##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1793094 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765514245209676 33.0 31.0 34.0 30.0 34.0 2 31.96899827895247 33.0 31.0 34.0 30.0 34.0 3 32.04032025091824 34.0 31.0 34.0 30.0 34.0 4 35.57064381454625 37.0 35.0 37.0 33.0 37.0 5 35.57913249389045 37.0 35.0 37.0 33.0 37.0 6 35.601178744672616 37.0 35.0 37.0 33.0 37.0 7 35.54126108279878 37.0 35.0 37.0 33.0 37.0 8 35.50989184058393 37.0 35.0 37.0 33.0 37.0 9 37.13181350224807 39.0 37.0 39.0 34.0 39.0 10-11 37.04427291597652 39.0 37.0 39.0 33.0 39.0 12-13 36.94538825069962 39.0 37.0 39.0 33.0 39.0 14-15 38.09693775117199 40.0 38.0 41.0 33.0 41.0 16-17 37.946958162818014 40.0 38.0 41.0 33.0 41.0 18-19 38.11016767665276 40.0 38.0 41.0 33.0 41.0 20-21 38.098000997159104 40.0 38.0 41.0 33.0 41.0 22-23 38.005563010082014 40.0 38.0 41.0 33.0 41.0 24-25 37.89331959172247 40.0 38.0 41.0 33.0 41.0 26-27 37.71342773998463 40.0 38.0 41.0 32.0 41.0 28-29 37.502747485630984 40.0 37.0 41.0 32.0 41.0 30-31 37.265457081446925 40.0 37.0 41.0 31.5 41.0 32-33 36.99428278718238 40.0 36.0 41.0 31.0 41.0 34-35 36.75119876593196 39.0 36.0 41.0 30.0 41.0 36-37 36.482364560920956 39.0 35.5 41.0 30.0 41.0 38-39 36.18512944664363 39.0 35.0 40.5 30.0 41.0 40-41 35.96151596068025 39.0 35.0 40.0 29.0 41.0 42-43 35.76625514334441 39.0 35.0 40.0 27.5 41.0 44-45 35.472882905190694 39.0 35.0 40.0 26.5 41.0 46-47 35.052471314945 38.5 35.0 40.0 24.5 41.0 48-49 34.6389757034489 38.0 34.0 40.0 23.5 41.0 50-51 33.943224671991544 37.5 33.5 39.5 23.0 40.5 52-53 34.07361214749478 38.0 33.5 40.0 23.0 40.5 54-55 34.18921149699904 38.0 34.0 40.0 22.5 41.0 56-57 33.89565856558552 38.0 33.5 40.0 20.5 41.0 58-59 33.57500861639156 38.0 33.0 40.0 19.5 41.0 60-61 33.16128741716831 37.0 33.0 40.0 17.0 41.0 62-63 32.63422330340741 36.5 32.0 39.5 11.5 41.0 64-65 32.11948230265675 36.0 31.0 39.0 8.0 40.5 66-67 31.584766331268746 35.5 31.0 39.0 4.5 40.0 68-69 30.987215673021048 35.0 30.0 38.0 2.0 40.0 70-71 30.4411146320271 35.0 29.5 37.5 2.0 40.0 72-73 29.803698802182147 34.5 29.0 37.0 2.0 39.0 74-75 29.128610379600847 34.0 28.0 36.5 2.0 39.0 76-77 28.460606638581133 34.0 26.5 36.0 2.0 38.5 78-79 27.737210932611454 33.5 26.0 35.0 2.0 37.5 80-81 27.077181397071207 33.0 25.0 35.0 2.0 37.0 82-83 26.352084999447882 32.5 23.5 35.0 2.0 36.0 84-85 25.691759327731845 32.0 20.5 35.0 2.0 36.0 86-87 25.019055052328547 31.5 18.5 34.5 2.0 35.5 88-89 24.206232913611892 31.0 11.0 34.0 2.0 35.0 90-91 23.381935079811768 30.5 4.5 34.0 2.0 35.0 92-93 22.50426553209146 30.0 2.0 34.0 2.0 35.0 94-95 21.56164651713742 29.0 2.0 34.0 2.0 35.0 96-97 20.37732795938194 29.0 2.0 34.0 2.0 35.0 98-99 18.91688946591757 26.0 2.0 33.5 2.0 35.0 100-101 15.335076130978074 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.427639474617731 1101 2 0.37877448392372415 1101 3 0.3208620505469497 1101 4 0.12936005711008391 1101 5 0.24941817543244582 1101 6 0.18242129248367434 1101 7 0.25188724511158256 1101 8 0.20100620438892491 1101 9 0.3332299096231921 1101 10-11 0.38286048295825026 1101 12-13 0.45468577430448676 1101 14-15 0.6142990810756643 1101 16-17 0.685698412756885 1101 18-19 0.606282503098889 1101 20-21 0.659243822435819 1101 22-23 0.6590138367119138 1101 24-25 0.5542636225336395 1101 26-27 0.6350749896178272 1101 28-29 0.5689728154792846 1101 30-31 0.6791773107529906 1101 32-33 0.6888934527243791 1101 34-35 0.7140162475102372 1101 36-37 0.7089058738144729 1101 38-39 0.7901841842516077 1101 40-41 0.8056934120690471 1101 42-43 0.9350909996261123 1101 44-45 0.9473859236292412 1101 46-47 1.0173803921045703 1101 48-49 1.0639630223078669 1101 50-51 1.0762250661749633 1101 52-53 1.2262834136873977 1101 54-55 1.2608798995872519 1101 56-57 1.3443952517434283 1101 58-59 1.375486972759795 1101 60-61 1.3302732264480568 1101 62-63 1.462461687094489 1101 64-65 1.3242586892314065 1101 66-67 1.366879609347702 1101 68-69 1.6436273498329292 1101 70-71 1.8388248897926935 1101 72-73 1.846617341778316 1101 74-75 2.1315055277264854 1101 76-77 2.18345362030794 1101 78-79 2.2869539062594733 1101 80-81 2.339327896107573 1101 82-83 2.4647692663547893 1101 84-85 2.4980730504129376 1101 86-87 2.7504476191251435 1101 88-89 3.193088516419653 1101 90-91 3.2852432840370653 1101 92-93 3.6693571542675407 1101 94-95 3.8927754199000297 1101 96-97 4.4878583981344775 1101 98-99 4.713953944791037 1101 100-101 4.720062506839659 1102 1 0.337871237161405 1102 2 0.27464332847438655 1102 3 0.2001675457076857 1102 4 0.18967772075007616 1102 5 0.25401529797980515 1102 6 0.29840631232126213 1102 7 0.15965002999254096 1102 8 0.27398674208821205 1102 9 0.33627118671986267 1102 10-11 0.38436164534783757 1102 12-13 0.3157546558598554 1102 14-15 0.5201176209555385 1102 16-17 0.553539566704309 1102 18-19 0.4985546254870741 1102 20-21 0.5591592135077263 1102 22-23 0.6434548203927619 1102 24-25 0.5009485743170359 1102 26-27 0.43316228101225107 1102 28-29 0.407501788699939 1102 30-31 0.5401486242438622 1102 32-33 0.6445142159575852 1102 34-35 0.7237914064937527 1102 36-37 0.8430134593663041 1102 38-39 0.8563044323029629 1102 40-41 0.8987049917690442 1102 42-43 1.015222845729994 1102 44-45 0.907117654524157 1102 46-47 1.0260716279454982 1102 48-49 1.2782537661510602 1102 50-51 1.359777337750522 1102 52-53 1.3825910111496498 1102 54-55 1.3324896153439525 1102 56-57 1.290049839820803 1102 58-59 1.3759793075804367 1102 60-61 1.5049831721357876 1102 62-63 1.4555045784650815 1102 64-65 1.4244938638482765 1102 66-67 1.4368928418994038 1102 68-69 1.6631302882983796 1102 70-71 1.8528080226901302 1102 72-73 2.1191549092285626 1102 74-75 1.8700963377511641 1102 76-77 2.0899124389066266 1102 78-79 2.4162053738067613 1102 80-81 2.6304284900929567 1102 82-83 2.716741508334078 1102 84-85 2.539291344069216 1102 86-87 2.8263306662900334 1102 88-89 2.9334005319736143 1102 90-91 3.0769472644021008 1102 92-93 3.233655445122249 1102 94-95 3.5611518685797883 1102 96-97 3.724848417726747 1102 98-99 3.989197761953349 1102 100-101 3.8317998513627938 1103 1 0.6762195584248154 1103 2 0.6015331782413931 1103 3 0.5999022726090288 1103 4 0.450932374082349 1103 5 0.5123068508001012 1103 6 0.5865689424156173 1103 7 0.5689902957961834 1103 8 0.4929511159110618 1103 9 0.5787966750434279 1103 10-11 0.6799879551337611 1103 12-13 0.7643102438376772 1103 14-15 1.004769361589183 1103 16-17 1.0208780374933184 1103 18-19 0.947147130637795 1103 20-21 1.0734923611498886 1103 22-23 1.0338910620035051 1103 24-25 1.1264344662094103 1103 26-27 0.9578863969896503 1103 28-29 1.1219874683738311 1103 30-31 1.1604915339360602 1103 32-33 1.173107561139787 1103 34-35 1.2252227759219139 1103 36-37 1.3166753776445006 1103 38-39 1.3464957384192786 1103 40-41 1.4089869770729209 1103 42-43 1.5839179673577135 1103 44-45 1.7050253129585116 1103 46-47 1.737163801504849 1103 48-49 1.8376652135695153 1103 50-51 1.5562394788581244 1103 52-53 1.566113130504867 1103 54-55 1.7013355005160662 1103 56-57 1.7143561506678182 1103 58-59 1.8261081860941957 1103 60-61 1.8054507159721709 1103 62-63 1.7750901334606226 1103 64-65 1.8060646785019614 1103 66-67 1.9840774100790135 1103 68-69 2.119869109247883 1103 70-71 2.391892255741393 1103 72-73 2.3963217483227446 1103 74-75 2.587263928452458 1103 76-77 2.6498800755885874 1103 78-79 2.755327408274578 1103 80-81 3.0047007575589824 1103 82-83 3.113744364441107 1103 84-85 3.1392586270537173 1103 86-87 3.24782397698673 1103 88-89 3.8168220686122467 1103 90-91 3.972337260069306 1103 92-93 4.162194851441054 1103 94-95 4.2827913005416605 1103 96-97 4.701899420901178 1103 98-99 5.025328621172918 1103 100-101 4.6762045785294095 1104 1 0.3336729745738829 1104 2 0.32120191530143316 1104 3 0.3055886320871011 1104 4 0.26979943556821695 1104 5 0.30432620657072107 1104 6 0.3026650471871477 1104 7 0.2581279528812601 1104 8 0.19950415168553803 1104 9 0.2873984372852334 1104 10-11 0.34612137513472163 1104 12-13 0.3644402452821325 1104 14-15 0.6085779224250487 1104 16-17 0.5962921161682644 1104 18-19 0.5304696305174232 1104 20-21 0.4672258100400626 1104 22-23 0.49155994350959986 1104 24-25 0.4147644291349195 1104 26-27 0.5998323260184932 1104 28-29 0.583716487585157 1104 30-31 0.49774657751061824 1104 32-33 0.42942226093274627 1104 34-35 0.4899743287169329 1104 36-37 0.4863705964802705 1104 38-39 0.6136715774100736 1104 40-41 0.6655737169255787 1104 42-43 0.6118345738385287 1104 44-45 0.7346812906175302 1104 46-47 0.7154066153138814 1104 48-49 0.8107452848295651 1104 50-51 0.8239750462903572 1104 52-53 0.7273245513724973 1104 54-55 1.057955944446924 1104 56-57 1.195432514479272 1104 58-59 0.965981314314142 1104 60-61 1.0458534797065866 1104 62-63 1.1591240301335475 1104 64-65 1.2464915165779473 1104 66-67 1.374606564106653 1104 68-69 1.5087082444975302 1104 70-71 1.7801066618273858 1104 72-73 1.8457572050130047 1104 74-75 1.8177131556660946 1104 76-77 1.9026630694737072 1104 78-79 2.1160645646133567 1104 80-81 2.0737495803236783 1104 82-83 2.4809599260954975 1104 84-85 2.5654900296248577 1104 86-87 2.572507399395125 1104 88-89 2.8094083751267647 1104 90-91 2.961638622677036 1104 92-93 3.2043586532610746 1104 94-95 3.5379034869733594 1104 96-97 3.7855059980112955 1104 98-99 3.8702693982926633 1104 100-101 3.580380273074164 1105 1 0.2725805222840556 1105 2 0.24404551966499 1105 3 0.16702614642936453 1105 4 0.12127531906693889 1105 5 0.10873675230642732 1105 6 0.22232777158495765 1105 7 0.20129352635695597 1105 8 0.2399903628266955 1105 9 0.3062714969545084 1105 10-11 0.280185853724106 1105 12-13 0.24245938687140978 1105 14-15 0.2661480352922396 1105 16-17 0.41770116092340004 1105 18-19 0.36717926192043393 1105 20-21 0.3053490817780897 1105 22-23 0.2951403785699327 1105 24-25 0.20534766816501815 1105 26-27 0.284228303947593 1105 28-29 0.3040628877445144 1105 30-31 0.29817110858285645 1105 32-33 0.34020489000405973 1105 34-35 0.34164320840659457 1105 36-37 0.5085486591557498 1105 38-39 0.4866968857952827 1105 40-41 0.7178965736688241 1105 42-43 0.7320958740304064 1105 44-45 0.8500683395589377 1105 46-47 0.9582763521185456 1105 48-49 0.9887583110885529 1105 50-51 1.1220662155717775 1105 52-53 1.0946612402662694 1105 54-55 1.327510015773477 1105 56-57 1.3116218220967326 1105 58-59 1.3307295837059385 1105 60-61 1.3759222257066384 1105 62-63 1.3476153115495464 1105 64-65 1.5061663021024145 1105 66-67 1.6440987252825359 1105 68-69 1.730454207622703 1105 70-71 1.711906954515566 1105 72-73 1.82052738060775 1105 74-75 2.1137399468675255 1105 76-77 2.076684934504126 1105 78-79 2.192565227309295 1105 80-81 2.092623396840761 1105 82-83 2.1586730892531136 1105 84-85 2.06567046214219 1105 86-87 2.2802159548632908 1105 88-89 2.563470074570997 1105 90-91 2.5900855346471587 1105 92-93 2.932997380739554 1105 94-95 3.1535080539746794 1105 96-97 3.430194561193254 1105 98-99 3.599679656426204 1105 100-101 3.4643217487578557 1106 1 0.18332476189431546 1106 2 0.20217999460654923 1106 3 0.12036028429395884 1106 4 0.15422707050100826 1106 5 0.24746292360018174 1106 6 -0.004288030608940119 1106 7 0.05860056351715315 1106 8 0.1991596144680443 1106 9 0.13390785393860227 1106 10-11 0.18524206714953095 1106 12-13 0.3676585157351866 1106 14-15 0.31832473986334975 1106 16-17 0.24477719827450528 1106 18-19 0.3321442367798184 1106 20-21 0.34512277637939803 1106 22-23 0.31695905984484085 1106 24-25 0.18195528243378334 1106 26-27 0.20704346308692578 1106 28-29 0.2512271658483556 1106 30-31 0.18940743216611367 1106 32-33 0.2697776691510825 1106 34-35 0.3429861756575505 1106 36-37 0.3237316983778271 1106 38-39 0.039030562480533604 1106 40-41 0.3686129654818373 1106 42-43 0.3609024109157559 1106 44-45 0.363849428963114 1106 46-47 0.5587115071447357 1106 48-49 0.5099881994052566 1106 50-51 0.4406204669933018 1106 52-53 0.46039815205237034 1106 54-55 0.051624886314264984 1106 56-57 0.40423566449967296 1106 58-59 0.5265260357230801 1106 60-61 0.8133444957417524 1106 62-63 0.4495526188935628 1106 64-65 0.5111956060225538 1106 66-67 0.7476879280795963 1106 68-69 0.713310610299839 1106 70-71 0.779715957220386 1106 72-73 0.98662556430466 1106 74-75 1.2153844067993589 1106 76-77 1.390882914697908 1106 78-79 1.6581285845887415 1106 80-81 1.6408663566266917 1106 82-83 1.516689451530592 1106 84-85 1.7961151151615269 1106 86-87 1.6173876301304766 1106 88-89 1.8416687723515786 1106 90-91 2.212012444063923 1106 92-93 2.5646198262175623 1106 94-95 2.620031537681591 1106 96-97 2.890479436074383 1106 98-99 3.0034797978781427 1106 100-101 3.217731245039653 1107 1 0.2796489527994659 1107 2 0.23349247389508676 1107 3 0.08953087204816512 1107 4 0.12491647537203221 1107 5 0.19051110210916278 1107 6 0.23505088917165295 1107 7 0.15838178253839175 1107 8 0.19822687341773104 1107 9 0.2873847905294724 1107 10-11 0.3103902487431185 1107 12-13 0.16916649426516273 1107 14-15 0.24083139087868233 1107 16-17 0.24012311474493941 1107 18-19 0.16011860111650122 1107 20-21 0.337022984406687 1107 22-23 0.4185135056322338 1107 24-25 0.37872218574764105 1107 26-27 0.4416344875484626 1107 28-29 0.5595348719138045 1107 30-31 0.6126692468625023 1107 32-33 0.4462587280838477 1107 34-35 0.4746860424532926 1107 36-37 0.503297509682838 1107 38-39 0.4589918435392377 1107 40-41 0.5078948907091743 1107 42-43 0.6056958565987287 1107 44-45 0.5954763970651271 1107 46-47 0.7352667099352601 1107 48-49 0.8600754033356637 1107 50-51 0.5457229227680571 1107 52-53 0.505481437960988 1107 54-55 0.7227254217064925 1107 56-57 0.7562960289195715 1107 58-59 0.812053748970861 1107 60-61 0.9696643681916797 1107 62-63 1.1244988716714843 1107 64-65 1.1994215331108435 1107 66-67 1.2494160176498958 1107 68-69 1.2872224147660738 1107 70-71 1.336376155637275 1107 72-73 1.3495833778645512 1107 74-75 1.6021397102783794 1107 76-77 1.6494058501947286 1107 78-79 1.8515788237309856 1107 80-81 1.8619108454882713 1107 82-83 1.986643219156381 1107 84-85 2.0985337613795814 1107 86-87 2.1733895895658613 1107 88-89 2.469609092771904 1107 90-91 2.562830332882662 1107 92-93 2.827091912611568 1107 94-95 2.999874624375284 1107 96-97 3.2185142191653924 1107 98-99 3.2665833805263276 1107 100-101 3.3086952878155653 1108 1 0.2737233337533205 1108 2 0.16045985798083606 1108 3 0.1396547913729762 1108 4 0.189401281152918 1108 5 0.13694565892325272 1108 6 0.06473514702715022 1108 7 0.1304233428807322 1108 8 0.07941628146203783 1108 9 0.23242099041296171 1108 10-11 0.07042510436629357 1108 12-13 0.059536425459150166 1108 14-15 0.24550571969317758 1108 16-17 0.3374607453617742 1108 18-19 0.18146148590184197 1108 20-21 0.1871545425008918 1108 22-23 0.1532923061714655 1108 24-25 0.23233468484037445 1108 26-27 0.17107919794531057 1108 28-29 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2316 44-45 -0.7880954166719505 2316 46-47 -0.599419116333074 2316 48-49 -0.49601624678572165 2316 50-51 -0.8997699869139453 2316 52-53 -1.091451621805156 2316 54-55 -1.1605227044069508 2316 56-57 -0.8517040294827822 2316 58-59 -0.868949128334954 2316 60-61 -0.9924589830244841 2316 62-63 -1.1461897292036127 2316 64-65 -1.0620145015870825 2316 66-67 -1.283506082271085 2316 68-69 -1.247925037567338 2316 70-71 -1.3685845551688693 2316 72-73 -1.310643356901899 2316 74-75 -1.4869623609512104 2316 76-77 -1.4406076442136175 2316 78-79 -2.129131695349635 2316 80-81 -1.9666750281206191 2316 82-83 -1.96272354836983 2316 84-85 -1.8894765570295604 2316 86-87 -1.642311512135155 2316 88-89 -1.8252228185713442 2316 90-91 -1.9391369108207073 2316 92-93 -2.305225178515652 2316 94-95 -2.379295854323569 2316 96-97 -2.719595370872355 2316 98-99 -2.9535277918192655 2316 100-101 -2.907715748942529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5447.0 3 1767.0 4 1770.0 5 1937.0 6 2326.0 7 2852.0 8 3637.0 9 4439.0 10 5510.0 11 6922.0 12 8887.0 13 10721.0 14 12594.0 15 15276.0 16 18485.0 17 23086.0 18 22105.0 19 17795.0 20 17603.0 21 18861.0 22 20576.0 23 22610.0 24 25507.0 25 28373.0 26 32357.0 27 36957.0 28 42479.0 29 49754.0 30 57992.0 31 69498.0 32 84932.0 33 106192.0 34 139019.0 35 189538.0 36 244825.0 37 278155.0 38 152692.0 39 9618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.637088925953584 19.012004816045117 14.893154503949033 22.457751754052264 2 15.29725317611798 20.024091128963416 40.81532682311209 23.863328871806516 3 19.049999832601056 24.760256812300028 29.97032574718519 26.21941760791373 4 12.438059971340182 16.97636680996576 37.5070310302803 33.078542188413756 5 13.171054357503076 37.836472581234844 34.4019893221848 14.590483739077287 6 31.416047996972562 36.67348919660663 17.3370588402741 14.573403966146707 7 28.02712738208488 33.48312349877391 20.58735078405499 17.902398335086218 8 26.34392344412184 34.90328973355859 19.597302780015667 19.155484042303907 9 26.584120409219945 16.758578978378473 20.455250495456088 36.202050116945486 10-11 24.8684729583527 26.911361738003826 26.87595865902399 21.344206644619483 12-13 25.947428414566986 24.536670499732466 27.431527886809064 22.08437319889148 14-15 22.717040248914735 25.675329624011322 26.32035107823441 25.28727904883953 16-17 21.559642880555234 28.949772581137832 27.195613696180676 22.294970842126258 18-19 21.50487687705784 27.12239424226649 30.02530920923786 21.34741967143781 20-21 22.235688894208597 26.158898731277674 30.460523911201438 21.144888463312295 22-23 21.910270345067705 26.23106546711691 30.27332459932553 21.58533958848986 24-25 21.608838176959978 26.39061268770495 30.529106405016325 21.471442730318742 26-27 21.247669244970687 26.730743422618374 30.31896921408461 21.702618118326324 28-29 21.369090527659555 27.02545120995909 30.19401972446454 21.411438537916812 30-31 21.405595533214935 26.615440582820156 30.182770158867257 21.796193725097655 32-33 21.203368131857403 26.668896614041582 30.10071866982058 22.027016584280435 34-35 21.898742900059453 26.837363758840137 29.872409614991348 21.391483726109062 36-37 21.96917663954846 26.34949843132507 29.934602742767996 21.74672218635847 38-39 21.539190589059583 26.774913158659565 29.812449480848606 21.873446771432253 40-41 21.953056949122853 26.643959275077865 29.879316312644633 21.523667463154645 42-43 21.643253875061987 26.574778223231014 29.675692440540175 22.10627546116682 44-45 21.663130334479018 26.874869618667375 29.707449389731693 21.754550657121914 46-47 22.181332043591272 27.10143023580468 29.025946296635652 21.691291423968394 48-49 21.834129709396883 27.230761807085184 28.896192373143027 22.038916110374906 50-51 21.956090607709537 27.376310480975206 28.80309659915702 21.864502312158233 52-53 22.24057335866794 27.244409527084517 28.66653893383244 21.8484781804151 54-55 21.873555854963143 27.442499844516917 28.79368272916185 21.890261571358092 56-57 21.955777702662754 27.552758078676664 28.557244899894357 21.934219318766225 58-59 21.961429470637597 27.096506642652702 28.66214629202572 22.279917594683983 60-61 21.71061181847643 27.3784403528999 28.897653562605598 22.013294266018075 62-63 21.72902715518311 27.35180418660651 28.9988752158997 21.92029344231068 64-65 21.628059591099767 27.36656301970482 28.843622867109797 22.161754522085616 66-67 21.552160721058385 27.46996519643933 28.646453829507173 22.331420252995112 68-69 21.983496934260483 27.102442496119373 28.779493763940568 22.134566805679583 70-71 21.845497021516092 27.159188174530595 28.525338797489592 22.469976006463725 72-73 21.826512060253997 27.25025874179117 28.512243764748142 22.41098543320669 74-75 21.806713619501323 27.600547281080594 28.290728293238242 22.302010806179837 76-77 21.93867562781488 27.195727651742263 28.513769399166165 22.351827321276684 78-79 21.963997314872476 27.369810498826304 28.3477290341545 22.318463152146723 80-81 22.09669691210898 27.405416289923394 28.072649303287584 22.425237494680044 82-83 22.07021129317716 27.466662799382203 28.137450726984405 22.32567518045623 84-85 22.046912287737474 27.530507879464977 28.052468225984622 22.370111606812927 86-87 22.14614966327854 27.437235955996155 27.915450147097783 22.50116423362753 88-89 22.18807232229175 27.40449975370791 27.800435906318633 22.60699201768171 90-91 22.10498187187974 27.458621080167074 27.85142026388563 22.58497678406756 92-93 22.300619143239626 27.071815037929497 27.999776885319054 22.627788933511823 94-95 22.04504399965635 26.914294672175505 28.1396128565802 22.90104847158795 96-97 22.349367362586882 26.610489873868588 28.061108509386706 22.979034254157824 98-99 22.171791984706744 26.738599715343263 27.73355951169349 23.3560487882565 100-101 22.907768244238568 25.72567143964199 27.95758079267228 23.408979523447154 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1564.0 1 979.0 2 552.5 3 725.0 4 601.0 5 587.0 6 1142.5 7 2517.5 8 3976.0 9 4490.5 10 3586.5 11 2435.0 12 2265.0 13 2333.5 14 2404.5 15 2519.5 16 2606.5 17 2751.5 18 2990.0 19 3365.0 20 3816.5 21 4461.5 22 5358.0 23 6480.0 24 7782.0 25 9646.5 26 12246.5 27 15248.5 28 17381.0 29 19399.5 30 22137.5 31 25831.5 32 31393.5 33 35049.5 34 37039.0 35 40098.5 36 44221.5 37 48015.0 38 50466.0 39 52380.0 40 53841.5 41 55701.5 42 56582.0 43 57128.5 44 59019.0 45 61823.5 46 62715.5 47 63622.5 48 65113.5 49 63609.0 50 61346.5 51 59080.5 52 57407.5 53 56795.0 54 56559.0 55 54013.0 56 50119.0 57 46547.5 58 42821.5 59 37876.5 60 31271.5 61 25907.0 62 21378.5 63 16099.5 64 11478.0 65 8559.5 66 6384.5 67 4834.0 68 3692.0 69 2791.5 70 2116.0 71 1475.5 72 1017.0 73 742.5 74 507.0 75 359.5 76 257.0 77 154.5 78 97.0 79 60.5 80 37.0 81 22.0 82 14.0 83 9.0 84 7.5 85 8.0 86 7.0 87 5.5 88 4.5 89 3.5 90 2.5 91 1.0 92 1.5 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4588158791452093 2 0.040879061555055114 3 0.053984899843510716 4 0.05788876656773153 5 0.08527160316190897 6 0.08259466597958612 7 0.04511754542706629 8 0.04071175298115994 9 0.044950236853171115 10-11 0.04890987310202365 12-13 0.04433677208222213 14-15 0.03753289007715156 16-17 0.03884347390599712 18-19 0.04801756070791604 20-21 0.0566339522635177 22-23 0.04829640833107467 24-25 0.06614265621322697 26-27 0.05713587798520323 28-29 0.044727158754644204 30-31 0.04701370926454497 32-33 0.04598197305885804 34-35 0.05094546075108165 36-37 0.0455637016241201 38-39 0.025486672756698758 40-41 0.02412031940322147 42-43 0.023032813672902816 44-45 0.015392388798356362 46-47 0.0271876432579664 48-49 0.02757802993038848 50-51 0.016089507856252936 52-53 0.01614527738088466 54-55 0.01642412500404329 56-57 0.01606162309393707 58-59 0.013858726870983896 60-61 0.01622893166783225 62-63 0.017037589774992274 64-65 0.01726066787351918 66-67 0.009703897285920313 68-69 0.011990447795821079 70-71 0.01781836311983644 72-73 0.015810660233094305 74-75 0.012882760189928693 76-77 0.009425049662761685 78-79 0.014249113543405979 80-81 0.017678939308257126 82-83 0.015448158322988086 84-85 0.013245262100034912 86-87 0.0038480971995890905 88-89 0.02827514898828505 90-91 0.03206747666324242 92-93 0.01639624024172743 94-95 0.03234632428640105 96-97 0.026741487060912592 98-99 0.02278185081206005 100-101 0.14675750406838683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1793094.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.13756908924553 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19343621304901 43.33451858646306 2 13.062164995704062 14.665563798029224 3 4.2807701426971985 7.2093608892253025 4 1.8720575540288664 4.203710411133579 5 0.9579286494760737 2.688789287126537 6 0.6031759875209072 2.03165002034573 7 0.3754511892930124 1.475384195500312 8 0.26006394933926646 1.1679486338924094 9 0.19032224957268243 0.9615805591146381 >10 1.076158510816635 11.209786735037017 >50 0.08366710096216151 3.228165148345262 >100 0.039914592970034024 4.033401424705842 >500 0.00319272788239842 1.2610090139307595 >1k 0.0013968184485493086 1.1403074759978826 >5k 1.9954549264990126E-4 0.8092323990970778 >10k+ 9.977274632495063E-5 0.5795914220554098 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10348 0.5771030408891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8883 0.4954006873036216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5565 0.3103574045755549 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 0.12035063415526458 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1870 0.10428901106132751 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.576952463172594E-5 0.0 0.0 2.788476231586297E-5 0.0 12-13 1.1153904926345188E-4 0.0 0.0 5.576952463172594E-5 0.0 14-15 1.1153904926345188E-4 0.0 0.0 5.576952463172594E-5 0.0 16-17 1.6730857389517784E-4 0.0 0.0 5.576952463172594E-5 0.0 18-19 1.6730857389517784E-4 0.0 0.0 5.576952463172594E-5 0.0 20-21 2.788476231586297E-4 0.0 0.0 8.365428694758891E-5 0.0 22-23 2.788476231586297E-4 0.0 0.0 2.509628608427667E-4 0.0 24-25 2.788476231586297E-4 0.0 0.0 5.855800086331225E-4 0.0 26-27 2.788476231586297E-4 0.0 0.0 0.0013384685911614225 0.0 28-29 2.788476231586297E-4 0.0 0.0 0.0047682943560125686 0.0 30-31 2.788476231586297E-4 0.0 0.0 0.014834693552039101 0.0 32-33 2.788476231586297E-4 0.0 0.0 0.034075179549984555 0.0 34-35 2.788476231586297E-4 0.0 0.0 0.06458110952353864 0.0 36-37 2.788476231586297E-4 0.0 0.0 0.112738094043034 0.0 38-39 2.788476231586297E-4 0.0 0.0 0.1871625246640723 0.0 40-41 2.788476231586297E-4 0.0 0.0 0.2636504276964844 0.0 42-43 2.788476231586297E-4 0.0 0.0 0.3386604383261558 0.0 44-45 3.067323854744927E-4 0.0 0.0 0.4326599720929299 0.0 46-47 3.346171477903557E-4 0.0 0.0 0.5460673004315446 0.0 48-49 3.346171477903557E-4 0.0 0.0 0.6588890487615262 0.0 50-51 3.346171477903557E-4 0.0 0.0 0.7840079772728034 0.0 52-53 3.346171477903557E-4 0.0 0.0 0.9007335923270057 0.0 54-55 3.346171477903557E-4 0.0 0.0 1.0231198141313282 0.0 56-57 3.346171477903557E-4 0.0 0.0 1.1613724656933768 0.0 58-59 3.903866724220816E-4 0.0 0.0 1.3138184612741997 5.576952463172594E-5 60-61 3.903866724220816E-4 0.0 0.0 1.475745276042416 5.576952463172594E-5 62-63 4.1827143473794454E-4 0.0 0.0 1.633489376463253 5.576952463172594E-5 64-65 4.4615619705380753E-4 0.0 0.0 1.7922652130897765 5.576952463172594E-5 66-67 4.4615619705380753E-4 0.0 0.0 1.9766392615222625 5.576952463172594E-5 68-69 4.4615619705380753E-4 0.0 0.0 2.177772052106582 5.576952463172594E-5 70-71 4.4615619705380753E-4 0.0 0.0 2.390839520962091 5.576952463172594E-5 72-73 4.4615619705380753E-4 0.0 0.0 2.5999194688064318 5.576952463172594E-5 74-75 4.4615619705380753E-4 0.0 0.0 2.812819629088046 5.576952463172594E-5 76-77 4.4615619705380753E-4 0.0 0.0 3.039383322904432 5.576952463172594E-5 78-79 4.4615619705380753E-4 0.0 0.0 3.2924654256832047 5.576952463172594E-5 80-81 5.576952463172594E-4 0.0 0.0 3.552936990475681 5.576952463172594E-5 82-83 5.576952463172594E-4 0.0 0.0 3.8167826115083763 5.576952463172594E-5 84-85 5.576952463172594E-4 0.0 0.0 4.083918634494344 5.576952463172594E-5 86-87 5.576952463172594E-4 0.0 0.0 4.361121056676337 8.365428694758891E-5 88-89 5.576952463172594E-4 0.0 0.0 4.650230272367204 1.1153904926345188E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5905 0.0 44.04603 1 GTATCAA 9515 0.0 35.861416 1 ATCAACG 11595 0.0 29.34937 3 TATCAAC 11605 0.0 29.28149 2 TAAGCGA 65 0.0058276667 29.248472 5 TCAACGC 11785 0.0 28.835867 4 CAACGCA 12020 0.0 28.311644 5 AACGCAG 12335 0.0 27.54704 6 CGCAGAG 13765 0.0 24.537582 8 ACGCAGA 13925 0.0 24.289757 7 GTACATG 8180 0.0 23.21988 1 GAGTACT 8350 0.0 23.011566 12-13 TACATGG 7995 0.0 22.826801 2 GCAGAGT 14840 0.0 22.215902 9 GTATACG 110 0.0028007235 21.692394 1 GTACTTT 8815 0.0 21.421364 14-15 GTATAGG 535 0.0 21.408567 1 ACATGGG 8165 0.0 21.246786 3 CAGAGTA 14245 0.0 20.307236 10-11 AGTACTT 8760 0.0 20.1993 12-13 >>END_MODULE