FastQCFastQC Report
Thu 26 May 2016
SRR938072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787088
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.47194094526962305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70600.39505609125012314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.2393278898409032No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23840.13340137698871013No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23050.12898077766735605No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21350.11946809558342959No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20930.11711790353916539No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20650.11555110884298926No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19370.10838861880332698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71800.032.9545061
GTATCAA102550.029.2010081
ATCAACG120700.024.624533
CAACGCA121650.024.5495035
TATCAAC121450.024.511622
TCAACGC122050.024.3911194
GAGTACT76150.024.28209112-13
AACGCAG126100.023.7922976
TAGACTG5500.023.3451295
GTACATG65650.023.2058831
TGTAAGG8250.023.0569172
GTACTTT81500.022.48366414-15
TACATGG65550.022.4171812
GTGTAAG10000.021.8998281
ACGCAGA137500.021.745667
ACATGGG65400.021.5233143
CGCAGAG138800.021.4031988
AGAGTAC106900.021.0312710-11
TAGCGTG1150.00369923620.6760395
TAAGGTG12500.020.5437135
Produced by FastQC (version 0.11.5)