FastQCFastQC Report
Thu 26 May 2016
SRR938070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793094
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103480.5771030408891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88830.4954006873036216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.3103574045755549No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.12035063415526458No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18700.10428901106132751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.044.046031
GTATCAA95150.035.8614161
ATCAACG115950.029.349373
TATCAAC116050.029.281492
TAAGCGA650.005827666729.2484725
TCAACGC117850.028.8358674
CAACGCA120200.028.3116445
AACGCAG123350.027.547046
CGCAGAG137650.024.5375828
ACGCAGA139250.024.2897577
GTACATG81800.023.219881
GAGTACT83500.023.01156612-13
TACATGG79950.022.8268012
GCAGAGT148400.022.2159029
GTATACG1100.002800723521.6923941
GTACTTT88150.021.42136414-15
GTATAGG5350.021.4085671
ACATGGG81650.021.2467863
CAGAGTA142450.020.30723610-11
AGTACTT87600.020.199312-13
Produced by FastQC (version 0.11.5)