##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938072_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787088 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49845446894613 34.0 31.0 34.0 31.0 34.0 2 32.71479244446832 34.0 31.0 34.0 31.0 34.0 3 32.76893471390329 34.0 31.0 34.0 31.0 34.0 4 36.23391797158282 37.0 35.0 37.0 35.0 37.0 5 36.20824044479063 37.0 35.0 37.0 35.0 37.0 6 36.23791273848853 37.0 37.0 37.0 35.0 37.0 7 36.196384285496855 37.0 37.0 37.0 35.0 37.0 8 36.1805014638339 37.0 37.0 37.0 35.0 37.0 9 37.86085576088027 39.0 38.0 39.0 35.0 39.0 10-11 37.84205898086719 39.0 38.0 39.0 35.0 39.0 12-13 37.766747636378284 39.0 38.0 39.0 35.0 39.0 14-15 39.036126928276616 40.0 38.0 41.0 35.5 41.0 16-17 38.926825371777994 40.0 38.0 41.0 35.5 41.0 18-19 39.182241725085724 40.0 39.0 41.0 36.0 41.0 20-21 39.16942058812997 40.0 39.0 41.0 36.0 41.0 22-23 39.078857336628076 40.0 39.0 41.0 35.5 41.0 24-25 38.979868926432275 40.0 38.5 41.0 35.0 41.0 26-27 38.81173338973794 40.0 38.0 41.0 35.0 41.0 28-29 38.697455861155134 40.0 38.0 41.0 34.5 41.0 30-31 38.44662294190326 40.0 38.0 41.0 34.0 41.0 32-33 38.22708730627703 40.0 38.0 41.0 34.0 41.0 34-35 38.0669441012418 40.0 38.0 41.0 34.0 41.0 36-37 38.053528421655784 40.0 38.0 41.0 33.5 41.0 38-39 37.90305653666748 40.0 37.0 41.0 33.0 41.0 40-41 37.72480006580538 40.0 37.0 41.0 33.0 41.0 42-43 37.492463157941856 40.0 37.0 41.0 33.0 41.0 44-45 37.2116764815163 40.0 37.0 41.0 32.0 41.0 46-47 36.805825454594284 40.0 36.0 41.0 31.0 41.0 48-49 36.391450784740314 39.0 36.0 41.0 30.0 41.0 50-51 36.00508732642153 39.0 35.0 41.0 29.0 41.0 52-53 35.48615205294871 39.0 35.0 40.0 27.5 41.0 54-55 35.181386702837244 38.0 34.5 40.0 27.0 41.0 56-57 34.8486943564055 38.0 34.0 40.0 26.0 41.0 58-59 34.411893258753906 38.0 34.0 40.0 25.5 41.0 60-61 33.94122869159213 37.0 33.0 40.0 23.5 41.0 62-63 33.48548980240481 36.5 33.0 39.5 22.5 41.0 64-65 33.01338798089405 36.0 32.0 39.0 21.5 40.5 66-67 32.46759225063343 35.5 31.5 39.0 19.5 40.0 68-69 31.899873984940868 35.0 31.0 38.0 17.5 40.0 70-71 31.29500981484963 35.0 30.5 37.5 12.0 40.0 72-73 30.71271000644624 34.5 30.0 37.0 8.0 39.0 74-75 30.132145702953633 34.0 29.0 36.0 7.0 39.0 76-77 28.795761036949497 32.5 28.0 35.0 4.5 37.0 78-79 29.468066765598557 34.0 29.0 35.0 2.0 37.5 80-81 29.144199670077803 34.0 29.0 35.0 2.0 37.0 82-83 28.586991239379373 34.0 28.5 35.0 2.0 36.5 84-85 27.99031608963856 33.0 26.5 35.0 2.0 36.0 86-87 27.35970920290439 33.0 26.0 35.0 2.0 36.0 88-89 26.617491695988107 32.0 25.0 35.0 2.0 35.0 90-91 26.081272438738328 32.0 24.0 34.5 2.0 35.0 92-93 25.271127387123634 31.5 20.5 34.0 2.0 35.0 94-95 24.45315843427968 31.0 15.5 34.0 2.0 35.0 96-97 23.406677231339476 31.0 2.0 34.0 2.0 35.0 98-99 21.97362441021371 30.0 2.0 34.0 2.0 35.0 100-101 17.624581721773076 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10927566396195232 1101 2 0.09600167685191252 1101 3 0.18043375643839 1101 4 0.10933677441349232 1101 5 0.04509566528105324 1101 6 0.01274951217173026 1101 7 0.006814602892319499 1101 8 0.06199482460777972 1101 9 -0.07339992519294469 1101 10-11 0.07778678573146891 1101 12-13 0.10038737830110733 1101 14-15 0.09445946939860761 1101 16-17 0.18265666656769497 1101 18-19 0.19634824835461018 1101 20-21 0.19717823768253595 1101 22-23 0.21158030822697782 1101 24-25 0.174230956762365 1101 26-27 0.18479897157807557 1101 28-29 0.20531388912493753 1101 30-31 0.21703082257264583 1101 32-33 0.2370353142511803 1101 34-35 0.20885311976849863 1101 36-37 0.3546866473610848 1101 38-39 0.2921226355364652 1101 40-41 0.31712765557864486 1101 42-43 0.2719152089241348 1101 44-45 0.2721990623444128 1101 46-47 0.2853381193977711 1101 48-49 0.46573508871632896 1101 50-51 0.2147816839764829 1101 52-53 0.2786516023149872 1101 54-55 0.2617772933144664 1101 56-57 0.47284578172240543 1101 58-59 0.41316507552543413 1101 60-61 0.16053162219516537 1101 62-63 0.15627398590907404 1101 64-65 0.28216666533927537 1101 66-67 0.09098611853010397 1101 68-69 0.1690350397328757 1101 70-71 0.08053326316280973 1101 72-73 0.19514809119465326 1101 74-75 0.17452296956705027 1101 76-77 -0.03558383622355876 1101 78-79 0.2330646472906217 1101 80-81 0.2086671530418691 1101 82-83 -0.01431542511393502 1101 84-85 0.019057918939466845 1101 86-87 0.05975108534255824 1101 88-89 -0.07150752806903782 1101 90-91 -0.1396251816602856 1101 92-93 -0.27647847454126406 1101 94-95 -0.40871573820051665 1101 96-97 -0.3009594047808335 1101 98-99 -0.20358227706624987 1101 100-101 -0.6984015675113291 1102 1 0.1497041974119213 1102 2 0.18679168088254272 1102 3 0.09622354607365935 1102 4 0.0761290767624061 1102 5 0.08417422260036744 1102 6 0.06605320177298069 1102 7 0.08723644720698331 1102 8 0.03517825270385799 1102 9 0.06486793179677619 1102 10-11 0.10267308472437975 1102 12-13 0.10128926812475925 1102 14-15 0.04969693370178163 1102 16-17 0.03848078804696797 1102 18-19 0.1521631508333101 1102 20-21 0.14187566865437162 1102 22-23 0.16360684465560382 1102 24-25 0.23892904521331815 1102 26-27 0.1616067532721459 1102 28-29 0.19072750065769384 1102 30-31 0.1646229482597832 1102 32-33 0.11694997060400425 1102 34-35 0.2627142373114779 1102 36-37 0.23610528717973978 1102 38-39 0.12267025846362145 1102 40-41 0.24347570215103786 1102 42-43 0.34637364623921485 1102 44-45 0.1150804931333056 1102 46-47 0.3554736315596969 1102 48-49 0.2986149758586052 1102 50-51 0.2631975166451923 1102 52-53 0.2506876578977 1102 54-55 0.3229810946871723 1102 56-57 0.32083375271328407 1102 58-59 0.3705489947298233 1102 60-61 0.17599846094984173 1102 62-63 0.33078930946025764 1102 64-65 0.3890195272314969 1102 66-67 0.11281764093727986 1102 68-69 0.04696082177568783 1102 70-71 0.16291432376237225 1102 72-73 0.11859393921406536 1102 74-75 0.16813863300635035 1102 76-77 0.14964267962311695 1102 78-79 -0.021663842422686486 1102 80-81 -0.08652667465858244 1102 82-83 -0.03499600175170414 1102 84-85 -0.34626784766688345 1102 86-87 -0.2086286717332051 1102 88-89 -0.24908298809002005 1102 90-91 -0.5704526620002319 1102 92-93 -0.5339097751207049 1102 94-95 -0.6889108236870953 1102 96-97 -0.6323917659026641 1102 98-99 -0.7023512903616229 1102 100-101 -0.8295700794074143 1103 1 0.13079513220193917 1103 2 0.0655529157399144 1103 3 0.03100525102821905 1103 4 0.05538252516728903 1103 5 0.057687258222102855 1103 6 0.05529088049832609 1103 7 0.05379793648554454 1103 8 0.034546356933404354 1103 9 0.12875591995704383 1103 10-11 0.06317267038921415 1103 12-13 0.08408812295225232 1103 14-15 0.10520502778773988 1103 16-17 0.17000518116373797 1103 18-19 0.12149507348704702 1103 20-21 0.20025189052739023 1103 22-23 0.25262897209346136 1103 24-25 0.17085890478279708 1103 26-27 0.10283525010850525 1103 28-29 0.21589004753022323 1103 30-31 0.2090396086906381 1103 32-33 0.13100722645804552 1103 34-35 0.0491985965054198 1103 36-37 0.12016973500538342 1103 38-39 0.2629670554726502 1103 40-41 0.19632810183065175 1103 42-43 -0.06781220594705673 1103 44-45 0.10252052539325263 1103 46-47 0.16990656053517483 1103 48-49 0.07650673045024803 1103 50-51 0.04531507420174563 1103 52-53 -0.043730388269494824 1103 54-55 -0.028699734204877814 1103 56-57 0.028383105712990186 1103 58-59 0.23684414548408483 1103 60-61 0.14423980910501655 1103 62-63 0.11835142509163887 1103 64-65 -0.11723880450823287 1103 66-67 -0.08859948232951353 1103 68-69 -0.11839597761680665 1103 70-71 -0.1154589742069696 1103 72-73 -0.2711402488414407 1103 74-75 -0.4067801628441998 1103 76-77 -0.31089743882962395 1103 78-79 -0.47598131184641446 1103 80-81 -0.3669380443480925 1103 82-83 -0.17589005112271394 1103 84-85 -0.3254509699525734 1103 86-87 -0.6161470535257401 1103 88-89 -0.8834838234118862 1103 90-91 -0.9554289243367506 1103 92-93 -0.7264512571380024 1103 94-95 -0.8918508711901367 1103 96-97 -0.957523618715296 1103 98-99 -0.7876153008488735 1103 100-101 -0.8037531185489222 1104 1 0.09039749600795233 1104 2 0.06887784868300173 1104 3 0.06557511648905745 1104 4 0.08372786095693385 1104 5 0.030017808261582957 1104 6 -0.01185242856426072 1104 7 -0.03226951120475263 1104 8 -0.0061084747530770755 1104 9 0.006538216932106877 1104 10-11 0.006952526997743291 1104 12-13 0.044039063078201934 1104 14-15 0.21029663457503744 1104 16-17 0.08267435199736894 1104 18-19 0.10409782541236012 1104 20-21 0.0774307417474418 1104 22-23 0.11674767545601128 1104 24-25 0.15271299851094255 1104 26-27 0.05826931900765686 1104 28-29 0.006396676129291734 1104 30-31 -0.058660691437232515 1104 32-33 -0.031750999145600645 1104 34-35 0.014433009926897 1104 36-37 -0.038578699890265966 1104 38-39 0.10035636836857265 1104 40-41 0.08238750800813222 1104 42-43 0.13677384887345312 1104 44-45 0.1301936305868736 1104 46-47 0.24450122841404465 1104 48-49 0.18013425023502805 1104 50-51 0.3631758058332082 1104 52-53 0.40407454456726555 1104 54-55 0.41628933997110096 1104 56-57 0.3505835959129797 1104 58-59 0.2791886917433857 1104 60-61 0.2589277319490293 1104 62-63 0.2467202208318966 1104 64-65 0.06744018627526316 1104 66-67 0.0018090531049779202 1104 68-69 -0.11201659897984939 1104 70-71 -0.34219374783505785 1104 72-73 -0.24206486331403454 1104 74-75 -0.22684134480037343 1104 76-77 -0.19296599505242895 1104 78-79 -0.3129143851929861 1104 80-81 -0.303423717163529 1104 82-83 -0.40660670276970023 1104 84-85 -0.4422356674188954 1104 86-87 -0.5915772412319278 1104 88-89 -0.6316033721301615 1104 90-91 -0.694011305315712 1104 92-93 -0.6722016166800202 1104 94-95 -0.6292060907300794 1104 96-97 -0.9708512631767512 1104 98-99 -1.1037178653604158 1104 100-101 -1.1946434137056734 1105 1 0.1550233463480879 1105 2 0.12408265078221348 1105 3 0.17295686517515207 1105 4 0.059400738803724096 1105 5 0.06271182232205774 1105 6 0.11300442610626504 1105 7 -0.035103978324933394 1105 8 0.05222087705453049 1105 9 0.09398389754549186 1105 10-11 0.08871600161835858 1105 12-13 0.0692696123770773 1105 14-15 0.12578685518948163 1105 16-17 0.12140890936547066 1105 18-19 0.13927839708360779 1105 20-21 0.05930531989530863 1105 22-23 0.04686526318229056 1105 24-25 0.04283805633786386 1105 26-27 0.17538495009844013 1105 28-29 0.06218733129883702 1105 30-31 0.11330235242022724 1105 32-33 0.22170913630210975 1105 34-35 0.15894438192274407 1105 36-37 0.32349271730537765 1105 38-39 0.16906802248396247 1105 40-41 0.2337110809155547 1105 42-43 0.06200079583953766 1105 44-45 -0.023659159135483776 1105 46-47 0.04901969131760353 1105 48-49 0.025342958021376205 1105 50-51 -0.03677698464753831 1105 52-53 -0.2810957888949943 1105 54-55 -0.004447014771464808 1105 56-57 0.05562743061519626 1105 58-59 0.07956921184440091 1105 60-61 -0.036033173260321405 1105 62-63 -0.026144164610123255 1105 64-65 0.009698583502597558 1105 66-67 -0.1373414789026981 1105 68-69 -0.03084087339418673 1105 70-71 -0.3121730439013142 1105 72-73 -0.16052795646657358 1105 74-75 -0.34359769059398815 1105 76-77 -0.36522860132124535 1105 78-79 -0.28996657730729325 1105 80-81 -0.2097526366912028 1105 82-83 -0.11802097862496908 1105 84-85 -0.3480043293987407 1105 86-87 -0.36072648398365814 1105 88-89 -0.4144500113432592 1105 90-91 -0.2536223292124511 1105 92-93 -0.1487181987866677 1105 94-95 -0.17286541622170404 1105 96-97 -0.5393372975012944 1105 98-99 -0.7930090779435552 1105 100-101 -1.6286837522491169 1106 1 0.09343604476078582 1106 2 0.1056805697714367 1106 3 0.1397021006160415 1106 4 0.10198182921090648 1106 5 0.13686588082394024 1106 6 0.0980795442248592 1106 7 0.12584840262744734 1106 8 0.08325660461200357 1106 9 0.0494407112631734 1106 10-11 0.11339243629479512 1106 12-13 0.08107987940474004 1106 14-15 0.14073881144578593 1106 16-17 0.2028104064191396 1106 18-19 0.18757855103594068 1106 20-21 0.2662289276884806 1106 22-23 0.2688905325988671 1106 24-25 0.31362002798940125 1106 26-27 0.14606977252239517 1106 28-29 0.10505880764856812 1106 30-31 0.044570601079776395 1106 32-33 0.10960954507208243 1106 34-35 0.0012924203577426852 1106 36-37 0.04043490555191198 1106 38-39 0.13820217335723584 1106 40-41 0.04058478691969469 1106 42-43 0.007091309190919048 1106 44-45 0.02630474169798447 1106 46-47 -0.0013264359850424512 1106 48-49 0.03028164252527432 1106 50-51 -0.03384233475636478 1106 52-53 0.00406320669878113 1106 54-55 -0.16533197522164755 1106 56-57 -0.13287445397364195 1106 58-59 -0.035199063358660965 1106 60-61 -0.20481376464961443 1106 62-63 -0.18152240955358678 1106 64-65 -0.1950314690535322 1106 66-67 -0.0655832371444518 1106 68-69 -0.4551685264174914 1106 70-71 -0.9467969224382351 1106 72-73 -0.7902848179625614 1106 74-75 -0.6298232042108083 1106 76-77 -0.5799841356419968 1106 78-79 -0.5265084895013779 1106 80-81 -0.7915756446011706 1106 82-83 -0.8147359544703789 1106 84-85 -1.0664080030198058 1106 86-87 -1.134162275245533 1106 88-89 -1.2009117621528382 1106 90-91 -1.46224259783272 1106 92-93 -1.434083702847822 1106 94-95 -1.5859067710021932 1106 96-97 -1.6828240522923998 1106 98-99 -1.6674593619590574 1106 100-101 -2.0835889919369652 1107 1 0.057957498514589645 1107 2 0.05108714318210161 1107 3 0.06885288614348894 1107 4 0.052985062302163044 1107 5 -0.09051986433048143 1107 6 0.02633773661816008 1107 7 0.031085035663465987 1107 8 0.027193177535266955 1107 9 0.046230040437350794 1107 10-11 0.025118647079345635 1107 12-13 0.06800844270996009 1107 14-15 0.05000998494418951 1107 16-17 -0.02210113035523875 1107 18-19 0.08118157941890303 1107 20-21 0.03387510727939258 1107 22-23 0.042368299003960885 1107 24-25 0.09689633544176246 1107 26-27 0.15567692756456353 1107 28-29 0.11944346427478791 1107 30-31 0.12072046054591823 1107 32-33 0.136952886366295 1107 34-35 0.192327009560195 1107 36-37 0.1763763199887194 1107 38-39 0.19496664509612316 1107 40-41 0.10260506278848425 1107 42-43 0.15601399119446313 1107 44-45 0.2403563411845866 1107 46-47 0.3187619460071218 1107 48-49 0.1772826660667306 1107 50-51 0.16916508998084367 1107 52-53 0.021062605571259496 1107 54-55 0.021420798900635418 1107 56-57 -0.08585036013276692 1107 58-59 0.11640643452115285 1107 60-61 0.06888381946762223 1107 62-63 0.1518967063015566 1107 64-65 0.07525658475550046 1107 66-67 -0.004261406467456652 1107 68-69 -0.08865327889917296 1107 70-71 -0.2862767034328577 1107 72-73 -0.5184608021057926 1107 74-75 -0.34672479801106704 1107 76-77 -0.23829919144937506 1107 78-79 -0.25752390760994004 1107 80-81 -0.11332265114858586 1107 82-83 -0.0731483279492835 1107 84-85 -0.05841284453334694 1107 86-87 -0.24292850162690982 1107 88-89 -0.18706405862243258 1107 90-91 -0.5783342941495953 1107 92-93 -0.5645667169337045 1107 94-95 -0.6678740826624399 1107 96-97 -0.8168790635838477 1107 98-99 -0.9300024563035834 1107 100-101 -1.4960250205961252 1108 1 -0.06793810025716596 1108 2 -0.04717283634706604 1108 3 -0.04373667373368306 1108 4 0.033026003960301864 1108 5 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-0.5548599794837443 2316 48-49 -0.2984689796748228 2316 50-51 -0.11025624991592053 2316 52-53 -0.05370572201091761 2316 54-55 -0.09269108146576599 2316 56-57 0.017879376013638648 2316 58-59 0.0650896374875316 2316 60-61 0.11956873631825715 2316 62-63 0.2131283287905319 2316 64-65 0.29252452895542547 2316 66-67 0.36984732532443587 2316 68-69 0.49697903179278313 2316 70-71 0.5592082763892634 2316 72-73 0.8630109068314198 2316 74-75 0.949898050144597 2316 76-77 0.8856870108653858 2316 78-79 0.850827941005786 2316 80-81 1.0228372750382313 2316 82-83 1.0018814892550765 2316 84-85 1.2790186879818002 2316 86-87 1.3858299818198923 2316 88-89 1.5582575315809173 2316 90-91 1.6637814052644515 2316 92-93 1.7361137419837682 2316 94-95 1.940279833721 2316 96-97 2.35225033518617 2316 98-99 3.0115274197454944 2316 100-101 3.78107004240848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 16.0 5 28.0 6 58.0 7 159.0 8 399.0 9 762.0 10 1372.0 11 2343.0 12 3609.0 13 5535.0 14 7502.0 15 10427.0 16 13738.0 17 19686.0 18 19913.0 19 15592.0 20 14173.0 21 14394.0 22 15209.0 23 16649.0 24 19088.0 25 21755.0 26 24977.0 27 29380.0 28 35021.0 29 42486.0 30 51562.0 31 62524.0 32 77769.0 33 100362.0 34 136237.0 35 191212.0 36 272870.0 37 345173.0 38 203041.0 39 12061.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.993523994573856 19.02676360901313 15.050500754377405 22.92921164203561 2 15.522276296820294 20.149571979906337 40.243290919347764 24.084860803925608 3 19.271049020551516 23.954684138777026 30.35238208228218 26.421884758389275 4 12.7081740889918 17.083732959689684 37.14393151756546 33.06416143375305 5 13.262965047893932 37.68640806144569 34.48732397203473 14.563302918625642 6 31.948746989461995 36.50070283192021 17.077864375822582 14.472685802795212 7 28.233808295954088 33.33501204193638 20.293796388314398 18.137383273795134 8 26.412409461649343 34.959442400150415 19.42299427896108 19.205153859239164 9 26.14560670767192 16.825024844887327 20.34617209672943 36.68319635071132 10-11 24.964859033242906 26.572194542182594 27.068168998952487 21.394777425622017 12-13 26.15711965485548 23.940176069588475 27.59443840572107 22.30826586983497 14-15 22.542594432954616 25.50280120508895 26.31977272523793 25.634831636718502 16-17 21.620250642891097 28.583227781960662 27.414398466294486 22.38212310885375 18-19 21.641995245897235 26.825679541242515 30.057557322303097 21.474767890557153 20-21 22.35004655618526 25.917722014808447 30.278839094661258 21.453392334345033 22-23 21.872677786432455 26.106017163116757 30.21457253364132 21.80673251680947 24-25 21.864795408029792 26.160576262602948 30.32642103599123 21.64820729337603 26-27 21.411609247638726 26.506228074692125 30.199642031220968 21.882520646448185 28-29 21.403413148051563 27.04340079195421 29.936199336083607 21.616986723910614 30-31 21.67648090769652 26.496911913572752 29.826376628471053 22.000230550259676 32-33 21.291536846534697 26.566822674652848 29.85088031479144 22.290760164021023 34-35 22.11331051755771 26.773643945746915 29.574186666845748 21.538858869849626 36-37 22.174844977084053 26.276576606067625 29.681219221694448 21.867359195153867 38-39 21.67290741780633 26.65496018705746 29.589889899216278 22.08224249591994 40-41 22.212522837853342 26.39918749003248 29.609553147496857 21.77873652461732 42-43 21.82013273345868 26.562919688423314 29.321028068761752 22.295919509356253 44-45 21.850049312079012 26.69194989053418 29.550595591989758 21.907405205397048 46-47 22.347202287524095 26.981369101078013 28.79367797278809 21.877750638609804 48-49 22.068634983271565 27.065761576026105 28.654920856795762 22.210682583906568 50-51 22.133047865961654 27.343172481870564 28.620249119899093 21.90353053226869 52-53 22.527828283082656 27.068688893477738 28.42600846922198 21.977474354217627 54-55 21.870289656978603 27.19878497458111 28.684074084540857 22.24685128389943 56-57 22.203131388064392 27.28028276572459 28.272095129813163 22.244490716397856 58-59 22.1878004038267 27.08440201885364 28.27357789615525 22.454219681164407 60-61 21.81835020405468 27.276707996156734 28.684413893289445 22.22052790649914 62-63 21.880322267401347 27.25381496209524 28.743420187327022 22.122442583176387 64-65 21.527908761250508 27.412650270303246 28.545270966481727 22.51417000196452 66-67 21.657540253177878 27.361107963107074 28.306936270127288 22.674415513587764 68-69 22.15426880669548 26.977254077484808 28.50598202760901 22.3624950882107 70-71 21.97304435279639 27.116354663426584 28.072904045082037 22.837696938694986 72-73 21.908977285032094 27.234038624091244 28.254719449175386 22.602264641701268 74-75 21.86509483355193 27.77262110204985 27.902071230030863 22.460212834367358 76-77 21.868361615596843 27.172520827963858 28.307449318458033 22.651668237981262 78-79 21.927596000031343 27.431974673880312 28.144131860603355 22.49629746548499 80-81 22.273789693327135 27.446937286760686 27.75020475730907 22.529068262603104 82-83 22.111198186347224 27.547930252735874 27.93022474740407 22.410646813512834 84-85 22.025744798624945 27.664436659724593 27.870851758921937 22.43896678272852 86-87 22.137668859114537 27.687759076837736 27.531952635065604 22.642619428982126 88-89 21.99630153876808 27.786481027196114 27.321822656321277 22.895394777714532 90-91 21.978744393991338 27.676534174788532 27.52815073429093 22.8165706969292 92-93 22.23357808450422 27.38826890296307 27.741165072290542 22.63698794024217 94-95 21.848224049679374 27.225072727048865 27.856474470259425 23.070228753012337 96-97 22.034815097959438 26.830313894418605 28.038471892102745 23.096399115519212 98-99 21.80009899940091 27.164814415297727 27.526814203196132 23.50827238210523 100-101 22.207497711109568 26.603090729602012 27.639079474305106 23.55033208498331 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 725.0 1 489.0 2 341.0 3 439.5 4 378.0 5 376.5 6 715.0 7 1559.0 8 2534.5 9 2924.0 10 2406.0 11 1763.0 12 1673.5 13 1748.5 14 1879.0 15 2045.0 16 2192.5 17 2409.0 18 2607.0 19 2889.5 20 3332.0 21 3989.0 22 4891.5 23 6034.5 24 7438.5 25 9231.5 26 11819.0 27 14867.5 28 17319.0 29 19738.5 30 22612.0 31 26705.5 32 32891.0 33 35906.5 34 36212.0 35 38771.0 36 42551.0 37 45574.5 38 47632.5 39 49271.0 40 51154.0 41 53947.5 42 55863.5 43 57313.0 44 59390.5 45 62873.5 46 64806.0 47 66693.5 48 70166.0 49 69007.0 50 64901.0 51 62127.5 52 60094.5 53 59582.0 54 60199.0 55 56946.0 56 51424.0 57 46258.0 58 42336.5 59 36951.0 60 29271.5 61 24172.0 62 20416.0 63 15082.5 64 10155.5 65 7370.5 66 5370.5 67 4114.5 68 3109.5 69 2254.5 70 1641.0 71 1098.5 72 750.0 73 559.0 74 411.5 75 277.5 76 192.5 77 119.5 78 64.5 79 40.5 80 26.5 81 16.0 82 7.0 83 4.5 84 3.5 85 1.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01029607943201454 2 0.01432498007932458 3 0.009176940363317308 4 1.1191390686972328E-4 5 7.833973480880627E-4 6 0.0017906225099155724 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.875480110660472E-4 14-15 0.0 16-17 0.002434127474416481 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00310561091563482 26-27 0.019025364167852953 28-29 0.006491006598443949 30-31 0.003301460252656836 32-33 0.0 34-35 0.00931683274690446 36-37 7.55418871370632E-4 38-39 0.002434127474416481 40-41 0.0018465794633504339 42-43 0.0026859337648733584 44-45 2.797847671743082E-5 46-47 1.678708603045849E-4 48-49 6.155264877834779E-4 50-51 0.004308685414484346 52-53 0.00822567215492466 54-55 0.007246425469814581 56-57 0.01726272013465481 58-59 0.02408946845370793 60-61 0.0037491158801357294 62-63 0.02087194363120339 64-65 0.021879168793030895 66-67 0.013513604254519085 68-69 0.03189546345787113 70-71 0.01684304298389335 72-73 0.005287932099594424 74-75 0.01675910755374106 76-77 0.02056418038731165 78-79 0.02652359592812441 80-81 0.012114680418647543 82-83 0.035700536291441716 84-85 0.022214910513640065 86-87 0.032259183655197726 88-89 0.02232682442050979 90-91 0.028006455194148242 92-93 0.024816908848361133 94-95 0.015108377427412641 96-97 0.015500076101456671 98-99 0.01166702479116865 100-101 0.01096756287323288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787088.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.62141576544118 #Duplication Level Percentage of deduplicated Percentage of total 1 77.919802023966 46.45688912831742 2 13.163772813784364 15.696855439448981 3 4.1603602969836775 7.441397130014946 4 1.7341730628472436 4.135754127569763 5 0.9040758645883394 2.6951141503061042 6 0.519594494624205 1.8587375636054413 7 0.33077137606680296 1.3804740415050172 8 0.2270947789465811 1.0831769786988055 9 0.15992829325179314 0.8581636138160302 >10 0.8014097406567101 8.480358400520036 >50 0.04579986531220344 1.8806805172370866 >100 0.02655599683737795 3.0854431463053436 >500 0.003940541826235692 1.6010648134098353 >1k 0.0025332054597229457 2.451438207772784 >5k 1.8764484886836633E-4 0.8944527414724082 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8625 0.4826287233756815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7365 0.4121229620477559 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4600 0.2574019858003635 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2419 0.1353598703589303 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2296 0.1284771650864423 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2223 0.1243923074856974 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2188 0.12243381411547725 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2118 0.11851682737503692 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2112 0.11818108565442777 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1994 0.11157816514911409 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.1019535691583179 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.595695343486163E-5 10-11 0.0 0.0 0.0 5.595695343486163E-5 5.595695343486163E-5 12-13 0.0 0.0 0.0 5.595695343486163E-5 2.2382781373944652E-4 14-15 0.0 0.0 0.0 5.595695343486163E-5 2.2382781373944652E-4 16-17 0.0 0.0 0.0 5.595695343486163E-5 3.357417206091698E-4 18-19 2.7978476717430815E-5 0.0 0.0 5.595695343486163E-5 3.357417206091698E-4 20-21 5.595695343486163E-5 0.0 0.0 5.595695343486163E-5 3.357417206091698E-4 22-23 5.595695343486163E-5 0.0 0.0 5.595695343486163E-5 4.4765562747889305E-4 24-25 1.1191390686972326E-4 0.0 0.0 1.678708603045849E-4 5.036125809137547E-4 26-27 1.1191390686972326E-4 0.0 0.0 0.0010072251618275093 5.036125809137547E-4 28-29 1.678708603045849E-4 0.0 0.0 0.003161567869069682 5.036125809137547E-4 30-31 1.678708603045849E-4 0.0 0.0 0.012814142336583314 5.036125809137547E-4 32-33 1.678708603045849E-4 0.0 0.0 0.036511912116247214 5.315910576311855E-4 34-35 1.678708603045849E-4 0.0 0.0 0.07179277125692747 5.595695343486163E-4 36-37 1.678708603045849E-4 0.0 0.0 0.1255674035078295 5.595695343486163E-4 38-39 2.2382781373944652E-4 0.0 0.0 0.20824380220783756 5.875480110660472E-4 40-41 2.2382781373944652E-4 0.0 0.0 0.2919554045463906 6.155264877834779E-4 42-43 2.2382781373944652E-4 0.0 0.0 0.3748836095368555 6.155264877834779E-4 44-45 2.2382781373944652E-4 0.0 0.0 0.4774806836596742 6.435049645009088E-4 46-47 2.2382781373944652E-4 0.0 0.0 0.5972005855335607 6.714834412183396E-4 48-49 2.2382781373944652E-4 0.0 0.0 0.7169204874074473 6.714834412183396E-4 50-51 2.2382781373944652E-4 0.0 0.0 0.8468245548064784 7.274403946532012E-4 52-53 2.2382781373944652E-4 0.0 0.0 0.9718603672566768 7.55418871370632E-4 54-55 2.797847671743082E-4 0.0 0.0 1.1054296151056915 7.833973480880628E-4 56-57 3.357417206091698E-4 0.0 0.0 1.2603744191668234 7.833973480880628E-4 58-59 3.357417206091698E-4 0.0 0.0 1.417641436795502 7.833973480880628E-4 60-61 3.357417206091698E-4 0.0 0.0 1.592283088465705 7.833973480880628E-4 62-63 3.357417206091698E-4 0.0 0.0 1.769582695424064 8.953112549577861E-4 64-65 3.357417206091698E-4 0.0 0.0 1.9425736169679388 8.953112549577861E-4 66-67 3.357417206091698E-4 0.0 0.0 2.1432072735086356 8.953112549577861E-4 68-69 3.357417206091698E-4 2.7978476717430815E-5 0.0 2.357438469734003 8.953112549577861E-4 70-71 3.357417206091698E-4 5.595695343486163E-5 0.0 2.58605060299213 8.953112549577861E-4 72-73 3.357417206091698E-4 5.595695343486163E-5 0.0 2.817656433259023 8.953112549577861E-4 74-75 3.357417206091698E-4 5.595695343486163E-5 0.0 3.0441701807633423 9.232897316752168E-4 76-77 3.357417206091698E-4 5.595695343486163E-5 0.0 3.2826587163027225 9.512682083926477E-4 78-79 3.357417206091698E-4 5.595695343486163E-5 0.0 3.5578270348186547 9.512682083926477E-4 80-81 3.357417206091698E-4 5.595695343486163E-5 0.0 3.8432354758131666 0.0010352036385449402 82-83 3.357417206091698E-4 5.595695343486163E-5 0.0 4.1314697429561384 0.0011750960221320942 84-85 3.357417206091698E-4 5.595695343486163E-5 0.0 4.429160735229603 0.0012310529755669558 86-87 3.357417206091698E-4 5.595695343486163E-5 0.0 4.733062949334336 0.0012590314522843867 88-89 3.357417206091698E-4 5.595695343486163E-5 0.0 5.050478767693589 0.0012870099290018175 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6970 0.0 33.062164 1 GTATCAA 10075 0.0 29.758202 1 TATCAAC 12050 0.0 24.75906 2 ATCAACG 12085 0.0 24.643904 3 TCAACGC 12140 0.0 24.528137 4 CAACGCA 12190 0.0 24.466488 5 AACGCAG 12590 0.0 23.763935 6 GAGTACT 7890 0.0 23.086212 12-13 GTACATG 6855 0.0 21.972229 1 GTACTTT 8240 0.0 21.90325 14-15 TACATGG 6725 0.0 21.75805 2 TAGCCCT 1460 0.0 21.468752 4 CGCAGAG 13985 0.0 21.291616 8 ACGCAGA 14035 0.0 21.283438 7 ACATGGG 6640 0.0 21.031385 3 ACCGCTA 160 4.9992537E-5 20.77694 8 AGAGTAC 11340 0.0 20.564615 10-11 GTGTAGC 1250 0.0 20.526083 1 TAAGGTG 1065 0.0 20.066822 5 AGTACTT 8255 0.0 20.022877 12-13 >>END_MODULE