FastQCFastQC Report
Thu 26 May 2016
SRR938070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793094
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105050.585858856256281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.5090084513137627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.3252478676522257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.12246987609127018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.045.091741
GTATCAA93600.036.1865731
ATCAACG115100.029.0953333
TCAACGC116600.028.8815774
TATCAAC116650.028.8324992
CAACGCA118950.028.3109875
AACGCAG121200.027.941396
ACGCAGA137100.024.6662797
CGCAGAG137200.024.5790658
GAGTACT82750.023.73387512-13
GTACTTT86900.022.35448314-15
GCAGAGT149750.022.2971639
AGTACTT85700.020.97713512-13
AGAGTAC130200.020.55625710-11
GTGTAAG8500.020.1195071
CAGAGTA144600.020.02011310-11
TATCGCG600.004765189719.79184290-91
TAAGGTG10200.019.5579055
ACTTTTT100350.019.40562216-17
CCTATAC4250.019.0006983
Produced by FastQC (version 0.11.5)