FastQCFastQC Report
Thu 26 May 2016
SRR938069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827051
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108250.5924848293780525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92540.5064992712299766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58450.3199144413593271No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22410.12265667460842637No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18390.100654004732216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59900.045.4452861
GTATCAA96850.036.4460331
GTAAGGT9850.029.4143734
TAAGGTG10550.029.263545
ATCAACG120450.029.1811753
TATCAAC121900.028.9565052
TCAACGC122200.028.8013574
CAACGCA123550.028.4097635
AACGCAG126150.027.824236
GCGTAAA1109.4696254E-525.9131151
TATCGCG559.486277E-525.9074490-91
CGCAGAG140650.024.7869038
ACGCAGA141000.024.7590627
AGGTAAG11300.023.9638982
GGTAAGG11500.023.9556523
GAGTACT87250.023.92541112-13
GTCCGCG1000.001654630823.748498
GTACTTT90300.022.77540614-15
GTACATG79900.022.118581
GCAGAGT156100.022.0597749
Produced by FastQC (version 0.11.5)