FastQCFastQC Report
Thu 26 May 2016
SRR938068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765256
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101880.5771400861971295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.5095011715014707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54880.3108897519679865No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.1237780809129101No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18610.10542380255328404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.041.8431
GTATCAA91000.035.4975781
ACCGTCG1503.346031E-828.4935828
ATCAACG113250.028.1411363
TATCAAC114700.027.7885362
TCAACGC114750.027.7709184
CAACGCA116300.027.3191285
AACGCAG118800.026.783456
CCGTCGT1658.433926E-825.903269
ACGCAGA134300.023.6562527
CGCAGAG134600.023.638818
GTACATG77850.022.8214991
TACATGG76000.022.7506772
GAGTACT80500.022.71420712-13
ATACCGT2354.663889E-922.2296686
TACCGTC1954.2332613E-721.9181427
GCAGAGT145650.021.4867529
GTACTTT85250.021.19790814-15
ACATGGG77850.021.1093483
GTGTAGG6350.020.9466931
Produced by FastQC (version 0.11.5)