FastQCFastQC Report
Thu 26 May 2016
SRR938069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827051
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110540.6050186885861424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.5039268197767878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.31717779087721143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.11833276684668353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56200.046.231841
GTATCAA90400.037.459021
ATCAACG112050.030.1978153
TATCAAC112900.029.9283682
TCAACGC114050.029.7099274
CAACGCA116600.029.1009435
AACGCAG120000.028.4348286
ACGCAGA132100.025.7301047
CGCAGAG132750.025.4608768
GTACATG83000.024.859031
TACATGG83100.023.9618512
TAAGGTG12100.023.958075
GAGTACT83200.023.6808812-13
GTAAGGT11050.023.6541714
ACATGGG82850.023.2311083
GCAGAGT146000.022.4658379
GTACTTT87900.022.37475214-15
CGCGCGT1604.960929E-520.797757
CAGAGTA138200.020.74071710-11
TGGTATC30300.020.7032572
Produced by FastQC (version 0.11.5)