FastQCFastQC Report
Thu 26 May 2016
SRR938068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765256
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101300.5738544437747273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84070.47624820422646913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.29939000348957884No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.11669695500256053No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18370.10406422637849694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56050.048.5092471
GTATCAA90400.036.608371
ATCAACG114150.029.0329783
TATCAAC115100.028.6281072
TCAACGC115600.028.6276784
CAACGCA117450.028.1362695
AACGCAG120250.027.5151926
GAGTACT81450.025.12284712-13
ACGCAGA133900.024.6044337
CGCAGAG134850.024.3585178
GTACATG79900.023.5404281
TACATGG78500.023.5015662
GTACTTT86750.023.45031714-15
GCAGAGT143950.022.1582079
GTGTAAG8250.021.9327811
ACATGGG79550.021.8763873
AGTACTT85650.021.14386612-13
CAGAGTA137750.021.01447910-11
AGAGTAC126000.020.61638610-11
GGTAAGG10000.020.4457553
Produced by FastQC (version 0.11.5)