FastQCFastQC Report
Thu 26 May 2016
SRR938072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787088
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86250.4826287233756815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.4121229620477559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.2574019858003635No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24190.1353598703589303No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22960.1284771650864423No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22230.1243923074856974No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21880.12243381411547725No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21180.11851682737503692No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21120.11818108565442777No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19940.11157816514911409No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.1019535691583179No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.033.0621641
GTATCAA100750.029.7582021
TATCAAC120500.024.759062
ATCAACG120850.024.6439043
TCAACGC121400.024.5281374
CAACGCA121900.024.4664885
AACGCAG125900.023.7639356
GAGTACT78900.023.08621212-13
GTACATG68550.021.9722291
GTACTTT82400.021.9032514-15
TACATGG67250.021.758052
TAGCCCT14600.021.4687524
CGCAGAG139850.021.2916168
ACGCAGA140350.021.2834387
ACATGGG66400.021.0313853
ACCGCTA1604.9992537E-520.776948
AGAGTAC113400.020.56461510-11
GTGTAGC12500.020.5260831
TAAGGTG10650.020.0668225
AGTACTT82550.020.02287712-13
Produced by FastQC (version 0.11.5)