##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938071_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1819306 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46756290585531 33.0 31.0 34.0 31.0 34.0 2 32.695892279803395 34.0 31.0 34.0 31.0 34.0 3 32.75244901077664 34.0 31.0 34.0 31.0 34.0 4 36.22403377991388 37.0 35.0 37.0 35.0 37.0 5 36.15260544405394 37.0 35.0 37.0 35.0 37.0 6 36.207061373952484 37.0 37.0 37.0 35.0 37.0 7 36.1748837193963 37.0 37.0 37.0 35.0 37.0 8 36.17015554282787 37.0 37.0 37.0 35.0 37.0 9 37.85562516695927 39.0 38.0 39.0 35.0 39.0 10-11 37.82907878058996 39.0 38.0 39.0 35.0 39.0 12-13 37.750534269661074 39.0 38.0 39.0 35.0 39.0 14-15 38.983119936942984 40.0 38.0 41.0 35.5 41.0 16-17 38.896913438421024 40.0 38.0 41.0 35.5 41.0 18-19 39.157270959365825 40.0 38.5 41.0 36.0 41.0 20-21 39.13942266996316 40.0 39.0 41.0 36.0 41.0 22-23 39.033261859192464 40.0 39.0 41.0 35.0 41.0 24-25 38.92608610096377 40.0 38.0 41.0 35.0 41.0 26-27 38.76879535383273 40.0 38.0 41.0 35.0 41.0 28-29 38.64664685325064 40.0 38.0 41.0 34.5 41.0 30-31 38.39078197950208 40.0 38.0 41.0 34.0 41.0 32-33 38.150159731238176 40.0 38.0 41.0 34.0 41.0 34-35 38.04670214906123 40.0 37.5 41.0 33.5 41.0 36-37 37.98739574321197 40.0 37.5 41.0 33.0 41.0 38-39 37.80259945275836 40.0 37.0 41.0 33.0 41.0 40-41 37.58093910535116 40.0 37.0 41.0 33.0 41.0 42-43 37.31664766674765 40.0 37.0 41.0 32.5 41.0 44-45 37.026457066595725 40.0 36.0 41.0 31.0 41.0 46-47 36.62870044951207 39.5 36.0 41.0 30.5 41.0 48-49 36.19273832989063 39.0 35.0 41.0 30.0 41.0 50-51 35.80710501696801 39.0 35.0 40.0 28.5 41.0 52-53 35.28595244560289 38.0 35.0 40.0 27.0 41.0 54-55 34.92330317164897 38.0 34.0 40.0 26.0 41.0 56-57 34.55790862010019 38.0 34.0 40.0 25.5 41.0 58-59 34.071821342863714 37.5 33.5 40.0 23.5 41.0 60-61 33.60325640656382 37.0 33.0 40.0 22.5 41.0 62-63 33.05416213655097 36.0 32.0 39.0 21.0 40.5 64-65 32.498148744631195 36.0 31.5 39.0 18.5 40.0 66-67 31.940719977837702 35.0 31.0 38.5 15.5 40.0 68-69 31.33319903303787 35.0 30.5 38.0 9.5 40.0 70-71 30.722896807903673 35.0 29.5 37.5 7.5 39.5 72-73 30.05653529422758 34.0 29.0 37.0 4.0 39.0 74-75 29.41588578282048 34.0 28.0 36.0 2.0 39.0 76-77 28.12301586429111 32.5 26.5 35.0 2.0 37.0 78-79 28.77519642105286 34.0 28.0 35.0 2.0 37.0 80-81 28.50293765864566 34.0 28.0 35.0 2.0 37.0 82-83 27.952008348238287 33.5 26.5 35.0 2.0 36.5 84-85 27.293364887490064 33.0 26.0 35.0 2.0 36.0 86-87 26.593065982303145 32.5 25.0 35.0 2.0 35.5 88-89 25.80072511166346 32.0 22.0 34.5 2.0 35.0 90-91 25.157254469561472 32.0 19.5 34.0 2.0 35.0 92-93 24.2384513655207 31.0 12.0 34.0 2.0 35.0 94-95 23.349505250903366 31.0 2.0 34.0 2.0 35.0 96-97 22.322479835717576 30.0 2.0 34.0 2.0 35.0 98-99 20.930183817345736 29.5 2.0 34.0 2.0 35.0 100-101 17.06851211395994 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2659270350386578 1101 2 0.17536956975382623 1101 3 0.19665807446278905 1101 4 0.09273172367194604 1101 5 0.04242229498827044 1101 6 0.11703984285174585 1101 7 0.14896595562633053 1101 8 0.15180843034760017 1101 9 0.10761702827127806 1101 10-11 0.21973936586586973 1101 12-13 0.2982162899065415 1101 14-15 0.39971603930349886 1101 16-17 0.36081200212739617 1101 18-19 0.33242279792955287 1101 20-21 0.22392765776206147 1101 22-23 0.27297841956046653 1101 24-25 0.28463743469949776 1101 26-27 0.40682976844666996 1101 28-29 0.427724365288519 1101 30-31 0.5458430868250019 1101 32-33 0.4690532583495255 1101 34-35 0.46345412215298865 1101 36-37 0.6085286078016807 1101 38-39 0.6600150363483195 1101 40-41 0.7671926028416394 1101 42-43 0.821164787740642 1101 44-45 0.8301001322485959 1101 46-47 0.7074046437738488 1101 48-49 0.7478378940790122 1101 50-51 0.8202727567830266 1101 52-53 0.8621045934163263 1101 54-55 1.0745219171700597 1101 56-57 1.0028302167254992 1101 58-59 1.2357323640580873 1101 60-61 1.1803612861222348 1101 62-63 1.1680186847688816 1101 64-65 1.292704311581467 1101 66-67 1.4740293247918679 1101 68-69 1.6200911468334915 1101 70-71 1.7423756714967986 1101 72-73 1.739811845727072 1101 74-75 1.9501719181605353 1101 76-77 2.1334868271982117 1101 78-79 2.1715902618620575 1101 80-81 2.3137349560420724 1101 82-83 2.3417724043887205 1101 84-85 2.461434242111711 1101 86-87 2.6438579455291347 1101 88-89 3.1875387624404112 1101 90-91 3.519121263295535 1101 92-93 3.5509905527865833 1101 94-95 3.7335041836756027 1101 96-97 3.7904975843360056 1101 98-99 4.034881678860206 1101 100-101 3.8782598263840917 1102 1 0.17634521854220253 1102 2 0.15277657804040246 1102 3 0.15530360566229717 1102 4 0.06203754697799013 1102 5 -0.04848828081696155 1102 6 0.054568963057093356 1102 7 0.036516330263772545 1102 8 0.0994743425261646 1102 9 0.1430668075804462 1102 10-11 0.2022856182460231 1102 12-13 0.30149878274720976 1102 14-15 0.35839913837528314 1102 16-17 0.31393648531393836 1102 18-19 0.300352289566824 1102 20-21 0.23806951159772183 1102 22-23 0.39926465979639403 1102 24-25 0.3663673617157386 1102 26-27 0.33899872913556806 1102 28-29 0.35106548851073427 1102 30-31 0.41658530956082984 1102 32-33 0.3409533474098865 1102 34-35 0.35637874425898275 1102 36-37 0.4295103461406313 1102 38-39 0.4466805650895722 1102 40-41 0.41854110570067604 1102 42-43 0.5423278727326419 1102 44-45 0.5398891355606921 1102 46-47 0.44145595903731305 1102 48-49 0.4730142078622137 1102 50-51 0.4448131118239189 1102 52-53 0.42646305461993705 1102 54-55 0.6232586770472466 1102 56-57 0.536985129480243 1102 58-59 0.6311054314054374 1102 60-61 0.6698230896006123 1102 62-63 0.8709631892629375 1102 64-65 0.8200245258326788 1102 66-67 1.0594651999048317 1102 68-69 1.2868483983516263 1102 70-71 1.427474415790666 1102 72-73 1.569935235770199 1102 74-75 1.4851966088278985 1102 76-77 1.4328848020448888 1102 78-79 1.663282861930412 1102 80-81 1.8481434519347175 1102 82-83 2.062154322645114 1102 84-85 2.112849917959771 1102 86-87 2.1786276238460616 1102 88-89 2.378947275891953 1102 90-91 2.29733585833225 1102 92-93 2.779996135290734 1102 94-95 3.025926172151248 1102 96-97 3.2281245035132216 1102 98-99 3.646914790856158 1102 100-101 3.562940015579162 1103 1 0.25311725600356993 1103 2 0.2487361857729269 1103 3 0.2356312566962302 1103 4 0.15150714982310376 1103 5 0.006034364851693397 1103 6 0.11575139105320176 1103 7 0.12169021421991744 1103 8 0.16349792022245424 1103 9 0.2917813553702686 1103 10-11 0.2894364186864351 1103 12-13 0.30617718076302225 1103 14-15 0.47498651081880894 1103 16-17 0.3311104252150585 1103 18-19 0.2853211585227271 1103 20-21 0.18587479824186914 1103 22-23 0.2213602417755851 1103 24-25 0.3081169939941262 1103 26-27 0.38647910760328585 1103 28-29 0.5120702523802336 1103 30-31 0.39279043800503644 1103 32-33 0.5096472421820621 1103 34-35 0.5596423561749546 1103 36-37 0.46773513457929283 1103 38-39 0.4754112203683363 1103 40-41 0.5366624969588045 1103 42-43 0.6078514749048622 1103 44-45 0.5540150459290842 1103 46-47 0.6861742100732613 1103 48-49 0.7777233367601966 1103 50-51 0.9255352137385557 1103 52-53 0.8962199426499708 1103 54-55 1.1766363933891597 1103 56-57 1.2472150555318748 1103 58-59 1.4911421919120258 1103 60-61 1.4013727108454006 1103 62-63 1.3086267020319866 1103 64-65 1.1903755572014845 1103 66-67 1.3102729053918658 1103 68-69 1.5696292362673923 1103 70-71 1.6697604204574112 1103 72-73 1.9149987527351193 1103 74-75 2.0018680152766457 1103 76-77 2.0777914011424663 1103 78-79 1.9599444624693518 1103 80-81 2.34171813341748 1103 82-83 2.512108634253927 1103 84-85 2.6978287715385783 1103 86-87 2.850648495981506 1103 88-89 3.1475031342237543 1103 90-91 3.3394486278490767 1103 92-93 3.5124288323277497 1103 94-95 3.76635433764579 1103 96-97 4.113876524330966 1103 98-99 4.423301196539544 1103 100-101 4.075780162931995 1104 1 0.2064119946754701 1104 2 0.2286159130138472 1104 3 0.27509946890965864 1104 4 0.1822673590352153 1104 5 0.0490066818736139 1104 6 0.11409340488478392 1104 7 0.2024177477922109 1104 8 0.20262947432869538 1104 9 0.1893305723651224 1104 10-11 0.2288693148042995 1104 12-13 0.2619495470734492 1104 14-15 0.4849773352351079 1104 16-17 0.52119243336967 1104 18-19 0.38312859008541267 1104 20-21 0.3401115194999562 1104 22-23 0.3929255039398356 1104 24-25 0.4436548878346045 1104 26-27 0.4975680315515518 1104 28-29 0.4658276209020329 1104 30-31 0.4601583657093613 1104 32-33 0.4888690558670987 1104 34-35 0.6239748599651094 1104 36-37 0.7658172835582064 1104 38-39 0.6592182954694934 1104 40-41 0.7349095644026491 1104 42-43 0.7651148185579757 1104 44-45 0.8862102328183425 1104 46-47 0.9018394838995292 1104 48-49 0.936535197687391 1104 50-51 1.2763643168417573 1104 52-53 1.2315693739618112 1104 54-55 1.30685706947785 1104 56-57 1.218869763134407 1104 58-59 1.3677608462917732 1104 60-61 1.3671920112566553 1104 62-63 1.5742989070545832 1104 64-65 1.6267867817788542 1104 66-67 1.7972230029787077 1104 68-69 2.0982759806954796 1104 70-71 2.121764636385084 1104 72-73 2.262456323911909 1104 74-75 2.169275659836096 1104 76-77 2.1139265880757314 1104 78-79 1.9915468670443772 1104 80-81 2.201181526799175 1104 82-83 2.294654451662012 1104 84-85 2.514966209460365 1104 86-87 2.6604592193123544 1104 88-89 2.7296933702584916 1104 90-91 3.1378481816120605 1104 92-93 3.5003223427476478 1104 94-95 3.788306426576341 1104 96-97 4.2257861899392495 1104 98-99 4.392751123596955 1104 100-101 3.970062916135202 1105 1 0.16158532964421823 1105 2 0.18029146878653535 1105 3 0.1184613284600502 1105 4 0.10027795943707218 1105 5 -0.008365212612993389 1105 6 -0.09648433676601798 1105 7 0.08096044027487892 1105 8 0.0923952045468397 1105 9 0.0968086822269214 1105 10-11 0.09057519852279938 1105 12-13 0.20487805620873445 1105 14-15 0.2904060479310928 1105 16-17 0.2167664520251762 1105 18-19 0.18088678907734135 1105 20-21 0.17233072613641554 1105 22-23 0.2250336617172195 1105 24-25 0.30627947997691507 1105 26-27 0.29164290793390535 1105 28-29 0.2861226747754557 1105 30-31 0.24346687329931171 1105 32-33 0.3446408419727973 1105 34-35 0.38070093863759524 1105 36-37 0.337253056952008 1105 38-39 0.4245370611328383 1105 40-41 0.42935178556000153 1105 42-43 0.40478223328857155 1105 44-45 0.5826939512538729 1105 46-47 0.6435942360457503 1105 48-49 0.8304128540679159 1105 50-51 0.8909946315517985 1105 52-53 0.8501932897628706 1105 54-55 0.9025151803583213 1105 56-57 0.8784790236044415 1105 58-59 0.8865062748381476 1105 60-61 1.04993546898897 1105 62-63 0.9271290244753203 1105 64-65 1.2344716053670837 1105 66-67 1.1701463806518966 1105 68-69 1.476795112109766 1105 70-71 1.7043965948261324 1105 72-73 1.6395285285383991 1105 74-75 1.5371250921356179 1105 76-77 1.590436549383604 1105 78-79 1.7126160406087223 1105 80-81 1.677437340406179 1105 82-83 1.8247835143438103 1105 84-85 2.107089813596996 1105 86-87 2.344484676353769 1105 88-89 2.3404044051888135 1105 90-91 2.441162461663769 1105 92-93 2.952787887283783 1105 94-95 2.9518574884355786 1105 96-97 3.2125524922062745 1105 98-99 3.270994798402935 1105 100-101 3.4597641306043307 1106 1 0.1973963010667532 1106 2 0.11915299221372067 1106 3 0.15238947541477899 1106 4 0.12763652475322118 1106 5 -0.019448554750226776 1106 6 0.09410946440665668 1106 7 0.14666715148928944 1106 8 0.18869360399747848 1106 9 0.217622096732228 1106 10-11 0.24297899388490407 1106 12-13 0.19700555211492343 1106 14-15 0.14958675682127875 1106 16-17 0.17137166305470686 1106 18-19 0.046864055201687904 1106 20-21 0.026458215439681965 1106 22-23 0.16100822064174025 1106 24-25 -0.021642314962598164 1106 26-27 -0.09157146267514804 1106 28-29 0.11532279626930375 1106 30-31 0.34308885576194825 1106 32-33 0.28905641695562423 1106 34-35 0.2077948916775867 1106 36-37 0.3900437838021489 1106 38-39 0.18509547061992038 1106 40-41 0.30417235103462303 1106 42-43 0.29193574472483874 1106 44-45 0.28788021980809475 1106 46-47 0.2455310394370116 1106 48-49 0.2339998938439436 1106 50-51 0.2934954899322264 1106 52-53 0.22365480790771386 1106 54-55 0.20009817914338157 1106 56-57 0.282222882882877 1106 58-59 0.46118999466278154 1106 60-61 0.411544073922677 1106 62-63 0.5327223634431562 1106 64-65 0.7179931771276884 1106 66-67 0.8296351160594249 1106 68-69 0.7117742798769235 1106 70-71 0.7626078290451375 1106 72-73 1.1744736912345068 1106 74-75 1.4060682277333143 1106 76-77 1.2087821201405617 1106 78-79 1.2082524966860575 1106 80-81 1.0951873272003247 1106 82-83 1.540725289738667 1106 84-85 1.670812180349941 1106 86-87 1.7435913004095482 1106 88-89 1.7500613501468223 1106 90-91 1.9288453627255144 1106 92-93 1.8899405733542487 1106 94-95 2.3490195506615557 1106 96-97 2.696804512701757 1106 98-99 3.010532131348892 1106 100-101 3.000194509348187 1107 1 0.22892584132054594 1107 2 0.1917580473416578 1107 3 0.1409231242602118 1107 4 0.10605861753457901 1107 5 -0.020843655644760872 1107 6 0.04187184218906026 1107 7 0.035668867736433185 1107 8 0.09795613780234191 1107 9 0.1352123297257819 1107 10-11 0.23381917487097326 1107 12-13 0.18073175868866542 1107 14-15 0.3002160474391218 1107 16-17 0.2522992166224256 1107 18-19 0.1882613664145012 1107 20-21 0.21734390297868345 1107 22-23 0.24482934088305797 1107 24-25 0.3894170775899255 1107 26-27 0.36198194901613334 1107 28-29 0.38727255703485497 1107 30-31 0.41456404818965353 1107 32-33 0.38798896324228593 1107 34-35 0.36832055737344405 1107 36-37 0.4967410482082215 1107 38-39 0.40298474667567774 1107 40-41 0.4719348588357448 1107 42-43 0.4681078635099425 1107 44-45 0.5507299701721493 1107 46-47 0.5486144334457492 1107 48-49 0.7074071644909239 1107 50-51 0.7967160536934088 1107 52-53 0.9928455713776501 1107 54-55 0.9414922398881274 1107 56-57 1.024593384235068 1107 58-59 1.1044472237037652 1107 60-61 1.0669606539101864 1107 62-63 1.0814195758417355 1107 64-65 1.285034081017848 1107 66-67 1.6170219997706816 1107 68-69 1.670360066230156 1107 70-71 1.575482933060112 1107 72-73 1.6774413685800447 1107 74-75 1.6762757376147377 1107 76-77 1.6663076038102105 1107 78-79 1.7200698983919018 1107 80-81 1.9790858085816438 1107 82-83 2.0623305008890718 1107 84-85 2.241435100050378 1107 86-87 2.353059263796279 1107 88-89 2.4385150374777993 1107 90-91 2.8023839522824154 1107 92-93 2.932214860851648 1107 94-95 3.012302263372188 1107 96-97 3.1990668384766714 1107 98-99 3.315981216017086 1107 100-101 3.404950594224413 1108 1 0.15635581192196213 1108 2 0.15821128284812858 1108 3 0.10742123549084681 1108 4 0.06367133538128655 1108 5 -0.009527327662382845 1108 6 0.023213513321138635 1108 7 0.048421218891967044 1108 8 0.06857184603434519 1108 9 0.14329328420251386 1108 10-11 0.17221378074243887 1108 12-13 0.18425051408205917 1108 14-15 0.3874426545551444 1108 16-17 0.22606337242029895 1108 18-19 0.2593295549111616 1108 20-21 0.1772697150963225 1108 22-23 0.23258740953825452 1108 24-25 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50-51 -0.09105166683746546 2316 52-53 0.064264720011046 2316 54-55 0.22291288429750722 2316 56-57 0.27193422691786395 2316 58-59 0.4322619832733494 2316 60-61 0.5576325689501118 2316 62-63 0.631267219630999 2316 64-65 0.8890454354118447 2316 66-67 1.035847976621465 2316 68-69 1.3080511801781647 2316 70-71 1.3697451779082535 2316 72-73 1.4052172795232032 2316 74-75 1.7625908415244282 2316 76-77 1.7214631749006024 2316 78-79 1.7746214460526204 2316 80-81 1.7205215821419344 2316 82-83 2.0133460444945754 2316 84-85 2.2563263810267067 2316 86-87 2.2791848092611176 2316 88-89 2.7973358570176146 2316 90-91 2.9043962643885415 2316 92-93 3.007292428262847 2316 94-95 3.3548163979359344 2316 96-97 3.5695967794454546 2316 98-99 3.967531110014349 2316 100-101 4.274875213346871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 13.0 5 23.0 6 58.0 7 173.0 8 458.0 9 852.0 10 1573.0 11 2581.0 12 3907.0 13 6003.0 14 8024.0 15 11213.0 16 15042.0 17 21251.0 18 21604.0 19 17046.0 20 15774.0 21 16921.0 22 18212.0 23 20069.0 24 22800.0 25 25483.0 26 29087.0 27 33647.0 28 39945.0 29 48690.0 30 57976.0 31 69975.0 32 84525.0 33 105930.0 34 138358.0 35 191137.0 36 261162.0 37 331100.0 38 188894.0 39 9790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.838477969071725 19.462861112973847 15.241636096401596 22.457024821552835 2 15.50820953655301 20.336517608434765 40.3426966817167 23.81257617329552 3 19.110789856287326 24.590725324377285 30.303228519366442 25.995256299968943 4 12.653920951058605 17.302358408115747 37.22300824832708 32.820712392498564 5 13.164835878423405 37.83235258994947 34.53803437123554 14.464777160391579 6 31.52518765417984 36.869594086529794 17.11357749996702 14.491640759323346 7 28.055972991899107 33.56884438351767 20.346604694317502 18.028577930265715 8 26.402803711091554 35.12872786995899 19.39550476198852 19.072963656960933 9 26.305525293710897 16.77254953262398 20.405143499774088 36.51678167389104 10-11 24.932941462293865 27.0007904112887 26.77812308649562 21.288145039921815 12-13 25.883661132321883 24.48705715256257 27.487047258679958 22.142234456435585 14-15 22.65674383528664 25.717498870448402 26.280955485223483 25.344801809041467 16-17 21.472534436904635 28.971137612676923 27.25144292698505 22.304885023433393 18-19 21.457165534549986 27.039459002498756 30.100846146827415 21.402529316123843 20-21 22.15746004245575 26.16448250046996 30.457740479061794 21.220316978012495 22-23 21.910368970974048 26.186731951741958 30.293387082855354 21.609511994428633 24-25 21.659465167039794 26.387665125595216 30.488067643710732 21.464802063654258 26-27 21.285621819511075 26.777136622054503 30.27906535711488 21.658176201319538 28-29 21.362569023572725 26.971136246458816 30.221139269589614 21.44515546037885 30-31 21.441610152442745 26.61564354759452 30.159934612429133 21.7828116875336 32-33 21.22133384928099 26.59668027258746 30.154080731883475 22.027905146248074 34-35 21.899017595976932 26.779208942399823 29.928580420414608 21.393193041208637 36-37 21.9241794211494 26.335338688887727 30.00252845195529 21.73795343800758 38-39 21.507151047673283 26.75186650644743 29.860969868742625 21.880012577136657 40-41 21.83886973424396 26.595421282471886 29.988560352523645 21.577148630760504 42-43 21.662231122322353 26.510501424935224 29.754087704770022 22.0731797479724 44-45 21.614420075619428 26.82857113802648 29.750338259104137 21.806670527249956 46-47 22.127393632517016 27.114927340425414 29.024144371535083 21.73353465552249 48-49 21.801829928555176 27.16733743526378 28.941887730816035 22.08894490536501 50-51 21.951942114190796 27.372333186390858 28.80854017960695 21.8671845198114 52-53 22.263077239342916 27.228459643402488 28.704489514133414 21.80397360312119 54-55 21.85841744049654 27.371426247151387 28.80886997569403 21.961286336658045 56-57 21.95734688723081 27.5418161170931 28.546650656885642 21.954186338790453 58-59 21.973763621952546 27.12273251448629 28.59285353865705 22.31065032490411 60-61 21.718089205444276 27.46657241827378 28.801257182683948 22.014081193598 62-63 21.753075073681945 27.41916423075612 28.912425947036947 21.915334748524987 64-65 21.592134583187214 27.464950920845645 28.719742583160834 22.22317191280631 66-67 21.571793389120238 27.517693579522717 28.506725648929482 22.403787382427566 68-69 22.028459422676512 27.22075400760296 28.61926507674414 22.13152149297639 70-71 21.85492709857495 27.30656635002578 28.25558207360389 22.58292447779538 72-73 21.84852609803697 27.35928887071343 28.30797610184337 22.484208929406226 74-75 21.766376849194145 27.687151034515363 28.054708773565306 22.491763342725193 76-77 21.79262312112421 27.335066228550886 28.485999606443336 22.38631104388157 78-79 21.827554023347364 27.478445077408637 28.344736949144345 22.349263950099655 80-81 21.948252681522913 27.487135176018057 28.11391401757377 22.450698124885257 82-83 21.982507833492694 27.57774196979395 28.141071852112233 22.29867834460112 84-85 21.895695546252483 27.62085869201949 28.030756934794464 22.452688826933564 86-87 21.93672418555372 27.5842754530351 27.933044112776777 22.545956248634404 88-89 21.86039621701902 27.698089271403493 27.632817129169034 22.808697382408457 90-91 21.80358303547994 27.577802604619716 27.937342914112413 22.681271445787935 92-93 21.939602243932576 27.325254794960273 27.958765595232464 22.77637736587468 94-95 21.691244268760798 27.150855092781285 28.09376984788976 23.06413079056816 96-97 21.889601218487613 26.957829214405837 28.021807234577828 23.13076233252872 98-99 21.65424617958716 27.067216839827935 27.811649057387815 23.46688792319709 100-101 21.980799271810238 26.544627456843433 28.02008018442197 23.454493086924355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1467.0 1 922.0 2 538.0 3 697.0 4 549.0 5 537.0 6 1130.5 7 2529.5 8 4020.0 9 4501.5 10 3507.5 11 2424.5 12 2300.5 13 2375.0 14 2458.5 15 2525.5 16 2673.0 17 2841.0 18 3024.0 19 3337.0 20 3772.0 21 4391.5 22 5391.5 23 6578.5 24 7785.5 25 9584.5 26 12231.5 27 15271.5 28 17586.5 29 19866.5 30 22786.5 31 26589.5 32 32011.5 33 35418.5 34 37616.0 35 40820.0 36 45075.5 37 48825.5 38 50844.0 39 52999.5 40 54678.5 41 56563.0 42 57280.0 43 58170.5 44 60642.0 45 63156.0 46 63867.5 47 64828.0 48 66343.5 49 64658.5 50 62077.0 51 60355.5 52 59051.0 53 58315.0 54 58265.0 55 55438.5 56 50957.0 57 47221.5 58 43844.5 59 38991.0 60 31681.5 61 25779.5 62 21325.0 63 15763.0 64 10831.5 65 8001.0 66 5978.0 67 4576.5 68 3389.0 69 2508.5 70 1873.5 71 1199.0 72 765.5 73 586.0 74 429.5 75 312.5 76 232.0 77 137.5 78 74.5 79 38.0 80 20.0 81 12.5 82 7.5 83 3.0 84 2.0 85 1.0 86 0.5 87 1.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01445606181697856 3 0.00280326674017455 4 0.0015390484063703411 5 0.11801203315989725 6 1.0993202902645293E-4 7 0.0 8 1.6489804353967942E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7483007256613233E-5 18-19 0.0 20-21 0.0 22-23 1.6489804353967942E-4 24-25 4.946941306190382E-4 26-27 0.005963812574685073 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010828304859105615 36-37 6.046261596454912E-4 38-39 0.007255513915745894 40-41 0.010663406815565936 42-43 0.005524084458579261 44-45 0.0035453079361031075 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 8.244902176983971E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0993202902645293E-4 68-69 7.695242031851706E-4 70-71 0.0 72-73 1.3741503628306618E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1986405805290586E-4 82-83 0.0018963275007063134 84-85 0.004067485073978759 86-87 0.002665851703891484 88-89 0.0 90-91 0.0021161915587592194 92-93 0.0 94-95 2.7483007256613233E-5 96-97 8.244902176983971E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1819306.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.93709805571686 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26337777005062 40.59488186787681 2 14.134501053851537 15.247479386204471 3 4.6466469962595225 7.518799640026562 4 2.012916553514846 4.342835100996235 5 1.0810281034711122 2.9153759408953506 6 0.6259130880428224 2.025596136247335 7 0.42111955089105224 1.589977655671309 8 0.2799213474136156 1.2078516130669252 9 0.2148261284410159 1.042838815918779 >10 1.1777540292774118 11.762662876680388 >50 0.08979806246033399 3.3217845827223194 >100 0.04658002605567148 4.414986231360413 >500 0.003881036914139088 1.4588227747772 >1k 0.0014298557052091375 1.1632792287202678 >5k 2.042651007441625E-4 0.8204156628939218 >10k+ 1.0213255037208125E-4 0.5724124859417674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10391 0.5711518568069363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9067 0.4983768535914244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5826 0.3202320005540574 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2183 0.11999080968237338 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1830 0.10058780655920443 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.496601451322647E-5 0.0 0.0 0.0 0.0 6 5.496601451322647E-5 0.0 0.0 0.0 0.0 7 5.496601451322647E-5 0.0 0.0 0.0 0.0 8 5.496601451322647E-5 0.0 0.0 0.0 0.0 9 5.496601451322647E-5 0.0 0.0 0.0 0.0 10-11 1.3741503628306618E-4 0.0 0.0 0.0 0.0 12-13 2.198640580529059E-4 0.0 0.0 5.496601451322647E-5 0.0 14-15 2.198640580529059E-4 0.0 0.0 1.0993202902645294E-4 0.0 16-17 2.198640580529059E-4 0.0 0.0 1.0993202902645294E-4 0.0 18-19 2.198640580529059E-4 0.0 0.0 1.3741503628306618E-4 5.496601451322647E-5 20-21 2.198640580529059E-4 0.0 0.0 2.198640580529059E-4 5.496601451322647E-5 22-23 2.7483007256613235E-4 0.0 0.0 2.7483007256613235E-4 5.496601451322647E-5 24-25 3.847621015925853E-4 0.0 0.0 5.221771378756515E-4 5.496601451322647E-5 26-27 3.847621015925853E-4 0.0 0.0 0.0016214974281401808 5.496601451322647E-5 28-29 3.847621015925853E-4 0.0 0.0 0.004314832139288278 5.496601451322647E-5 30-31 3.847621015925853E-4 0.0 0.0 0.012807081381581768 5.496601451322647E-5 32-33 3.847621015925853E-4 0.0 0.0 0.03253988059183007 5.496601451322647E-5 34-35 3.847621015925853E-4 0.0 0.0 0.0616718682838401 5.496601451322647E-5 36-37 3.847621015925853E-4 0.0 0.0 0.10853039565636566 5.496601451322647E-5 38-39 4.1224510884919853E-4 0.0 0.0 0.1825146511911685 5.496601451322647E-5 40-41 4.397281161058118E-4 0.0 0.0 0.2615008140466749 5.496601451322647E-5 42-43 4.397281161058118E-4 0.0 0.0 0.3369966349805915 5.496601451322647E-5 44-45 4.397281161058118E-4 0.0 0.0 0.42870743019590984 8.244902176983971E-5 46-47 4.397281161058118E-4 0.0 0.0 0.5403434056722729 1.0993202902645294E-4 48-49 4.397281161058118E-4 0.0 0.0 0.6535459125622627 1.0993202902645294E-4 50-51 4.397281161058118E-4 0.0 0.0 0.7756254307961388 1.0993202902645294E-4 52-53 4.397281161058118E-4 0.0 0.0 0.887811066417634 1.6489804353967942E-4 54-55 4.397281161058118E-4 0.0 0.0 1.0111822859925708 1.6489804353967942E-4 56-57 4.397281161058118E-4 0.0 0.0 1.151730385102891 2.198640580529059E-4 58-59 4.397281161058118E-4 0.0 0.0 1.3017876047239991 2.7483007256613235E-4 60-61 4.397281161058118E-4 0.0 0.0 1.4644320416686363 2.7483007256613235E-4 62-63 4.397281161058118E-4 0.0 0.0 1.6200408287555805 2.7483007256613235E-4 64-65 4.397281161058118E-4 0.0 0.0 1.7838120689977388 2.7483007256613235E-4 66-67 4.397281161058118E-4 0.0 0.0 1.964787671782537 2.7483007256613235E-4 68-69 4.397281161058118E-4 0.0 0.0 2.164534168523602 2.7483007256613235E-4 70-71 4.397281161058118E-4 0.0 0.0 2.368238218309619 2.7483007256613235E-4 72-73 4.397281161058118E-4 0.0 0.0 2.579637510127488 2.7483007256613235E-4 74-75 4.946941306190382E-4 0.0 0.0 2.795544015135442 2.7483007256613235E-4 76-77 4.946941306190382E-4 0.0 0.0 3.0166997745294086 2.7483007256613235E-4 78-79 4.946941306190382E-4 0.0 0.0 3.272126843972372 2.7483007256613235E-4 80-81 4.946941306190382E-4 0.0 0.0 3.539316640521166 2.7483007256613235E-4 82-83 4.946941306190382E-4 0.0 0.0 3.816427802689597 2.7483007256613235E-4 84-85 4.946941306190382E-4 0.0 0.0 4.082545761955383 2.7483007256613235E-4 86-87 5.496601451322647E-4 0.0 0.0 4.363449579125227 3.023130798227456E-4 88-89 5.496601451322647E-4 5.496601451322647E-5 0.0 4.661337894779658 3.2979608707935884E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5695 0.0 45.84229 1 GTATCAA 9310 0.0 37.542686 1 ATCAACG 11650 0.0 29.549664 3 TCAACGC 11705 0.0 29.410812 4 TATCAAC 11800 0.0 29.177242 2 CAACGCA 11885 0.0 28.965384 5 AACGCAG 12300 0.0 27.95656 6 ACGCAGA 13720 0.0 24.855392 7 CGCAGAG 13665 0.0 24.85116 8 GAGTACT 8350 0.0 23.74763 12-13 GTAAGGT 1160 0.0 23.364515 4 GTATAGG 495 0.0 23.056519 1 GCAGAGT 14750 0.0 22.797724 9 TAGCCCG 105 0.0021836297 22.642303 4 TAAGGTG 1290 0.0 22.484335 5 GTACTTT 8790 0.0 22.396797 14-15 GTACATG 7985 0.0 21.856411 1 TACATGG 7685 0.0 21.843315 2 GGTAAGG 1180 0.0 21.759638 3 CTACTCG 110 0.0028608933 21.613108 4 >>END_MODULE