##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938067_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2024950 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7439062692906 33.0 31.0 34.0 30.0 34.0 2 31.90772710437295 33.0 31.0 34.0 30.0 34.0 3 31.987342403516138 34.0 31.0 34.0 30.0 34.0 4 35.52715326304353 37.0 35.0 37.0 33.0 37.0 5 35.53659152077829 37.0 35.0 37.0 33.0 37.0 6 35.56492357835996 37.0 35.0 37.0 33.0 37.0 7 35.48947677720437 37.0 35.0 37.0 33.0 37.0 8 35.45884540359021 37.0 35.0 37.0 33.0 37.0 9 37.06780908170572 39.0 37.0 39.0 33.0 39.0 10-11 36.94636558927381 39.0 37.0 39.0 33.0 39.0 12-13 36.803983555149514 39.0 37.0 39.0 33.0 39.0 14-15 37.912917602903775 40.0 38.0 41.0 33.0 41.0 16-17 37.737358947134496 40.0 38.0 41.0 32.5 41.0 18-19 37.92748240697301 40.0 38.0 41.0 33.0 41.0 20-21 37.91653398849354 40.0 38.0 41.0 33.0 41.0 22-23 37.794097878959974 40.0 38.0 41.0 32.0 41.0 24-25 37.64884836662634 40.0 37.5 41.0 32.0 41.0 26-27 37.43556359416282 40.0 37.0 41.0 32.0 41.0 28-29 37.209849872836365 40.0 37.0 41.0 31.0 41.0 30-31 36.95354379120472 39.5 36.5 41.0 30.5 41.0 32-33 36.630799772833896 39.0 35.5 41.0 30.0 41.0 34-35 36.32136571273365 39.0 35.0 40.5 30.0 41.0 36-37 36.00639176275958 39.0 35.0 40.0 29.5 41.0 38-39 35.67562013876886 38.0 35.0 40.0 27.5 41.0 40-41 35.499942714634926 38.0 35.0 40.0 27.0 41.0 42-43 35.22115607792785 38.0 34.5 40.0 25.5 41.0 44-45 34.82370453591447 38.0 34.0 40.0 24.5 41.0 46-47 34.32390034321835 38.0 33.5 40.0 23.5 41.0 48-49 33.82261241018297 38.0 33.0 40.0 20.0 41.0 50-51 32.98846909800242 37.5 32.0 39.5 17.5 40.5 52-53 32.987383886021874 37.5 32.5 39.5 16.0 40.5 54-55 32.96371416578187 37.5 32.0 40.0 13.0 41.0 56-57 32.47642287463888 37.0 31.5 40.0 8.0 41.0 58-59 32.001034593446754 36.5 31.0 40.0 4.5 41.0 60-61 31.425049507395244 36.0 30.5 39.0 2.0 41.0 62-63 30.782675621620285 35.0 29.0 39.0 2.0 40.5 64-65 30.155323588236747 35.0 28.0 39.0 2.0 40.0 66-67 29.44689572582039 34.5 27.5 38.0 2.0 40.0 68-69 28.72357860687918 34.0 26.0 37.5 2.0 40.0 70-71 27.884525790760264 34.0 25.5 37.0 2.0 39.0 72-73 27.066374478382183 33.5 22.5 36.0 2.0 39.0 74-75 26.22429516778192 32.5 20.0 35.5 2.0 38.5 76-77 25.363021556087805 32.0 15.5 35.0 2.0 37.5 78-79 24.515981382256353 31.0 7.5 35.0 2.0 37.0 80-81 23.694121830168648 31.0 2.0 35.0 2.0 36.0 82-83 22.848729598261684 30.0 2.0 34.0 2.0 36.0 84-85 22.029717030050126 29.5 2.0 34.0 2.0 35.0 86-87 21.246495715943603 29.0 2.0 34.0 2.0 35.0 88-89 20.416067557223634 28.0 2.0 34.0 2.0 35.0 90-91 19.553495394948023 26.5 2.0 34.0 2.0 35.0 92-93 18.62574557396479 25.0 2.0 33.5 2.0 35.0 94-95 17.67416109039729 22.0 2.0 33.0 2.0 35.0 96-97 16.552686979925433 10.0 2.0 32.5 2.0 34.5 98-99 15.254782834144052 2.0 2.0 32.0 2.0 34.0 100-101 12.345935702116101 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44251102444080104 1101 2 0.3481176963426371 1101 3 0.4129232598153152 1101 4 0.24379975765623385 1101 5 0.34376878504503594 1101 6 0.36638591449867874 1101 7 0.3702472206680767 1101 8 0.42824011651328675 1101 9 0.4527878682487483 1101 10-11 0.38426615308361534 1101 12-13 0.4344621840051204 1101 14-15 0.6874390445797474 1101 16-17 0.6539507047090112 1101 18-19 0.740978110907669 1101 20-21 0.6627924427371639 1101 22-23 0.8284034649904299 1101 24-25 0.6923305729641598 1101 26-27 0.7630917018040719 1101 28-29 0.77654855341504 1101 30-31 0.866443422114159 1101 32-33 0.9870009831241049 1101 34-35 0.9615002547448697 1101 36-37 1.1410301716861753 1101 38-39 1.2543471012722307 1101 40-41 1.4741357622315547 1101 42-43 1.7691538174376475 1101 44-45 1.8892647135843248 1101 46-47 1.9800439693656315 1101 48-49 2.0971062661632374 1101 50-51 2.3927499788945994 1101 52-53 2.4158777826446496 1101 54-55 2.3778396490437856 1101 56-57 2.608300628060185 1101 58-59 2.840659496503207 1101 60-61 3.043891520105671 1101 62-63 3.008442534834014 1101 64-65 3.1396613283010915 1101 66-67 3.2068491060932303 1101 68-69 3.4529394272047966 1101 70-71 3.7772168770535295 1101 72-73 4.183451204155741 1101 74-75 4.355017195293055 1101 76-77 4.550312442622676 1101 78-79 4.851950054317893 1101 80-81 5.058244332664657 1101 82-83 5.264905876586933 1101 84-85 5.3177341587048055 1101 86-87 5.472349348151393 1101 88-89 5.560949692327441 1101 90-91 5.70818641869155 1101 92-93 6.008647952310245 1101 94-95 6.18036225540078 1101 96-97 5.986750350465254 1101 98-99 5.7965697854298615 1101 100-101 5.008074274170619 1102 1 0.4814724393248717 1102 2 0.5131881478692186 1102 3 0.439534671643969 1102 4 0.4332106407366041 1102 5 0.4187166017070396 1102 6 0.38676105561078344 1102 7 0.4425760609275642 1102 8 0.4749651899237435 1102 9 0.6270458034931679 1102 10-11 0.6148187336260662 1102 12-13 0.7050614963750661 1102 14-15 0.8903416925022185 1102 16-17 0.9473691716606325 1102 18-19 0.8396327617376187 1102 20-21 0.7159658972580516 1102 22-23 0.7688374309562747 1102 24-25 0.7949509966703445 1102 26-27 0.8652060516591789 1102 28-29 0.8286797884796755 1102 30-31 0.9279361389604546 1102 32-33 1.0144769176802484 1102 34-35 1.1326939264899707 1102 36-37 1.2458812119758917 1102 38-39 1.3889878322570723 1102 40-41 1.3505725327502986 1102 42-43 1.4841342145134533 1102 44-45 1.6141372704553234 1102 46-47 1.8465770016598029 1102 48-49 1.980341826092868 1102 50-51 2.090055421078034 1102 52-53 2.1380075382433006 1102 54-55 2.190319909429796 1102 56-57 2.458092698765789 1102 58-59 2.3660485799740343 1102 60-61 2.600436227316134 1102 62-63 2.9319161922990453 1102 64-65 2.899159982308678 1102 66-67 3.106444825933835 1102 68-69 3.3461218228461576 1102 70-71 3.6932097434545383 1102 72-73 3.7383395527179246 1102 74-75 4.0092878668097995 1102 76-77 4.391525379587776 1102 78-79 4.44927993985225 1102 80-81 4.524858735587571 1102 82-83 4.75446277652679 1102 84-85 5.03350832143963 1102 86-87 5.267524680950835 1102 88-89 5.318759342948336 1102 90-91 5.439611080102896 1102 92-93 5.541001943491338 1102 94-95 5.3410972283683655 1102 96-97 5.4680516694413335 1102 98-99 5.190741975972218 1102 100-101 4.647352005313055 1103 1 0.5994746983840038 1103 2 0.35604921573257897 1103 3 0.31825372406024854 1103 4 0.27843456234114683 1103 5 0.26619958244398134 1103 6 0.2239470005996438 1103 7 0.2977612121929383 1103 8 0.42449048222768226 1103 9 0.3691680979645824 1103 10-11 0.47579196072256025 1103 12-13 0.5157248660365141 1103 14-15 0.6978545905087543 1103 16-17 0.7693324161579937 1103 18-19 0.5810651273388672 1103 20-21 0.6958709557188385 1103 22-23 0.7281255220513074 1103 24-25 0.7570766492026308 1103 26-27 0.7496673881674099 1103 28-29 0.7417334869258099 1103 30-31 0.788659037882816 1103 32-33 0.9651576882025807 1103 34-35 0.9914477806280146 1103 36-37 0.9891284568395875 1103 38-39 1.0782634069433996 1103 40-41 1.1703408265500954 1103 42-43 1.3092021389265582 1103 44-45 1.3346718126905728 1103 46-47 1.519359918288309 1103 48-49 1.6975106754991032 1103 50-51 1.6287891268508474 1103 52-53 1.7724139246645265 1103 54-55 1.9788612898980915 1103 56-57 2.280806927785541 1103 58-59 2.3161004772784253 1103 60-61 2.592179976743733 1103 62-63 2.645499548528246 1103 64-65 2.598561636916852 1103 66-67 2.8588565241967956 1103 68-69 3.0693748679968174 1103 70-71 3.103058020158752 1103 72-73 3.40263115451296 1103 74-75 3.6903758785324854 1103 76-77 4.011295199778882 1103 78-79 4.072792471524874 1103 80-81 4.353025234351193 1103 82-83 4.677598289546907 1103 84-85 4.66591360885911 1103 86-87 4.972925053390316 1103 88-89 5.007233562199179 1103 90-91 4.993486587628006 1103 92-93 5.201770634152879 1103 94-95 5.152911497074289 1103 96-97 5.0054711522999575 1103 98-99 5.046882648194947 1103 100-101 4.2405473528723565 1104 1 0.6389244053526788 1104 2 0.504252578310787 1104 3 0.43176287619186127 1104 4 0.35998757013589966 1104 5 0.38349367404686063 1104 6 0.36770887040751177 1104 7 0.36809630487939415 1104 8 0.3622291891613969 1104 9 0.4547804344088249 1104 10-11 0.5533310801138924 1104 12-13 0.6084756903691826 1104 14-15 0.7093766057254101 1104 16-17 0.8468837694617335 1104 18-19 0.7856965370351148 1104 20-21 0.7225551576599543 1104 22-23 0.7919285793642814 1104 24-25 0.7064160179327459 1104 26-27 0.871582985823153 1104 28-29 0.9209824412175749 1104 30-31 1.0593669736139972 1104 32-33 1.1636392266461968 1104 34-35 1.1830494910454803 1104 36-37 1.3058126005239714 1104 38-39 1.444187591640521 1104 40-41 1.5016404175878861 1104 42-43 1.4919459141954121 1104 44-45 1.4777663517050996 1104 46-47 1.7452920259001345 1104 48-49 2.0419893943171843 1104 50-51 2.1548196883291695 1104 52-53 2.1467745570995405 1104 54-55 2.234984654340515 1104 56-57 2.6146194226880795 1104 58-59 2.855469789965568 1104 60-61 2.899605087722801 1104 62-63 2.773583950872503 1104 64-65 2.7819136124024233 1104 66-67 2.9992960129357726 1104 68-69 3.214799384757278 1104 70-71 3.5869453749538636 1104 72-73 3.662835985160662 1104 74-75 3.697582526790022 1104 76-77 4.097167361219917 1104 78-79 4.268368455772251 1104 80-81 4.50033639517471 1104 82-83 4.802331473743024 1104 84-85 4.781082203823399 1104 86-87 5.01539129361775 1104 88-89 5.099637176446446 1104 90-91 5.285119174110115 1104 92-93 5.297022419721522 1104 94-95 5.391248209297711 1104 96-97 5.244465291673347 1104 98-99 4.959923998818875 1104 100-101 4.283587130564692 1105 1 0.3100961786400198 1105 2 0.2244387814149711 1105 3 0.23608932172739827 1105 4 0.12600729675401112 1105 5 0.18061444850571462 1105 6 0.3024166117358149 1105 7 0.227770737434831 1105 8 0.16661377562184043 1105 9 0.32412038820037026 1105 10-11 0.34050595280157836 1105 12-13 0.4095008724535205 1105 14-15 0.5345919444968246 1105 16-17 0.5041881170988205 1105 18-19 0.446733314702044 1105 20-21 0.45129867250164324 1105 22-23 0.5120270277079655 1105 24-25 0.6167230454861112 1105 26-27 0.6258718299380988 1105 28-29 0.6247142191996673 1105 30-31 0.6963502529360426 1105 32-33 0.8731196206569223 1105 34-35 1.0381139459661597 1105 36-37 1.027411573766102 1105 38-39 1.0699814597834276 1105 40-41 1.164694196313306 1105 42-43 1.2623245800622342 1105 44-45 1.4886715787372609 1105 46-47 1.6941706667717895 1105 48-49 1.6678044540121562 1105 50-51 1.9577901462188336 1105 52-53 1.9414702572792848 1105 54-55 2.021656363509827 1105 56-57 2.4172100627598923 1105 58-59 2.6337898541270732 1105 60-61 2.659805016432241 1105 62-63 2.7606304788268723 1105 64-65 3.057718402345241 1105 66-67 3.059797350720416 1105 68-69 3.0251627057669097 1105 70-71 3.3121170265256232 1105 72-73 3.5241247792852732 1105 74-75 3.6733967170879893 1105 76-77 3.9427836441564814 1105 78-79 3.9825215870142507 1105 80-81 3.9843348715564524 1105 82-83 4.220876309633468 1105 84-85 4.196554808036932 1105 86-87 4.405646765928541 1105 88-89 4.4366765862128155 1105 90-91 4.630919117164609 1105 92-93 4.649609908229181 1105 94-95 4.680376780074237 1105 96-97 4.578960900131712 1105 98-99 4.344651736278367 1105 100-101 3.5735264140511465 1106 1 0.48135260435475047 1106 2 0.46376535297533295 1106 3 0.4005329540093996 1106 4 0.2550651154314565 1106 5 0.3343213973884147 1106 6 0.355816813110458 1106 7 0.29209244122669276 1106 8 0.30430706768066074 1106 9 0.4710457806674597 1106 10-11 0.3896605229333616 1106 12-13 0.5026910465374144 1106 14-15 0.7115082574042759 1106 16-17 0.5688755542994031 1106 18-19 0.44961701199869 1106 20-21 0.6641018928298621 1106 22-23 0.584510703763442 1106 24-25 0.6625698758139222 1106 26-27 0.7366921764815686 1106 28-29 0.7774697406027542 1106 30-31 0.9636441068446757 1106 32-33 0.7149020465028428 1106 34-35 0.7215509861963127 1106 36-37 0.9379641149495086 1106 38-39 1.1768183057193298 1106 40-41 1.179703843345429 1106 42-43 0.9802384366989045 1106 44-45 1.1789117809865317 1106 46-47 1.3638954374139942 1106 48-49 1.270821793680355 1106 50-51 1.4963410505825436 1106 52-53 1.5466119744197684 1106 54-55 1.518935359010662 1106 56-57 1.5353560104337234 1106 58-59 1.9012880501893363 1106 60-61 2.3529093928424665 1106 62-63 2.1740518797237876 1106 64-65 2.507996099427391 1106 66-67 2.73465540450848 1106 68-69 2.5672264730136476 1106 70-71 2.937026829638704 1106 72-73 3.1087916729409315 1106 74-75 3.24235347360273 1106 76-77 3.0812346941888116 1106 78-79 3.4536299492678566 1106 80-81 3.44410679968216 1106 82-83 3.6119804236527457 1106 84-85 3.9161268296654406 1106 86-87 4.005015621997739 1106 88-89 4.319327703092366 1106 90-91 4.363608603513512 1106 92-93 4.4483911960064475 1106 94-95 4.363111768344737 1106 96-97 4.676532846791378 1106 98-99 4.5455924390487805 1106 100-101 3.3733476341898605 1107 1 0.5978507273415623 1107 2 0.47152445786079156 1107 3 0.4865834936017066 1107 4 0.3765017293362476 1107 5 0.392981128989895 1107 6 0.4779796756796486 1107 7 0.4467724346434352 1107 8 0.4006577987622748 1107 9 0.5225550978108444 1107 10-11 0.5564538477154173 1107 12-13 0.4860692261611774 1107 14-15 0.7324583108279654 1107 16-17 0.8190354335017815 1107 18-19 0.5860586286595648 1107 20-21 0.725938178616552 1107 22-23 0.7268558338481625 1107 24-25 0.8608249197153981 1107 26-27 0.8278416086752856 1107 28-29 0.8466026049222535 1107 30-31 1.0001903503365241 1107 32-33 0.8456861076958262 1107 34-35 1.1073835205690372 1107 36-37 1.2169938153850026 1107 38-39 1.2426548370721164 1107 40-41 1.4135717183366054 1107 42-43 1.3458574853131537 1107 44-45 1.6224864396104763 1107 46-47 1.697271396493278 1107 48-49 1.8646657912873934 1107 50-51 2.008925529237885 1107 52-53 2.1613004842776817 1107 54-55 2.2079998305602615 1107 56-57 2.3794617710485753 1107 58-59 2.598410093926219 1107 60-61 2.6352767022479107 1107 62-63 2.6687319776367424 1107 64-65 2.9758777522439566 1107 66-67 3.1220229882164716 1107 68-69 3.3025956973121495 1107 70-71 3.493640525434561 1107 72-73 3.514590820774334 1107 74-75 3.8483482905906783 1107 76-77 3.807631481975296 1107 78-79 4.047868289450047 1107 80-81 4.119454314892888 1107 82-83 4.136154185176721 1107 84-85 4.289132062313197 1107 86-87 4.727059750747518 1107 88-89 4.749479170349783 1107 90-91 4.70300889976027 1107 92-93 4.678842175527805 1107 94-95 4.795794395065986 1107 96-97 4.6760607851595175 1107 98-99 4.483090773640422 1107 100-101 3.48226677763442 1108 1 0.43838747959187074 1108 2 0.4631729117087353 1108 3 0.4953469041042453 1108 4 0.4257115476509199 1108 5 0.3636603730195276 1108 6 0.2769141427471098 1108 7 0.3832058222277155 1108 8 0.34120185828331984 1108 9 0.5275173989368298 1108 10-11 0.5646685486155434 1108 12-13 0.5333652038407877 1108 14-15 0.7159398931842205 1108 16-17 0.7419603346875547 1108 18-19 0.7702284230595424 1108 20-21 0.6743820620987933 1108 22-23 0.6861377284241783 1108 24-25 0.6519073922300933 1108 26-27 0.673143365741744 1108 28-29 0.7331592196630154 1108 30-31 0.9386711023211163 1108 32-33 0.8660767145314452 1108 34-35 0.9435398961627541 1108 36-37 1.165773833321019 1108 38-39 1.1728258936370324 1108 40-41 1.2907836624027667 1108 42-43 1.3171930551141955 1108 44-45 1.3147938764191878 1108 46-47 1.3723369958555551 1108 48-49 1.3326998033111792 1108 50-51 1.5125120705737913 1108 52-53 1.626288028702561 1108 54-55 1.7526140398803491 1108 56-57 2.2324959491466956 1108 58-59 2.1564098946370223 1108 60-61 2.2039789973952146 1108 62-63 2.2486469475772886 1108 64-65 2.511266200113223 1108 66-67 2.744961839646379 1108 68-69 2.6005330540730363 1108 70-71 2.8679242468282595 1108 72-73 3.0837063088668053 1108 74-75 3.0591942732525155 1108 76-77 3.2067738741100165 1108 78-79 3.126926315822651 1108 80-81 3.3822098202570885 1108 82-83 3.6334644452491283 1108 84-85 3.729361909192658 1108 86-87 4.132059252524513 1108 88-89 4.250967860687577 1108 90-91 4.264025938987029 1108 92-93 4.195585125672288 1108 94-95 4.2186130086774405 1108 96-97 4.387622880353323 1108 98-99 4.355151756136186 1108 100-101 3.581298896410784 1109 1 0.18371510514202782 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2311 42-43 0.9245713272667189 2311 44-45 0.9605270882230315 2311 46-47 1.001915106613815 2311 48-49 1.0664201732407577 2311 50-51 1.2826410118158265 2311 52-53 1.1554222471885893 2311 54-55 1.3822536894574142 2311 56-57 1.527046280353261 2311 58-59 1.3368068780737872 2311 60-61 1.4854027502181673 2311 62-63 1.7903853611529819 2311 64-65 2.0189917602035514 2311 66-67 1.9911430076067127 2311 68-69 2.096660752927601 2311 70-71 2.727273096458731 2311 72-73 3.107284268680793 2311 74-75 3.344969633341691 2311 76-77 3.1868082429597635 2311 78-79 3.4795826447418285 2311 80-81 3.313436939137315 2311 82-83 3.740879640692931 2311 84-85 4.135864704564472 2311 86-87 4.0119654348535825 2311 88-89 4.108559254541824 2311 90-91 4.342569326757058 2311 92-93 4.255970614122148 2311 94-95 4.450643383309512 2311 96-97 4.533183667353537 2311 98-99 4.121247466416074 2311 100-101 3.782988195042419 2312 1 -0.12095536273473684 2312 2 -0.032982499356727146 2312 3 -2.9290146096627723E-4 2312 4 0.038865870291864724 2312 5 -0.05827884616410017 2312 6 -0.08456862196513981 2312 7 -0.04960761269190783 2312 8 -0.05423952350295025 2312 9 0.01778711167253988 2312 10-11 -0.004442875283061198 2312 12-13 -0.045779556620338724 2312 14-15 -0.038134974466778715 2312 16-17 0.13833833141657692 2312 18-19 0.06938902551105741 2312 20-21 0.04245931174691009 2312 22-23 0.0796989280845466 2312 24-25 0.13164343503765963 2312 26-27 0.13221917365476088 2312 28-29 0.3090369969975768 2312 30-31 0.20479112534328436 2312 32-33 0.2333031695156862 2312 34-35 0.4664484400103035 2312 36-37 0.4204880791454073 2312 38-39 0.5609715259774859 2312 40-41 0.7750739925202197 2312 42-43 0.9659687199934126 2312 44-45 0.9171017922856848 2312 46-47 0.8705869687873999 2312 48-49 1.0940966337733897 2312 50-51 1.2841935439928633 2312 52-53 1.2400670272380339 2312 54-55 1.422861870154506 2312 56-57 1.6526164166563646 2312 58-59 1.634954255059398 2312 60-61 1.7361890690260857 2312 62-63 1.8756068240325554 2312 64-65 2.022992350224623 2312 66-67 2.289289894215763 2312 68-69 2.3424738688528137 2312 70-71 2.7045845501054977 2312 72-73 2.933892708689008 2312 74-75 2.5791126699063227 2312 76-77 2.8619226919442227 2312 78-79 3.164359571465649 2312 80-81 3.5895346323471493 2312 82-83 3.945991698601304 2312 84-85 4.3351265701569694 2312 86-87 4.271447797283834 2312 88-89 4.373415163275293 2312 90-91 4.322822745515296 2312 92-93 4.592363047180356 2312 94-95 4.532609236232243 2312 96-97 4.438818152829864 2312 98-99 4.570344908749261 2312 100-101 4.264637225479698 2313 1 -0.45408980837837376 2313 2 -0.30705329401043 2313 3 -0.28862480493788567 2313 4 -0.19056063087477781 2313 5 -0.39205696128935585 2313 6 -0.40388336959969706 2313 7 -0.2550357484082184 2313 8 -0.24182844474698584 2313 9 -0.2546300310979248 2313 10-11 -0.4143180985401145 2313 12-13 -0.3576975362360386 2313 14-15 -0.5337729292046589 2313 16-17 -0.30110411260225334 2313 18-19 -0.29176779668316044 2313 20-21 -0.33612949207472553 2313 22-23 -0.25004908945278004 2313 24-25 -0.2969713346768046 2313 26-27 -0.2714026985226283 2313 28-29 -0.2608826070548673 2313 30-31 -0.23303306205911412 2313 32-33 -0.09252337082919837 2313 34-35 -0.04918493768884247 2313 36-37 -0.01568217545892736 2313 38-39 0.056956129598255245 2313 40-41 0.27004346363440135 2313 42-43 0.17431762068945744 2313 44-45 0.397782718562965 2313 46-47 0.28611054624882115 2313 48-49 0.448723216517692 2313 50-51 0.42840500393150904 2313 52-53 0.5256027046369596 2313 54-55 0.6960443185218921 2313 56-57 0.9006564271663251 2313 58-59 1.042227832567633 2313 60-61 1.1320650897066535 2313 62-63 1.2985585383339462 2313 64-65 1.498418203039872 2313 66-67 1.665456931409075 2313 68-69 1.8663400170630986 2313 70-71 2.278936989953678 2313 72-73 2.4679645858011376 2313 74-75 2.5497728965071964 2313 76-77 2.724185007577489 2313 78-79 2.9863739104815146 2313 80-81 3.2803294471091107 2313 82-83 3.631473103257786 2313 84-85 3.9074181252770437 2313 86-87 3.9148798122464825 2313 88-89 3.9410013952281524 2313 90-91 3.772682728207201 2313 92-93 3.9719804674197405 2313 94-95 3.7555010033119096 2313 96-97 3.8234682556353228 2313 98-99 4.08613035500211 2313 100-101 3.9052559721988462 2314 1 -0.3874974304771399 2314 2 -0.28867950365832584 2314 3 -0.2500728644117558 2314 4 -0.2180878328848621 2314 5 -0.25098723878289775 2314 6 -0.18321627077242653 2314 7 -0.31983715989476735 2314 8 -0.14659578340553736 2314 9 -0.28414374326824543 2314 10-11 -0.2723297668764957 2314 12-13 -0.3818801365928337 2314 14-15 -0.31060849566542004 2314 16-17 -0.23594000764793321 2314 18-19 -0.35772354129409933 2314 20-21 -0.3208985986630353 2314 22-23 -0.29268637634135075 2314 24-25 -0.2507188100195421 2314 26-27 -0.17518571103921943 2314 28-29 -0.28415534861389347 2314 30-31 -0.11459792990189754 2314 32-33 -0.01326904431222431 2314 34-35 -0.008187539581420822 2314 36-37 0.20242383603797265 2314 38-39 0.193219851143958 2314 40-41 0.22134226697811243 2314 42-43 0.2869017982539006 2314 44-45 0.30333107144174676 2314 46-47 0.4028075713052104 2314 48-49 0.633180703164463 2314 50-51 0.7284404518159775 2314 52-53 0.918114361365717 2314 54-55 1.0982533378208998 2314 56-57 1.3461703500595874 2314 58-59 1.4321258105203043 2314 60-61 1.5984972824574335 2314 62-63 1.8138672920863392 2314 64-65 1.9391496973690998 2314 66-67 1.9408385988704175 2314 68-69 2.3839026133840164 2314 70-71 2.7029734235929155 2314 72-73 2.985956485468442 2314 74-75 3.0966794658841543 2314 76-77 3.3595929796906248 2314 78-79 3.7120075525775285 2314 80-81 3.9012898615547016 2314 82-83 4.032734672929401 2314 84-85 4.325359844311929 2314 86-87 4.50172561127037 2314 88-89 4.605210103800115 2314 90-91 4.664875800422664 2314 92-93 4.85192267933202 2314 94-95 4.784319686936417 2314 96-97 4.7564051441146695 2314 98-99 4.77152082639647 2314 100-101 4.194093549456484 2315 1 -0.5076089377838571 2315 2 -0.30769139639695453 2315 3 -0.46062861462989346 2315 4 -0.32653057803948116 2315 5 -0.2637810361460211 2315 6 -0.09806570499135603 2315 7 -0.286438900865285 2315 8 -0.22193741623527075 2315 9 -0.31548155613108975 2315 10-11 -0.2910584255597115 2315 12-13 -0.1742486191178685 2315 14-15 -0.32086933207327917 2315 16-17 -0.21080500342261388 2315 18-19 -0.2649186138116377 2315 20-21 -0.2821748866995293 2315 22-23 -0.15161417484497264 2315 24-25 -0.18716411097934582 2315 26-27 -0.12971900177579698 2315 28-29 -0.16717590498245727 2315 30-31 -0.056142302664703436 2315 32-33 -0.014968963928026824 2315 34-35 0.10569917366685644 2315 36-37 0.09232033279855756 2315 38-39 0.20992172883631355 2315 40-41 0.13376918262967052 2315 42-43 0.07070850518136496 2315 44-45 0.12861310316945662 2315 46-47 0.3907837789575055 2315 48-49 0.5823602003853665 2315 50-51 0.6187316555864726 2315 52-53 0.6925800125807555 2315 54-55 0.8944337144586427 2315 56-57 1.1401873956312514 2315 58-59 1.3781519456894173 2315 60-61 1.5389483958128913 2315 62-63 1.7440977611315525 2315 64-65 1.9608250894996893 2315 66-67 2.0291773942067586 2315 68-69 2.4306382936610333 2315 70-71 2.799105020077995 2315 72-73 3.0417082494549526 2315 74-75 3.3135635821045852 2315 76-77 3.4624560654818595 2315 78-79 3.6331884444985754 2315 80-81 3.9200236730709435 2315 82-83 4.118598047279285 2315 84-85 4.330582240585404 2315 86-87 4.386512902322991 2315 88-89 4.461877697976728 2315 90-91 4.498093710296182 2315 92-93 4.683936372126983 2315 94-95 4.927412829546224 2315 96-97 4.932640793474686 2315 98-99 4.99013011111454 2315 100-101 4.713338006725758 2316 1 -0.6935014444921741 2316 2 -0.7246984851870515 2316 3 -0.5940280299048339 2316 4 -0.699420962115731 2316 5 -0.42113215152909333 2316 6 -0.49673805870996546 2316 7 -0.5211179028277186 2316 8 -0.4399437440817948 2316 9 -0.5312250780983305 2316 10-11 -0.6383390535384024 2316 12-13 -0.5026177968983134 2316 14-15 -0.6937887521541768 2316 16-17 -0.5031985714469656 2316 18-19 -0.6347552111923918 2316 20-21 -0.5260402797869617 2316 22-23 -0.6085697998199393 2316 24-25 -0.6427800747744428 2316 26-27 -0.41662276243901886 2316 28-29 -0.45825984907313 2316 30-31 -0.5480562759916339 2316 32-33 -0.3100688878336655 2316 34-35 -0.24354488897080984 2316 36-37 -0.21204107534754257 2316 38-39 -0.36608552015243134 2316 40-41 -0.24777192834793738 2316 42-43 -0.05244320293669347 2316 44-45 -0.013378966997549924 2316 46-47 0.3014970634302543 2316 48-49 0.44915001976139024 2316 50-51 0.4202525414849845 2316 52-53 0.43516882270530743 2316 54-55 0.8346856268299874 2316 56-57 1.0339556682893445 2316 58-59 1.1381383288044802 2316 60-61 1.1234241457427991 2316 62-63 1.3258140893785786 2316 64-65 1.6961759245351402 2316 66-67 2.040763761301953 2316 68-69 2.216859207594311 2316 70-71 2.456166776656154 2316 72-73 2.9319161071985533 2316 74-75 2.914796110439717 2316 76-77 3.1592060354446154 2316 78-79 3.653027044362407 2316 80-81 3.6393906881696623 2316 82-83 3.8077295703679113 2316 84-85 3.9957250176289385 2316 86-87 4.370079524935029 2316 88-89 4.639930483430689 2316 90-91 4.852586281083958 2316 92-93 5.076454094102232 2316 94-95 5.238917094835184 2316 96-97 5.180297888272236 2316 98-99 4.874015128536145 2316 100-101 4.620998505744543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6652.0 3 2319.0 4 2336.0 5 2697.0 6 3151.0 7 3990.0 8 4885.0 9 6306.0 10 7834.0 11 9674.0 12 12230.0 13 15108.0 14 17349.0 15 21035.0 16 25072.0 17 31676.0 18 32106.0 19 29375.0 20 31263.0 21 34657.0 22 38105.0 23 43088.0 24 47005.0 25 51999.0 26 56430.0 27 61675.0 28 66983.0 29 72983.0 30 80257.0 31 88999.0 32 101564.0 33 117387.0 34 140493.0 35 175120.0 36 211787.0 37 233063.0 38 129746.0 39 8550.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98229298857868 18.588941545050762 14.559456297588833 22.869309168781726 2 15.503328785623044 19.744801334873166 40.41300849312064 24.338861386383147 3 19.281907469413305 24.19366393593679 29.458681097489574 27.065747497160324 4 12.75194675461851 16.50025838159256 36.72160692600998 34.02618793777894 5 13.919902990580225 37.292195910404644 33.68072970532994 15.107171393685196 6 32.3223458861804 35.62198952051722 17.246526768931556 14.809137824370827 7 29.056179875188764 32.85401590743044 19.961004516540477 18.128799700840325 8 26.791760318126805 34.61733395904859 19.294983574974683 19.29592214784993 9 26.874866001606502 16.627656873216033 19.997055740367678 36.5004213848098 10-11 25.456186846544664 26.51727604373782 26.521647849787712 21.504889259929804 12-13 26.294365791204612 23.85186839145857 27.369911350543873 22.483854466792945 14-15 22.990920759602528 25.22374556886587 25.971626416709498 25.813707254822106 16-17 22.273097765680497 28.540727055371406 26.54653638487796 22.639638794070134 18-19 22.181969246485043 26.86728883694532 29.15229011385489 21.798451802714755 20-21 22.99416642870623 25.916361764418177 29.307495605554145 21.781976201321445 22-23 22.690817805413705 25.78429948755642 29.29711156490409 22.227771142125786 24-25 22.458550057992248 26.025585758734966 29.336739168296088 22.179125014976698 26-27 22.152299436179607 26.361549054844346 29.277877614539527 22.20827389443652 28-29 22.00971840437958 26.564198500867086 29.38314338734286 22.042939707410476 30-31 22.202049110575743 26.15969249261355 29.312799246497228 22.325459150313478 32-33 22.015416445591892 26.22250644945514 29.306561301688273 22.455515803264696 34-35 22.658509529134275 26.32541752099986 28.970015652792075 22.04605729707379 36-37 22.546320425448876 26.15117465289013 28.973552710556934 22.32895221110406 38-39 22.233576952389324 26.41732304854913 28.97273004318365 22.3763699558779 40-41 22.444025943750347 26.205815414523126 29.01533470836 22.334823933366525 42-43 22.384892084554302 26.184047213223575 28.794500088161772 22.636560614060347 44-45 22.32629488771635 26.449128180839164 28.66860055794253 22.555976373501952 46-47 22.445197069274304 26.691109137624974 28.302153789011065 22.56154000408966 48-49 22.586883552036287 26.542749809351086 28.26832007681275 22.602046561799884 50-51 22.529763933215 26.84789254659551 28.079985539069703 22.542357981119792 52-53 22.70294109077117 26.95496135290274 27.805093781746002 22.537003774580082 54-55 22.423566881340104 26.810811718448473 27.97600424918581 22.789617151025617 56-57 22.46753734218101 26.973935049002662 27.944777428550545 22.613750180265786 58-59 22.688167184104355 26.623878977609305 27.9327950796133 22.755158758673037 60-61 22.51394745686246 26.752077888950264 28.176221083087654 22.55775357109962 62-63 22.404811074123067 26.831037789126338 28.150729802548707 22.613421334201885 64-65 22.24902867093737 26.63817171349509 28.11814285142801 22.994656764139524 66-67 22.322695411985897 26.778749969751043 27.9522120755824 22.946342542680664 68-69 22.34657605790137 26.613172265717118 28.072287817821067 22.96796385856044 70-71 22.4175997320951 26.686049928899504 27.902809395835916 22.99354094316949 72-73 22.522729652466477 26.60320111741777 27.79996522886715 23.0741040012486 74-75 22.406706222235723 26.932680214535498 27.61686462972031 23.043748933508464 76-77 22.517580187806807 26.65321179665976 27.648226843873214 23.18098117166022 78-79 22.58704396356326 26.705697209807568 27.55749942772818 23.14975939890099 80-81 22.57116907374638 26.9945885772902 27.45999463648347 22.974247712479958 82-83 22.67562547258944 26.818435272717757 27.384085632342575 23.121853622350226 84-85 22.799933229918725 26.504191156026174 27.416180583438155 23.279695030616946 86-87 22.745843450932053 26.497589801559258 27.422137083771002 23.334429663737687 88-89 22.734554137453085 26.43053103596123 27.378562558472442 23.456352268113243 90-91 22.80995082870063 26.445243522650657 27.402095769193725 23.342709879454993 92-93 22.995892188341955 26.157948988456937 27.299957178397165 23.54620164480394 94-95 22.73348768031503 26.087620150332707 27.482645843819757 23.696246325532506 96-97 22.84786538148556 25.957915414443246 27.404645589386956 23.789573614684237 98-99 22.864155870519834 25.926953104644934 26.975084257238517 24.233806767596715 100-101 23.5790627948557 25.05589666908999 27.09104217099326 24.273998365061054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1207.0 1 799.0 2 553.0 3 732.5 4 616.5 5 575.5 6 1100.0 7 2339.5 8 3731.5 9 4293.5 10 3593.5 11 2756.0 12 2633.0 13 2755.5 14 2911.5 15 3014.0 16 3170.5 17 3267.0 18 3336.0 19 3566.5 20 4024.5 21 4687.5 22 5382.0 23 6314.0 24 7492.5 25 9119.0 26 11099.0 27 13488.0 28 16046.0 29 18619.0 30 21670.5 31 24938.0 32 28567.0 33 31512.0 34 34057.0 35 37136.5 36 40992.0 37 45044.5 38 48265.5 39 50444.5 40 52684.5 41 56444.5 42 60710.5 43 64708.5 44 68098.5 45 70942.0 46 72139.5 47 74181.0 48 78701.0 49 79662.0 50 76960.5 51 74099.5 52 71858.5 53 71265.5 54 70835.0 55 67384.0 56 61360.5 57 56064.0 58 51576.0 59 45716.5 60 38860.0 61 32609.5 62 26750.5 63 21357.0 64 17126.5 65 13666.0 66 10519.0 67 8292.0 68 6509.5 69 4734.5 70 3557.5 71 2690.5 72 2093.5 73 1672.0 74 1217.5 75 904.0 76 648.0 77 413.5 78 255.5 79 154.5 80 94.5 81 60.0 82 32.5 83 19.5 84 18.5 85 13.5 86 6.5 87 7.0 88 8.0 89 5.0 90 3.0 91 4.0 92 2.5 93 0.5 94 1.5 95 2.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3787747845625818 2 0.024099360478036496 3 0.04686535470011605 4 0.04009975554951974 5 0.06069285661374355 6 0.05856934739129361 7 0.03027235240376306 8 0.029630361243487495 9 0.033186004592705994 10-11 0.03037112027457468 12-13 0.03103780340255315 14-15 0.0245191239289859 16-17 0.03061803995160374 18-19 0.02622286970048643 20-21 0.03970468406627324 22-23 0.026272253635892244 24-25 0.04802587718215265 26-27 0.04012444751722265 28-29 0.031926714239857774 30-31 0.03967999209857034 32-33 0.03693918368354775 34-35 0.03508728610582978 36-37 0.03629719252327218 38-39 0.01683992197338206 40-41 0.012938591076322873 42-43 0.012987975011728685 44-45 0.009358255759401466 46-47 0.016395466554729746 48-49 0.014123805526062374 50-51 0.0090866441146695 52-53 0.004395170251117311 54-55 0.004518630089631843 56-57 0.008000197535741623 58-59 0.0033827995752981556 60-61 0.00585199634558878 62-63 0.006000148151806217 64-65 0.007259438504654436 66-67 0.004296402380305687 68-69 0.006296451764241093 70-71 0.017333761327440182 72-73 0.013926269784439121 74-75 0.010049630855082842 76-77 0.007728585891009655 78-79 0.006197683893429467 80-81 0.007852045729524186 82-83 0.00992617101656831 84-85 0.004493938121928936 86-87 6.419911602755623E-4 88-89 0.024296896219659744 90-91 0.019506654485295934 92-93 0.013383046494975183 94-95 0.025037655250746933 96-97 0.0189881231635349 98-99 0.017111533618114027 100-101 0.11084224301834614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2024950.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.69476840678751 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48944642756702 41.2887778951237 2 13.525838695062356 14.795852298680009 3 4.715400988312155 7.7372329500261054 4 2.1190667937609047 4.636074700930656 5 1.1102772872913427 3.0363179547858135 6 0.664278715364919 2.1799542296665537 7 0.4686113330344306 1.7941411833179908 8 0.32206104507758054 1.4092043418692972 9 0.23532815041261657 1.1584096817774148 >10 1.2213546768199848 11.88589545148489 >50 0.08054267747606784 2.989921341952901 >100 0.04406219168574527 4.324053455910784 >500 0.0028210137117969116 1.0489550407400374 >1k 6.370030962122037E-4 0.49971571098599993 >5k 1.8200088463205818E-4 0.6967649639534873 >10k+ 9.100044231602909E-5 0.5187287987944313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10422 0.5146793747993778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8510 0.42025729030346426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5489 0.27106842144250476 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2033 0.10039754068001679 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.938393540581249E-5 0.0 12-13 0.0 0.0 0.0 9.876787081162498E-5 0.0 14-15 0.0 0.0 0.0 9.876787081162498E-5 4.938393540581249E-5 16-17 0.0 0.0 0.0 9.876787081162498E-5 4.938393540581249E-5 18-19 0.0 0.0 0.0 9.876787081162498E-5 4.938393540581249E-5 20-21 0.0 0.0 0.0 1.2345983851453122E-4 4.938393540581249E-5 22-23 0.0 0.0 0.0 3.2099558013778116E-4 4.938393540581249E-5 24-25 4.938393540581249E-5 0.0 0.0 9.13602805007531E-4 4.938393540581249E-5 26-27 4.938393540581249E-5 0.0 0.0 0.0020247413516383123 4.938393540581249E-5 28-29 9.876787081162498E-5 0.0 0.0 0.004765549766660906 4.938393540581249E-5 30-31 9.876787081162498E-5 0.0 0.0 0.015284328008098966 4.938393540581249E-5 32-33 9.876787081162498E-5 0.0 0.0 0.03748240697301168 4.938393540581249E-5 34-35 9.876787081162498E-5 0.0 0.0 0.07022395614706536 4.938393540581249E-5 36-37 1.9753574162324997E-4 0.0 0.0 0.1217807847107336 4.938393540581249E-5 38-39 2.4691967702906244E-4 0.0 0.0 0.20373342551667942 4.938393540581249E-5 40-41 2.4691967702906244E-4 0.0 0.0 0.2922047457961925 4.938393540581249E-5 42-43 2.4691967702906244E-4 0.0 0.0 0.3783550211116324 4.938393540581249E-5 44-45 2.9630361243487493E-4 0.0 0.0 0.4841107187831798 4.938393540581249E-5 46-47 2.9630361243487493E-4 0.0 0.0 0.6053729721721524 4.938393540581249E-5 48-49 2.9630361243487493E-4 0.0 0.0 0.7322896861650905 4.938393540581249E-5 50-51 2.9630361243487493E-4 0.0 0.0 0.8707375490752858 4.938393540581249E-5 52-53 2.9630361243487493E-4 0.0 0.0 0.9970616558433542 4.938393540581249E-5 54-55 2.9630361243487493E-4 0.0 0.0 1.1323242549198747 4.938393540581249E-5 56-57 2.9630361243487493E-4 0.0 0.0 1.2902787723153657 4.938393540581249E-5 58-59 2.9630361243487493E-4 0.0 0.0 1.4555421121509173 4.938393540581249E-5 60-61 2.9630361243487493E-4 0.0 0.0 1.6330032840317044 4.938393540581249E-5 62-63 2.9630361243487493E-4 0.0 0.0 1.808019951109904 4.938393540581249E-5 64-65 2.9630361243487493E-4 0.0 0.0 1.9853329711844738 4.938393540581249E-5 66-67 2.9630361243487493E-4 0.0 0.0 2.188300945702363 4.938393540581249E-5 68-69 2.9630361243487493E-4 0.0 0.0 2.407713770710388 4.938393540581249E-5 70-71 2.9630361243487493E-4 0.0 0.0 2.6343613422553647 4.938393540581249E-5 72-73 2.9630361243487493E-4 0.0 0.0 2.8637003382799575 4.938393540581249E-5 74-75 2.9630361243487493E-4 0.0 0.0 3.0956566828810588 4.938393540581249E-5 76-77 2.9630361243487493E-4 0.0 0.0 3.343366502876614 4.938393540581249E-5 78-79 2.9630361243487493E-4 0.0 0.0 3.6201140768907876 4.938393540581249E-5 80-81 2.9630361243487493E-4 0.0 0.0 3.90152843280081 4.938393540581249E-5 82-83 2.9630361243487493E-4 0.0 0.0 4.182251413615151 4.938393540581249E-5 84-85 2.9630361243487493E-4 0.0 0.0 4.471443739351589 4.938393540581249E-5 86-87 2.9630361243487493E-4 0.0 0.0 4.764018864663325 4.938393540581249E-5 88-89 2.9630361243487493E-4 0.0 0.0 5.077853774167263 4.938393540581249E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6225 0.0 40.399548 1 GTATCAA 10195 0.0 36.41645 1 TATCAAC 12610 0.0 29.167734 2 ATCAACG 12645 0.0 28.974262 3 TCAACGC 12740 0.0 28.720905 4 CAACGCA 12990 0.0 28.131573 5 AACGCAG 13220 0.0 27.637362 6 GTACATG 9105 0.0 25.68156 1 ACGCAGA 14755 0.0 24.501545 7 TACATGG 9355 0.0 24.280685 2 CGCAGAG 14960 0.0 24.038774 8 GAGTACT 7855 0.0 23.494781 12-13 GCAGAGT 15495 0.0 22.84031 9 ACATGGG 9995 0.0 22.29805 3 GTACTTT 8250 0.0 21.910051 14-15 GTAAGGT 610 0.0 21.812477 4 AGTACTT 8135 0.0 21.576624 12-13 AGAGTAC 12940 0.0 21.292452 10-11 GTAGCCC 795 0.0 19.725302 3 CATGGGG 5240 0.0 19.6791 4 >>END_MODULE