880713455178864 1104 60-61 -0.06620278844735594 1104 62-63 -0.11025817239755753 1104 64-65 -0.18966264213344886 1104 66-67 -0.1199040587418736 1104 68-69 -0.01567212346285629 1104 70-71 0.09131817932031794 1104 72-73 0.006676027365351445 1104 74-75 -0.05290319906108465 1104 76-77 0.02439711142458023 1104 78-79 0.04549949766518324 1104 80-81 0.12144636703901313 1104 82-83 0.1305627331751218 1104 84-85 0.04254796873600242 1104 86-87 0.03437919256183619 1104 88-89 0.15055202309572024 1104 90-91 0.1866341291212379 1104 92-93 0.35151865755362266 1104 94-95 0.1286177937973676 1104 96-97 0.352632181655526 1104 98-99 0.3229599242825181 1104 100-101 0.4274477291766239 1105 1 -0.029092997658914044 1105 2 0.05887716271259791 1105 3 0.019827042583031584 1105 4 -0.0014251861614482664 1105 5 -0.031885355612033095 1105 6 -0.034053119929097875 1105 7 0.01216457374463431 1105 8 -0.10039633717713059 1105 9 -0.07196926009865479 1105 10-11 -0.06808688132767315 1105 12-13 -0.004649782338447039 1105 14-15 0.05261070423824776 1105 16-17 0.00996441032789619 1105 18-19 -0.06223116744464363 1105 20-21 0.002646718459374142 1105 22-23 -0.041272505886531974 1105 24-25 -0.03178594307862426 1105 26-27 -0.12162198591160944 1105 28-29 -0.14397555524295313 1105 30-31 0.02863393559119487 1105 32-33 0.1606078447896735 1105 34-35 0.16652692851466355 1105 36-37 0.22690705731000094 1105 38-39 -0.09148623374451859 1105 40-41 -0.096618689374111 1105 42-43 0.0446765769535844 1105 44-45 -0.011045555974689591 1105 46-47 -0.018603816466864487 1105 48-49 0.06194714518520783 1105 50-51 0.0894263751160409 1105 52-53 0.11383638039220756 1105 54-55 0.18296411829839343 1105 56-57 0.23064611625574116 1105 58-59 0.2821600821275254 1105 60-61 0.17713743783683356 1105 62-63 0.25277852684755686 1105 64-65 0.26834796357931623 1105 66-67 0.2406091314744856 1105 68-69 0.12304759492297279 1105 70-71 0.10152530952875338 1105 72-73 0.02714591708662084 1105 74-75 0.07096307147974557 1105 76-77 0.1490070356360036 1105 78-79 0.19856889395168764 1105 80-81 0.17847304001974607 1105 82-83 0.20776378284644537 1105 84-85 0.3454417656167479 1105 86-87 0.37359144207818673 1105 88-89 0.22817001016769822 1105 90-91 0.07499049213944176 1105 92-93 0.10199171842761245 1105 94-95 0.02811498395272949 1105 96-97 0.27117224280258156 1105 98-99 0.42427034737867686 1105 100-101 0.6292901886808906 1106 1 -0.11673891881105192 1106 2 2.2709843593560208E-4 1106 3 0.07314359271691728 1106 4 -0.01218435071244528 1106 5 -0.0292311750041776 1106 6 0.016869527711747878 1106 7 0.03907683869463341 1106 8 -0.024831749655056967 1106 9 0.07187130752635795 1106 10-11 0.053382618287649564 1106 12-13 -0.08843965041134538 1106 14-15 -0.17254449205574218 1106 16-17 -0.10335663452470101 1106 18-19 -0.1200562430537957 1106 20-21 0.009693912458978104 1106 22-23 0.06168471281214494 1106 24-25 0.11987581785592738 1106 26-27 0.015114246825142175 1106 28-29 0.07058807154666624 1106 30-31 0.03713753874799153 1106 32-33 0.0143869080399881 1106 34-35 -0.036719248103729285 1106 36-37 -0.07547391329770647 1106 38-39 -7.994999653959667E-5 1106 40-41 -0.09052019816697765 1106 42-43 -0.10841972102767272 1106 44-45 0.01146850095732077 1106 46-47 -0.10095996769719306 1106 48-49 -0.04889045822202576 1106 50-51 -0.021934381833951022 1106 52-53 -0.08526489458779452 1106 54-55 -0.403660375293903 1106 56-57 -0.21986372263976506 1106 58-59 -0.17053478060584837 1106 60-61 -0.1501387790316997 1106 62-63 -0.11196338498269398 1106 64-65 -0.16389100781907473 1106 66-67 -0.08802274614230754 1106 68-69 -0.2994797493161627 1106 70-71 -0.26615976816918874 1106 72-73 -0.19106953195054643 1106 74-75 -0.36242883354280053 1106 76-77 -0.17130154703482958 1106 78-79 -0.10989566748789414 1106 80-81 -0.4156418312109338 1106 82-83 -0.4719965316307615 1106 84-85 -0.25935790498926536 1106 86-87 -0.1568247349721723 1106 88-89 -0.17474967052136137 1106 90-91 -0.3139046546032809 1106 92-93 -0.11081940550905856 1106 94-95 -0.0955708439585976 1106 96-97 -0.14214352540335184 1106 98-99 -0.04264343768300094 1106 100-101 -0.005959697447433854 1107 1 0.03861337066808801 1107 2 0.08920411683615725 1107 3 0.059896106740417565 1107 4 0.06821494682356644 1107 5 0.033695961718507306 1107 6 -0.028447080889748122 1107 7 0.02717830244186814 1107 8 0.041745022104123564 1107 9 0.05863644822434111 1107 10-11 0.05879998775151307 1107 12-13 0.06155182658337566 1107 14-15 0.1593176583300533 1107 16-17 0.0782273081333642 1107 18-19 0.010324693193069834 1107 20-21 0.07797186554878266 1107 22-23 -0.0058906184539182505 1107 24-25 0.08701253001719778 1107 26-27 0.006692254946194964 1107 28-29 0.026099562346551863 1107 30-31 0.10833961920305768 1107 32-33 0.09552749659032855 1107 34-35 0.22026225066149152 1107 36-37 0.19984519959422897 1107 38-39 0.10965558430645217 1107 40-41 0.08005170600919342 1107 42-43 0.09401138731024616 1107 44-45 0.1769026638982396 1107 46-47 0.23988474630336754 1107 48-49 0.23748199289958194 1107 50-51 0.3481758069453562 1107 52-53 0.3106431684908699 1107 54-55 0.2593363133607127 1107 56-57 0.27326420977385624 1107 58-59 0.29206027457798456 1107 60-61 0.2076995974304907 1107 62-63 0.2850634540661403 1107 64-65 0.3821413753920808 1107 66-67 0.23869666494959318 1107 68-69 0.45170742114078877 1107 70-71 0.39475536058606053 1107 72-73 0.3641887414148499 1107 74-75 0.31454505765104557 1107 76-77 0.1510761294255758 1107 78-79 0.3452009844441477 1107 80-81 0.2458785887522552 1107 82-83 0.22418332061610258 1107 84-85 0.3888124372672692 1107 86-87 0.5233773759973559 1107 88-89 0.5293581549653688 1107 90-91 0.4457102949584133 1107 92-93 0.5697983334954735 1107 94-95 0.5401227655762213 1107 96-97 0.7467491047224186 1107 98-99 0.7196538910387922 1107 100-101 0.6408856331557331 1108 1 0.07186585640734933 1108 2 -0.01430121002591278 1108 3 0.033611732461885424 1108 4 0.03810877274788993 1108 5 -0.004428131988611028 1108 6 0.05332459685779156 1108 7 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2316 52-53 -0.11912206729380159 2316 54-55 -0.1549973351824221 2316 56-57 -0.3245325495158724 2316 58-59 -0.1889247378828287 2316 60-61 -0.11192171517128457 2316 62-63 -0.21133550683064328 2316 64-65 -0.2277782153308081 2316 66-67 -0.21160156108376071 2316 68-69 -0.2658124349455022 2316 70-71 -0.4704330242249597 2316 72-73 -0.2850566254983491 2316 74-75 -0.15072247686064344 2316 76-77 0.03747949883993584 2316 78-79 -0.13570636991969565 2316 80-81 -0.24016355212994966 2316 82-83 -0.3436752952419653 2316 84-85 -0.2578512714466825 2316 86-87 -0.34919184270062686 2316 88-89 -0.4290838720814314 2316 90-91 -0.4408066020702641 2316 92-93 -0.6301642817072342 2316 94-95 -0.5954885149252931 2316 96-97 -0.4659626970827553 2316 98-99 -0.36492820659164593 2316 100-101 -0.3802733571185364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 11.0 5 33.0 6 62.0 7 142.0 8 375.0 9 733.0 10 1249.0 11 2148.0 12 3305.0 13 4854.0 14 6734.0 15 8929.0 16 11853.0 17 17220.0 18 16938.0 19 11871.0 20 9654.0 21 9190.0 22 9446.0 23 10081.0 24 11877.0 25 13757.0 26 16211.0 27 19659.0 28 23911.0 29 29217.0 30 36060.0 31 44542.0 32 56552.0 33 73816.0 34 103425.0 35 155752.0 36 259529.0 37 431318.0 38 407203.0 39 53183.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01408275107504 19.002847073769196 15.057516478957556 22.925553696198207 2 15.506903327614602 20.195834354535645 40.24391685908268 24.053345458767076 3 19.26976324997756 24.010030423396294 30.36116295729815 26.359043369327996 4 12.745733419745086 17.012098720052794 37.129431692262386 33.11273616793974 5 13.275227489269728 37.666945928676945 34.516085867962985 14.541740714090338 6 31.96047199732596 36.544808899980545 17.06083967718117 14.433879425512316 7 28.19586448831668 33.35133578166631 20.28918918047481 18.1636105495422 8 26.430731348970284 34.94286747608215 19.482343811883716 19.144057363063848 9 26.199368676962298 16.826252892489148 20.3172844296221 36.657094000926456 10-11 24.95792263568741 26.614792746141834 27.087343580219446 21.33994103795131 12-13 26.170777136138273 23.969185182319126 27.533051957752818 22.32698572378978 14-15 22.48484301327667 25.590695259928488 26.33639966080163 25.58806206599321 16-17 21.655212699327112 28.629642994656486 27.389567534699783 22.32557677131662 18-19 21.63483188937576 26.865429817858217 29.99780208914383 21.5019362036222 20-21 22.34525686538145 25.929087549936643 30.331411639147106 21.394243945534804 22-23 21.861797085000575 26.13799655857674 30.21117140587836 21.78903495054432 24-25 21.916604550103212 26.216459071051496 30.272325821483715 21.59461055736158 26-27 21.372588383930005 26.498798542394482 30.195514683690515 21.933098389984995 28-29 21.374775170794962 27.082790348747913 29.926641327087466 21.61579315336966 30-31 21.702811283827135 26.459702997219566 29.81619231558776 22.021293403365544 32-33 21.26128014251571 26.584956585641756 29.852526872836165 22.301236399006367 34-35 22.05241987824218 26.771429024456644 29.617631637576313 21.558519459724863 36-37 22.164444561480632 26.245517319952516 29.73471240178141 21.855325716785444 38-39 21.65981506728381 26.68364046611838 29.60613851706074 22.050405949537076 40-41 22.215957707538774 26.41072511190131 29.634283724110055 21.739033456449857 42-43 21.807209360818447 26.57363497021538 29.37047077108946 22.248684897876704 44-45 21.853961552651015 26.70957642245599 29.569226643637204 21.867235381255796 46-47 22.345886868254787 26.972384229416875 28.808930508899934 21.872798393428404 48-49 21.979296647030562 27.04362718702878 28.74733053247481 22.229745633465853 50-51 22.017101788529807 27.391961127608877 28.733492829651695 21.857444254209618 52-53 22.530520867255856 27.057464889851886 28.51751718830776 21.894497054584498 54-55 21.88012196524399 27.177678253794756 28.742574661795793 22.19962511916546 56-57 22.131887548646915 27.301518976730087 28.359068773651725 22.207524700971273 58-59 22.183476654317523 27.087155494938358 28.303046229212548 22.42632162153157 60-61 21.753594309721645 27.308666217411538 28.7832010825114 22.154538390355416 62-63 21.84043597093384 27.23012717789321 28.880306826205977 22.049130024966978 64-65 21.495433826761634 27.37197034916152 28.591273487821212 22.541322336255643 66-67 21.528241273649037 27.40136539166342 28.43430288608803 22.636090448599518 68-69 22.131129040752256 27.01660097998016 28.577873385015696 22.274396594251886 70-71 21.90419688122361 27.1333169967832 28.174046595710152 22.788439526283035 72-73 21.864779580172158 27.270672990631205 28.317286971923707 22.547260457272937 74-75 21.83809833958314 27.80150339252108 27.980533710793626 22.379864557102152 76-77 21.866445478376104 27.14360794859146 28.357134182189075 22.632812390843362 78-79 21.881814732773808 27.475470992413175 28.186755786846714 22.455958487966303 80-81 22.262096301813465 27.497208277042095 27.867655672813296 22.37303974833115 82-83 22.10399718839374 27.536840532700506 28.073609055607683 22.28555322329807 84-85 21.995836219622756 27.70606762972449 27.933549332575243 22.364546818077514 86-87 22.074771724004698 27.75282055167785 27.61471166007156 22.557696064245892 88-89 21.9786963691182 27.80324544101063 27.438735872432936 22.779322317438233 90-91 21.927223180116005 27.72972187224841 27.63275024068257 22.710304706953018 92-93 22.158207425674604 27.454000299324854 27.892992171069796 22.49480010393075 94-95 21.7644226480473 27.295822679645624 28.058320409187587 22.881434263119484 96-97 21.969441507477065 26.91167915866744 28.141703488848556 22.97717584500694 98-99 21.736129247906074 27.217256770801423 27.762784693942255 23.283829287350244 100-101 21.7693666040074 26.97236973482125 28.09316992484112 23.16509373633023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 776.0 1 515.0 2 364.0 3 503.5 4 418.5 5 383.0 6 767.5 7 1727.0 8 2864.0 9 3251.0 10 2627.5 11 1944.5 12 1803.5 13 1914.0 14 2054.0 15 2209.0 16 2358.5 17 2479.0 18 2758.5 19 3125.0 20 3579.5 21 4241.5 22 5094.0 23 6115.5 24 7527.0 25 9552.0 26 12240.0 27 15233.0 28 17941.0 29 20637.5 30 23595.5 31 27913.5 32 34539.5 33 37650.0 34 37847.5 35 40156.5 36 43934.5 37 47406.5 38 49440.5 39 51029.0 40 53356.0 41 56537.0 42 58647.5 43 60159.5 44 62110.0 45 65826.0 46 67593.5 47 69780.5 48 74011.0 49 72550.5 50 68143.5 51 65143.5 52 62882.0 53 62165.5 54 62612.5 55 59211.5 56 53421.0 57 47752.5 58 43606.0 59 38092.0 60 29746.0 61 24649.5 62 21094.0 63 15346.0 64 9840.5 65 7000.5 66 5254.0 67 4128.0 68 3107.0 69 2187.5 70 1561.5 71 994.5 72 669.0 73 498.5 74 353.5 75 257.0 76 191.0 77 114.0 78 60.0 79 30.0 80 15.5 81 12.0 82 5.5 83 3.0 84 2.5 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01644402743250694 3 0.006394899557086033 4 0.0 5 5.373865174021876E-5 6 0.0 7 0.0 8 4.8364786566196884E-4 9 2.1495460696087504E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8808528109076565E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005857513039683845 26-27 0.02200597788761958 28-29 5.373865174021876E-5 30-31 0.0 32-33 5.64255843272297E-4 34-35 0.019426522604089082 36-37 0.004567785397918594 38-39 0.018459226872765144 40-41 0.01558420900466344 42-43 0.008920616188876313 44-45 0.0028750178681017035 46-47 5.373865174021876E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.1495460696087504E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002633193935270719 86-87 4.030398880516407E-4 88-89 8.060797761032814E-5 90-91 2.4182393283098442E-4 92-93 1.8808528109076565E-4 94-95 0.0 96-97 2.686932587010938E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1860858.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.93753786942136 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47804003166074 45.663649184145484 2 13.295487290184452 15.67206571315313 3 4.292288821882704 7.589308049586177 4 1.826353862287478 4.305631996861288 5 0.9433958060917425 2.7800713023692682 6 0.5217743930494814 1.8451258829788888 7 0.3347366434586557 1.3809987520089262 8 0.2257452993606092 1.0643897703927814 9 0.17412471683110564 0.9236233883012004 >10 0.8255015313225608 8.661806215202922 >50 0.04917320516024975 1.9743649468983624 >100 0.026448594910855702 3.025207912378465 >500 0.004011991673356979 1.5817892553819008 >1k 0.0027354488681979386 2.623328208923947 >5k 1.8236325787986257E-4 0.9086394214173124 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9250 0.4970825285970235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7658 0.4115305950265952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4954 0.26622128072104373 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2433 0.13074613968395224 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2403 0.12913398013174568 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2334 0.1254260131616706 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2297 0.1234376830472825 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2251 0.12096570506723242 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2145 0.11526940798276923 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 0.10473663224168638 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1874 0.10070623336116996 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.686932587010938E-5 14-15 0.0 0.0 0.0 0.0 1.343466293505469E-4 16-17 0.0 0.0 0.0 0.0 2.686932587010938E-4 18-19 0.0 0.0 0.0 0.0 2.686932587010938E-4 20-21 0.0 0.0 0.0 2.686932587010938E-5 2.686932587010938E-4 22-23 0.0 0.0 0.0 1.6121595522065627E-4 2.9556258457120316E-4 24-25 0.0 0.0 0.0 4.5677853979185945E-4 3.761705621815313E-4 26-27 0.0 0.0 0.0 0.0012897276417652504 3.761705621815313E-4 28-29 0.0 0.0 0.0 0.005105171915320782 3.761705621815313E-4 30-31 0.0 0.0 0.0 0.015933510240974863 4.2990921392175007E-4 32-33 0.0 0.0 0.0 0.038288789364905866 4.8364786566196884E-4 34-35 2.686932587010938E-5 0.0 0.0 0.0704513724314268 4.8364786566196884E-4 36-37 5.373865174021876E-5 0.0 0.0 0.12247038731595855 4.8364786566196884E-4 38-39 5.373865174021876E-5 0.0 0.0 0.20302462627454648 4.8364786566196884E-4 40-41 5.373865174021876E-5 0.0 0.0 0.29005437276783075 5.373865174021875E-4 42-43 5.373865174021876E-5 0.0 0.0 0.3753913517312981 5.373865174021875E-4 44-45 5.373865174021876E-5 0.0 0.0 0.4837822122913194 5.373865174021875E-4 46-47 5.373865174021876E-5 0.0 0.0 0.6038343602789681 5.373865174021875E-4 48-49 5.373865174021876E-5 0.0 0.0 0.729824629283911 5.373865174021875E-4 50-51 5.373865174021876E-5 0.0 0.0 0.8636070027911855 5.373865174021875E-4 52-53 5.373865174021876E-5 0.0 0.0 0.9872327711195588 5.373865174021875E-4 54-55 5.373865174021876E-5 0.0 0.0 1.1172803083308882 5.911251691424063E-4 56-57 5.373865174021876E-5 0.0 0.0 1.269575647362668 5.911251691424063E-4 58-59 5.373865174021876E-5 0.0 0.0 1.4279971926928332 5.911251691424063E-4 60-61 5.373865174021876E-5 0.0 0.0 1.6034270212987773 5.911251691424063E-4 62-63 5.373865174021876E-5 0.0 0.0 1.772435081021765 5.911251691424063E-4 64-65 5.373865174021876E-5 0.0 0.0 1.9427866070382587 6.179944950125158E-4 66-67 5.373865174021876E-5 0.0 0.0 2.140168674880082 6.448638208826251E-4 68-69 5.373865174021876E-5 0.0 0.0 2.351630269477843 6.448638208826251E-4 70-71 5.373865174021876E-5 0.0 0.0 2.5681701666650545 6.448638208826251E-4 72-73 8.060797761032814E-5 0.0 0.0 2.7963176126281533 8.060797761032814E-4 74-75 1.0747730348043752E-4 0.0 0.0 3.020998915554008 8.329491019733908E-4 76-77 1.0747730348043752E-4 0.0 0.0 3.2637632747904464 8.598184278435001E-4 78-79 1.0747730348043752E-4 0.0 0.0 3.532698357424371 8.598184278435001E-4 80-81 1.0747730348043752E-4 0.0 0.0 3.8118169145630674 8.866877537136095E-4 82-83 1.0747730348043752E-4 0.0 0.0 4.101038338228925 9.135570795837189E-4 84-85 1.0747730348043752E-4 0.0 0.0 4.394343899427039 9.135570795837189E-4 86-87 1.0747730348043752E-4 0.0 0.0 4.696650684791639 9.672957313239377E-4 88-89 1.0747730348043752E-4 0.0 0.0 5.012822042305217 0.0010210343830641564 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6880 0.0 36.041924 1 GTATCAA 10225 0.0 30.941175 1 TCAACGC 12320 0.0 25.328564 4 TATCAAC 12365 0.0 25.31729 2 ATCAACG 12335 0.0 25.25926 3 CAACGCA 12460 0.0 24.967735 5 AACGCAG 12810 0.0 24.285557 6 GTGTAGC 1105 0.0 22.7845 1 GAGTACT 8155 0.0 22.568548 12-13 CGCAGAG 14020 0.0 22.020199 8 ACGCAGA 14115 0.0 21.939293 7 TATTGCG 110 0.0028794792 21.589033 5 GTACTTT 8570 0.0 21.503384 14-15 GTACATG 6950 0.0 21.462029 1 TACATGG 6885 0.0 21.250673 2 AGAGTAC 11475 0.0 21.171364 10-11 GTCCTAC 545 0.0 20.918987 1 AGTACTT 8500 0.0 20.870247 12-13 GTGTAAG 845 0.0 20.800364 1 ACATGGG 6815 0.0 20.62917 3 >>END_MODULE