FastQCFastQC Report
Thu 26 May 2016
SRR938067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024950
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104220.5146793747993778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.42025729030346426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.27106842144250476No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.10039754068001679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62250.040.3995481
GTATCAA101950.036.416451
TATCAAC126100.029.1677342
ATCAACG126450.028.9742623
TCAACGC127400.028.7209054
CAACGCA129900.028.1315735
AACGCAG132200.027.6373626
GTACATG91050.025.681561
ACGCAGA147550.024.5015457
TACATGG93550.024.2806852
CGCAGAG149600.024.0387748
GAGTACT78550.023.49478112-13
GCAGAGT154950.022.840319
ACATGGG99950.022.298053
GTACTTT82500.021.91005114-15
GTAAGGT6100.021.8124774
AGTACTT81350.021.57662412-13
AGAGTAC129400.021.29245210-11
GTAGCCC7950.019.7253023
CATGGGG52400.019.67914
Produced by FastQC (version 0.11.5)