FastQCFastQC Report
Thu 26 May 2016
SRR938071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819306
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106930.5877515931899306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87890.4830963015567475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56370.3098434238110576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.11779216910184434No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18210.1000931124285854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.046.2134171
GTATCAA92150.036.498461
TATCAAC112900.029.3809992
ATCAACG112900.029.2126263
TCAACGC114150.028.9343664
CAACGCA115300.028.6457735
AACGCAG118050.027.972316
GTACATG77700.025.7874891
TACATGG76400.025.627662
ACGCAGA131150.025.028557
CGCAGAG131700.024.7797498
GAGTACT81750.024.58048612-13
ACATGGG77350.024.329883
GTACTTT85800.023.31044414-15
GCAGAGT143350.022.1350659
GTATAGG5950.021.6484891
AGTACTT84750.021.3561612-13
CAGAGTA135400.020.83952110-11
AGAGTAC122950.020.3418210-11
ACTTTTT101950.019.8954916-17
Produced by FastQC (version 0.11.5)