FastQCFastQC Report
Thu 26 May 2016
SRR938071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819306
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103910.5711518568069363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.4983768535914244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58260.3202320005540574No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.11999080968237338No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18300.10058780655920443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56950.045.842291
GTATCAA93100.037.5426861
ATCAACG116500.029.5496643
TCAACGC117050.029.4108124
TATCAAC118000.029.1772422
CAACGCA118850.028.9653845
AACGCAG123000.027.956566
ACGCAGA137200.024.8553927
CGCAGAG136650.024.851168
GAGTACT83500.023.7476312-13
GTAAGGT11600.023.3645154
GTATAGG4950.023.0565191
GCAGAGT147500.022.7977249
TAGCCCG1050.002183629722.6423034
TAAGGTG12900.022.4843355
GTACTTT87900.022.39679714-15
GTACATG79850.021.8564111
TACATGG76850.021.8433152
GGTAAGG11800.021.7596383
CTACTCG1100.002860893321.6131084
Produced by FastQC (version 0.11.5)