FastQCFastQC Report
Thu 26 May 2016
SRR938067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024950
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101940.5034198375268525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86000.4247018444899874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55570.2744265290501No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.10750882737845378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60600.044.81711
GTATCAA97650.036.7751961
ATCAACG120050.029.5531123
TATCAAC120800.029.3732622
TCAACGC120700.029.3545594
CAACGCA122700.028.7971425
AACGCAG126150.028.0482256
GTACATG91850.025.581621
TACATGG92400.024.966062
ACGCAGA142500.024.7634057
CGCAGAG143450.024.533198
GAGTACT79000.023.62663512-13
ACATGGG95900.023.2584513
GCAGAGT152600.022.6886869
GTACTTT83300.022.1789514-15
CATGGGA48100.020.6646374
AGAGTAC131350.020.46551910-11
CAGAGTA149300.020.0249910-11
GGACCGA4750.019.9973646
AGTACTT84350.019.96032112-13
Produced by FastQC (version 0.11.5)